RchiOBHm_Chr7g0203531

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014064
Physical Location & Seq
Forward (+)
21226401 .. 21231724
5324 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18218

Sequence Viewer

Length: 1755 bp
ATGGGCGTGGCCAAGTCGTGGAGGTTCAGCTTGACACCGGCGGCAGCGAAGCTGGCTCTGTTACAGCACCAGAACGGCTACAATGGCGGCGGTGCGGCCAAGCTGCAGAGCTTGAGATCGCCTAAAACGACGCCGTTTTCGCCGCGGCAGCGGAAGCAGCAGCAGCAGCCGCCGGCGACGAGGAGGTGGTCCGTAGTGTGTGGGCTGATGCTGTTTGGGCTGGGCCTGGTCTCGCTCTTCACCGGACACGTCGCCTCTGATCTCGAATGGTGGTACACTCAGCGGGTGGCCAATCACGGCTTGTACTTGAAGCTGACTGGAAATGGTCGAGAGGCGATTGATGTTTGGAAGTCCAAGTACTCGAAATTCTACTACGGTTGCAGTGAAAGAGGTCGGCATTATCCTCCGGCGAAAAAGGAGAAGGCATCGAATGGATATTTGCTGATTGCAACTAGTGGAGGGTTGAACCAGCAAAGAACAGGAATAACAGATGCAGTGGTTGTGGCCCGAATTCTTAATGCTACCTTAGTTGTGCCTGAGCTGGATCATCATTCCTATTGGAAGGATGATAGTGACTTTGTAAATATTTTTGATGTCGAATGGTTCATGTCTTCTCTTGCGAAAGATGTGACAATTGTTCGGAGAGTTCCTGAAAAAGTCATGAGGAGCATGGATAAACCTCCATATACCATGCGCGTTCCGAGGAAATCTGAGCCTGAATACTATATTGATCAAGTTTTACCAATACTTTTGAGGAGACGTGTTCTGCAACTGACAAAGTTTGATTATAGACTTTCGAGTAACCTTGATGACGAGCTGCAAAAGTTGCGTTGTCGTGTTAATTATCATGCACTTAGATTTACAAAATCTATCAATGAGCTGGGTCAGAAACTTGTTATGAGAATGCGACAGATGGCAACACGTTTCGTTGCTGTCCACTTGAGGTTTGAACCTGATATGCTAGCATTTTCTGGGTGTTACTATGGTGGGGGTGATAAAGAGAGATATGAGCTTGCTGAAATAAGAAAACGATGGGCAACTTTACCTGATTTAAGCCCTCTTGATGAGCGAAAACGAGGAAAATGTCCGCTGACGCCTCATGAAGTTGGTCTAATGCTGCGTGCACTTGGTTTCACAAATGACACATACCTCTATGTTGCTTCTGGTGAAATATATGGGGGAGAAGAGACTCTAAGGCCTCTAAGAGAACTGTTCCCAAACTTTTACACAAAGGAGATGCTTGCTAATGAAGAGCTAAAACCTTTTCTTCCTTTCTCTTCTCGCCTCGCTGCAGTTGACTACATTGTCTCTGACGAAAGTGATGTGTTTGTCACTAATAATAACGGGAATATGGCCAAGATTCTCGCAGGTCGAAGGAGGTATATGGGTCATAAGAGGACAATCCGGCCAAATGCAAAGAGGCTCAGTGCTTTGTTCATGGCAAGGGATCAGATGGATTGGGATACATTTGCAAAAAAAGTGAAGTCATGCCAAAGAGGATTCATGGGTGAACCTGAGGATATGAAACTTGGACGAGGTGAATTTCATGAATTTCCATACGCTTGTATCTGCGAGAAACCATCAAGTAGTATTGAAAACAAAGATCATCACACACAAGTTGTAAAGGACTCGACACGGAAAACCAGAAATCCATATAACGTATATTCAAGGGAGAAGGCTCTACACGTATCTATGAAGAGGAACATGGGAAAATCTGAAAGAGATCATGAGAATGATGAAGTTTTCCCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

