Rw7G018010

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014064
Physical Location & Seq
Forward (+)
19209216 .. 19214545
5330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018010.1

Sequence Viewer

Length: 1767 bp
ATGGGCGTGGCCAAGTCGTGGAGGTTCAGCTTGACACCGGCGGCAGCGAAGCTGGCTCTGTTACAGCACCAGAACGGCTACAATGGCGGCGGAGGCGGCGGTGCGGCCAAGCTGCAGAGCTTGAGATCGCCTAAAACGACGCCGTTTTCGCCGCGGCAGCGGAAGCAGCAGCAGCAGCAGCCGCCGGCGACGAGGAGGTGGTCCGTAGTGTGTGGGCTGATGCTGTTTGGGCTGGGCCTGGTGTCGCTCTTCACCGGACACGTCGCCTCTGATCTCGAATGGTGGTACACTCAGCGGGTGGCCAATCACGGCCTGTACTTGAAGCTGACTGGGAATGGTCGAGAGGCGATTGATGTGTGGAAGTCCAAGTACTCGAAATTCTACTACGGTTGCAGTGAAAGAGGTCGGCATTATCCTCCGGCGAAAAAGGAGAAGGCATCGAATGGATATTTGCTGATTGCAACCAGTGGAGGGTTGAACCAGCAAAGAACAGGAATAACAGATGCAGTGGTTGTGGCCCGAATTCTTAATGCTACCTTGGTTGTGCCTGAGCTGGATCATCATTCCTATTGGAAGGATGATAGTGACTTTGTAAATATTTTTGATGTCGAATGGTTCATGTCTTCTCTTGCGAAAGATGTGACAATTGTTCGGAGAGTTCCTGAAAAAGTTATGAGGAGCATGGATAAACCTCCATATACCATGCGTGTTCCGAGGAAATCTGAGCCTGAATACTATATTGATCAAGTTTTACCAATACTTTTGAGGAGACGTGTTCTGCAACTGACAAAGTTTGATTATAGACTTTCGAGTAACCTTGATGACGAGCTGCAAAAGTTGCGTTGTCGTGTTAATTATCATGCACTTAGATTTACAAAATCTATCAATGAGCTGGGTCAGAAACTTGTTATGAGAATGCAACAGATGGCAACACGTTTCGTTGCTGTCCACTTGAGGTTTGAACCTGATATGCTAGCATTTTCTGGGTGTTACTATGGTGGGGGTGATAAAGAGAGATATGAGCTTGCTGAAATAAGAAAACGATGGGCAACTTTACCTGATTTAAGCCCTCTTGATGAGCGAAAACGAGGAAAATGTCCGCTGACTCCTCATGAAGTTGGTCTAATGCTGCGTGCACTTGGTTTCACAAATGACACATACCTCTATGTTGCTTCTGGTGAAATATACGGGGGAGAAGAGACTCTAAGGCCTCTAAGAGAACTGTTCCCAAACTTTTACACAAAGGAGATGCTTGCTAATGAAGAGCTAAAACCTTTTCTTCCTTTCTCTTCTCGCCTCGCTGCAGTTGACTACATTGTCTCTGACGAAAGTGATGTGTTTGTCACTAATAATAACGGGAATATGGCCAAGATTCTCGCAGGTCGAAGGAGGTATATGGGTCATAAGAGGACAATCCGGCCAAATGCAAAGAGGCTCAGTGCTTTGTTCATGGCAAGGGATCAGATGGATTGGGATACATTTGCAAAAAAAGTAAAGTCATGCCAAAGAGGATTCATGGGTGAACCTGAGGATATGAAACTTGGACGAGGTGAATTTCATGAATTTCCATACGCTTGTATCTGCGAGAAACCATCAAGTAGTATTGAAAACAAAGATCATCACACACAAGTTGTAAAGGACTCGACACGGAAAACCAGAAATCCATATAACGTATATTCAAGGGAGAAGGCTCTACACGTATCTATGAAGAGGAACATGGGAAAATCTGAAAGAGATCATGAGAATGATGAAGTTTTCCCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

588

Amino Acids

67.6

Weight (kDa)

