RLG00000000227

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013910
Physical Location & Seq
Forward (+)
87860 .. 91036
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000227

Sequence Viewer

Length: 2424 bp
ATGAGGTCGGAAACTCTCAAGTACGGTTCTAAGGGAAGGAATTTACCCCCCTATTCCGACCGGGGAGAACAGGCCGTTCAGCAAGGGGATTCGGAAATTGCGGAGGCTTGGTTCGATCAAGCCGCCGAGTATTGGAAACAAGCTATAGCGCTTACTCCTGAGGAGGACTCAATGATTATTCGTTCGCCGGAACCAGAAGTTAAAATTTTGGTAGATAGGGATCCCGTAAAAACTTCTTTCGAGGAATGGGCCAGACCCGGTCATTTCTCAAGAACAATAGCTAAGGGGCCTGATACTACCACTTGGATCTGGAACCTACACGCTGATGCTCACGATTTCGATAGCCATACCAGTGATTTGGAGGAGATCTCTCGAAAAGTATTTAGTGCCCATTTTGGTCAACTCTCCATCATCTTTCTTTGGCTGAGTGGTATGTATTTCCACGGTGCTCGTTTTTCCAATTATGAAGCATGGCTAAGTGATCCTACTCACATTGGACCTAGTGCCCAGGTGGTTTGGCCAATAGTGGGTCAAGAAATATTGAATGGTGATGTAGGCGGGGGTTTCCGAGGAATACAAATAACCTCTGGGTTTTTTCAGCTTTGGCGAGCATCTGGAATAACTAATGAATTACAACTCTATTGTACTGCAATTGGTGCATTGGTCTTTGCTGCCTTAATGCTTTTTGCGGGTTGGTTCCATTATCACAAAGCTGCTCCGAAATTGGCTTGGTTCCAAGATGTAGAATCTATGTTGAATCACCATTTAGCAGGGCTATTAGGCCTTGGGTCTCTTGGTTGGGCGGGGCATCAAGTACACGTATCGTTACCAATTAACCAATTTCTAAACGCTGGAGTAGATCCTAAAGAGATTCCACTTCCTCATGAATTTATCTTGAATCGGGATCTTTTGGCTCAACTTTATCCCAGTTTTGCTGAGGGAGCGACCCCATTTTTCACCTTGAATTGGTCAAAATATGCGGAATTTCTTACTTTTCGTGGAGGATTAGATCCAGTAACTGGAGGTCTATGGCTGACCGATATTGCACACCATCATTTAGCTATTGCAATTCTTTTCCTGGTAGCGGGTCACATGTATAAGACCAATTGGGGCATTGGTCATGGTATAAAAGATATTTTAGAGGCTCATAAAGGCCCATTTACCGGCCAGGGCCATAAAGGCCTATATGAGATCCTAACAACGTCATGGCATGCTCAATTATCTCTTAACCTAGCTATGTTAGGATCTTTAACCATTATTGTAGCTCACCATATGTATGCGATGCCCCCTTATCCATATCTAGCTACTGACTATGGTACACAACTTTCATTGTTCACACATCATATGTGGATTGGTGGATTTCTCATAGTTGGTGCTGCTGCGCATGCAGCCATTTTTATGGTAAGAGACTATGATCCAACTACTCGATACAACGATCTATTAGATCGTGTCCTTAGGCATCGCGATGCAATCATATCACATCTCAACTGGGTATGTATATTTTTAGGCTTTCACAGTTTTGGTTTGTATATTCATAATGATACCATGAGCGCGTTAGGGCGTCCCCAAGATATGTTTTCAGATACCGCTATACAATTACAGCCCGTCTTTGCTCAATGGATACAAAACACCCACGCTTTAGCACCCGGCGCAACGGCCCCTGGTGCAACAACAAGCACCAGTTTGACTTGGGGGGGTGGTGATTTAGTGGCAGTGGGCGGTAAAGTTGCTTTGTTACCTATTCCATTAGGAACCGCAGATTTTTTGGTACATCACATTCATGCATTTACAATTCATGTGACGGTATTGATACTCCTGAAAGGCGTTCTATTTGCTCGTAGTTCGCGTTTGATACCCGATAAAGCAAATCTTGGTTTTCGTTTTCCTTGTGATGGACCCGGAAGGGGGGGGACATGCCAAGTATCCGCTTGGGATCATGTCTTCTTAGGGCTATTCTGGATGTATAATGCAATTTCGGTAGTAATATTCCATTTCAGTTGGAAAATGCAATCAGATGTTTGGGGTAGTATAAGTGATCAAGGGGTGGTAACTCATATCACGGGAGGAAACTTTGCGCAGAGTTCCATTACTATTAATGGGTGGCTCCGCGATTTCTTATGGGCACAGGCATCCCAAGTAATTCAGTCCTACGGTTCTTCATTATCTGCATATGGCCTTTTTTTCCTAGGCGCCCATTTTGTATGGGCTTTTAGTTTAATGTTTCTATTCAGCGGACGTGGTTATTGGCAAGAACTTATTGAATCCATTGTTTGGGCTCATAATAAATTAAAAGTTGCTCCTGCTACTCAGCCTAGAGCCTTGAGTATTGTCCAAGGACGTGCTGTAGGAGTAACCCATTACCTTCTGGGTGGAATTGCCACAACATGGGCGTTCTTCTTAGCAAGAATTATTGCAGTAGGATAA

Protein Analysis

808

Amino Acids

89.49

Weight (kDa)

6.39

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaA_PsaB PF00223 89 - 801 0.0 Photosystem I psaA/psaB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1383, 2076
AccB1I GGYRCC 1 cut(s) 2189
AccB7I CCANNNNNTGG 3 cut(s) 1019, 2271, 2385
AccII CGCG 4 cut(s) 1464, 1553, 1846, 2109
AcoI YGGCCR 2 cut(s) 518, 1165
AcsI RAATTY 4 cut(s) 40, 204, 887, 983
AcyI GRCGYC 2 cut(s) 1561, 2190
AfaI GTAC 5 cut(s) 23, 646, 816, 1318, 1770
AfeI AGCGCT 1 cut(s) 150
AflIII ACRYGT 2 cut(s) 817, 1092
AgsI TTSAA 5 cut(s) 544, 757, 898, 964, 2261
AjiI CACGTC 2 cut(s) 2237, 2339
AjnI CCWGG 4 cut(s) 507, 1077, 1167, 1660
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 8 cut(s) 143, 281, 601, 713, 1061, 1235, 1265, 1304
AluI AGCT 8 cut(s) 143, 281, 601, 713, 1061, 1235, 1265, 1304
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 2 cut(s) 795, 1401
AlwNI CAGNNNCTG 1 cut(s) 1019
Aor51HI AGCGCT 1 cut(s) 150
ApeKI GCWGC 5 cut(s) 671, 713, 1376, 1379, 1388
ApoI RAATTY 4 cut(s) 40, 204, 887, 983
AseI ATTAAT 1 cut(s) 2094
AspA2I CCTAGG 1 cut(s) 2185
AspLEI GCGC 6 cut(s) 151, 1384, 1553, 1652, 2077, 2192
AspS9I GGNCC 7 cut(s) 249, 287, 497, 1154, 1171, 1657, 1895
AsuC2I CCSGG 4 cut(s) 62, 258, 1647, 1899
AsuHPI GGTGA 5 cut(s) 560, 752, 949, 1259, 1712
AvaII GGWCC 2 cut(s) 497, 1895
AvrII CCTAGG 1 cut(s) 2185
AxyI CCTNAGG 2 cut(s) 159, 1454
BaeGI GKGCMC 3 cut(s) 391, 508, 2125
BalI TGGCCA 1 cut(s) 520
BamHI GGATCC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 2189
BanII GRGCYC 1 cut(s) 2278
BbsI GAAGAC 1 cut(s) 1933
Bbv12I GWGCWC 1 cut(s) 451
BbvCI CCTCAGC 1 cut(s) 936
BbvI GCAGC 5 cut(s) 658, 700, 1363, 1366, 1400
BccI CCATC 3 cut(s) 416, 1059, 1886
BceAI ACGGC 2 cut(s) 59, 1671
