RLG00000000169

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0013910
Physical Location & Seq
Forward (+)
776568 .. 778257
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000169

Sequence Viewer

Length: 1506 bp
ATGATTATTCGTTCGCCGGAACCAGAAGTTAAAATTTTGGTAGATAGGGATCCCGTAAAAACTTCTTTCGAGGAATGGGCCAGACCCGGTCATTTCTCAAGAACAATAGCTAAGGGGCCTGATACTACCACTTGGATCTGGAACCTACACGCTGATGCTCACGATTTCGATAGCCATACCAGTGATTTGGAGGAGATCTCTCGAAAAGTATTTAGTGCCCATTTTGGTCAACTCTCCATCATCTTTCTTTGGCTGAGTGGTATGTATTTCCACGGTGCTCGTTTTTCCAATTATGAAGCATGGCTAAGTGATCCTACTCACATTGGACCTAGTGCCCAGGTGGTTTGGCCAATAGTGGGTCAAGAAATATTGAATGGTGATGTAGGCGGGGGTTTCCGAGGAATACAAATAACCTCTGGGTTTTTTCAGCTTTGGCGAGCATCTGGAATAACTAATGAATTACAACTCTATTGTACTGCAATTGGTGCATTGGTCTTTGCTGCCTTAATGCTTTTTGCGGGTTGGTTCCATTATCACAAAGCTGCTCCGAAATTGGCTTGGTTCCAAGATGTAGAATCTATGTTGAATCACCATTTAGCAGGGCTATTAGGCCTTGGGTCTCTTGGTTGGGCGGGGCATCAAGTACACGTATCGTTACCAATTAACCAATTTCTAAACGCTGGAGTAGATCCTAAAGAGATTCCACTTCCTCATGAATTTATCTTGAATCGGGATCTTTTGGCTCAACTTTATCCCAGTTTTGCTGAGGGAGCGACCCCATTTTTCACCTTGAATTGGTCAAAATATGCGGAATTTCTTACTTTTCGTGGAGGATTAGATCCAGTAACTGGAGGTCTATGGCTGACCGATATTGCACACCATCATTTAGCTATTGCAATTCTTTTCCTGGTAGCGGGTCACATGTATAAGACCAATTGGGGCATTGGTCATGGTATAAAAGATATTTTAGAGGCTCATAAAGGCCCATTTACCGGCCAGGGCCATAAAGGCCTATATGAGATCCTAACAACGTCATGGCATGCTCAATTATCTCTTAACCTAGCTATGTTAGGATCTTTAACCATTATTGTAGCTCACCATATGTATGCGATGCCCCCTTATCCATATCTAGCTACTGACTATGGTACACAACTTTCATTGTTCACACATCATATGTGGATTGGTGGATTTCTCATAGTTGGTGCTGCTGCGCATGCAGCCATTTTTATGGTAAGAGACTATGATCCAACTACTCGATACAACGATCTATTAGATCGTGTCCTTAGGCATCGCGATGCAATCATATCACATCTCAACTGGCCCGTCTTTGCTCAATGGATACAAAACACCCACGCTTTAGCACCCGGCGCAACGGCCCCTGGTGCAACAACAAGCACCAGTTTGACTTGGGGGGGTGGTGATTTAGTGGCAGTGGGCGGTAAAGTTGCTTTGTTACCTATTCCATTAGGAACCGCAGATTTTTTGTTCGCGTTTGATACCCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaA_PsaB

Protein Analysis

502

Amino Acids

55.48

Weight (kDa)

6.23

Isoelectric Point (pI)

