RchiOBHm_Chr7g0214001

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0021482
Physical Location & Seq
Forward (+)
31157071 .. 31158974
1904 bp
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UTR
Exon/CDS
Intron
PRQ19151

Sequence Viewer

Length: 1455 bp
ATGTTTATTTATTGTTTTATCCCAATCTTGGAGACCCAGCTTGTCAAAGAAATTGTAGCAGAATGCTCTAAGCTTCGATGCGATGTATGCCATGATTGGAAAAGTTTTATCCCGGCAGATTCAAGTCAAGCTGAGTATGTGGAACATCCTTTCTGGGGACAAAATTACTGCCCCTCTCATGAGGGCACTACTCGAAAGTGTTGTAGCTGTGAAAGATTAGAACCAAAGGACACAAAGTATTTTGTACTTGACGATGGCCGGCACCTATGTCCAGAGTGTCAAGAATCAGCAATTATGGATTCTGACCAATGTCAATCCCTTTACCTTGAAGTACAAAAATTCTATGAAGATCACGATATGAAATTGGGACACCAAATTCGACTGCAGTTGGTTGGCAGACAAAAATTAAACCGGGTCATGAAGGGAGATCAGAGTAATTGTGATGGAGAAATTAGAGCAGTCTGCCTCGAAGCCAAAGAAATACTCATCTGGTATGGCCTTCCTAGGTTGTTAACAGGGTCAATTCTGGCTCAAGAGATGATCAAAGCATGGCTAATGCTTCAAGTCTATCCACATTTCAAATTGAGCGCTGAGGTTGAAAGAGGTATCTGCGGAGTGCTGGCCTATAGTTGGCTGGAGTACTACTCTTCTGATCCTGATTCATCTAAAATATGGTCTGACTTCGAGAAGAAGCTTGTTGAATTTATTAAACACCAAATTGAGTCATCAGATTATGGAGAAGTATTCAGGAAAGCTAACAAAGTGGTTCATGAGTATGGTCTATGTACTAGTGCGATTGAACACATTGTACGATCAATGTATTTCAAAGTGGTGAAACAGCTCCAGTATACCAGCATTTCTGCCCCTTCAGCCAGTTCGTGGAAGTATGATGTGTTTCTGAGTTTTAGAGGGGAAACCCGAATGGGTTTTACAAGCCATCTAGATAAAGCATTGAAGATGGAAGGCATCAACGTTTTCACGGACAGTCTAATCAAAAGAGGTGCGAGTATTCCTGCAACGCTCTTGACTGCAATCCTGAAATCAAGGTTTGCCATCATTGTCCTCTCGCAGAACTACACTTCTTCAATGTGGTGTTTAGATGAAGTTACAAAGGCTTTGCAATGCATGGACAAGAGGTCCGCAGTTTTCCCAATTTTTTACCATGTGGACCCCAGTGATGTACGATATGATAAGATGGGAGCCTATCAAAGGCATAGCACTCGCCGGTACCTAAAGAAAACAACAGTGCAAGACTGGACAATGAGCGTACGCGAAGTGACCAATGTTAGTGGCTGGGTTTCAAGTGGATCTCGTCGGGTGGAGGATGAGATCATCACACATGTTGTAAATGAATTGCGTAACAGAGTGGTTTGTGCTGGCTGCAACAGAAGGATTAATGAGACTTCATATTTGAGGTGCAACCATCGTGTTTGGCATCAAGAATGCTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

484

Amino Acids

56.19

Weight (kDa)

