Rh7CG297500

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0021482
Physical Location & Seq
Forward (+)
30523927 .. 30527722
3796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG297500.1

Sequence Viewer

Length: 1608 bp
ATGCAAGGCAAAATTTTACCAATTTTCTTTAATGTGGAAACCTCCATTTTACGAAAGAAAAATGGATTCGCAAGAACAGGCTTCGACATGCTTGATACAGAAGACATGGGAAAGCTGGAGTGGTGGACAACTGCTCCGAAAGAAGTGGCTGATATAGCTGGTTGGGTTTCAACTGACTGGGAGACCCAGCTTGTCAAAGAAATTGTAGCAGAATGCTCTAAGCTTCGATGCGATGTATGCCATGATTGGAAAAGTTTTATCCCGGCAGATTCAAGTCAAGCTGAGTATGTGGAACATCCTTTCTGGGGACAAAATTACTGCCCCTCTCATGAGGGCACTACTCGAAAGTGTTGTAGCTGTGAAAGATTAGAACCAAAGGACACAAAGTATTTTGTACTTGACGATGGCCGGCACCTATGTCCAGAGTGTCAAGAATCAGCAATTATGGATTCTGACCAATGTCAATCCCTTTACCTTGAAGTACAAAAATTCTATGAAGATCACGATATGAAATTGGGACACCAAATTCGACTGCAGTTGGTTGGCAGACAAAAATTAAACCGGGTCATGAAGGGAGATCAGAGTAATTGTGATGGAGAAATTAGAGCAGCTTGCCTCGAAGCCAAAGAAATACTCATCTGGTATGGCCTTCCTAGGTTGTTAACAGGGTCAATTCTGGCTCAAGAGATGATCAAAGCATGGCTAATGCTTCAAGTCTATCCACATTTCAAATTGAGCGCTGAGGTTGAAAGAGGTATCTGCGGAGTGCTGGCCTATAGTTGGCTGGAGTACTACTCTTCTGATCCTGATTCATCTAAAATATGGTCTGACTTCGAGAAGAAGCTTGTTGAATTTATTAAACACCAAATTGAGTCATCAGATTATGGAGAAGTATTCAGGAAAGCTAACAAAGTGGTTCATGAGTATGGTCTATGTACTAGTGCGATTGAACACATTGTACGATCAATGTATTTCAAAGTGGTGAAACAGCTCCAGTATACCAGCATTTCTGCCCCTTCAGCCAGTTCGTGGAAGTATGATGTGTTTCTGAGTTTTAGAGGGGAAACCCGAATGGGTTTTACAAGCCGTCTAGATAAAGCATTGAAGATGGAAGGCATCAACGTTTTCACGGACAGTCTAATCAAAAGAGGTGCGAGTATTCCTGCAACGCTCTTGACTGCAATCCTGAAATCAAGGTTTGCCATCATTGTCCTCTCGCAGAACTACACTTCTTCAATGTGGTGTTTAGATGAAGTTACAAAGGCTTTGCAATGCATGGACAAGAGGTCCGCAGTTTTCCCAATTTTTTACCATGTGGACCCCAGTGATGTACGATATGATAAGATGGGAGCCTATCAAAGGCATAGCACTCGCCGGTACCTAAAGAAAACAACAGTGCAAGACTGGACAATGAGCGTACGCGAAGTGACCAATGTTAGTGGCTGGGTTTCAAGTGGATCTCGTCAGGGGGAGGATCAGATCATCACAGATGTTGTAAATTTATTGCGTAACAGAGTGGTATGTGCTGGCTGCAACAGAAGGATTAATTATGAGACTTCATATTTGGGGTGCAACCATCGTGTTTGGCATCAAGAATGCTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

535

Amino Acids

61.77

Weight (kDa)

