Rh7BG413800

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0016900
Physical Location & Seq
Forward (+)
46630079 .. 46638779
8701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG413800.1

Sequence Viewer

Length: 639 bp
ATGTCTTGGGAGGAATTAACTCGCCGTCTACAAGGAATTACAGCACATGATCTCGCCTTATTGGACATATATCATATAGATGACAGAACAAGTTGGATTCCAGTTTATAAAGTTATGGAGACTGCTGGGATGATCCGTTTAGATAGGACAGAGATCCGGGCCACCACCTTTTGTGAGACTACTGGAGTAATTGAAAGTGAGTTGAACAAAGAAACTCCCTCTGGAGCTGTTTCAGAGAAAGCAGGTGTTTATAGACAGGCAACCAGGGAGGTTGCTATTATCTGCAATTTTCAGCGTACTGTCGCAAGGCCTGGTGGTGTACGGATGTACTGGTTAAAAGAGAACATGAGTGAGTTGCAGGGCACATTGACAGAGCTGAAAACAGATTTGAACAGGGCAGTAATGGGATTGCCCCCACTGAGAAATTCTAATGGAAAACTAAAGAGAACCCTGACCTCAGAAGAGTTAGAGAGGACGATAGCTGAAACAATTCAGATGATTGAAAAATTGGAACAGGAGATGAGAACCCAAGAGAACCAGAAATGCATGTCAGTGGGAATGCCCAAAATCAAGTATCTTGACCCTAGGATCACTGTTGCATGGTGTAAGCGAAATGAAGTTCCTATTAACAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

212

Amino Acids

24.35

Weight (kDa)