7.76

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 157 - 270 1.3e-26 Protein DA1
TIR PF01582 295 - 454 1e-34 TIR domain
TIR_2 PF13676 298 - 391 5.6e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021482)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0214001
rosa_samantha Rh7AG278600 Rh7CG297500 Rh7DG284100
rosa_wichuraiana Rw7G023960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1227
AccB1I GGYRCC 2 cut(s) 261, 1227
AccB7I CCANNNNNTGG 1 cut(s) 879
AccI GTMKAC 1 cut(s) 848
AccII CGCG 1 cut(s) 1272
AciI CCGC 3 cut(s) 612, 1140, 1450
AclI AACGTT 1 cut(s) 972
AclWI GGATC 2 cut(s) 647, 1315
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 3 cut(s) 338, 375, 701
AcuI CTGAAG 1 cut(s) 852
AfaI GTAC 8 cut(s) 246, 333, 641, 787, 810, 1182, 1229, 1269
AfeI AGCGCT 1 cut(s) 589
AfiI CCNNNNNNNGG 5 cut(s) 28, 155, 630, 879, 1209
AflIII ACRYGT 1 cut(s) 1339
AhdI GACNNNNNGTC 1 cut(s) 1135
AhlI ACTAGT 1 cut(s) 788
AluBI AGCT 7 cut(s) 40, 73, 131, 207, 694, 755, 841
AluI AGCT 7 cut(s) 40, 73, 131, 207, 694, 755, 841
Alw26I GTCTC 2 cut(s) 26, 1394
AlwI GGATC 2 cut(s) 647, 1315
Aor51HI AGCGCT 1 cut(s) 589
AoxI GGCC 3 cut(s) 256, 496, 621
ApeKI GCWGC 1 cut(s) 1380
ApoI RAATTY 3 cut(s) 338, 375, 701
ArsI GACNNNNNNTTYG 4 cut(s) 360, 392, 1393, 1425
AseI ATTAAT 1 cut(s) 1395
Asp718I GGTACC 1 cut(s) 1227
AspA2I CCTAGG 1 cut(s) 503
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 1137, 1168
AsuC2I CCSGG 2 cut(s) 113, 413
AsuHPI GGTGA 1 cut(s) 844
AvaII GGWCC 2 cut(s) 1137, 1168
AvrII CCTAGG 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 188
BanI GGYRCC 2 cut(s) 261, 1227
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 1367
BccI CCATC 7 cut(s) 248, 437, 945, 952, 1061, 1189, 1431
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BclI TGATCA 1 cut(s) 540
BcnI CCSGG 2 cut(s) 113, 413
BcoDI GTCTC 2 cut(s) 26, 1394
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 3 cut(s) 504, 789, 941
BfmI CTRYAG 2 cut(s) 383, 625
BfoI RGCGCY 1 cut(s) 591
BisI GCNGC 1 cut(s) 1381
BlnI CCTAGG 1 cut(s) 503
BlsI GCNGC 1 cut(s) 1382
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 113, 413
Bme18I GGWCC 2 cut(s) 1137, 1168
BmeRI GACNNNNNGTC 1 cut(s) 1135
BmgT120I GGNCC 2 cut(s) 1137, 1168
BmiI GGNNCC 4 cut(s) 263, 1170, 1201, 1229
BmrFI CCNGG 2 cut(s) 113, 413
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 3 cut(s) 68, 975, 1444
BmuI ACTGGG 1 cut(s) 1167
BoxI GACNNNNGTC 1 cut(s) 309
BplI GAGNNNNNCTC 2 cut(s) 629, 661
BpmI CTGGAG 2 cut(s) 656, 827
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 113, 413
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 503
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 155, 630, 879, 1209
Bse118I RCCGGY 2 cut(s) 258, 1224
Bse1I ACTGG 4 cut(s) 844, 873, 1173, 1259
