RLG00000003522

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014064
Physical Location & Seq
Reverse (-)
49600139 .. 49604934
4796 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003522

Sequence Viewer

Length: 1701 bp
ATGGGCGTGGCCAAGTCGTGGAGGTTCAGCTTGACACCGGCGGCAGCGAAGCTGGCTCTGTTACAGCACCAGAACGGCTACAATGGCGGCGGCGGCGGCGGTGGTGGTGTGGCCAAGCTGCAGAGCTTGAGATCCCCTAAAACGGGGCCGTTTTCGCCGCGGCAGCGGAAGCAGCAGCAGCAGCAGCAGCCGCCGGCGACGAGGCGGTGGTCCGTAGTGTGTGGGCTGATGCTGTTTGGGCTGGGCCTGGTGTCGCTCTTCACCGGACACGTCGCCTCTGATCTCGAATGGTGGTACACTCAGCGGGTGGCCAATCACGGCTTGTACTTGAAGCTGACTGGGAATGGTCGAGAGGCGATTGATGTTTGGAAGTCCAAGTACTCGAAATTCTACTACGGTTGCAGTGAAAGAGGTCGGCATTATCCCCCGGCGAAAAAAGAGAAGGCATCGAATGGATATTTGCTGATTGCAACTAGTGGAGGGTTGAACCAGCAAAGAACGGGATGCTTCTGGAAATCTGACTTTGTAAATATTTTTGATGTCGAATGGTTCATTACTTCTCTTGCGAAAGATGTGACAATTGTTCGGAGAGTTCCTGAAAAAGTCATGAGGAGCATGGATAAACCTCCATATACCATGCGTGTTCCGAGGAAATCTGAGCCTGAATACTATATTGATCAAGTTTTACCTATACTTTTGAGGAGACGTGTTCTGCAACTGACAAAGTTTGATTATAGACTTTCAAGTAACCTTGATGACGAGCTGCAAAAGTTGCGTTGTCGTGTTAATTATCATGCACTTAGATTTACAAAATCTATCAATGAGCTGGGTCAGAAACTTGTTATGAGAATGCGACAGATGGCAACACGTTTCGTTGCTGTTCATTTGAGGTTTGAACCTGATATGCTAGCATTTTCTGGGTGTTACTATGGTGGGGGTGATAAAGAGAGATATGAGCTTGCTGAAATAAGAAAACGATGGGCAACTTTACCTGATTTAAGCCCTCTTGATGAGCGAAAACGAGGAAAATGTCCGCTGACTCCTCATGAAGTTGGTCTAATGCTGCGTGCACTTGGTTTCACAAATGAAACATACCTCTATGTTGCTTCTGGTGAAATATATGGAGGAGAAGAGACTCTAAGGCCTCTAAGAGAATTGTTCCCAAACTTTTACACAAAGGAGATGCTTGCTAATGAAGAGCTAAAACCTTTTCTTCCTTTCTCTTCTCGCCTCGCTGCAGTTGACTACATTGTCTCTGACGAAAGTGATGTGTTTGTCACTAATAATAATGGGAATATGGCCAAGATTCTCGCAGGTCGAAGGAGGTATATGGGTCATAAGAGGACAATCCGGCCAAATGCAAAGAGGCTCAGTGCTTTGTTCATGGCAAGGGATCAGATGGATTGGGATACCTTTGCAAAAAAAGTGAAGTCATGCCAAAGAGGATTCATGGGTGAACCTGAAGATATGAAACTTGGACGAGGTGAATTTCATGAATTTCCATACGCTTGTATCTGCGAGAAACCATCAAGTAGTATTGAAAACAAAGATCATCACACGCAAGTTGTAAAGGACTCGACACGGAAAACCAGAAATCCATATAACGTATATTCAAGGGAGAAGGCTCTACACATATCTATGAAGAGGAACATGGGAAAATCTGAAAGAGATCATGAGAATGATGAAGTTTTCCCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

567

Amino Acids

65.17

Weight (kDa)