9.55

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 150 - 466 1.7e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16650 AT4G16650
fragaria_vesca FvH4_5g19080 FvH4_5g19080 FvH4_5g19080
malus_domestica MD04G1062900.v1.1 MD06G1057200.v1.1
prunus_persica Prupe.5G068100_v2.0.a1
pyrus_communis pycom04g05660 pycom06g04800
rosa_chinensis RchiOBHm_Chr7g0203531
rosa_laevigata RLG00000003522
rosa_multiflora Rmu_ssc0000362.1_g000007
rosa_roxburghii Rroxscaffold_3G00253730
rosa_rugosa Rorug07G0076700
rosa_samantha Rh7BG205900
rosa_wichuraiana Rw7G018010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1316
Acc36I ACCTGC 1 cut(s) 1370
AccB7I CCANNNNNTGG 1 cut(s) 18
AccII CGCG 1 cut(s) 154
AclWI GGATC 2 cut(s) 564, 1467
AcoI YGGCCR 5 cut(s) 9, 105, 300, 1365, 1418
AcsI RAATTY 4 cut(s) 377, 522, 1553, 1562
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 3 cut(s) 287, 317, 371
AfiI CCNNNNNNNGG 2 cut(s) 18, 471
AflIII ACRYGT 4 cut(s) 259, 772, 932, 1696
AgsI TTSAA 5 cut(s) 322, 478, 962, 1607, 1680
AjiI CACGTC 2 cut(s) 262, 773
AjnI CCWGG 1 cut(s) 237
Alw21I GWGCWC 1 cut(s) 1138
Alw26I GTCTC 3 cut(s) 763, 1193, 1324
Alw44I GTGCAC 1 cut(s) 1134
AlwI GGATC 2 cut(s) 564, 1467
AoxI GGCC 9 cut(s) 9, 105, 235, 300, 310, 516, 1208, 1365, 1418
ApaLI GTGCAC 1 cut(s) 1134
ApoI RAATTY 4 cut(s) 377, 522, 1553, 1562
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
AspS9I GGNCC 3 cut(s) 201, 235, 517
AsuHPI GGTGA 5 cut(s) 244, 1016, 1190, 1532, 1562
AsuNHI GCTAGC 1 cut(s) 973
AvaII GGWCC 1 cut(s) 201
AxyI CCTNAGG 1 cut(s) 1527
BaeGI GKGCMC 1 cut(s) 1138
BaeI ACNNNNGTAYC 2 cut(s) 1561, 1594
BalI TGGCCA 3 cut(s) 11, 302, 1367
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 1138
BccI CCATC 4 cut(s) 919, 1038, 1459, 1600
BceAI ACGGC 3 cut(s) 91, 127, 325
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 1468
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 3 cut(s) 763, 1193, 1324
BfaI CTAG 2 cut(s) 974, 1765
BfmI CTRYAG 2 cut(s) 113, 1302
BfuAI ACCTGC 1 cut(s) 1370
BfuI GTATCC 1 cut(s) 1468
BmcAI AGTACT 1 cut(s) 371
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 201
BmgBI CACGTC 2 cut(s) 262, 773
BmgT120I GGNCC 3 cut(s) 201, 235, 517
BmrFI CCNGG 1 cut(s) 239
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 4 cut(s) 210, 446, 493, 1239
BmtI GCTAGC 1 cut(s) 977
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 142, 973
BsaAI YACGTR 1 cut(s) 1699
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 152, 537, 713
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 471
Bse118I RCCGGY 2 cut(s) 37, 184
Bse1I ACTGG 2 cut(s) 334, 465
Bse21I CCTNAGG 1 cut(s) 1527
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 152, 537, 713
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 2 cut(s) 18, 471
BseMII CTCAG 5 cut(s) 305, 540, 714, 1450, 1518
BseNI ACTGG 2 cut(s) 334, 465
BseRI GAGGAG 4 cut(s) 208, 691, 781, 1098
BseSI GKGCMC 1 cut(s) 1138
BseYI CCCAGC 2 cut(s) 232, 892
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 9 cut(s) 11, 107, 237, 302, 312, 518, 1210, 1367, 1420
BsiHKAI GWGCWC 1 cut(s) 1138
BsiSI CCGG 5 cut(s) 38, 185, 255, 419, 1417
BslI CCNNNNNNNGG 2 cut(s) 18, 471
BsmAI GTCTC 3 cut(s) 763, 1193, 1324
BsmBI CGTCTC 1 cut(s) 763
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 9 cut(s) 11, 107, 237, 302, 312, 518, 1210, 1367, 1420
Bsp1286I GDGCHC 1 cut(s) 1138
Bsp143I GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