584

Amino Acids

67.32

Weight (kDa)

9.58

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 146 - 462 8.4e-104 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16650 AT4G16650
fragaria_vesca FvH4_5g19080 FvH4_5g19080 FvH4_5g19080
malus_domestica MD04G1062900.v1.1 MD06G1057200.v1.1
prunus_persica Prupe.5G068100_v2.0.a1
pyrus_communis pycom04g05660 pycom06g04800
rosa_chinensis RchiOBHm_Chr7g0203531
rosa_laevigata RLG00000003522
rosa_multiflora Rmu_ssc0000362.1_g000007
rosa_roxburghii Rroxscaffold_3G00253730
rosa_rugosa Rorug07G0076700
rosa_samantha Rh7BG205900
rosa_wichuraiana Rw7G018010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1304
Acc36I ACCTGC 1 cut(s) 1358
AccB7I CCANNNNNTGG 1 cut(s) 18
AccII CGCG 2 cut(s) 145, 696
AclWI GGATC 2 cut(s) 552, 1455
AcoI YGGCCR 5 cut(s) 9, 96, 288, 1353, 1406
AcsI RAATTY 4 cut(s) 365, 510, 1541, 1550
AcyI GRCGYC 2 cut(s) 131, 1094
AfaI GTAC 3 cut(s) 275, 305, 359
AfiI CCNNNNNNNGG 1 cut(s) 18
AflIII ACRYGT 4 cut(s) 247, 760, 920, 1684
AgsI TTSAA 5 cut(s) 310, 466, 950, 1595, 1668
AhlI ACTAGT 1 cut(s) 452
AjiI CACGTC 2 cut(s) 250, 761
AjnI CCWGG 1 cut(s) 225
Alw21I GWGCWC 1 cut(s) 1126
Alw26I GTCTC 4 cut(s) 235, 751, 1181, 1312
Alw44I GTGCAC 1 cut(s) 1122
AlwI GGATC 2 cut(s) 552, 1455
AoxI GGCC 8 cut(s) 9, 96, 223, 288, 504, 1196, 1353, 1406
ApaLI GTGCAC 1 cut(s) 1122
ApoI RAATTY 4 cut(s) 365, 510, 1541, 1550
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
AspLEI GCGC 1 cut(s) 696
AspS9I GGNCC 3 cut(s) 189, 223, 505
AsuHPI GGTGA 5 cut(s) 232, 1004, 1178, 1520, 1550
AsuNHI GCTAGC 1 cut(s) 961
AvaII GGWCC 1 cut(s) 189
AxyI CCTNAGG 1 cut(s) 1515
BaeGI GKGCMC 1 cut(s) 1126
BaeI ACNNNNGTAYC 2 cut(s) 1549, 1582
BalI TGGCCA 3 cut(s) 11, 290, 1355
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 1126
BccI CCATC 4 cut(s) 907, 1026, 1447, 1588
BceAI ACGGC 3 cut(s) 91, 118, 313
BciT130I CCWGG 1 cut(s) 227
BciVI GTATCC 1 cut(s) 1456
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 4 cut(s) 235, 751, 1181, 1312
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 3 cut(s) 453, 962, 1753
BfmI CTRYAG 2 cut(s) 104, 1290
BfuAI ACCTGC 1 cut(s) 1358
BfuI GTATCC 1 cut(s) 1456
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 189
BmgBI CACGTC 2 cut(s) 250, 761
BmgT120I GGNCC 3 cut(s) 189, 223, 505
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 4 cut(s) 198, 434, 481, 1227
BmtI GCTAGC 1 cut(s) 965
BpiI GAAGAC 1 cut(s) 603
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 133, 961
BsaAI YACGTR 1 cut(s) 1687
BsaHI GRCGYC 2 cut(s) 131, 1094
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 143, 701
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse118I RCCGGY 2 cut(s) 37, 172
Bse1I ACTGG 1 cut(s) 322
