Rw7G023960

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0021482
Physical Location & Seq
Forward (+)
29051297 .. 29052589
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023960.1

Sequence Viewer

Length: 552 bp
ATGAAGGGAGATCAGAGTAATTGTGATGGAGAAATTAGAGCAGTCTGCCTCGAAGCCAAAGAAATACTCATCTGGTATGGCCTTCCTAGGTTGTTGACAGGGTCAATTCTGGCTCAAGAGATGATCAAAGCATGGCTAATGCTTCAAGTCTATCCACATTTCAAATTGAGCGCTGAGGTTGAAAGATGTATCTGCGGAGTGCTGGCCTATAGTTGGCTGGAGTACTACTCTTCTGATCCTGATTCATCTAAAATATGGTCTGACTTCGAGAAGAAGCTTGTTGAATTTATTAAACACCAAATTGAGTCATCATATTATGGAGAAGCATTCAGGAAAGCTAACAAAGTGGTTCATGAGTACGGTCTATGTACTAGTGCGATTAAATACATTGTACGATCAATGCATTTCAAAGTGGAGGATGAGATCATCACAGATGTTGTAAATGAATTGCGTAACAGAGTGGTATGTGCTGGCTGCAACAGAAGGATTAATTATGAAACTTCATATTTGAGGTGCAACCATCGTGTTTGGCATCAAGAATGCTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

183

Amino Acids

21.51

Weight (kDa)