7.16

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 207 - 320 1.1e-26 Protein DA1
TIR PF01582 345 - 502 5.1e-33 TIR domain
TIR_2 PF13676 348 - 441 5e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021482)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0214001
rosa_samantha Rh7AG278600 Rh7CG297500 Rh7DG284100
rosa_wichuraiana Rw7G023960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1377
AccB1I GGYRCC 2 cut(s) 411, 1377
AccB7I CCANNNNNTGG 1 cut(s) 1029
AccI GTMKAC 1 cut(s) 998
AccII CGCG 1 cut(s) 1422
AciI CCGC 3 cut(s) 762, 1290, 1603
AclI AACGTT 1 cut(s) 1122
AclWI GGATC 3 cut(s) 797, 1465, 1482
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 5 cut(s) 12, 488, 525, 851, 1498
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 8 cut(s) 396, 483, 791, 937, 960, 1332, 1379, 1419
AfeI AGCGCT 1 cut(s) 739
AfiI CCNNNNNNNGG 4 cut(s) 305, 780, 1029, 1359
AhdI GACNNNNNGTC 1 cut(s) 1285
AhlI ACTAGT 1 cut(s) 938
Alw26I GTCTC 2 cut(s) 176, 1547
AlwI GGATC 3 cut(s) 797, 1465, 1482
Aor51HI AGCGCT 1 cut(s) 739
AoxI GGCC 3 cut(s) 406, 646, 771
ApeKI GCWGC 2 cut(s) 608, 1530
ApoI RAATTY 5 cut(s) 12, 488, 525, 851, 1498
ArsI GACNNNNNNTTYG 4 cut(s) 510, 542, 1546, 1578
AseI ATTAAT 1 cut(s) 1545
Asp718I GGTACC 1 cut(s) 1377
AspA2I CCTAGG 1 cut(s) 653
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 2 cut(s) 1287, 1318
AsuC2I CCSGG 2 cut(s) 263, 563
AsuHPI GGTGA 1 cut(s) 994
AvaII GGWCC 2 cut(s) 1287, 1318
AvrII CCTAGG 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 411, 1377
BbsI GAAGAC 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 620, 1517
BccI CCATC 6 cut(s) 398, 587, 1102, 1211, 1339, 1584
BceAI ACGGC 1 cut(s) 1071
BcgI CGANNNNNNTGC 2 cut(s) 1352, 1386
BclI TGATCA 1 cut(s) 690
BcnI CCSGG 2 cut(s) 263, 563
BcoDI GTCTC 2 cut(s) 176, 1547
BcuI ACTAGT 1 cut(s) 938
BfaI CTAG 3 cut(s) 654, 939, 1091
BfmI CTRYAG 2 cut(s) 533, 775
BfoI RGCGCY 1 cut(s) 741
BisI GCNGC 2 cut(s) 609, 1531
BlnI CCTAGG 1 cut(s) 653
BlsI GCNGC 2 cut(s) 610, 1532
BmcAI AGTACT 1 cut(s) 791
Bme1390I CCNGG 2 cut(s) 263, 563
Bme18I GGWCC 2 cut(s) 1287, 1318
BmeRI GACNNNNNGTC 1 cut(s) 1285
BmgT120I GGNCC 2 cut(s) 1287, 1318
BmiI GGNNCC 4 cut(s) 413, 1320, 1351, 1379
BmrFI CCNGG 2 cut(s) 263, 563
BmrI ACTGGG 2 cut(s) 187, 1317
BmsI GCATC 3 cut(s) 218, 1125, 1597
BmuI ACTGGG 2 cut(s) 187, 1317
BoxI GACNNNNGTC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 779, 811
BpmI CTGGAG 3 cut(s) 137, 806, 977
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 666
BpuMI CCSGG 2 cut(s) 263, 563
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 653
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 4 cut(s) 305, 780, 1029, 1359
Bse118I RCCGGY 2 cut(s) 408, 1374
Bse1I ACTGG 5 cut(s) 182, 994, 1023, 1323, 1409
Bse3DI GCAATG 1 cut(s) 1277