Bse21I CCTNAGG 1 cut(s) 1515
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 143, 701
BseGI GGATG 1 cut(s) 571
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 5 cut(s) 293, 528, 702, 1438, 1506
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 3 cut(s) 196, 679, 769
BseSI GKGCMC 1 cut(s) 1126
BseYI CCCAGC 2 cut(s) 220, 880
Bsh1236I CGCG 2 cut(s) 145, 696
BshFI GGCC 8 cut(s) 11, 98, 225, 290, 506, 1198, 1355, 1408
BsiHKAI GWGCWC 1 cut(s) 1126
BsiSI CCGG 5 cut(s) 38, 173, 243, 407, 1405
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 4 cut(s) 235, 751, 1181, 1312
BsmBI CGTCTC 1 cut(s) 751
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 8 cut(s) 11, 98, 225, 290, 506, 1198, 1355, 1408
Bso31I GGTCTC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 1126
Bsp143I GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
BspANI GGCC 8 cut(s) 11, 98, 225, 290, 506, 1198, 1355, 1408
BspCNI CTCAG 5 cut(s) 292, 529, 703, 1437, 1507
BspFNI CGCG 2 cut(s) 145, 696
BspHI TCATGA 4 cut(s) 660, 1099, 1546, 1726
BspMAI CTGCAG 2 cut(s) 108, 1294
BspMI ACCTGC 1 cut(s) 1358
BspOI GCTAGC 1 cut(s) 965
BspPI GGATC 2 cut(s) 552, 1455
BspQI GCTCTTC 2 cut(s) 242, 1245
BspTNI GGTCTC 1 cut(s) 235
BsrFI RCCGGY 2 cut(s) 37, 172
BsrI ACTGG 1 cut(s) 322
BssAI RCCGGY 2 cut(s) 37, 172
BssECI CCNNGG 2 cut(s) 143, 701
BssMI GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
BssNI GRCGYC 2 cut(s) 131, 1094
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 377, 1212
Bst6I CTCTTC 5 cut(s) 242, 1179, 1245, 1282, 1691
BstACI GRCGYC 2 cut(s) 131, 1094
BstAPI GCANNNNNTGC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 1687
BstC8I GCNNGC 6 cut(s) 54, 174, 963, 1014, 1122, 1242
BstDEI CTNAG 9 cut(s) 279, 526, 537, 711, 854, 1193, 1202, 1424, 1515
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 571
BstFNI CGCG 2 cut(s) 145, 696
BstHHI GCGC 1 cut(s) 696
BstKTI GATC 7 cut(s) 119, 262, 547, 733, 1450, 1606, 1726
BstMAI GTCTC 4 cut(s) 235, 751, 1181, 1312
BstMBI GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 2 cut(s) 104, 1290
BstSLI GKGCMC 1 cut(s) 1126
BstUI CGCG 2 cut(s) 145, 696
BstV2I GAAGAC 1 cut(s) 603
Bsu36I CCTNAGG 1 cut(s) 1515
BsuI GTATCC 1 cut(s) 1456
BsuRI GGCC 8 cut(s) 11, 98, 225, 290, 506, 1198, 1355, 1408
BtgI CCRYGG 1 cut(s) 143
BtrI CACGTC 2 cut(s) 250, 761
BtsCI GGATG 1 cut(s) 571
BtsI GCAGTG 2 cut(s) 388, 501
BtsIMutI CAGTG 3 cut(s) 388, 501, 1432
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 6 cut(s) 54, 174, 963, 1014, 1122, 1242
CciI TCATGA 4 cut(s) 660, 1099, 1546, 1726
CfoI GCGC 1 cut(s) 696
Cfr10I RCCGGY 2 cut(s) 37, 172
Cfr13I GGNCC 3 cut(s) 189, 223, 505
Cfr42I CCGCGG 1 cut(s) 146
CseI GACGC 2 cut(s) 139, 1102
Csp6I GTAC 3 cut(s) 274, 304, 358