9.68

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 174 - 444 2.6e-80 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014064)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16650 AT4G16650
fragaria_vesca FvH4_5g19080 FvH4_5g19080 FvH4_5g19080
malus_domestica MD04G1062900.v1.1 MD06G1057200.v1.1
prunus_persica Prupe.5G068100_v2.0.a1
pyrus_communis pycom04g05660 pycom06g04800
rosa_chinensis RchiOBHm_Chr7g0203531
rosa_laevigata RLG00000003522
rosa_multiflora Rmu_ssc0000362.1_g000007
rosa_roxburghii Rroxscaffold_3G00253730
rosa_rugosa Rorug07G0076700
rosa_samantha Rh7BG205900
rosa_wichuraiana Rw7G018010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1250
Acc36I ACCTGC 1 cut(s) 1304
AccB7I CCANNNNNTGG 1 cut(s) 18
AccII CGCG 1 cut(s) 160
AclWI GGATC 2 cut(s) 126, 1401
AcoI YGGCCR 5 cut(s) 9, 111, 309, 1299, 1352
AcsI RAATTY 3 cut(s) 386, 1487, 1496
AcuI CTGAAG 1 cut(s) 1482
AfaI GTAC 3 cut(s) 296, 326, 380
AfiI CCNNNNNNNGG 3 cut(s) 18, 142, 143
AflIII ACRYGT 3 cut(s) 268, 706, 866
AgsI TTSAA 6 cut(s) 331, 487, 744, 896, 1541, 1614
AhlI ACTAGT 1 cut(s) 473
AjiI CACGTC 2 cut(s) 271, 707
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 9 cut(s) 30, 52, 118, 126, 334, 763, 826, 958, 1201
AluI AGCT 9 cut(s) 30, 52, 118, 126, 334, 763, 826, 958, 1201
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 3 cut(s) 697, 1127, 1258
Alw44I GTGCAC 1 cut(s) 1068
AlwI GGATC 2 cut(s) 126, 1401
AoxI GGCC 8 cut(s) 9, 111, 146, 244, 309, 1142, 1299, 1352
ApaLI GTGCAC 1 cut(s) 1068
ApoI RAATTY 3 cut(s) 386, 1487, 1496
AspS9I GGNCC 3 cut(s) 146, 210, 244
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 5 cut(s) 253, 950, 1124, 1466, 1496
AsuNHI GCTAGC 1 cut(s) 907
AvaII GGWCC 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 1072
BaeI ACNNNNGTAYC 2 cut(s) 1495, 1528
BalI TGGCCA 4 cut(s) 11, 113, 311, 1301
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
Bbv12I GWGCWC 1 cut(s) 1072
BccI CCATC 4 cut(s) 853, 972, 1393, 1534
BceAI ACGGC 3 cut(s) 91, 133, 334
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 1402
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 3 cut(s) 697, 1127, 1258
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 3 cut(s) 474, 908, 1699
BfmI CTRYAG 2 cut(s) 119, 1236
BfuAI ACCTGC 1 cut(s) 1304
BfuI GTATCC 1 cut(s) 1402
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 2 cut(s) 248, 428
Bme18I GGWCC 1 cut(s) 210
BmgBI CACGTC 2 cut(s) 271, 707
BmgT120I GGNCC 3 cut(s) 146, 210, 244
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 2 cut(s) 248, 428
BmrI ACTGGG 1 cut(s) 348
BmsI GCATC 4 cut(s) 219, 455, 494, 1173
BmtI GCTAGC 1 cut(s) 911
BmuI ACTGGG 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 158, 426, 647
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 142, 143
Bse118I RCCGGY 2 cut(s) 37, 193
Bse1I ACTGG 1 cut(s) 343
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 3 cut(s) 158, 426, 647
BseGI GGATG 1 cut(s) 509
BseLI CCNNNNNNNGG 3 cut(s) 18, 142, 143
BseMII CTCAG 3 cut(s) 314, 648, 1384
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 4 cut(s) 625, 715, 1032, 1140
BseSI GKGCMC 1 cut(s) 1072
BseYI CCCAGC 2 cut(s) 241, 826
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 8 cut(s) 11, 113, 148, 246, 311, 1144, 1301, 1354
BsiHKAI GWGCWC 1 cut(s) 1072
BsiSI CCGG 5 cut(s) 38, 194, 264, 428, 1351
BslI CCNNNNNNNGG 3 cut(s) 18, 142, 143
BsmAI GTCTC 3 cut(s) 697, 1127, 1258
BsmBI CGTCTC 1 cut(s) 697