BciT130I CCWGG 4 cut(s) 509, 1079, 1169, 1662
BciVI GTATCC 2 cut(s) 1614, 1933
BclI TGATCA 1 cut(s) 2035
BcnI CCSGG 4 cut(s) 62, 258, 1647, 1899
BcoDI GTCTC 2 cut(s) 795, 1401
BfaI CTAG 5 cut(s) 501, 1232, 1301, 2186, 2313
BfmI CTRYAG 2 cut(s) 144, 2343
BfoI RGCGCY 2 cut(s) 152, 2193
BfuI GTATCC 2 cut(s) 1614, 1933
BglI GCCNNNNNGGC 1 cut(s) 1179
BglII AGATCT 1 cut(s) 366
BisI GCNGC 6 cut(s) 123, 672, 714, 1377, 1380, 1389
BlnI CCTAGG 1 cut(s) 2185
BlsI GCNGC 6 cut(s) 124, 673, 715, 1378, 1381, 1390
Bme1390I CCNGG 8 cut(s) 62, 258, 509, 1079, 1169, 1647, 1662, 1899
Bme18I GGWCC 2 cut(s) 497, 1895
BmgBI CACGTC 2 cut(s) 2237, 2339
BmgT120I GGNCC 7 cut(s) 249, 287, 497, 1154, 1171, 1657, 1895
BmrFI CCNGG 8 cut(s) 62, 258, 509, 1079, 1169, 1647, 1662, 1899
BmrI ACTGGG 2 cut(s) 921, 1498
BmsI GCATC 7 cut(s) 316, 620, 817, 1272, 1456, 1468, 2138
BmuI ACTGGG 2 cut(s) 921, 1498
BpiI GAAGAC 1 cut(s) 1933
BplI GAGNNNNNCTC 4 cut(s) 152, 184, 353, 385
BpmI CTGGAG 2 cut(s) 873, 1041
Bpu10I CCTNAGC 2 cut(s) 282, 936
BpuEI CTTGAG 2 cut(s) 253, 2341
BpuMI CCSGG 4 cut(s) 62, 258, 1647, 1899
BsaAI YACGTR 1 cut(s) 820
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 1561, 2190
BsaI GGTCTC 1 cut(s) 795
BsaJI CCNNGG 9 cut(s) 61, 442, 507, 568, 784, 1168, 1660, 2185, 2332
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bse118I RCCGGY 1 cut(s) 1163
Bse1I ACTGG 6 cut(s) 351, 927, 1013, 1024, 1493, 1680
Bse21I CCTNAGG 2 cut(s) 159, 1454
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 4 cut(s) 509, 1079, 1169, 1662
BseDI CCNNGG 9 cut(s) 61, 442, 507, 568, 784, 1168, 1660, 2185, 2332
BseGI GGATG 2 cut(s) 1965, 2129
BseJI GATNNNNATC 1 cut(s) 219
BseMII CTCAG 4 cut(s) 150, 416, 927, 2321
BseNI ACTGG 6 cut(s) 351, 927, 1013, 1024, 1493, 1680
BseRI GAGGAG 2 cut(s) 176, 377
BseSI GKGCMC 3 cut(s) 391, 508, 2125
BseXI GCAGC 5 cut(s) 658, 700, 1363, 1366, 1400
Bsh1236I CGCG 4 cut(s) 1464, 1553, 1846, 2109
Bsh1285I CGRYCG 1 cut(s) 61
BshNI GGYRCC 1 cut(s) 2189
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 6 cut(s) 61, 188, 258, 1164, 1647, 1899
BslFI GGGAC 2 cut(s) 1548, 1924
BsmAI GTCTC 2 cut(s) 795, 1401
BsmFI GGGAC 2 cut(s) 1548, 1924
Bso31I GGTCTC 1 cut(s) 795