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 4 cut(s) 305, 780, 1029, 1359
BseMI GCAATG 1 cut(s) 1277
BseMII CTCAG 3 cut(s) 273, 732, 1040
BseNI ACTGG 5 cut(s) 182, 994, 1023, 1323, 1409
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 2 cut(s) 620, 1517
BseYI CCCAGC 2 cut(s) 186, 1443
Bsh1236I CGCG 1 cut(s) 1422
BshFI GGCC 3 cut(s) 408, 648, 773
BshNI GGYRCC 2 cut(s) 411, 1377
BsiSI CCGG 4 cut(s) 263, 409, 562, 1375
BsiWI CGTACG 1 cut(s) 1417
BslFI GGGAC 2 cut(s) 321, 531
BslI CCNNNNNNNGG 4 cut(s) 305, 780, 1029, 1359
BsmAI GTCTC 2 cut(s) 176, 1547
BsmFI GGGAC 2 cut(s) 321, 531
BsmI GAATGC 2 cut(s) 218, 1601
BsnI GGCC 3 cut(s) 408, 648, 773
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
BspACI CCGC 3 cut(s) 762, 1290, 1603
BspANI GGCC 3 cut(s) 408, 648, 773
BspCNI CTCAG 3 cut(s) 274, 733, 1041
BspFNI CGCG 1 cut(s) 1422
BspHI TCATGA 3 cut(s) 328, 567, 919
BspLI GGNNCC 4 cut(s) 413, 1320, 1351, 1379
BspMAI CTGCAG 1 cut(s) 537
BspPI GGATC 3 cut(s) 797, 1465, 1482
BspT107I GGYRCC 2 cut(s) 411, 1377
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 1277
BsrFI RCCGGY 2 cut(s) 408, 1374
BsrI ACTGG 5 cut(s) 182, 994, 1023, 1323, 1409
BssAI RCCGGY 2 cut(s) 408, 1374
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
BssNAI GTATAC 1 cut(s) 999
BssT1I CCWWGG 1 cut(s) 653
Bst1107I GTATAC 1 cut(s) 999
Bst4CI ACNGT 2 cut(s) 1136, 1396
Bst6I CTCTTC 1 cut(s) 802
BstC8I GCNNGC 4 cut(s) 410, 613, 771, 1528
BstDEI CTNAG 4 cut(s) 219, 282, 741, 1049
BstENI CCTNNNNNAGG 1 cut(s) 1357
BstF5I GGATG 1 cut(s) 295
BstFNI CGCG 1 cut(s) 1422
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 8 cut(s) 502, 580, 693, 805, 965, 1460, 1477, 1482
BstMAI GTCTC 2 cut(s) 176, 1547
BstMBI GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
BstMWI GCNNNNNNNGC 3 cut(s) 155, 237, 1019
BstNSI RCATGY 1 cut(s) 91
BstPAI GACNNNNGTC 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 261, 561
BstSFI CTRYAG 2 cut(s) 533, 775
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 1 cut(s) 1422
BstV1I GCAGC 2 cut(s) 620, 1517
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 1457
BstYI RGATCY 1 cut(s) 1457
BstZ17I GTATAC 1 cut(s) 999
BsuRI GGCC 3 cut(s) 408, 648, 773
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 1330, 1401
Cac8I GCNNGC 4 cut(s) 410, 613, 771, 1528
CciI TCATGA 3 cut(s) 328, 567, 919
CfoI GCGC 1 cut(s) 740
Cfr10I RCCGGY 2 cut(s) 408, 1374
Cfr13I GGNCC 2 cut(s) 1287, 1318
Csp6I GTAC 8 cut(s) 395, 482, 790, 936, 959, 1331, 1378, 1418
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviAII CATG 9 cut(s) 88, 106, 242, 329, 568, 699, 920, 1276, 1313
CviQI GTAC 8 cut(s) 395, 482, 790, 936, 959, 1331, 1378, 1418
DdeI CTNAG 4 cut(s) 219, 282, 741, 1049
DpnI GATC 8 cut(s) 501, 579, 692, 804, 964, 1459, 1476, 1481
DpnII GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