Bse3DI GCAATG 1 cut(s) 1127
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 2 cut(s) 145, 1330
BseLI CCNNNNNNNGG 5 cut(s) 28, 155, 630, 879, 1209
BseMI GCAATG 1 cut(s) 1127
BseMII CTCAG 3 cut(s) 123, 582, 890
BseNI ACTGG 4 cut(s) 844, 873, 1173, 1259
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 1 cut(s) 1367
BseYI CCCAGC 2 cut(s) 36, 1293
Bsh1236I CGCG 1 cut(s) 1272
BshFI GGCC 3 cut(s) 258, 498, 623
BshNI GGYRCC 2 cut(s) 261, 1227
BsiSI CCGG 4 cut(s) 113, 259, 412, 1225
BsiWI CGTACG 1 cut(s) 1267
BslFI GGGAC 2 cut(s) 171, 381
BslI CCNNNNNNNGG 5 cut(s) 28, 155, 630, 879, 1209
BsmAI GTCTC 2 cut(s) 26, 1394
BsmFI GGGAC 2 cut(s) 171, 381
BsmI GAATGC 2 cut(s) 68, 1448
BsnI GGCC 3 cut(s) 258, 498, 623
Bso31I GGTCTC 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
BspACI CCGC 3 cut(s) 612, 1140, 1450
BspANI GGCC 3 cut(s) 258, 498, 623
BspCNI CTCAG 3 cut(s) 124, 583, 891
BspFNI CGCG 1 cut(s) 1272
BspHI TCATGA 3 cut(s) 178, 417, 769
BspLI GGNNCC 4 cut(s) 263, 1170, 1201, 1229
BspMAI CTGCAG 1 cut(s) 387
BspPI GGATC 2 cut(s) 647, 1315
BspT107I GGYRCC 2 cut(s) 261, 1227
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 1127
BsrFI RCCGGY 2 cut(s) 258, 1224
BsrI ACTGG 4 cut(s) 844, 873, 1173, 1259
BssAI RCCGGY 2 cut(s) 258, 1224
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
BssNAI GTATAC 1 cut(s) 849
BssT1I CCWWGG 1 cut(s) 503
Bst1107I GTATAC 1 cut(s) 849
Bst4CI ACNGT 2 cut(s) 986, 1246
Bst6I CTCTTC 1 cut(s) 652
BstC8I GCNNGC 3 cut(s) 260, 621, 1378
BstDEI CTNAG 4 cut(s) 69, 132, 591, 899
BstENI CCTNNNNNAGG 1 cut(s) 1207
BstF5I GGATG 2 cut(s) 145, 1330
BstFNI CGCG 1 cut(s) 1272
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 7 cut(s) 352, 430, 543, 655, 815, 1310, 1332
BstMAI GTCTC 2 cut(s) 26, 1394
BstMBI GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
BstMWI GCNNNNNNNGC 2 cut(s) 87, 869
BstNSI RCATGY 1 cut(s) 1343
BstPAI GACNNNNGTC 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 111, 411
BstSFI CTRYAG 2 cut(s) 383, 625
BstSLI GKGCMC 1 cut(s) 188
BstUI CGCG 1 cut(s) 1272
BstV1I GCAGC 1 cut(s) 1367
BstX2I RGATCY 1 cut(s) 1307
BstYI RGATCY 1 cut(s) 1307
BstZ17I GTATAC 1 cut(s) 849
BsuRI GGCC 3 cut(s) 258, 498, 623
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 2 cut(s) 145, 1330
BtsIMutI CAGTG 2 cut(s) 1180, 1251
Cac8I GCNNGC 3 cut(s) 260, 621, 1378
CciI TCATGA 3 cut(s) 178, 417, 769
CfoI GCGC 1 cut(s) 590
Cfr10I RCCGGY 2 cut(s) 258, 1224
Cfr13I GGNCC 2 cut(s) 1137, 1168
Csp6I GTAC 8 cut(s) 245, 332, 640, 786, 809, 1181, 1228, 1268
CspCI CAANNNNNGTGG 2 cut(s) 1270, 1305
CviAII CATG 8 cut(s) 92, 179, 418, 549, 770, 1126, 1163, 1340
CviQI GTAC 8 cut(s) 245, 332, 640, 786, 809, 1181, 1228, 1268
DdeI CTNAG 4 cut(s) 69, 132, 591, 899