BspANI GGCC 9 cut(s) 11, 107, 237, 302, 312, 518, 1210, 1367, 1420
BspCNI CTCAG 5 cut(s) 304, 541, 715, 1449, 1519
BspFNI CGCG 1 cut(s) 154
BspHI TCATGA 3 cut(s) 1111, 1558, 1738
BspMAI CTGCAG 2 cut(s) 117, 1306
BspMI ACCTGC 1 cut(s) 1370
BspOI GCTAGC 1 cut(s) 977
BspPI GGATC 2 cut(s) 564, 1467
BspQI GCTCTTC 2 cut(s) 254, 1257
BsrFI RCCGGY 2 cut(s) 37, 184
BsrI ACTGG 2 cut(s) 334, 465
BssAI RCCGGY 2 cut(s) 37, 184
BssECI CCNNGG 3 cut(s) 152, 537, 713
BssMI GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 389, 1224
Bst6I CTCTTC 5 cut(s) 254, 1191, 1257, 1294, 1703
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 838
BstBAI YACGTR 1 cut(s) 1699
BstC8I GCNNGC 6 cut(s) 54, 186, 975, 1026, 1134, 1254
BstDEI CTNAG 8 cut(s) 291, 549, 723, 866, 1205, 1214, 1436, 1527
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 583
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 7 cut(s) 128, 274, 559, 745, 1462, 1618, 1738
BstMAI GTCTC 3 cut(s) 763, 1193, 1324
BstMBI GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 2 cut(s) 113, 1302
BstSLI GKGCMC 1 cut(s) 1138
BstUI CGCG 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 615
Bsu36I CCTNAGG 1 cut(s) 1527
BsuI GTATCC 1 cut(s) 1468
BsuRI GGCC 9 cut(s) 11, 107, 237, 302, 312, 518, 1210, 1367, 1420
BtgI CCRYGG 1 cut(s) 152
BtrI CACGTC 2 cut(s) 262, 773
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 2 cut(s) 400, 513
BtsIMutI CAGTG 4 cut(s) 400, 472, 513, 1444
BveI ACCTGC 1 cut(s) 1370
Cac8I GCNNGC 6 cut(s) 54, 186, 975, 1026, 1134, 1254
CciI TCATGA 3 cut(s) 1111, 1558, 1738
Cfr10I RCCGGY 2 cut(s) 37, 184
Cfr13I GGNCC 3 cut(s) 201, 235, 517
Cfr42I CCGCGG 1 cut(s) 155
CseI GACGC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 286, 316, 370
CviQI GTAC 3 cut(s) 286, 316, 370
DdeI CTNAG 8 cut(s) 291, 549, 723, 866, 1205, 1214, 1436, 1527
DpnI GATC 7 cut(s) 127, 273, 558, 744, 1461, 1617, 1737
DpnII GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
DrdI GACNNNNNNGTC 1 cut(s) 1316
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 5 cut(s) 9, 105, 300, 1365, 1418
Eam1104I CTCTTC 5 cut(s) 254, 1191, 1257, 1294, 1703
EarI CTCTTC 5 cut(s) 254, 1191, 1257, 1294, 1703
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 1210
Eco47I GGWCC 1 cut(s) 201
Eco81I CCTNAGG 1 cut(s) 1527
EcoRI GAATTC 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
Esp3I CGTCTC 1 cut(s) 763
FauI CCCGC 1 cut(s) 288
FbaI TGATCA 1 cut(s) 742
FokI GGATG 1 cut(s) 590
FspBI CTAG 2 cut(s) 974, 1765
GsaI CCCAGC 2 cut(s) 236, 896
HaeIII GGCC 9 cut(s) 11, 107, 237, 302, 312, 518, 1210, 1367, 1420
HapII CCGG 5 cut(s) 38, 185, 255, 419, 1417
HgaI GACGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 1309
HindII GTYRAC 1 cut(s) 1309
HinfI GANTC 5 cut(s) 1105, 1201, 1372, 1512, 1640
HpaII CCGG 5 cut(s) 38, 185, 255, 419, 1417
HphI GGTGA 5 cut(s) 244, 1016, 1190, 1532, 1562
Hpy166II GTNNAC 5 cut(s) 288, 949, 1136, 1309, 1523
Hpy188I TCNGA 8 cut(s) 271, 654, 714, 724, 900, 1324, 1464, 1729
Hpy188III TCNNGA 7 cut(s) 275, 341, 662, 1073, 1112, 1559, 1739
Hpy8I GTNNAC 5 cut(s) 288, 949, 1136, 1309, 1523
Hpy99I CGWCG 3 cut(s) 142, 193, 266
HpyAV CCTTC 4 cut(s) 427, 568, 1380, 1681
HpyCH4III ACNGT 2 cut(s) 389, 1224
HpyCH4IV ACGT 5 cut(s) 261, 772, 934, 1671, 1698
HpyF3I CTNAG 8 cut(s) 291, 549, 723, 866, 1205, 1214, 1436, 1527
HpySE526I ACGT 5 cut(s) 261, 772, 934, 1671, 1698
Hsp92I GRCGYC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 742
KspI CCGCGG 1 cut(s) 155