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 8 cut(s) 11, 113, 148, 246, 311, 1144, 1301, 1354
Bsp1286I GDGCHC 1 cut(s) 1072
Bsp143I GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
BspANI GGCC 8 cut(s) 11, 113, 148, 246, 311, 1144, 1301, 1354
BspCNI CTCAG 3 cut(s) 313, 649, 1383
BspFNI CGCG 1 cut(s) 160
BspHI TCATGA 4 cut(s) 606, 1045, 1492, 1672
BspLI GGNNCC 1 cut(s) 147
BspMAI CTGCAG 2 cut(s) 123, 1240
BspMI ACCTGC 1 cut(s) 1304
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 2 cut(s) 126, 1401
BspQI GCTCTTC 2 cut(s) 263, 1191
BsrFI RCCGGY 2 cut(s) 37, 193
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 2 cut(s) 37, 193
BssECI CCNNGG 3 cut(s) 158, 426, 647
BssMI GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 398
Bst6I CTCTTC 5 cut(s) 263, 1125, 1191, 1228, 1637
BstAPI GCANNNNNTGC 1 cut(s) 772
BstC8I GCNNGC 6 cut(s) 54, 195, 909, 960, 1068, 1188
BstDEI CTNAG 6 cut(s) 300, 657, 800, 1139, 1148, 1370
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 509
BstFNI CGCG 1 cut(s) 160
BstKTI GATC 6 cut(s) 134, 283, 679, 1396, 1552, 1672
BstMAI GTCTC 3 cut(s) 697, 1127, 1258
BstMBI GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 246, 426
BstSFI CTRYAG 2 cut(s) 119, 1236
BstSLI GKGCMC 1 cut(s) 1072
BstUI CGCG 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BsuI GTATCC 1 cut(s) 1402
BsuRI GGCC 8 cut(s) 11, 113, 148, 246, 311, 1144, 1301, 1354
BtgI CCRYGG 1 cut(s) 158
BtrI CACGTC 2 cut(s) 271, 707
BtsCI GGATG 1 cut(s) 509
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 409, 1378
BveI ACCTGC 1 cut(s) 1304
Cac8I GCNNGC 6 cut(s) 54, 195, 909, 960, 1068, 1188
CciI TCATGA 4 cut(s) 606, 1045, 1492, 1672
Cfr10I RCCGGY 2 cut(s) 37, 193
Cfr13I GGNCC 3 cut(s) 146, 210, 244
Cfr42I CCGCGG 1 cut(s) 161
Csp6I GTAC 3 cut(s) 295, 325, 379
CviQI GTAC 3 cut(s) 295, 325, 379
DdeI CTNAG 6 cut(s) 300, 657, 800, 1139, 1148, 1370
DpnI GATC 6 cut(s) 133, 282, 678, 1395, 1551, 1671
DpnII GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
DrdI GACNNNNNNGTC 1 cut(s) 1250
DseDI GACNNNNNNGTC 1 cut(s) 1250
EaeI YGGCCR 5 cut(s) 9, 111, 309, 1299, 1352
Eam1104I CTCTTC 5 cut(s) 263, 1125, 1191, 1228, 1637
EarI CTCTTC 5 cut(s) 263, 1125, 1191, 1228, 1637
Eco147I AGGCCT 1 cut(s) 1144
Eco47I GGWCC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 1482
EcoRII CCWGG 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 697
FauI CCCGC 1 cut(s) 297
FbaI TGATCA 1 cut(s) 676
FokI GGATG 1 cut(s) 516
FspBI CTAG 3 cut(s) 474, 908, 1699
GsaI CCCAGC 2 cut(s) 245, 830
HaeIII GGCC 8 cut(s) 11, 113, 148, 246, 311, 1144, 1301, 1354
HapII CCGG 5 cut(s) 38, 194, 264, 428, 1351
HincII GTYRAC 1 cut(s) 1243
HindII GTYRAC 1 cut(s) 1243
HinfI GANTC 5 cut(s) 1039, 1135, 1306, 1446, 1574
HpaII CCGG 5 cut(s) 38, 194, 264, 428, 1351
HphI GGTGA 5 cut(s) 253, 950, 1124, 1466, 1496
Hpy166II GTNNAC 4 cut(s) 297, 1070, 1243, 1457
Hpy188I TCNGA 9 cut(s) 280, 520, 588, 648, 658, 834, 1258, 1398, 1663
Hpy188III TCNNGA 9 cut(s) 284, 350, 511, 596, 607, 1007, 1046, 1493, 1673
Hpy8I GTNNAC 4 cut(s) 297, 1070, 1243, 1457
Hpy99I CGWCG 2 cut(s) 202, 275
HpyAV CCTTC 3 cut(s) 436, 1314, 1615
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 4 cut(s) 270, 706, 868, 1605
HpyF3I CTNAG 6 cut(s) 300, 657, 800, 1139, 1148, 1370
HpySE526I ACGT 4 cut(s) 270, 706, 868, 1605
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 676
KspI CCGCGG 1 cut(s) 161