DriI GACNNNNNGTC 1 cut(s) 1285
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 802
Eam1105I GACNNNNNGTC 1 cut(s) 1285
EarI CTCTTC 1 cut(s) 802
Eco130I CCWWGG 1 cut(s) 653
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 1287, 1318
Eco47III AGCGCT 1 cut(s) 739
Eco57I CTGAAG 1 cut(s) 1002
EcoNI CCTNNNNNAGG 1 cut(s) 1357
EcoT14I CCWWGG 1 cut(s) 653
EcoT22I ATGCAT 1 cut(s) 1277
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 9 cut(s) 91, 109, 245, 332, 571, 702, 923, 1279, 1316
FaqI GGGAC 2 cut(s) 321, 531
FatI CATG 9 cut(s) 87, 105, 241, 328, 567, 698, 919, 1275, 1312
FbaI TGATCA 1 cut(s) 690
FblI GTMKAC 1 cut(s) 998
Fnu4HI GCNGC 2 cut(s) 609, 1531
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 609, 1531
FspBI CTAG 3 cut(s) 654, 939, 1091
GlaI GCGC 1 cut(s) 739
GluI GCNGC 2 cut(s) 609, 1531
GsaI CCCAGC 2 cut(s) 190, 1447
GsuI CTGGAG 3 cut(s) 137, 806, 977
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 3 cut(s) 408, 648, 773
HapII CCGG 4 cut(s) 263, 409, 562, 1375
HhaI GCGC 1 cut(s) 740
Hin1II CATG 9 cut(s) 91, 109, 245, 332, 571, 702, 923, 1279, 1316
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 2 cut(s) 221, 842
HinfI GANTC 6 cut(s) 66, 269, 434, 449, 809, 872
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 4 cut(s) 263, 409, 562, 1375
HphI GGTGA 1 cut(s) 994
Hpy166II GTNNAC 4 cut(s) 126, 663, 999, 1318
Hpy188I TCNGA 8 cut(s) 138, 454, 582, 802, 829, 880, 1050, 1479
Hpy8I GTNNAC 4 cut(s) 126, 663, 999, 1318
HpyAV CCTTC 5 cut(s) 565, 659, 1026, 1106, 1533
HpyCH4III ACNGT 2 cut(s) 1136, 1396
HpyCH4IV ACGT 1 cut(s) 1122
HpyCH4V TGCA 9 cut(s) 4, 535, 1166, 1181, 1270, 1275, 1399, 1533, 1572
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 237, 1019
HpyF3I CTNAG 4 cut(s) 219, 282, 741, 1049
HpySE526I ACGT 1 cut(s) 1122
Hsp92II CATG 9 cut(s) 91, 109, 245, 332, 571, 702, 923, 1279, 1316
HspAI GCGC 1 cut(s) 738
KpnI GGTACC 1 cut(s) 1381
KroI GCCGGC 1 cut(s) 408
KroNI GCCGGC 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 690
KspAI GTTAAC 1 cut(s) 663
Kzo9I GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
LmnI GCTCC 3 cut(s) 139, 996, 1349
Lsp1109I GCAGC 2 cut(s) 620, 1517
LweI GCATC 3 cut(s) 218, 1125, 1597
MaeI CTAG 3 cut(s) 654, 939, 1091
MaeII ACGT 1 cut(s) 1122
MaeIII GTNAC 3 cut(s) 1255, 1426, 1508
MalI GATC 8 cut(s) 501, 579, 692, 804, 964, 1459, 1476, 1481
MboI GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
MboII GAAGA 6 cut(s) 113, 509, 789, 850, 1117, 1224
MflI RGATCY 1 cut(s) 1457
MhlI GDGCHC 1 cut(s) 338
MlyI GAGTC 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 1277
MroNI GCCGGC 1 cut(s) 408
MseI TTAA 5 cut(s) 30, 557, 662, 858, 1545
MslI CAYNNNNRTG 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 1605
MspI CCGG 4 cut(s) 263, 409, 562, 1375