CviQI GTAC 3 cut(s) 274, 304, 358
DdeI CTNAG 9 cut(s) 279, 526, 537, 711, 854, 1193, 1202, 1424, 1515
DpnI GATC 7 cut(s) 118, 261, 546, 732, 1449, 1605, 1725
DpnII GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
DrdI GACNNNNNNGTC 1 cut(s) 1304
DseDI GACNNNNNNGTC 1 cut(s) 1304
EaeI YGGCCR 5 cut(s) 9, 96, 288, 1353, 1406
Eam1104I CTCTTC 5 cut(s) 242, 1179, 1245, 1282, 1691
EarI CTCTTC 5 cut(s) 242, 1179, 1245, 1282, 1691
Eco147I AGGCCT 1 cut(s) 1198
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 189
Eco81I CCTNAGG 1 cut(s) 1515
EcoRI GAATTC 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 751
FauI CCCGC 1 cut(s) 276
FbaI TGATCA 1 cut(s) 730
FokI GGATG 1 cut(s) 578
FspBI CTAG 3 cut(s) 453, 962, 1753
GlaI GCGC 1 cut(s) 695
GsaI CCCAGC 2 cut(s) 224, 884
HaeIII GGCC 8 cut(s) 11, 98, 225, 290, 506, 1198, 1355, 1408
HapII CCGG 5 cut(s) 38, 173, 243, 407, 1405
HgaI GACGC 2 cut(s) 139, 1102
HhaI GCGC 1 cut(s) 696
Hin1I GRCGYC 2 cut(s) 131, 1094
Hin6I GCGC 1 cut(s) 694
HinP1I GCGC 1 cut(s) 694
HincII GTYRAC 1 cut(s) 1297
HindII GTYRAC 1 cut(s) 1297
HinfI GANTC 4 cut(s) 1189, 1360, 1500, 1628
HpaII CCGG 5 cut(s) 38, 173, 243, 407, 1405
HphI GGTGA 5 cut(s) 232, 1004, 1178, 1520, 1550
Hpy166II GTNNAC 5 cut(s) 276, 937, 1124, 1297, 1511
Hpy188I TCNGA 8 cut(s) 259, 642, 702, 712, 888, 1312, 1452, 1717
Hpy188III TCNNGA 8 cut(s) 263, 329, 650, 661, 1061, 1100, 1547, 1727
Hpy8I GTNNAC 5 cut(s) 276, 937, 1124, 1297, 1511
Hpy99I CGWCG 3 cut(s) 133, 181, 254
HpyAV CCTTC 4 cut(s) 415, 556, 1368, 1669
HpyCH4III ACNGT 2 cut(s) 377, 1212
HpyCH4IV ACGT 5 cut(s) 249, 760, 922, 1659, 1686
HpyF3I CTNAG 9 cut(s) 279, 526, 537, 711, 854, 1193, 1202, 1424, 1515
HpySE526I ACGT 5 cut(s) 249, 760, 922, 1659, 1686
Hsp92I GRCGYC 2 cut(s) 131, 1094
HspAI GCGC 1 cut(s) 694
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 730
KspI CCGCGG 1 cut(s) 146
Kzo9I GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
LguI GCTCTTC 2 cut(s) 242, 1245
LmnI GCTCC 1 cut(s) 666
LweI GCATC 4 cut(s) 198, 434, 481, 1227
MaeI CTAG 3 cut(s) 453, 962, 1753
MaeII ACGT 5 cut(s) 249, 760, 922, 1659, 1686
MaeIII GTNAC 6 cut(s) 60, 572, 628, 800, 977, 1330
MalI GATC 7 cut(s) 118, 261, 546, 732, 1449, 1605, 1725
MboI GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
MboII GAAGA 7 cut(s) 229, 603, 1196, 1259, 1262, 1269, 1708
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 1 cut(s) 1126
MlsI TGGCCA 3 cut(s) 11, 290, 1355
MluCI AATT 6 cut(s) 365, 510, 633, 841, 1541, 1550
MluNI TGGCCA 3 cut(s) 11, 290, 1355
MlyI GAGTC 2 cut(s) 1183, 1622
Mox20I TGGCCA 3 cut(s) 11, 290, 1355
MreI CGCCGGCG 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 172
MscI TGGCCA 3 cut(s) 11, 290, 1355