Kzo9I GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
LguI GCTCTTC 2 cut(s) 263, 1191
LmnI GCTCC 1 cut(s) 612
LweI GCATC 4 cut(s) 219, 455, 494, 1173
MaeI CTAG 3 cut(s) 474, 908, 1699
MaeII ACGT 4 cut(s) 270, 706, 868, 1605
MaeIII GTNAC 5 cut(s) 60, 574, 746, 923, 1276
MalI GATC 6 cut(s) 133, 282, 678, 1395, 1551, 1671
MboI GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
MboII GAAGA 7 cut(s) 250, 1142, 1205, 1208, 1215, 1475, 1654
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 1072
MlsI TGGCCA 4 cut(s) 11, 113, 311, 1301
MluCI AATT 6 cut(s) 386, 579, 787, 1154, 1487, 1496
MluNI TGGCCA 4 cut(s) 11, 113, 311, 1301
MlyI GAGTC 3 cut(s) 1033, 1129, 1568
Mox20I TGGCCA 4 cut(s) 11, 113, 311, 1301
MreI CGCCGGCG 1 cut(s) 193
MroNI GCCGGC 1 cut(s) 193
MscI TGGCCA 4 cut(s) 11, 113, 311, 1301
MseI TTAA 2 cut(s) 786, 998
MslI CAYNNNNRTG 2 cut(s) 1637, 1677
Msp20I TGGCCA 4 cut(s) 11, 113, 311, 1301
MspA1I CMGCKG 4 cut(s) 160, 166, 304, 1036
MspI CCGG 5 cut(s) 38, 194, 264, 428, 1351
MspR9I CCNGG 2 cut(s) 248, 428
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 160
NaeI GCCGGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 428
NdeII GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
NgoMIV GCCGGC 1 cut(s) 193
NheI GCTAGC 1 cut(s) 907
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 574, 1276
PagI TCATGA 4 cut(s) 606, 1045, 1492, 1672
PceI AGGCCT 1 cut(s) 1144
PciSI GCTCTTC 2 cut(s) 263, 1191
PctI GAATGC 1 cut(s) 855
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 1306, 1446
PflMI CCANNNNNTGG 1 cut(s) 18
PleI GAGTC 3 cut(s) 1033, 1129, 1568
PpsI GAGTC 3 cut(s) 1033, 1129, 1568
Psp6I CCWGG 1 cut(s) 246
PspFI CCCAGC 2 cut(s) 241, 826
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 146, 210, 244
PstI CTGCAG 2 cut(s) 123, 1240
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 3 cut(s) 296, 326, 380
RsaNI GTAC 3 cut(s) 295, 325, 379
RseI CAYNNNNRTG 2 cut(s) 1637, 1677
SacII CCGCGG 1 cut(s) 161
SapI GCTCTTC 2 cut(s) 263, 1191
SaqAI TTAA 2 cut(s) 786, 998
Sau3AI GATC 6 cut(s) 131, 280, 676, 1393, 1549, 1669
Sau96I GGNCC 3 cut(s) 146, 210, 244
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 3 cut(s) 1033, 1129, 1568
ScrFI CCNGG 2 cut(s) 248, 428
SduI GDGCHC 1 cut(s) 1072
SfaNI GCATC 4 cut(s) 219, 455, 494, 1173
SfcI CTRYAG 2 cut(s) 119, 1236
Sfr303I CCGCGG 1 cut(s) 161
SgrAI CRCCGGYG 2 cut(s) 37, 193
SgrBI CCGCGG 1 cut(s) 161
SinI GGWCC 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 1637, 1677
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 6 cut(s) 386, 579, 787, 1154, 1487, 1496
SseBI AGGCCT 1 cut(s) 1144
SspI AATATT 1 cut(s) 532
SspMI CTAG 3 cut(s) 474, 908, 1699
StuI AGGCCT 1 cut(s) 1144
StyD4I CCNGG 2 cut(s) 246, 426
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 4 cut(s) 273, 709, 871, 1608
TaqI TCGA 7 cut(s) 285, 349, 383, 449, 543, 1318, 1577
TasI AATT 6 cut(s) 386, 579, 787, 1154, 1487, 1496
TatI WGTACW 2 cut(s) 324, 378
TauI GCSGC 8 cut(s) 44, 90, 93, 96, 99, 160, 163, 193
TfiI GAWTC 2 cut(s) 1306, 1446
Tru1I TTAA 2 cut(s) 786, 998
Tru9I TTAA 2 cut(s) 786, 998
TscAI CASTG 2 cut(s) 409, 1378
TseFI GTSAC 2 cut(s) 574, 1276
Tsp45I GTSAC 2 cut(s) 574, 1276
TspGWI ACGGA 2 cut(s) 202, 1597
TspRI CASTG 2 cut(s) 409, 1378
Van91I CCANNNNNTGG 1 cut(s) 18
VneI GTGCAC 1 cut(s) 1068
VpaK11BI GGWCC 1 cut(s) 210
XapI RAATTY 3 cut(s) 386, 1487, 1496
XspI CTAG 3 cut(s) 474, 908, 1699
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.