26.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaA_PsaB PF00223 32 - 440 3.9e-195 Photosystem I psaA/psaB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1212
AccB7I CCANNNNNTGG 1 cut(s) 848
AccII CGCG 2 cut(s) 1293, 1490
AciI CCGC 7 cut(s) 387, 518, 632, 809, 914, 1437, 1473
AcoI YGGCCR 2 cut(s) 347, 994
AcsI RAATTY 3 cut(s) 33, 716, 812
AfaI GTAC 3 cut(s) 475, 645, 1147
AfiI CCNNNNNNNGG 5 cut(s) 356, 795, 848, 913, 992
AflIII ACRYGT 2 cut(s) 646, 921
AgsI TTSAA 4 cut(s) 373, 586, 727, 793
AjnI CCWGG 4 cut(s) 336, 906, 996, 1378
AluBI AGCT 7 cut(s) 110, 430, 542, 890, 1064, 1094, 1133
AluI AGCT 7 cut(s) 110, 430, 542, 890, 1064, 1094, 1133
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 624, 1230
AlwNI CAGNNNCTG 1 cut(s) 848
ApeKI GCWGC 5 cut(s) 500, 542, 1205, 1208, 1217
ApoI RAATTY 3 cut(s) 33, 716, 812
AspLEI GCGC 2 cut(s) 1213, 1370
AspS9I GGNCC 7 cut(s) 78, 116, 326, 983, 1000, 1320, 1375
AsuC2I CCSGG 2 cut(s) 87, 1365
AsuHPI GGTGA 5 cut(s) 389, 581, 778, 1088, 1430
AvaII GGWCC 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 1283
BaeGI GKGCMC 2 cut(s) 220, 337
BalI TGGCCA 1 cut(s) 349
BamHI GGATCC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 5 cut(s) 487, 529, 1192, 1195, 1229
BccI CCATC 2 cut(s) 245, 888
BceAI ACGGC 1 cut(s) 1389
BciT130I CCWGG 4 cut(s) 338, 908, 998, 1380
BciVI GTATCC 1 cut(s) 1332
BcnI CCSGG 2 cut(s) 87, 1365
BcoDI GTCTC 2 cut(s) 624, 1230
BfaI CTAG 3 cut(s) 330, 1061, 1130
BfuI GTATCC 1 cut(s) 1332
BglI GCCNNNNNGGC 1 cut(s) 1008
BglII AGATCT 1 cut(s) 195
BisI GCNGC 5 cut(s) 501, 543, 1206, 1209, 1218
BlsI GCNGC 5 cut(s) 502, 544, 1207, 1210, 1219
Bme1390I CCNGG 6 cut(s) 87, 338, 908, 998, 1365, 1380
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 7 cut(s) 78, 116, 326, 983, 1000, 1320, 1375
BmiI GGNNCC 8 cut(s) 21, 51, 117, 143, 527, 563, 1377, 1471
BmrFI CCNGG 6 cut(s) 87, 338, 908, 998, 1365, 1380
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 6 cut(s) 145, 449, 646, 1101, 1285, 1297
BmuI ACTGGG 1 cut(s) 750
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 2 cut(s) 702, 870
Bpu10I CCTNAGC 2 cut(s) 111, 765
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 2 cut(s) 87, 1365
BsaAI YACGTR 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 624
BsaJI CCNNGG 6 cut(s) 271, 336, 397, 613, 997, 1378
Bsc4I CCNNNNNNNGG 5 cut(s) 356, 795, 848, 913, 992
Bse118I RCCGGY 1 cut(s) 992
Bse1I ACTGG 6 cut(s) 180, 756, 842, 853, 1322, 1398
Bse21I CCTNAGG 1 cut(s) 1283
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 4 cut(s) 338, 908, 998, 1380
