RLG00000001200

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016900
Physical Location & Seq
Reverse (-)
11840535 .. 11841333
799 bp
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UTR
Exon/CDS
Intron
RLM00000001200

Sequence Viewer

Length: 312 bp
ATGCTCTATGAAGGCATTAGAGTGAAATGTCAGTACCGTCAAGACAGGGTGTACATGCGGAGTGCAGACTTCAGAGTGACTTTGAACAAAGAAATTCCCTCTGGAGCTGTTTCAGAGAAAGCAGGTGTTTATAGACAGGCAACCAGGGAGGTTGCTCTCATCTGTAATTTTCAGCGTACTGTCGCAAGGCCTGGTGGTGTACGGATGTACTGGTTAAAAGAGAACATGAGTGAGTTGCAGGGGCACATTGACGGAGCTGAAAACAGATTTGGACAGGGCAGTAATGGGATTACCCCCACTGAGAAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

104

Amino Acids

11.78

Weight (kDa)

9.51

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 16 - 86 1.2e-06 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 113
AciI CCGC 1 cut(s) 58
AcsI RAATTY 2 cut(s) 93, 305
AcuI CTGAAG 1 cut(s) 55
AfaI GTAC 5 cut(s) 35, 53, 178, 201, 209
AgsI TTSAA 1 cut(s) 85
AjnI CCWGG 2 cut(s) 143, 190
AjuI GAANNNNNNNTTGG 2 cut(s) 252, 284
AluBI AGCT 2 cut(s) 107, 257
AluI AGCT 2 cut(s) 107, 257
AoxI GGCC 1 cut(s) 188
ApoI RAATTY 2 cut(s) 93, 305
BaeGI GKGCMC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 145, 192
BfuAI ACCTGC 1 cut(s) 113
Bme1390I CCNGG 2 cut(s) 145, 192
BmrFI CCNGG 2 cut(s) 145, 192
BpmI CTGGAG 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 215
BseBI CCWGG 2 cut(s) 145, 192
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 215
BseSI GKGCMC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 1 cut(s) 190
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 246
Bsp1407I TGTACA 1 cut(s) 51
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 113
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 215
BssECI CCNNGG 1 cut(s) 144
Bst2UI CCWGG 2 cut(s) 145, 192
Bst4CI ACNGT 2 cut(s) 38, 181
BstAUI TGTACA 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 210
BstNI CCWGG 2 cut(s) 145, 192
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 143, 190
BstSLI GKGCMC 1 cut(s) 246
BsuRI GGCC 1 cut(s) 190
BtsCI GGATG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 113
Csp6I GTAC 5 cut(s) 34, 52, 177, 200, 208
CviAII CATG 2 cut(s) 55, 226
CviJI RGCY 3 cut(s) 107, 190, 257
CviKI_1 RGCY 3 cut(s) 107, 190, 257
CviQI GTAC 5 cut(s) 34, 52, 177, 200, 208
DdeI CTNAG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 143, 190
FaeI CATG 2 cut(s) 58, 229
FaiI YATR 4 cut(s) 9, 56, 132, 227
FatI CATG 2 cut(s) 54, 225
FokI GGATG 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 190
Hin1II CATG 2 cut(s) 58, 229
Hpy166II GTNNAC 2 cut(s) 52, 200
Hpy188I TCNGA 2 cut(s) 74, 115
Hpy188III TCNNGA 2 cut(s) 41, 102
Hpy8I GTNNAC 2 cut(s) 52, 200
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 2 cut(s) 38, 181
HpyCH4V TGCA 2 cut(s) 65, 238
HpyF3I CTNAG 1 cut(s) 300
Hsp92II CATG 2 cut(s) 58, 229
LmnI GCTCC 2 cut(s) 104, 254
MaeIII GTNAC 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 3 cut(s) 93, 166, 305
MnlI CCTC 2 cut(s) 109, 142
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 145, 192
MvaI CCWGG 2 cut(s) 145, 192
NlaIII CATG 2 cut(s) 58, 229
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 58
PaqCI CACCTGC 1 cut(s) 113
PceI AGGCCT 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 143, 190
PspGI CCWGG 2 cut(s) 143, 190
RsaI GTAC 5 cut(s) 35, 53, 178, 201, 209
RsaNI GTAC 5 cut(s) 34, 52, 177, 200, 208
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 145, 192
SduI GDGCHC 1 cut(s) 246
SetI ASST 4 cut(s) 109, 127, 153, 259
SmiMI CAYNNNNRTG 1 cut(s) 20
Sse9I AATT 3 cut(s) 93, 166, 305
SseBI AGGCCT 1 cut(s) 190
SsiI CCGC 1 cut(s) 58
StuI AGGCCT 1 cut(s) 190
StyD4I CCNGG 2 cut(s) 143, 190
TaaI ACNGT 2 cut(s) 38, 181
TasI AATT 3 cut(s) 93, 166, 305
TatI WGTACW 2 cut(s) 51, 207
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 24
TspGWI ACGGA 2 cut(s) 217, 267
TspRI CASTG 1 cut(s) 304
XapI RAATTY 2 cut(s) 93, 305
XceI RCATGY 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.