Rh7AG441000

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0016900
Physical Location & Seq
Forward (+)
59756548 .. 59760875
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG441000.1

Sequence Viewer

Length: 1158 bp
ATGCCTCAGATTTTTGGAATGGAAAGTGCCAGGAAAAACTATAAGAAATCAAAGATGTCGGCCCAAAAAGAAAGAAAGAAAGCAAAGATGCTGATGATTTCTGAAATGAAAAGAGAGAAGGAGACATATGAGAAAGCAAGGATGCTGAAGGAGTACATAGAAAATATCAGGGCTGCATACACCAAGGATTTTACAAGTGAAGATGGTGAAAAGAGGCAGATGGCAGTTGCTACCTATCTTGTCGATAAACTTTGTCTGAGGGCTGGAAATGATACGGATGATGATGAAATTGATACTGTCGAGTGCTGCAAATTAAAAGTAGAGAACGTCAAAACAATTCCCCCTAACACGTTGGAGTTCAACTTCCTCGTTAAAGATTCAATAAGATGTGAAAATACTGTTGACGTTGAGCTTCCTGTTTACAAGGCAATCGAAGAGTTCCTGGCTGGAAAGAATGGTAGTGAAGGTCTATTTGACAAGTTGGAGAATACCAGTAAATTGAATGCGCATCTAGAGGGACTGATGCCTGGCCTTACAGGAAAAGTATTCCGTACATACAATGCATGCATTACATTGGATGAAATATTGAACAAAGAAACTCCCTCTGGAGCTGTTTCAGAGAAAGCAGGTGTTTATAGCCAGGCAACCAGGGAGGTTGCTATCATCTGTAATTTTCAGCGTACTGTCGCAAGGACTGGTAGTGTACGGATGTACTGGTTAAAAGAGTACATGAGTGAGTTGCAGGGCACATTGACGGAGCTGAAAACAGATTTGGACAGGGCGGTAATGGGATTACCCCCACTGAGAAATTCTAATCGAAAACCAAATAGAAACCTGACAGATATGGACAGGGCAGTAATCGGATTACCCCCACTGAGAAATTCTAATGGAAAACGAAAGAGAATCCTGACCCCAGAAGAGTTAGAGAGGACGATAGCTGAAACAATTCAGATGATTGAAAAATTGGAACAGGATATGAGAACCCAAGAGAACCAGAAATACACGTCAGTTGGAAGGCCCAAAATCAAGTATCTTGACCCTAGGATCGCTGTTGCATGGTGTAAGCGAAATGAAGTTCCTATTAACATGATATACACCGAGTATTATATGCCAATGTTTGAGTGGGCAATGGATGTCGATATCGACTTCAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

385

Amino Acids

44.49

Weight (kDa)

