Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0006618

Physical Location & Seq

Genomic Coordinates Reverse (-)

26666520 .. 26669065

Gene Structure 2546 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002167

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGAGAATGGCTGCTGCTGTCACCACTTCAAGGGCCTTTCTTTACTCCCATCATTACCACAGCCTCCCCTTCCTTCTTCCTTCTTTCTTCACTGCCTCTAGGGTACCCAGGTTTCACACGAGGAACCCCATCCTCCTCTCTGCTGTCAAGTGTTTGCACTCCTCTTCCAGGCGTCAAAATGTGGTTGATATTTTGGAAGAAAGAGGGTTGCTCGAGTCCTTGACCAGTGAGCAGCTCAGGCATGCCTGCTCCGACCCTAGTATCCCTCCTCTTAAGGTCTATTGCGGGTTTGACCCAACTGCCGAGTCATTGCACTTAGGAAACCTTCTTGGCCTTATAGTCCTCTCATGGTTCCAAAGATGTGGCCATCAAACCATAGCTTTGATCGGTGGCGCCACTGCCCGTGTTGGAGACCCTTCTGGCAAGAGTTTGGAGAGGCCGGAGCTCGATCTGGAGACTTTGTCACAGAACACCTCAGGTATTACCAATACCATTGGGAAAATTTTGGGGTCCAGCGGGGTTTCCATCTTGAACAATTATGATTGGTTGAAAGAGGTTACGTTGCTGGATTTTCTGAAAGAAGTCGGTCGAGTTGCGAGGGTGGGCAGCATGATGGCCAAGGAGAGTGTGAAGAAGAGGTTGGAGTCAGAACAAGGAATGAGCTATACAGAGTTCACTTACCAGTTGTTGCAGGGCTATGATTTCCTCTACCTCTTCCGCAATGAAGGCACCAATGTTCAGATTGGAGGTAGCGATCAATGGGGAAATATAACTGCAGGGACTGAACTCATAAGGAAGATTCTTCGGGCTGATGATGCGGCTTACGGCTTGACATTCCGTCTTCTCTTAAAGAGTGACGGTACCAAATTCGGCAAGTCTGAAGATGGTGCCATATGGCTTTCCCCATCCATGTTGTCTCCCTACAAATTTTATCAGTATTTCTTCTCTGTTTCTGATGCTGATGTTGTCAGATTCCTCAAGATTCTCACTTTCCTAGACATGAACGACATCAAACAGATAGAGAGTGACATGAAGAGACCTGGATACCTACCCAACACCGCGCAGCGCAGGCTTGCTGAAGAGGTTACTCGTTTTGTCCATGGTCAAGATGGTCTAGATGAGGCTCTCAAGGCAACTGAAGCATTGAGGCCTGGCGCTGACACTAAATTAGACTGGAACACCATGCAAGCCATTTCAGAGGATGTCCCCTCCTGCTCTTTCCCTTATGATCAGGTCCTAGATCTTTCCGTTGTTGATCTTTCAGTGTCATCCGGTTTGCTTGACAGCAAATCCGCTGCCCGACGTCTATTGAAGCAAGGGGGGCTTTACTTGAACAATTCAAGAGTTGATAGTGAAAATAAGAGAATTGAATCTCAAGACATTCTGAGGTGCCCGCCTGATGCGGATGCTGTTGTTATCCTCATCCCGGCAGACTCTATTCCAATAGAGATGGGAATAGAGGAACTGATGCAAAATGTGTATGCAACCTGCTTTCAGCTGTTTAGTATGTCTAATCGTGCCCAATCTATCTCTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004831 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016874 GO:0016875 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034660 GO:0043038 GO:0043039 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

S4 tRNA-synt_1b

Protein Analysis

513

Amino Acids

57.02

Weight (kDa)

5.71

Isoelectric Point (pI)