Bsp1286I GDGCHC 5 cut(s) 391, 451, 508, 2125, 2278
Bsp68I TCGCGA 1 cut(s) 1464
BspCNI CTCAG 4 cut(s) 151, 417, 928, 2320
BspFNI CGCG 4 cut(s) 1464, 1553, 1846, 2109
BspHI TCATGA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 2189
BspTNI GGTCTC 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 1163
BsrI ACTGG 6 cut(s) 351, 927, 1013, 1024, 1493, 1680
BssAI RCCGGY 1 cut(s) 1163
BssECI CCNNGG 9 cut(s) 61, 442, 507, 568, 784, 1168, 1660, 2185, 2332
BssNI GRCGYC 2 cut(s) 1561, 2190
BssT1I CCWWGG 3 cut(s) 784, 2185, 2332
Bst2UI CCWGG 4 cut(s) 509, 1079, 1169, 1662
Bst4CI ACNGT 5 cut(s) 26, 446, 1517, 1804, 2153
BstACI GRCGYC 2 cut(s) 1561, 2190
BstAPI GCANNNNNTGC 1 cut(s) 656
BstBAI YACGTR 1 cut(s) 820
BstC8I GCNNGC 3 cut(s) 609, 1212, 1386
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 2 cut(s) 1965, 2129
BstFNI CGCG 4 cut(s) 1464, 1553, 1846, 2109
BstH2I RGCGCY 2 cut(s) 152, 2193
BstHHI GCGC 6 cut(s) 151, 1384, 1553, 1652, 2077, 2192
BstMAI GTCTC 2 cut(s) 795, 1401
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 7 cut(s) 656, 941, 1179, 1385, 1388, 1649, 1664
BstNI CCWGG 4 cut(s) 509, 1079, 1169, 1662
BstNSI RCATGY 4 cut(s) 1096, 1214, 1388, 1917
BstSCI CCNGG 8 cut(s) 60, 256, 507, 1077, 1167, 1645, 1660, 1897
BstSFI CTRYAG 2 cut(s) 144, 2343
BstSLI GKGCMC 3 cut(s) 391, 508, 2125
BstUI CGCG 4 cut(s) 1464, 1553, 1846, 2109
BstV1I GCAGC 5 cut(s) 658, 700, 1363, 1366, 1400
BstV2I GAAGAC 1 cut(s) 1933
BstX2I RGATCY 8 cut(s) 220, 306, 366, 859, 904, 1009, 1191, 1244
BstXI CCANNNNNNTGG 2 cut(s) 358, 1399
BstYI RGATCY 8 cut(s) 220, 306, 366, 859, 904, 1009, 1191, 1244
Bsu36I CCTNAGG 2 cut(s) 159, 1454
BsuI GTATCC 2 cut(s) 1614, 1933
BtgI CCRYGG 1 cut(s) 442
BtgZI GCGATG 3 cut(s) 1295, 1445, 1479
BtrI CACGTC 2 cut(s) 2237, 2339
BtsCI GGATG 2 cut(s) 1965, 2129
BtsI GCAGTG 1 cut(s) 1719
BtsIMutI CAGTG 2 cut(s) 358, 1719
BtuMI TCGCGA 1 cut(s) 1464
Cac8I GCNNGC 3 cut(s) 609, 1212, 1386
CaiI CAGNNNCTG 1 cut(s) 1019
CciI TCATGA 1 cut(s) 883
CfoI GCGC 6 cut(s) 151, 1384, 1553, 1652, 2077, 2192
Cfr10I RCCGGY 1 cut(s) 1163
Cfr13I GGNCC 7 cut(s) 249, 287, 497, 1154, 1171, 1657, 1895
CseI GACGC 1 cut(s) 1550
Csp6I GTAC 5 cut(s) 22, 645, 815, 1317, 1769