BseDI CCNNGG 6 cut(s) 271, 336, 397, 613, 997, 1378
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 5 cut(s) 356, 795, 848, 913, 992
BseMII CTCAG 2 cut(s) 245, 756
BseNI ACTGG 6 cut(s) 180, 756, 842, 853, 1322, 1398
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 2 cut(s) 220, 337
BseXI GCAGC 5 cut(s) 487, 529, 1192, 1195, 1229
Bsh1236I CGCG 2 cut(s) 1293, 1490
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 4 cut(s) 17, 87, 993, 1365
BslI CCNNNNNNNGG 5 cut(s) 356, 795, 848, 913, 992
BsmAI GTCTC 2 cut(s) 624, 1230
Bso31I GGTCTC 1 cut(s) 624
Bsp1286I GDGCHC 3 cut(s) 220, 280, 337
Bsp68I TCGCGA 1 cut(s) 1293
BspACI CCGC 7 cut(s) 387, 518, 632, 809, 914, 1437, 1473
BspCNI CTCAG 2 cut(s) 246, 757
BspFNI CGCG 2 cut(s) 1293, 1490
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 8 cut(s) 21, 51, 117, 143, 527, 563, 1377, 1471
BspTNI GGTCTC 1 cut(s) 624
BsrFI RCCGGY 1 cut(s) 992
BsrI ACTGG 6 cut(s) 180, 756, 842, 853, 1322, 1398
BssAI RCCGGY 1 cut(s) 992
BssECI CCNNGG 6 cut(s) 271, 336, 397, 613, 997, 1378
BssT1I CCWWGG 1 cut(s) 613
Bst2UI CCWGG 4 cut(s) 338, 908, 998, 1380
Bst4CI ACNGT 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 3 cut(s) 438, 1041, 1215
BstDEI CTNAG 5 cut(s) 111, 254, 305, 765, 1283
BstDSI CCRYGG 1 cut(s) 271
BstFNI CGCG 2 cut(s) 1293, 1490
BstHHI GCGC 2 cut(s) 1213, 1370
BstMAI GTCTC 2 cut(s) 624, 1230
BstMWI GCNNNNNNNGC 7 cut(s) 485, 770, 1008, 1214, 1217, 1367, 1382
BstNI CCWGG 4 cut(s) 338, 908, 998, 1380
BstNSI RCATGY 3 cut(s) 925, 1043, 1217
BstSCI CCNGG 6 cut(s) 85, 336, 906, 996, 1363, 1378
BstSLI GKGCMC 2 cut(s) 220, 337
BstUI CGCG 2 cut(s) 1293, 1490
BstV1I GCAGC 5 cut(s) 487, 529, 1192, 1195, 1229
BstX2I RGATCY 8 cut(s) 49, 135, 195, 688, 733, 838, 1020, 1073
BstXI CCANNNNNNTGG 2 cut(s) 187, 1228
BstYI RGATCY 8 cut(s) 49, 135, 195, 688, 733, 838, 1020, 1073
Bsu36I CCTNAGG 1 cut(s) 1283
BsuI GTATCC 1 cut(s) 1332
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 3 cut(s) 1124, 1274, 1308
BtsI GCAGTG 1 cut(s) 1437
BtsIMutI CAGTG 2 cut(s) 187, 1437
BtuMI TCGCGA 1 cut(s) 1293
Cac8I GCNNGC 3 cut(s) 438, 1041, 1215
CaiI CAGNNNCTG 1 cut(s) 848
CciI TCATGA 1 cut(s) 712
CfoI GCGC 2 cut(s) 1213, 1370
Cfr10I RCCGGY 1 cut(s) 992
Cfr13I GGNCC 7 cut(s) 78, 116, 326, 983, 1000, 1320, 1375
Csp6I GTAC 3 cut(s) 474, 644, 1146
CviAII CATG 7 cut(s) 300, 713, 922, 950, 1035, 1040, 1214
CviQI GTAC 3 cut(s) 474, 644, 1146
DdeI CTNAG 5 cut(s) 111, 254, 305, 765, 1283
EaeI YGGCCR 2 cut(s) 347, 994
Eco130I CCWWGG 1 cut(s) 613