MspR9I CCNGG 2 cut(s) 263, 563
Mva1269I GAATGC 2 cut(s) 218, 1601
MvnI CGCG 1 cut(s) 1422
MwoI GCNNNNNNNGC 3 cut(s) 155, 237, 1019
NaeI GCCGGC 1 cut(s) 410
NciI CCSGG 2 cut(s) 263, 563
NdeII GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
NgoMIV GCCGGC 1 cut(s) 408
NlaIII CATG 9 cut(s) 91, 109, 245, 332, 571, 702, 923, 1279, 1316
NlaIV GGNNCC 4 cut(s) 413, 1320, 1351, 1379
NmuCI GTSAC 1 cut(s) 1426
NsiI ATGCAT 1 cut(s) 1277
NspI RCATGY 1 cut(s) 91
PagI TCATGA 3 cut(s) 328, 567, 919
PctI GAATGC 2 cut(s) 218, 1601
PdiI GCCGGC 1 cut(s) 410
PfeI GAWTC 5 cut(s) 66, 269, 434, 449, 809
Pfl23II CGTACG 1 cut(s) 1417
PflMI CCANNNNNTGG 1 cut(s) 1029
PkrI GCNGC 2 cut(s) 610, 1532
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
PshAI GACNNNNGTC 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 1545
Psp1406I AACGTT 1 cut(s) 1122
PspFI CCCAGC 2 cut(s) 186, 1443
PspLI CGTACG 1 cut(s) 1417
PspN4I GGNNCC 4 cut(s) 413, 1320, 1351, 1379
PspPI GGNCC 2 cut(s) 1287, 1318
PsrI GAACNNNNNNTAC 2 cut(s) 942, 974
PstI CTGCAG 1 cut(s) 537
PsuI RGATCY 1 cut(s) 1457
RsaI GTAC 8 cut(s) 396, 483, 791, 937, 960, 1332, 1379, 1419
RsaNI GTAC 8 cut(s) 395, 482, 790, 936, 959, 1331, 1378, 1418
RseI CAYNNNNRTG 1 cut(s) 924
SaqAI TTAA 5 cut(s) 30, 557, 662, 858, 1545
SatI GCNGC 2 cut(s) 609, 1531
Sau3AI GATC 8 cut(s) 499, 577, 690, 802, 962, 1457, 1474, 1479
Sau96I GGNCC 2 cut(s) 1287, 1318
ScaI AGTACT 1 cut(s) 791
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 2 cut(s) 263, 563
SduI GDGCHC 1 cut(s) 338
SfaNI GCATC 3 cut(s) 218, 1125, 1597
SfcI CTRYAG 2 cut(s) 533, 775
SinI GGWCC 2 cut(s) 1287, 1318
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
SpeI ACTAGT 1 cut(s) 938
SsiI CCGC 3 cut(s) 762, 1290, 1603
SspMI CTAG 3 cut(s) 654, 939, 1091
StyD4I CCNGG 2 cut(s) 261, 561
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 2 cut(s) 1136, 1396
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 6 cut(s) 84, 226, 343, 529, 618, 834
TatI WGTACW 4 cut(s) 394, 481, 789, 935
TfiI GAWTC 5 cut(s) 66, 269, 434, 449, 809
Tru1I TTAA 5 cut(s) 30, 557, 662, 858, 1545
Tru9I TTAA 5 cut(s) 30, 557, 662, 858, 1545
TscAI CASTG 2 cut(s) 1330, 1401
TseFI GTSAC 1 cut(s) 1426
TseI GCWGC 2 cut(s) 608, 1530
Tsp45I GTSAC 1 cut(s) 1426
TspDTI ATGAA 7 cut(s) 510, 524, 584, 801, 908, 1266, 1548
TspGWI ACGGA 1 cut(s) 1145
TspRI CASTG 2 cut(s) 1330, 1401
Van91I CCANNNNNTGG 1 cut(s) 1029
VpaK11BI GGWCC 2 cut(s) 1287, 1318
VspI ATTAAT 1 cut(s) 1545
XagI CCTNNNNNAGG 1 cut(s) 1357
XapI RAATTY 5 cut(s) 12, 488, 525, 851, 1498
XbaI TCTAGA 1 cut(s) 1090
XceI RCATGY 1 cut(s) 91
XmaJI CCTAGG 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 998
XspI CTAG 3 cut(s) 654, 939, 1091
ZrmI AGTACT 1 cut(s) 791
Zsp2I ATGCAT 1 cut(s) 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.