8.47

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 37 - 257 1.9e-68 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 318 - 385 8.7e-21 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 617
Acc16I TGCGCA 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 617
AciI CCGC 1 cut(s) 782
AclWI GGATC 1 cut(s) 1052
AcsI RAATTY 2 cut(s) 808, 880
AcuI CTGAAG 2 cut(s) 167, 1132
AfaI GTAC 6 cut(s) 155, 553, 682, 705, 713, 728
AflIII ACRYGT 2 cut(s) 348, 1002
AgsI TTSAA 5 cut(s) 361, 381, 502, 589, 959
AjiI CACGTC 1 cut(s) 1005
AjnI CCWGG 5 cut(s) 29, 441, 526, 639, 647
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 4 cut(s) 412, 611, 760, 938
AluI AGCT 4 cut(s) 412, 611, 760, 938
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 1 cut(s) 1052
AlwNI CAGNNNCTG 1 cut(s) 1155
AoxI GGCC 3 cut(s) 60, 529, 1016
ApeKI GCWGC 2 cut(s) 173, 306
ApoI RAATTY 2 cut(s) 808, 880
Asp700I GAANNNNTTC 1 cut(s) 945
AspA2I CCTAGG 1 cut(s) 1040
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 2 cut(s) 61, 1017
AsuHPI GGTGA 1 cut(s) 218
AvrII CCTAGG 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 749
BbvI GCAGC 2 cut(s) 160, 293
BccI CCATC 2 cut(s) 197, 214
BciT130I CCWGG 5 cut(s) 31, 443, 528, 641, 649
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 2 cut(s) 512, 1041
BfuAI ACCTGC 1 cut(s) 617
BisI GCNGC 2 cut(s) 174, 307
BlnI CCTAGG 1 cut(s) 1040
BlsI GCNGC 2 cut(s) 175, 308
Bme1390I CCNGG 5 cut(s) 31, 443, 528, 641, 649
BmgBI CACGTC 1 cut(s) 1005
BmgT120I GGNCC 2 cut(s) 61, 1017
BmrFI CCNGG 5 cut(s) 31, 443, 528, 641, 649
BmsI GCATC 4 cut(s) 78, 132, 513, 517
BpmI CTGGAG 1 cut(s) 627
BsaJI CCNNGG 3 cut(s) 183, 648, 1040
Bse1I ACTGG 3 cut(s) 492, 700, 719
Bse3DI GCAATG 1 cut(s) 1134
BseBI CCWGG 5 cut(s) 31, 443, 528, 641, 649
BseDI CCNNGG 3 cut(s) 183, 648, 1040
BseGI GGATG 5 cut(s) 147, 283, 583, 714, 1138
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 4 cut(s) 20, 248, 794, 866
BseNI ACTGG 3 cut(s) 492, 700, 719
BseSI GKGCMC 1 cut(s) 749
BseXI GCAGC 2 cut(s) 160, 293
BshFI GGCC 3 cut(s) 62, 531, 1018
BslFI GGGAC 1 cut(s) 531
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 531
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 3 cut(s) 62, 531, 1018
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 1 cut(s) 1044
BspACI CCGC 1 cut(s) 782
BspANI GGCC 3 cut(s) 62, 531, 1018
BspCNI CTCAG 4 cut(s) 19, 249, 795, 867
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 1 cut(s) 1052
BsrDI GCAATG 1 cut(s) 1134
BsrI ACTGG 3 cut(s) 492, 700, 719
BssECI CCNNGG 3 cut(s) 183, 648, 1040
BssMI GATC 1 cut(s) 1044
BssT1I CCWWGG 2 cut(s) 183, 1040
Bst2UI CCWGG 5 cut(s) 31, 443, 528, 641, 649
Bst4CI ACNGT 3 cut(s) 298, 400, 685
Bst6I CTCTTC 2 cut(s) 429, 912
BstC8I GCNNGC 1 cut(s) 565
BstDEI CTNAG 4 cut(s) 6, 257, 803, 875
BstF5I GGATG 5 cut(s) 147, 283, 583, 714, 1138
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 1 cut(s) 1047
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 1 cut(s) 1044
BstNI CCWGG 5 cut(s) 31, 443, 528, 641, 649
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 5 cut(s) 29, 441, 526, 639, 647
BstSLI GKGCMC 1 cut(s) 749
BstV1I GCAGC 2 cut(s) 160, 293
BsuRI GGCC 3 cut(s) 62, 531, 1018
BtrI CACGTC 1 cut(s) 1005
BtsCI GGATG 5 cut(s) 147, 283, 583, 714, 1138
BtsIMutI CAGTG 2 cut(s) 800, 872
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 1 cut(s) 565
CaiI CAGNNNCTG 1 cut(s) 1155
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 61, 1017
Csp6I GTAC 6 cut(s) 154, 552, 681, 704, 712, 727
CviAII CATG 4 cut(s) 564, 730, 1056, 1087
CviQI GTAC 6 cut(s) 154, 552, 681, 704, 712, 727
DdeI CTNAG 4 cut(s) 6, 257, 803, 875
DpnI GATC 1 cut(s) 1046
DpnII GATC 1 cut(s) 1044
Eam1104I CTCTTC 2 cut(s) 429, 912
EarI CTCTTC 2 cut(s) 429, 912
Eco130I CCWWGG 2 cut(s) 183, 1040
Eco32I GATATC 1 cut(s) 1141
Eco57I CTGAAG 2 cut(s) 167, 1132
EcoRII CCWGG 5 cut(s) 29, 441, 526, 639, 647