DpnI GATC 7 cut(s) 351, 429, 542, 654, 814, 1309, 1331
DpnII GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
DriI GACNNNNNGTC 1 cut(s) 1135
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 652
Eam1105I GACNNNNNGTC 1 cut(s) 1135
EarI CTCTTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 503
Eco31I GGTCTC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 1137, 1168
Eco47III AGCGCT 1 cut(s) 589
Eco57I CTGAAG 1 cut(s) 852
EcoNI CCTNNNNNAGG 1 cut(s) 1207
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 1127
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 8 cut(s) 95, 182, 421, 552, 773, 1129, 1166, 1343
FaqI GGGAC 2 cut(s) 171, 381
FatI CATG 8 cut(s) 91, 178, 417, 548, 769, 1125, 1162, 1339
FbaI TGATCA 1 cut(s) 540
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 1 cut(s) 1381
FokI GGATG 2 cut(s) 132, 1337
Fsp4HI GCNGC 1 cut(s) 1381
FspBI CTAG 3 cut(s) 504, 789, 941
GlaI GCGC 1 cut(s) 589
GluI GCNGC 1 cut(s) 1381
GsaI CCCAGC 2 cut(s) 40, 1297
GsuI CTGGAG 2 cut(s) 656, 827
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 3 cut(s) 258, 498, 623
HapII CCGG 4 cut(s) 113, 259, 412, 1225
HhaI GCGC 1 cut(s) 590
Hin1II CATG 8 cut(s) 95, 182, 421, 552, 773, 1129, 1166, 1343
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HindIII AAGCTT 2 cut(s) 71, 692
HinfI GANTC 5 cut(s) 119, 284, 299, 659, 722
HpaI GTTAAC 1 cut(s) 513
HpaII CCGG 4 cut(s) 113, 259, 412, 1225
HphI GGTGA 1 cut(s) 844
Hpy166II GTNNAC 3 cut(s) 513, 849, 1168
Hpy188I TCNGA 6 cut(s) 304, 432, 652, 679, 730, 900
Hpy8I GTNNAC 3 cut(s) 513, 849, 1168
Hpy99I CGWCG 1 cut(s) 1317
HpyAV CCTTC 5 cut(s) 415, 509, 876, 956, 1383
HpyCH4III ACNGT 2 cut(s) 986, 1246
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 8 cut(s) 385, 1016, 1031, 1120, 1125, 1249, 1383, 1419
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 869
HpyF3I CTNAG 4 cut(s) 69, 132, 591, 899
HpySE526I ACGT 1 cut(s) 972
Hsp92II CATG 8 cut(s) 95, 182, 421, 552, 773, 1129, 1166, 1343
HspAI GCGC 1 cut(s) 588
KpnI GGTACC 1 cut(s) 1231
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 540
KspAI GTTAAC 1 cut(s) 513
Kzo9I GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
LmnI GCTCC 2 cut(s) 846, 1199
Lsp1109I GCAGC 1 cut(s) 1367
LweI GCATC 3 cut(s) 68, 975, 1444
MaeI CTAG 3 cut(s) 504, 789, 941
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 3 cut(s) 1105, 1276, 1358
MalI GATC 7 cut(s) 351, 429, 542, 654, 814, 1309, 1331
MboI GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
MboII GAAGA 5 cut(s) 359, 639, 700, 967, 1074
MflI RGATCY 1 cut(s) 1307
MhlI GDGCHC 1 cut(s) 188
MlyI GAGTC 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 1127
MroNI GCCGGC 1 cut(s) 258
MseI TTAA 4 cut(s) 407, 512, 708, 1395
MslI CAYNNNNRTG 1 cut(s) 774
MspA1I CMGCKG 1 cut(s) 1452