CviQI GTAC 5 cut(s) 22, 645, 815, 1317, 1769
DinI GGCGCC 1 cut(s) 2191
EaeI YGGCCR 2 cut(s) 518, 1165
Eco130I CCWWGG 3 cut(s) 784, 2185, 2332
Eco147I AGGCCT 2 cut(s) 783, 1182
Eco24I GRGCYC 1 cut(s) 2278
Eco31I GGTCTC 1 cut(s) 795
Eco47I GGWCC 2 cut(s) 497, 1895
Eco47III AGCGCT 1 cut(s) 150
Eco81I CCTNAGG 2 cut(s) 159, 1454
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 4 cut(s) 507, 1077, 1167, 1660
EcoT14I CCWWGG 3 cut(s) 784, 2185, 2332
EcoT22I ATGCAT 1 cut(s) 1786
EcoT38I GRGCYC 1 cut(s) 2278
EgeI GGCGCC 1 cut(s) 2191
EheI GGCGCC 1 cut(s) 2191
ErhI CCWWGG 3 cut(s) 784, 2185, 2332
FalI AAGNNNNNCTT 2 cut(s) 1854, 1886
FaqI GGGAC 2 cut(s) 1548, 1924
FauI CCCGC 4 cut(s) 551, 682, 796, 1078
FauNDI CATATG 3 cut(s) 1272, 1344, 2170
FbaI TGATCA 1 cut(s) 2035
Fnu4HI GCNGC 6 cut(s) 123, 672, 714, 1377, 1380, 1389
FokI GGATG 2 cut(s) 1972, 2116
FriOI GRGCYC 1 cut(s) 2278
Fsp4HI GCNGC 6 cut(s) 123, 672, 714, 1377, 1380, 1389
FspBI CTAG 5 cut(s) 501, 1232, 1301, 2186, 2313
FspI TGCGCA 2 cut(s) 1383, 2076
GlaI GCGC 6 cut(s) 150, 1383, 1552, 1651, 2076, 2191
GluI GCNGC 6 cut(s) 123, 672, 714, 1377, 1380, 1389
GsuI CTGGAG 2 cut(s) 873, 1041
HaeII RGCGCY 2 cut(s) 152, 2193
HapII CCGG 6 cut(s) 61, 188, 258, 1164, 1647, 1899
HgaI GACGC 1 cut(s) 1550
HhaI GCGC 6 cut(s) 151, 1384, 1553, 1652, 2077, 2192
Hin1I GRCGYC 2 cut(s) 1561, 2190
Hin6I GCGC 6 cut(s) 149, 1382, 1551, 1650, 2075, 2190
HinP1I GCGC 6 cut(s) 149, 1382, 1551, 1650, 2075, 2190
HincII GTYRAC 1 cut(s) 401
HindII GTYRAC 1 cut(s) 401
HinfI GANTC 7 cut(s) 89, 167, 746, 757, 871, 898, 2261
HpaII CCGG 6 cut(s) 61, 188, 258, 1164, 1647, 1899
HphI GGTGA 5 cut(s) 560, 752, 949, 1259, 1712
Hpy166II GTNNAC 4 cut(s) 401, 817, 1319, 1335
Hpy188I TCNGA 7 cut(s) 10, 58, 94, 569, 720, 1582, 2014
Hpy8I GTNNAC 4 cut(s) 401, 817, 1319, 1335
HpyAV CCTTC 3 cut(s) 30, 1896, 2372
HpyCH4III ACNGT 5 cut(s) 26, 446, 1517, 1804, 2153
HpyCH4IV ACGT 4 cut(s) 819, 1202, 2236, 2338
HpyF10VI GCNNNNNNNGC 7 cut(s) 656, 941, 1179, 1385, 1388, 1649, 1664
HpySE526I ACGT 4 cut(s) 819, 1202, 2236, 2338
Hsp92I GRCGYC 2 cut(s) 1561, 2190
HspAI GCGC 6 cut(s) 149, 1382, 1551, 1650, 2075, 2190
KasI GGCGCC 1 cut(s) 2189
Ksp22I TGATCA 1 cut(s) 2035