MseI TTAA 3 cut(s) 516, 840, 1052
MslI CAYNNNNRTG 1 cut(s) 1731
Msp20I TGGCCA 3 cut(s) 11, 290, 1355
MspA1I CMGCKG 4 cut(s) 145, 151, 283, 1090
MspI CCGG 5 cut(s) 38, 173, 243, 407, 1405
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 909
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 145, 696
NaeI GCCGGC 1 cut(s) 174
NdeII GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
NgoMIV GCCGGC 1 cut(s) 172
NheI GCTAGC 1 cut(s) 961
NmuCI GTSAC 3 cut(s) 572, 628, 1330
PagI TCATGA 4 cut(s) 660, 1099, 1546, 1726
PceI AGGCCT 1 cut(s) 1198
PciSI GCTCTTC 2 cut(s) 242, 1245
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 909
PdiI GCCGGC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 1360, 1500
PflMI CCANNNNNTGG 1 cut(s) 18
PleI GAGTC 2 cut(s) 1183, 1622
PpsI GAGTC 2 cut(s) 1183, 1622
Ppu21I YACGTR 1 cut(s) 1687
Psp6I CCWGG 1 cut(s) 225
PspFI CCCAGC 2 cut(s) 220, 880
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 3 cut(s) 189, 223, 505
PstI CTGCAG 2 cut(s) 108, 1294
RsaI GTAC 3 cut(s) 275, 305, 359
RsaNI GTAC 3 cut(s) 274, 304, 358
RseI CAYNNNNRTG 1 cut(s) 1731
SacII CCGCGG 1 cut(s) 146
SapI GCTCTTC 2 cut(s) 242, 1245
SaqAI TTAA 3 cut(s) 516, 840, 1052
Sau3AI GATC 7 cut(s) 116, 259, 544, 730, 1447, 1603, 1723
Sau96I GGNCC 3 cut(s) 189, 223, 505
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 2 cut(s) 1183, 1622
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 1126
SfaNI GCATC 4 cut(s) 198, 434, 481, 1227
SfcI CTRYAG 2 cut(s) 104, 1290
Sfr303I CCGCGG 1 cut(s) 146
SgrAI CRCCGGYG 2 cut(s) 37, 172
SgrBI CCGCGG 1 cut(s) 146
SinI GGWCC 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 1731
SmlI CTYRAG 2 cut(s) 112, 940
SmoI CTYRAG 2 cut(s) 112, 940
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 6 cut(s) 365, 510, 633, 841, 1541, 1550
SseBI AGGCCT 1 cut(s) 1198
SspI AATATT 1 cut(s) 586
SspMI CTAG 3 cut(s) 453, 962, 1753
StuI AGGCCT 1 cut(s) 1198
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 2 cut(s) 377, 1212
TaiI ACGT 5 cut(s) 252, 763, 925, 1662, 1689
TaqI TCGA 8 cut(s) 264, 328, 362, 428, 597, 797, 1372, 1631
TasI AATT 6 cut(s) 365, 510, 633, 841, 1541, 1550
TatI WGTACW 2 cut(s) 303, 357
TauI GCSGC 6 cut(s) 44, 90, 98, 145, 148, 172
TfiI GAWTC 2 cut(s) 1360, 1500
Tru1I TTAA 3 cut(s) 516, 840, 1052
Tru9I TTAA 3 cut(s) 516, 840, 1052
TscAI CASTG 3 cut(s) 388, 501, 1432
TseFI GTSAC 3 cut(s) 572, 628, 1330
Tsp45I GTSAC 3 cut(s) 572, 628, 1330
TspGWI ACGGA 2 cut(s) 181, 1651
TspRI CASTG 3 cut(s) 388, 501, 1432
Van91I CCANNNNNTGG 1 cut(s) 18
VneI GTGCAC 1 cut(s) 1122
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 4 cut(s) 365, 510, 1541, 1550
XspI CTAG 3 cut(s) 453, 962, 1753
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.