8.32

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 62 - 129 4.1e-07 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 166 - 212 4.4e-15 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AarI CACCTGC 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 28
AclWI GGATC 3 cut(s) 127, 148, 596
AcsI RAATTY 1 cut(s) 424
AfaI GTAC 3 cut(s) 298, 321, 329
AgsI TTSAA 4 cut(s) 194, 205, 391, 503
AjnI CCWGG 2 cut(s) 263, 310
AluBI AGCT 3 cut(s) 227, 376, 482
AluI AGCT 3 cut(s) 227, 376, 482
Alw26I GTCTC 2 cut(s) 113, 170
AlwI GGATC 3 cut(s) 127, 148, 596
AoxI GGCC 2 cut(s) 159, 308
ApoI RAATTY 1 cut(s) 424
Asp700I GAANNNNTTC 1 cut(s) 489
AspA2I CCTAGG 1 cut(s) 584
AspS9I GGNCC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 158
AvrII CCTAGG 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 365
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 265, 312
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 2 cut(s) 113, 170
BfaI CTAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 233
BlnI CCTAGG 1 cut(s) 584
Bme1390I CCNGG 3 cut(s) 158, 265, 312
BmgT120I GGNCC 1 cut(s) 159
BmrFI CCNGG 3 cut(s) 158, 265, 312
BpmI CTGGAG 2 cut(s) 204, 243
BpuMI CCSGG 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 264, 584
Bse1I ACTGG 3 cut(s) 101, 187, 335
BseBI CCWGG 2 cut(s) 265, 312
BseDI CCNNGG 2 cut(s) 264, 584
BseGI GGATG 2 cut(s) 135, 330
BseMII CTCAG 2 cut(s) 410, 471
BseNI ACTGG 3 cut(s) 101, 187, 335
BseSI GKGCMC 1 cut(s) 365
BseYI CCCAGC 1 cut(s) 125
BshFI GGCC 2 cut(s) 161, 310
BsiSI CCGG 1 cut(s) 157
BsmAI GTCTC 2 cut(s) 113, 170
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 2 cut(s) 161, 310
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 4 cut(s) 49, 132, 153, 588
BspANI GGCC 2 cut(s) 161, 310
BspCNI CTCAG 2 cut(s) 411, 470
BspMI ACCTGC 1 cut(s) 233
BspPI GGATC 3 cut(s) 127, 148, 596
BsrI ACTGG 3 cut(s) 101, 187, 335
BssECI CCNNGG 2 cut(s) 264, 584
BssMI GATC 4 cut(s) 49, 132, 153, 588
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 265, 312
Bst4CI ACNGT 2 cut(s) 301, 595
Bst6I CTCTTC 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 419, 457
BstF5I GGATG 2 cut(s) 135, 330
BstKTI GATC 4 cut(s) 52, 135, 156, 591
BstMAI GTCTC 2 cut(s) 113, 170
BstMBI GATC 4 cut(s) 49, 132, 153, 588
BstNI CCWGG 2 cut(s) 265, 312
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 3 cut(s) 156, 263, 310
BstSLI GKGCMC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 2 cut(s) 161, 310
BtsCI GGATG 2 cut(s) 135, 330
BtsIMutI CAGTG 3 cut(s) 416, 558, 591
BveI ACCTGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 159
Csp6I GTAC 3 cut(s) 297, 320, 328
CviAII CATG 4 cut(s) 47, 346, 547, 600
CviJI RGCY 5 cut(s) 161, 227, 310, 376, 482
CviKI_1 RGCY 5 cut(s) 161, 227, 310, 376, 482
CviQI GTAC 3 cut(s) 297, 320, 328
DdeI CTNAG 2 cut(s) 419, 457
DpnI GATC 4 cut(s) 51, 134, 155, 590
DpnII GATC 4 cut(s) 49, 132, 153, 588
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 1 cut(s) 584
Eco147I AGGCCT 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 263, 310
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 584
FaeI CATG 4 cut(s) 50, 349, 550, 603
FatI CATG 4 cut(s) 46, 345, 546, 599
FblI GTMKAC 1 cut(s) 28
FokI GGATG 2 cut(s) 142, 337
FspBI CTAG 1 cut(s) 585
GsaI CCCAGC 1 cut(s) 129
GsuI CTGGAG 2 cut(s) 204, 243
HaeIII GGCC 2 cut(s) 161, 310
HapII CCGG 1 cut(s) 157
Hin1II CATG 4 cut(s) 50, 349, 550, 603
HinfI GANTC 1 cut(s) 97
HpaII CCGG 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 29, 320
Hpy188I TCNGA 3 cut(s) 235, 460, 495
Hpy188III TCNNGA 2 cut(s) 222, 578
Hpy8I GTNNAC 2 cut(s) 29, 320
HpyCH4III ACNGT 2 cut(s) 301, 595
HpyCH4V TGCA 4 cut(s) 285, 358, 546, 599
HpyF3I CTNAG 2 cut(s) 419, 457
Hsp92II CATG 4 cut(s) 50, 349, 550, 603
Kzo9I GATC 4 cut(s) 49, 132, 153, 588
LmnI GCTCC 1 cut(s) 224
MaeI CTAG 1 cut(s) 585
MalI GATC 4 cut(s) 51, 134, 155, 590
MboI GATC 4 cut(s) 49, 132, 153, 588
MboII GAAGA 1 cut(s) 473
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 7 cut(s) 14, 36, 189, 286, 424, 489, 506
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 5 cut(s) 4, 229, 262, 465, 466
Mph1103I ATGCAT 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 3 cut(s) 17, 335, 627
MslI CAYNNNNRTG 2 cut(s) 78, 551
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 3 cut(s) 158, 265, 312
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 2 cut(s) 265, 312
NciI CCSGG 1 cut(s) 158
NdeII GATC 4 cut(s) 49, 132, 153, 588
NlaIII CATG 4 cut(s) 50, 349, 550, 603
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 1 cut(s) 550
PaqCI CACCTGC 1 cut(s) 233
PceI AGGCCT 1 cut(s) 310
PctI GAATGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 1 cut(s) 97
PsiI TTATAA 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 263, 310
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 263, 310
PspPI GGNCC 1 cut(s) 159
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 3 cut(s) 298, 321, 329
RsaNI GTAC 3 cut(s) 297, 320, 328
RseI CAYNNNNRTG 2 cut(s) 78, 551
SaqAI TTAA 3 cut(s) 17, 335, 627
Sau3AI GATC 4 cut(s) 49, 132, 153, 588
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 3 cut(s) 158, 265, 312
SduI GDGCHC 1 cut(s) 365
SetI ASST 8 cut(s) 170, 229, 247, 273, 378, 458, 484, 636
SmiMI CAYNNNNRTG 2 cut(s) 78, 551
Sse9I AATT 7 cut(s) 14, 36, 189, 286, 424, 489, 506
SseBI AGGCCT 1 cut(s) 310
SspMI CTAG 1 cut(s) 585
StuI AGGCCT 1 cut(s) 310
StyD4I CCNGG 3 cut(s) 156, 263, 310
StyI CCWWGG 1 cut(s) 584
TaaI ACNGT 2 cut(s) 301, 595
TasI AATT 7 cut(s) 14, 36, 189, 286, 424, 489, 506
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 3 cut(s) 17, 335, 627
Tru9I TTAA 3 cut(s) 17, 335, 627
TscAI CASTG 3 cut(s) 423, 558, 598
TspDTI ATGAA 1 cut(s) 630
TspGWI ACGGA 2 cut(s) 125, 337
TspRI CASTG 3 cut(s) 423, 558, 598
XapI RAATTY 1 cut(s) 424
XceI RCATGY 1 cut(s) 550
XmaJI CCTAGG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 1 cut(s) 585
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.