LmnI GCTCC 4 cut(s) 721, 941, 2109, 2302
Lsp1109I GCAGC 5 cut(s) 658, 700, 1363, 1366, 1400
LweI GCATC 7 cut(s) 316, 620, 817, 1272, 1456, 1468, 2138
MaeI CTAG 5 cut(s) 501, 1232, 1301, 2186, 2313
MaeII ACGT 4 cut(s) 819, 1202, 2236, 2338
MaeIII GTNAC 7 cut(s) 825, 1015, 1088, 1734, 1798, 2047, 2350
MboII GAAGA 3 cut(s) 1933, 2148, 2386
MfeI CAATTG 2 cut(s) 651, 1105
MflI RGATCY 8 cut(s) 220, 306, 366, 859, 904, 1009, 1191, 1244
MhlI GDGCHC 5 cut(s) 391, 451, 508, 2125, 2278
MlsI TGGCCA 1 cut(s) 520
MluNI TGGCCA 1 cut(s) 520
Mly113I GGCGCC 1 cut(s) 2190
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 3 cut(s) 81, 1442, 1979
Mox20I TGGCCA 1 cut(s) 520
Mph1103I ATGCAT 1 cut(s) 1786
MscI TGGCCA 1 cut(s) 520
MseI TTAA 8 cut(s) 201, 677, 834, 1227, 1250, 2094, 2216, 2288
MslI CAYNNNNRTG 6 cut(s) 324, 351, 1275, 1397, 1464, 1779
Msp20I TGGCCA 1 cut(s) 520
MspA1I CMGCKG 1 cut(s) 2232
MspI CCGG 6 cut(s) 61, 188, 258, 1164, 1647, 1899
MspR9I CCNGG 8 cut(s) 62, 258, 509, 1079, 1169, 1647, 1662, 1899
MunI CAATTG 2 cut(s) 651, 1105
MvaI CCWGG 4 cut(s) 509, 1079, 1169, 1662
MvnI CGCG 4 cut(s) 1464, 1553, 1846, 2109
MwoI GCNNNNNNNGC 7 cut(s) 656, 941, 1179, 1385, 1388, 1649, 1664
NarI GGCGCC 1 cut(s) 2190
NciI CCSGG 4 cut(s) 62, 258, 1647, 1899
NdeI CATATG 3 cut(s) 1272, 1344, 2170
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 1088, 1798
NruI TCGCGA 1 cut(s) 1464
NsbI TGCGCA 2 cut(s) 1383, 2076
NsiI ATGCAT 1 cut(s) 1786
NspI RCATGY 4 cut(s) 1096, 1214, 1388, 1917
PaeI GCATGC 2 cut(s) 1214, 1388
PagI TCATGA 1 cut(s) 883
PceI AGGCCT 2 cut(s) 783, 1182
PciI ACATGT 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 1843
PfeI GAWTC 6 cut(s) 89, 746, 757, 871, 898, 2261
PflFI GACNNNGTC 1 cut(s) 258
PflMI CCANNNNNTGG 3 cut(s) 1019, 2271, 2385
PkrI GCNGC 6 cut(s) 124, 673, 715, 1378, 1381, 1390
PleI GAGTC 1 cut(s) 161
PluTI GGCGCC 1 cut(s) 2193
PpsI GAGTC 1 cut(s) 161
Ppu21I YACGTR 1 cut(s) 820
PscI ACATGT 1 cut(s) 1092
PshBI ATTAAT 1 cut(s) 2094
Psp6I CCWGG 4 cut(s) 507, 1077, 1167, 1660
PspGI CCWGG 4 cut(s) 507, 1077, 1167, 1660
PspPI GGNCC 7 cut(s) 249, 287, 497, 1154, 1171, 1657, 1895
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 8 cut(s) 220, 306, 366, 859, 904, 1009, 1191, 1244