Eco147I AGGCCT 2 cut(s) 612, 1011
Eco31I GGTCTC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 1283
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 4 cut(s) 336, 906, 996, 1378
EcoT14I CCWWGG 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 7 cut(s) 303, 716, 925, 953, 1038, 1043, 1217
FatI CATG 7 cut(s) 299, 712, 921, 949, 1034, 1039, 1213
FauI CCCGC 4 cut(s) 380, 511, 625, 907
FauNDI CATATG 2 cut(s) 1101, 1173
Fnu4HI GCNGC 5 cut(s) 501, 543, 1206, 1209, 1218
Fsp4HI GCNGC 5 cut(s) 501, 543, 1206, 1209, 1218
FspBI CTAG 3 cut(s) 330, 1061, 1130
FspI TGCGCA 1 cut(s) 1212
GlaI GCGC 2 cut(s) 1212, 1369
GluI GCNGC 5 cut(s) 501, 543, 1206, 1209, 1218
GsuI CTGGAG 2 cut(s) 702, 870
HapII CCGG 4 cut(s) 17, 87, 993, 1365
HhaI GCGC 2 cut(s) 1213, 1370
Hin1II CATG 7 cut(s) 303, 716, 925, 953, 1038, 1043, 1217
Hin6I GCGC 2 cut(s) 1211, 1368
HinP1I GCGC 2 cut(s) 1211, 1368
HincII GTYRAC 1 cut(s) 230
HindII GTYRAC 1 cut(s) 230
HinfI GANTC 4 cut(s) 575, 586, 700, 727
HpaII CCGG 4 cut(s) 17, 87, 993, 1365
HphI GGTGA 5 cut(s) 389, 581, 778, 1088, 1430
Hpy166II GTNNAC 4 cut(s) 230, 646, 1148, 1164
Hpy188I TCNGA 2 cut(s) 398, 549
Hpy8I GTNNAC 4 cut(s) 230, 646, 1148, 1164
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 2 cut(s) 648, 1031
HpyCH4V TGCA 7 cut(s) 479, 488, 875, 896, 1217, 1298, 1385
HpyF10VI GCNNNNNNNGC 7 cut(s) 485, 770, 1008, 1214, 1217, 1367, 1382
HpyF3I CTNAG 5 cut(s) 111, 254, 305, 765, 1283
HpySE526I ACGT 2 cut(s) 648, 1031
Hsp92II CATG 7 cut(s) 303, 716, 925, 953, 1038, 1043, 1217
HspAI GCGC 2 cut(s) 1211, 1368
LmnI GCTCC 2 cut(s) 550, 770
Lsp1109I GCAGC 5 cut(s) 487, 529, 1192, 1195, 1229
LweI GCATC 6 cut(s) 145, 449, 646, 1101, 1285, 1297
MaeI CTAG 3 cut(s) 330, 1061, 1130
MaeII ACGT 2 cut(s) 648, 1031
MaeIII GTNAC 4 cut(s) 654, 844, 917, 1452
MfeI CAATTG 2 cut(s) 480, 934
MflI RGATCY 8 cut(s) 49, 135, 195, 688, 733, 838, 1020, 1073
MhlI GDGCHC 3 cut(s) 220, 280, 337
MlsI TGGCCA 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 1271
MnlI CCTC 9 cut(s) 64, 184, 392, 424, 720, 760, 824, 845, 964
Mox20I TGGCCA 1 cut(s) 349
MscI TGGCCA 1 cut(s) 349
MseI TTAA 5 cut(s) 30, 506, 663, 1056, 1079
MslI CAYNNNNRTG 5 cut(s) 153, 180, 1104, 1226, 1293
Msp20I TGGCCA 1 cut(s) 349
MspI CCGG 4 cut(s) 17, 87, 993, 1365
MspR9I CCNGG 6 cut(s) 87, 338, 908, 998, 1365, 1380
MunI CAATTG 2 cut(s) 480, 934
MvaI CCWGG 4 cut(s) 338, 908, 998, 1380
MvnI CGCG 2 cut(s) 1293, 1490
MwoI GCNNNNNNNGC 7 cut(s) 485, 770, 1008, 1214, 1217, 1367, 1382
NciI CCSGG 2 cut(s) 87, 1365
NdeI CATATG 2 cut(s) 1101, 1173