MspI CCGG 4 cut(s) 113, 259, 412, 1225
MspR9I CCNGG 2 cut(s) 113, 413
Mva1269I GAATGC 2 cut(s) 68, 1448
MvnI CGCG 1 cut(s) 1272
MwoI GCNNNNNNNGC 2 cut(s) 87, 869
NaeI GCCGGC 1 cut(s) 260
NciI CCSGG 2 cut(s) 113, 413
NdeII GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 8 cut(s) 95, 182, 421, 552, 773, 1129, 1166, 1343
NlaIV GGNNCC 4 cut(s) 263, 1170, 1201, 1229
NmuCI GTSAC 1 cut(s) 1276
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 1 cut(s) 1343
PagI TCATGA 3 cut(s) 178, 417, 769
PciI ACATGT 1 cut(s) 1339
PctI GAATGC 2 cut(s) 68, 1448
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 4 cut(s) 119, 284, 299, 659
Pfl23II CGTACG 1 cut(s) 1267
PflMI CCANNNNNTGG 1 cut(s) 879
PkrI GCNGC 1 cut(s) 1382
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
PscI ACATGT 1 cut(s) 1339
PshAI GACNNNNGTC 1 cut(s) 309
PshBI ATTAAT 1 cut(s) 1395
Psp1406I AACGTT 1 cut(s) 972
PspFI CCCAGC 2 cut(s) 36, 1293
PspLI CGTACG 1 cut(s) 1267
PspN4I GGNNCC 4 cut(s) 263, 1170, 1201, 1229
PspPI GGNCC 2 cut(s) 1137, 1168
PsrI GAACNNNNNNTAC 2 cut(s) 792, 824
PstI CTGCAG 1 cut(s) 387
PsuI RGATCY 1 cut(s) 1307
RsaI GTAC 8 cut(s) 246, 333, 641, 787, 810, 1182, 1229, 1269
RsaNI GTAC 8 cut(s) 245, 332, 640, 786, 809, 1181, 1228, 1268
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 4 cut(s) 407, 512, 708, 1395
SatI GCNGC 1 cut(s) 1381
Sau3AI GATC 7 cut(s) 349, 427, 540, 652, 812, 1307, 1329
Sau96I GGNCC 2 cut(s) 1137, 1168
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 2 cut(s) 113, 413
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 3 cut(s) 68, 975, 1444
SfcI CTRYAG 2 cut(s) 383, 625
SinI GGWCC 2 cut(s) 1137, 1168
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
SpeI ACTAGT 1 cut(s) 788
SsiI CCGC 3 cut(s) 612, 1140, 1450
SspMI CTAG 3 cut(s) 504, 789, 941
StyD4I CCNGG 2 cut(s) 111, 411
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 986, 1246
TaiI ACGT 1 cut(s) 975
TaqI TCGA 5 cut(s) 76, 193, 379, 468, 684
TatI WGTACW 4 cut(s) 244, 331, 639, 785
TfiI GAWTC 4 cut(s) 119, 284, 299, 659
Tru1I TTAA 4 cut(s) 407, 512, 708, 1395
Tru9I TTAA 4 cut(s) 407, 512, 708, 1395
TscAI CASTG 2 cut(s) 1180, 1251
TseFI GTSAC 1 cut(s) 1276
TseI GCWGC 1 cut(s) 1380
Tsp45I GTSAC 1 cut(s) 1276
TspDTI ATGAA 8 cut(s) 360, 374, 434, 651, 758, 1116, 1365, 1395
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 2 cut(s) 1180, 1251
Van91I CCANNNNNTGG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 1137, 1168
VspI ATTAAT 1 cut(s) 1395
XagI CCTNNNNNAGG 1 cut(s) 1207
XapI RAATTY 3 cut(s) 338, 375, 701
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 1 cut(s) 1343
XmaJI CCTAGG 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 3 cut(s) 504, 789, 941
ZrmI AGTACT 1 cut(s) 641
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.