PsyI GACNNNGTC 1 cut(s) 258
RruI TCGCGA 1 cut(s) 1464
RsaI GTAC 5 cut(s) 23, 646, 816, 1318, 1770
RsaNI GTAC 5 cut(s) 22, 645, 815, 1317, 1769
RseI CAYNNNNRTG 6 cut(s) 324, 351, 1275, 1397, 1464, 1779
SaqAI TTAA 8 cut(s) 201, 677, 834, 1227, 1250, 2094, 2216, 2288
SatI GCNGC 6 cut(s) 123, 672, 714, 1377, 1380, 1389
Sau96I GGNCC 7 cut(s) 249, 287, 497, 1154, 1171, 1657, 1895
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 8 cut(s) 62, 258, 509, 1079, 1169, 1647, 1662, 1899
SduI GDGCHC 5 cut(s) 391, 451, 508, 2125, 2278
SfaNI GCATC 7 cut(s) 316, 620, 817, 1272, 1456, 1468, 2138
SfcI CTRYAG 2 cut(s) 144, 2343
SfiI GGCCNNNNNGGCC 1 cut(s) 1179
SfoI GGCGCC 1 cut(s) 2191
SinI GGWCC 2 cut(s) 497, 1895
SmiMI CAYNNNNRTG 6 cut(s) 324, 351, 1275, 1397, 1464, 1779
SmlI CTYRAG 3 cut(s) 17, 268, 2320
SmoI CTYRAG 3 cut(s) 17, 268, 2320
SphI GCATGC 2 cut(s) 1214, 1388
SseBI AGGCCT 2 cut(s) 783, 1182
SspDI GGCGCC 1 cut(s) 2189
SspI AATATT 2 cut(s) 540, 1986
SspMI CTAG 5 cut(s) 501, 1232, 1301, 2186, 2313
StuI AGGCCT 2 cut(s) 783, 1182
StyD4I CCNGG 8 cut(s) 60, 256, 507, 1077, 1167, 1645, 1660, 1897
StyI CCWWGG 3 cut(s) 784, 2185, 2332
TaaI ACNGT 5 cut(s) 26, 446, 1517, 1804, 2153
TaiI ACGT 4 cut(s) 822, 1205, 2239, 2341
TaqI TCGA 5 cut(s) 114, 240, 339, 373, 1426
TaqII GACCGA 1 cut(s) 1052
TatI WGTACW 2 cut(s) 644, 814
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 6 cut(s) 89, 746, 757, 871, 898, 2261
Tru1I TTAA 8 cut(s) 201, 677, 834, 1227, 1250, 2094, 2216, 2288
Tru9I TTAA 8 cut(s) 201, 677, 834, 1227, 1250, 2094, 2216, 2288
TscAI CASTG 2 cut(s) 358, 1719
TseFI GTSAC 2 cut(s) 1088, 1798
TseI GCWGC 5 cut(s) 671, 713, 1376, 1379, 1388
Tsp45I GTSAC 2 cut(s) 1088, 1798
TspDTI ATGAA 8 cut(s) 480, 642, 900, 1317, 1523, 1769, 1784, 2148
TspRI CASTG 2 cut(s) 358, 1719
Tth111I GACNNNGTC 1 cut(s) 258
Van91I CCANNNNNTGG 3 cut(s) 1019, 2271, 2385
VpaK11BI GGWCC 2 cut(s) 497, 1895
VspI ATTAAT 1 cut(s) 2094
XapI RAATTY 4 cut(s) 40, 204, 887, 983
XceI RCATGY 4 cut(s) 1096, 1214, 1388, 1917
XmaJI CCTAGG 1 cut(s) 2185
XspI CTAG 5 cut(s) 501, 1232, 1301, 2186, 2313
Zsp2I ATGCAT 1 cut(s) 1786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.