EcoRV GATATC 1 cut(s) 1141
EcoT14I CCWWGG 2 cut(s) 183, 1040
EcoT22I ATGCAT 2 cut(s) 565, 569
ErhI CCWWGG 2 cut(s) 183, 1040
FaeI CATG 4 cut(s) 567, 733, 1059, 1090
FaqI GGGAC 1 cut(s) 531
FatI CATG 4 cut(s) 563, 729, 1055, 1086
FauNDI CATATG 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 174, 307
FokI GGATG 5 cut(s) 154, 290, 590, 721, 1145
Fsp4HI GCNGC 2 cut(s) 174, 307
FspAI RTGCGCAY 1 cut(s) 507
FspBI CTAG 2 cut(s) 512, 1041
FspI TGCGCA 1 cut(s) 507
GlaI GCGC 1 cut(s) 507
GluI GCNGC 2 cut(s) 174, 307
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 3 cut(s) 62, 531, 1018
HhaI GCGC 1 cut(s) 508
Hin1II CATG 4 cut(s) 567, 733, 1059, 1090
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 3 cut(s) 377, 903, 1152
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 3 cut(s) 403, 421, 704
Hpy188I TCNGA 8 cut(s) 9, 103, 258, 619, 863, 951, 1151, 1157
Hpy188III TCNNGA 4 cut(s) 512, 606, 907, 1034
Hpy8I GTNNAC 3 cut(s) 403, 421, 704
HpyAV CCTTC 4 cut(s) 112, 142, 458, 1008
HpyCH4III ACNGT 3 cut(s) 298, 400, 685
HpyCH4IV ACGT 4 cut(s) 327, 350, 405, 1004
HpyCH4V TGCA 6 cut(s) 176, 309, 563, 567, 742, 1055
HpyF3I CTNAG 4 cut(s) 6, 257, 803, 875
HpySE526I ACGT 4 cut(s) 327, 350, 405, 1004
Hsp92II CATG 4 cut(s) 567, 733, 1059, 1090
HspAI GCGC 1 cut(s) 506
Kzo9I GATC 1 cut(s) 1044
LmnI GCTCC 2 cut(s) 608, 757
Lsp1109I GCAGC 2 cut(s) 160, 293
LweI GCATC 4 cut(s) 78, 132, 513, 517
MaeI CTAG 2 cut(s) 512, 1041
MaeII ACGT 4 cut(s) 327, 350, 405, 1004
MalI GATC 1 cut(s) 1046
MboI GATC 1 cut(s) 1044
MboII GAAGA 3 cut(s) 212, 446, 929
MhlI GDGCHC 1 cut(s) 749
MluCI AATT 9 cut(s) 288, 311, 336, 497, 670, 808, 880, 945, 962
MmeI TCCRAC 3 cut(s) 333, 462, 991
MnlI CCTC 8 cut(s) 15, 207, 252, 377, 508, 613, 646, 921
Mph1103I ATGCAT 2 cut(s) 565, 569
MroXI GAANNNNTTC 1 cut(s) 945
MseI TTAA 4 cut(s) 314, 372, 719, 1083
MspR9I CCNGG 5 cut(s) 31, 443, 528, 641, 649
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 5 cut(s) 31, 443, 528, 641, 649
NdeI CATATG 1 cut(s) 127
NdeII GATC 1 cut(s) 1044
NlaIII CATG 4 cut(s) 567, 733, 1059, 1090
NsbI TGCGCA 1 cut(s) 507
NsiI ATGCAT 2 cut(s) 565, 569
NspI RCATGY 1 cut(s) 567
PaeI GCATGC 1 cut(s) 567
PaqCI CACCTGC 1 cut(s) 617
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 945
PfeI GAWTC 3 cut(s) 377, 903, 1152
PkrI GCNGC 2 cut(s) 175, 308
Psp6I CCWGG 5 cut(s) 29, 441, 526, 639, 647
PspGI CCWGG 5 cut(s) 29, 441, 526, 639, 647
PspPI GGNCC 2 cut(s) 61, 1017
PsrI GAACNNNNNNTAC 2 cut(s) 983, 1015
PstNI CAGNNNCTG 1 cut(s) 1155
RsaI GTAC 6 cut(s) 155, 553, 682, 705, 713, 728
RsaNI GTAC 6 cut(s) 154, 552, 681, 704, 712, 727
SaqAI TTAA 4 cut(s) 314, 372, 719, 1083
SatI GCNGC 2 cut(s) 174, 307
Sau3AI GATC 1 cut(s) 1044
Sau96I GGNCC 2 cut(s) 61, 1017
ScrFI CCNGG 5 cut(s) 31, 443, 528, 641, 649
SduI GDGCHC 1 cut(s) 749
SfaNI GCATC 4 cut(s) 78, 132, 513, 517
SphI GCATGC 1 cut(s) 567
Sse9I AATT 9 cut(s) 288, 311, 336, 497, 670, 808, 880, 945, 962
SsiI CCGC 1 cut(s) 782
SspI AATATT 1 cut(s) 585
SspMI CTAG 2 cut(s) 512, 1041
StyD4I CCNGG 5 cut(s) 29, 441, 526, 639, 647
StyI CCWWGG 2 cut(s) 183, 1040
TaaI ACNGT 3 cut(s) 298, 400, 685
TaiI ACGT 4 cut(s) 330, 353, 408, 1007
TaqI TCGA 6 cut(s) 243, 300, 432, 817, 1137, 1143
TasI AATT 9 cut(s) 288, 311, 336, 497, 670, 808, 880, 945, 962
TatI WGTACW 3 cut(s) 153, 711, 726
TfiI GAWTC 3 cut(s) 377, 903, 1152
Tru1I TTAA 4 cut(s) 314, 372, 719, 1083
Tru9I TTAA 4 cut(s) 314, 372, 719, 1083
TscAI CASTG 2 cut(s) 807, 879
TseI GCWGC 2 cut(s) 173, 306
TspDTI ATGAA 4 cut(s) 122, 300, 594, 1086
TspGWI ACGGA 4 cut(s) 290, 539, 721, 770
TspRI CASTG 2 cut(s) 807, 879
XapI RAATTY 2 cut(s) 808, 880
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 567
XcmI CCANNNNNNNNNTGG 1 cut(s) 1119
XmaJI CCTAGG 1 cut(s) 1040
XmnI GAANNNNTTC 1 cut(s) 945
XspI CTAG 2 cut(s) 512, 1041
Zsp2I ATGCAT 2 cut(s) 565, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.