NlaIII CATG 7 cut(s) 303, 716, 925, 953, 1038, 1043, 1217
NlaIV GGNNCC 8 cut(s) 21, 51, 117, 143, 527, 563, 1377, 1471
NmuCI GTSAC 1 cut(s) 917
NruI TCGCGA 1 cut(s) 1293
NsbI TGCGCA 1 cut(s) 1212
NspI RCATGY 3 cut(s) 925, 1043, 1217
PaeI GCATGC 2 cut(s) 1043, 1217
PagI TCATGA 1 cut(s) 712
PceI AGGCCT 2 cut(s) 612, 1011
PciI ACATGT 1 cut(s) 921
PfeI GAWTC 4 cut(s) 575, 586, 700, 727
PflFI GACNNNGTC 1 cut(s) 87
PflMI CCANNNNNTGG 1 cut(s) 848
PkrI GCNGC 5 cut(s) 502, 544, 1207, 1210, 1219
Ppu21I YACGTR 1 cut(s) 649
PscI ACATGT 1 cut(s) 921
Psp6I CCWGG 4 cut(s) 336, 906, 996, 1378
PspGI CCWGG 4 cut(s) 336, 906, 996, 1378
PspN4I GGNNCC 8 cut(s) 21, 51, 117, 143, 527, 563, 1377, 1471
PspPI GGNCC 7 cut(s) 78, 116, 326, 983, 1000, 1320, 1375
PstNI CAGNNNCTG 1 cut(s) 848
PsuI RGATCY 8 cut(s) 49, 135, 195, 688, 733, 838, 1020, 1073
PsyI GACNNNGTC 1 cut(s) 87
RruI TCGCGA 1 cut(s) 1293
RsaI GTAC 3 cut(s) 475, 645, 1147
RsaNI GTAC 3 cut(s) 474, 644, 1146
RseI CAYNNNNRTG 5 cut(s) 153, 180, 1104, 1226, 1293
SaqAI TTAA 5 cut(s) 30, 506, 663, 1056, 1079
SatI GCNGC 5 cut(s) 501, 543, 1206, 1209, 1218
Sau96I GGNCC 7 cut(s) 78, 116, 326, 983, 1000, 1320, 1375
ScrFI CCNGG 6 cut(s) 87, 338, 908, 998, 1365, 1380
SduI GDGCHC 3 cut(s) 220, 280, 337
SfaNI GCATC 6 cut(s) 145, 449, 646, 1101, 1285, 1297
SfiI GGCCNNNNNGGCC 1 cut(s) 1008
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 5 cut(s) 153, 180, 1104, 1226, 1293
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SphI GCATGC 2 cut(s) 1043, 1217
SseBI AGGCCT 2 cut(s) 612, 1011
SsiI CCGC 7 cut(s) 387, 518, 632, 809, 914, 1437, 1473
SspI AATATT 1 cut(s) 369
SspMI CTAG 3 cut(s) 330, 1061, 1130
StuI AGGCCT 2 cut(s) 612, 1011
StyD4I CCNGG 6 cut(s) 85, 336, 906, 996, 1363, 1378
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 2 cut(s) 651, 1034
TaqI TCGA 4 cut(s) 69, 168, 202, 1255
TaqII GACCGA 1 cut(s) 881
TatI WGTACW 2 cut(s) 473, 643
TfiI GAWTC 4 cut(s) 575, 586, 700, 727
Tru1I TTAA 5 cut(s) 30, 506, 663, 1056, 1079
Tru9I TTAA 5 cut(s) 30, 506, 663, 1056, 1079
TscAI CASTG 2 cut(s) 187, 1437
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 5 cut(s) 500, 542, 1205, 1208, 1217
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 4 cut(s) 309, 471, 729, 1146
TspRI CASTG 2 cut(s) 187, 1437
Tth111I GACNNNGTC 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 848
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 3 cut(s) 33, 716, 812
XceI RCATGY 3 cut(s) 925, 1043, 1217
XspI CTAG 3 cut(s) 330, 1061, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.