Kzo9I GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
LguI GCTCTTC 2 cut(s) 254, 1257
LmnI GCTCC 1 cut(s) 678
LweI GCATC 4 cut(s) 210, 446, 493, 1239
MaeI CTAG 2 cut(s) 974, 1765
MaeII ACGT 5 cut(s) 261, 772, 934, 1671, 1698
MaeIII GTNAC 6 cut(s) 60, 584, 640, 812, 989, 1342
MalI GATC 7 cut(s) 127, 273, 558, 744, 1461, 1617, 1737
MboI GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
MboII GAAGA 7 cut(s) 241, 615, 1208, 1271, 1274, 1281, 1720
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 1138
MlsI TGGCCA 3 cut(s) 11, 302, 1367
MluCI AATT 6 cut(s) 377, 522, 645, 853, 1553, 1562
MluNI TGGCCA 3 cut(s) 11, 302, 1367
MlyI GAGTC 3 cut(s) 1099, 1195, 1634
Mox20I TGGCCA 3 cut(s) 11, 302, 1367
MreI CGCCGGCG 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 184
MscI TGGCCA 3 cut(s) 11, 302, 1367
MseI TTAA 3 cut(s) 528, 852, 1064
MslI CAYNNNNRTG 1 cut(s) 1743
Msp20I TGGCCA 3 cut(s) 11, 302, 1367
MspA1I CMGCKG 4 cut(s) 154, 160, 295, 1102
MspI CCGG 5 cut(s) 38, 185, 255, 419, 1417
MspR9I CCNGG 1 cut(s) 239
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 154
NaeI GCCGGC 1 cut(s) 186
NdeII GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
NgoMIV GCCGGC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 973
NmuCI GTSAC 3 cut(s) 584, 640, 1342
PagI TCATGA 3 cut(s) 1111, 1558, 1738
PceI AGGCCT 1 cut(s) 1210
PciSI GCTCTTC 2 cut(s) 254, 1257
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PctI GAATGC 1 cut(s) 921
PdiI GCCGGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 1372, 1512
PflMI CCANNNNNTGG 1 cut(s) 18
PleI GAGTC 3 cut(s) 1099, 1195, 1634
PpsI GAGTC 3 cut(s) 1099, 1195, 1634
Ppu21I YACGTR 1 cut(s) 1699
Psp6I CCWGG 1 cut(s) 237
PspFI CCCAGC 2 cut(s) 232, 892
PspGI CCWGG 1 cut(s) 237
PspPI GGNCC 3 cut(s) 201, 235, 517
PstI CTGCAG 2 cut(s) 117, 1306
RsaI GTAC 3 cut(s) 287, 317, 371
RsaNI GTAC 3 cut(s) 286, 316, 370
RseI CAYNNNNRTG 1 cut(s) 1743
SacII CCGCGG 1 cut(s) 155
SapI GCTCTTC 2 cut(s) 254, 1257
SaqAI TTAA 3 cut(s) 528, 852, 1064
Sau3AI GATC 7 cut(s) 125, 271, 556, 742, 1459, 1615, 1735
Sau96I GGNCC 3 cut(s) 201, 235, 517
ScaI AGTACT 1 cut(s) 371
SchI GAGTC 3 cut(s) 1099, 1195, 1634
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 1138
SfaNI GCATC 4 cut(s) 210, 446, 493, 1239
SfcI CTRYAG 2 cut(s) 113, 1302
Sfr303I CCGCGG 1 cut(s) 155
SgrAI CRCCGGYG 2 cut(s) 37, 184
SgrBI CCGCGG 1 cut(s) 155
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 1743
SmlI CTYRAG 2 cut(s) 121, 952
SmoI CTYRAG 2 cut(s) 121, 952
Sse9I AATT 6 cut(s) 377, 522, 645, 853, 1553, 1562
SseBI AGGCCT 1 cut(s) 1210
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 974, 1765
StuI AGGCCT 1 cut(s) 1210
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 389, 1224
TaiI ACGT 5 cut(s) 264, 775, 937, 1674, 1701
TaqI TCGA 8 cut(s) 276, 340, 374, 440, 609, 809, 1384, 1643
TasI AATT 6 cut(s) 377, 522, 645, 853, 1553, 1562
TatI WGTACW 2 cut(s) 315, 369
TauI GCSGC 7 cut(s) 44, 90, 99, 107, 154, 157, 184
TfiI GAWTC 2 cut(s) 1372, 1512
Tru1I TTAA 3 cut(s) 528, 852, 1064
Tru9I TTAA 3 cut(s) 528, 852, 1064
TscAI CASTG 4 cut(s) 400, 472, 513, 1444
TseFI GTSAC 3 cut(s) 584, 640, 1342
Tsp45I GTSAC 3 cut(s) 584, 640, 1342
TspGWI ACGGA 2 cut(s) 193, 1663
TspRI CASTG 4 cut(s) 400, 472, 513, 1444
Van91I CCANNNNNTGG 1 cut(s) 18
VneI GTGCAC 1 cut(s) 1134
VpaK11BI GGWCC 1 cut(s) 201
XapI RAATTY 4 cut(s) 377, 522, 1553, 1562
XspI CTAG 2 cut(s) 974, 1765
ZrmI AGTACT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.