6.98

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 18 - 123 1.3e-25 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021482)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0214001
rosa_samantha Rh7AG278600 Rh7CG297500 Rh7DG284100
rosa_wichuraiana Rw7G023960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 195, 547
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 1 cut(s) 284
AfaI GTAC 4 cut(s) 224, 359, 370, 393
AfeI AGCGCT 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 5 cut(s) 146, 163, 182, 284, 409
AhlI ACTAGT 1 cut(s) 371
AluBI AGCT 2 cut(s) 277, 338
AluI AGCT 2 cut(s) 277, 338
AlwI GGATC 1 cut(s) 230
Aor51HI AGCGCT 1 cut(s) 172
AoxI GGCC 2 cut(s) 79, 204
ApeKI GCWGC 1 cut(s) 474
ApoI RAATTY 1 cut(s) 284
AseI ATTAAT 1 cut(s) 489
AspA2I CCTAGG 1 cut(s) 86
AspLEI GCGC 1 cut(s) 173
AvrII CCTAGG 1 cut(s) 86
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 461
BccI CCATC 2 cut(s) 20, 528
BclI TGATCA 1 cut(s) 123
BcuI ACTAGT 1 cut(s) 371
BfaI CTAG 2 cut(s) 87, 372
BfmI CTRYAG 1 cut(s) 208
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 1 cut(s) 475
BlnI CCTAGG 1 cut(s) 86
BlsI GCNGC 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 224
BmsI GCATC 1 cut(s) 541
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 86
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 165
BseXI GCAGC 1 cut(s) 461
BshFI GGCC 2 cut(s) 81, 206
BslI CCNNNNNNNGG 1 cut(s) 213
BsmI GAATGC 2 cut(s) 326, 545
BsnI GGCC 2 cut(s) 81, 206
Bsp143I GATC 5 cut(s) 10, 123, 235, 395, 423
BspACI CCGC 2 cut(s) 195, 547
BspANI GGCC 2 cut(s) 81, 206
BspCNI CTCAG 1 cut(s) 166
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 5 cut(s) 10, 123, 235, 395, 423
BssT1I CCWWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 204, 472
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 5 cut(s) 13, 126, 238, 398, 426
BstMBI GATC 5 cut(s) 10, 123, 235, 395, 423
BstSFI CTRYAG 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 81, 206
BtsCI GGATG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 204, 472
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 173
Csp6I GTAC 4 cut(s) 223, 358, 369, 392
CviAII CATG 2 cut(s) 132, 353
CviJI RGCY 9 cut(s) 56, 81, 113, 136, 206, 217, 277, 338, 474
CviKI_1 RGCY 9 cut(s) 56, 81, 113, 136, 206, 217, 277, 338, 474
CviQI GTAC 4 cut(s) 223, 358, 369, 392
DdeI CTNAG 1 cut(s) 174
DpnI GATC 5 cut(s) 12, 125, 237, 397, 425
DpnII GATC 5 cut(s) 10, 123, 235, 395, 423
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 86
Eco47III AGCGCT 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 86
FaeI CATG 2 cut(s) 135, 356
FatI CATG 2 cut(s) 131, 352
FbaI TGATCA 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 475
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 1 cut(s) 475
FspBI CTAG 2 cut(s) 87, 372
GlaI GCGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 2 cut(s) 81, 206
HhaI GCGC 1 cut(s) 173
Hin1II CATG 2 cut(s) 135, 356
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 2 cut(s) 242, 305
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 3 cut(s) 15, 235, 262
Hpy188III TCNNGA 6 cut(s) 116, 239, 268, 331, 353, 536
Hpy8I GTNNAC 1 cut(s) 96
HpyAV CCTTC 2 cut(s) 92, 477
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 403, 477, 516
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 2 cut(s) 135, 356
HspAI GCGC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 5 cut(s) 10, 123, 235, 395, 423
LpnPI CCDG 8 cut(s) 58, 84, 95, 188, 203, 252, 316, 456
Lsp1109I GCAGC 1 cut(s) 461
LweI GCATC 1 cut(s) 541
MaeI CTAG 2 cut(s) 87, 372
MaeIII GTNAC 1 cut(s) 452
MalI GATC 5 cut(s) 12, 125, 237, 397, 425
MboI GATC 5 cut(s) 10, 123, 235, 395, 423
MboII GAAGA 2 cut(s) 222, 283
MluCI AATT 8 cut(s) 19, 33, 105, 164, 284, 300, 446, 490
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 4 cut(s) 59, 169, 409, 504
Mph1103I ATGCAT 1 cut(s) 405
MseI TTAA 3 cut(s) 291, 381, 489
MspA1I CMGCKG 1 cut(s) 549
Mva1269I GAATGC 2 cut(s) 326, 545
NdeII GATC 5 cut(s) 10, 123, 235, 395, 423
NlaIII CATG 2 cut(s) 135, 356
NsiI ATGCAT 1 cut(s) 405
PagI TCATGA 1 cut(s) 352
PctI GAATGC 2 cut(s) 326, 545
PfeI GAWTC 1 cut(s) 242
PflFI GACNNNGTC 1 cut(s) 100
PkrI GCNGC 1 cut(s) 476
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 489
PsyI GACNNNGTC 1 cut(s) 100
RsaI GTAC 4 cut(s) 224, 359, 370, 393
RsaNI GTAC 4 cut(s) 223, 358, 369, 392
SaqAI TTAA 3 cut(s) 291, 381, 489
SatI GCNGC 1 cut(s) 475
Sau3AI GATC 5 cut(s) 10, 123, 235, 395, 423
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 314
SetI ASST 5 cut(s) 92, 180, 279, 340, 515
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
SpeI ACTAGT 1 cut(s) 371
Sse9I AATT 8 cut(s) 19, 33, 105, 164, 284, 300, 446, 490
SsiI CCGC 2 cut(s) 195, 547
SspMI CTAG 2 cut(s) 87, 372
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 362
TaqI TCGA 2 cut(s) 51, 267
TasI AATT 8 cut(s) 19, 33, 105, 164, 284, 300, 446, 490
TatI WGTACW 2 cut(s) 222, 368
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 3 cut(s) 291, 381, 489
Tru9I TTAA 3 cut(s) 291, 381, 489
TseI GCWGC 1 cut(s) 474
TspDTI ATGAA 6 cut(s) 17, 234, 341, 459, 492, 510
Tth111I GACNNNGTC 1 cut(s) 100
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 284
XmaJI CCTAGG 1 cut(s) 86
XspI CTAG 2 cut(s) 87, 372
ZrmI AGTACT 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.