49.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	90 - 381	8.8e-76	tRNA synthetases class I (W and Y)
SYY_C-terminal	PF22421	397 - 461	8.5e-06	Tyrosine--tRNA ligase SYY-like C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006618)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02660 AT3G02660
fragaria_vesca	FvH4_5g28930 FvH4_5g28930 FvH4_6g39550
malus_domestica	MD08G1236200.v1.1 MD15G1428200.v1.1
prunus_persica	Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1 Prupe.1G572900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0221241
rosa_laevigata	RLG00000002167
rosa_multiflora	Rmu_sc0000324.1_g000017 Rmu_sc0032411.1_g000002
rosa_roxburghii	Rroxscaffold_3G00238530
rosa_rugosa	Rorug07G0201700
rosa_samantha	Rh7AG340600 Rh7BG331800 Rh7CG358500 Rh7DG339400
rosa_wichuraiana	Rw7G028990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1306
Acc36I	ACCTGC	1 cut(s)	1496
Acc65I	GGTACC	2 cut(s)	103, 860
AccB1I	GGYRCC	6 cut(s)	103, 392, 728, 860, 887, 1389
AccII	CGCG	1 cut(s)	1061
AciI	CCGC	8 cut(s)	285, 516, 718, 818, 1059, 1293, 1394, 1403
AcoI	YGGCCR	2 cut(s)	364, 615
AcsI	RAATTY	3 cut(s)	501, 866, 926
AcuI	CTGAAG	3 cut(s)	900, 1098, 1158
AcyI	GRCGYC	3 cut(s)	172, 393, 1303
AfaI	GTAC	2 cut(s)	105, 862
AfiI	CCNNNNNNNGG	3 cut(s)	30, 168, 1426
AflII	CTTAAG	1 cut(s)	272
AgsI	TTSAA	7 cut(s)	30, 532, 550, 1312, 1333, 1341, 1370
AhdI	GACNNNNNGTC	1 cut(s)	837
AjnI	CCWGG	4 cut(s)	107, 167, 1039, 1150
AjuI	GAANNNNNNNTTGG	2 cut(s)	623, 655
AluBI	AGCT	5 cut(s)	235, 380, 445, 663, 1498
AluI	AGCT	5 cut(s)	235, 380, 445, 663, 1498
Alw21I	GWGCWC	1 cut(s)	447
Alw26I	GTCTC	4 cut(s)	405, 449, 921, 1030
AlwNI	CAGNNNCTG	1 cut(s)	782
Ama87I	CYCGRG	1 cut(s)	212
AoxI	GGCC	6 cut(s)	33, 331, 364, 437, 615, 1148
ApeKI	GCWGC	6 cut(s)	11, 14, 232, 606, 1063, 1295
ApoI	RAATTY	3 cut(s)	501, 866, 926
Asp718I	GGTACC	2 cut(s)	103, 860
AspLEI	GCGC	4 cut(s)	395, 1063, 1068, 1157
AspS9I	GGNCC	3 cut(s)	33, 510, 1234
AsuC2I	CCSGG	1 cut(s)	1427
AsuHPI	GGTGA	1 cut(s)	13
AvaI	CYCGRG	1 cut(s)	212
AvaII	GGWCC	2 cut(s)	510, 1234
AxyI	CCTNAGG	1 cut(s)	475
BaeGI	GKGCMC	2 cut(s)	1394, 1522
BaeI	ACNNNNGTAYC	4 cut(s)	95, 128, 244, 277
BalI	TGGCCA	2 cut(s)	366, 617
BanI	GGYRCC	6 cut(s)	103, 392, 728, 860, 887, 1389
BanII	GRGCYC	1 cut(s)	447
BauI	CACGAG	1 cut(s)	118
BbsI	GAAGAC	1 cut(s)	833
Bbv12I	GWGCWC	1 cut(s)	447
BbvI	GCAGC	4 cut(s)	244, 618, 1075, 1282
BccI	CCATC	9 cut(s)	57, 137, 375, 533, 607, 878, 913, 1103, 1444
BceAI	ACGGC	1 cut(s)	841
BciT130I	CCWGG	4 cut(s)	109, 169, 1041, 1152
BciVI	GTATCC	2 cut(s)	272, 1037
BclI	TGATCA	1 cut(s)	1228
BcnI	CCSGG	1 cut(s)	1427
BcoDI	GTCTC	4 cut(s)	405, 449, 921, 1030
BfaI	CTAG	5 cut(s)	99, 258, 995, 1115, 1238
BfmI	CTRYAG	1 cut(s)	774
BfoI	RGCGCY	2 cut(s)	396, 1158
BfrI	CTTAAG	1 cut(s)	272
BfuAI	ACCTGC	1 cut(s)	1496
BfuI	GTATCC	2 cut(s)	272, 1037
BglII	AGATCT	1 cut(s)	1240
BisI	GCNGC	7 cut(s)	12, 15, 233, 607, 819, 1064, 1296
BlsI	GCNGC	7 cut(s)	13, 16, 234, 608, 820, 1065, 1297
Bme1390I	CCNGG	5 cut(s)	109, 169, 1041, 1152, 1427
Bme18I	GGWCC	2 cut(s)	510, 1234
BmeRI	GACNNNNNGTC	1 cut(s)	837
BmeT110I	CYCGRG	1 cut(s)	212
BmgT120I	GGNCC	3 cut(s)	33, 510, 1234
BmiI	GGNNCC	9 cut(s)	105, 125, 353, 394, 511, 730, 862, 889, 1391
BmrFI	CCNGG	5 cut(s)	109, 169, 1041, 1152, 1427
BmsI	GCATC	5 cut(s)	805, 946, 1390, 1396, 1458
BpiI	GAAGAC	1 cut(s)	833
BplI	GAGNNNNNCTC	2 cut(s)	195, 227
BpmI	CTGGAG	1 cut(s)	473
Bpu10I	CCTNAGC	1 cut(s)	236
BpuEI	CTTGAG	3 cut(s)	962, 1112, 1359
BpuMI	CCSGG	1 cut(s)	1427
BsaHI	GRCGYC	3 cut(s)	172, 393, 1303
BsaI	GGTCTC	2 cut(s)	405, 1030
BsaJI	CCNNGG	3 cut(s)	107, 618, 1099
BsaWI	WCCGGW	1 cut(s)	1271
BsaXI	ACNNNNNCTCC	2 cut(s)	446, 476
Bsc4I	CCNNNNNNNGG	3 cut(s)	30, 168, 1426
Bse1I	ACTGG	3 cut(s)	225, 682, 1178
Bse21I	CCTNAGG	1 cut(s)	475
Bse3DI	GCAATG	2 cut(s)	308, 727
BseBI	CCWGG	4 cut(s)	109, 169, 1041, 1152
BseDI	CCNNGG	3 cut(s)	107, 618, 1099
BseGI	GGATG	6 cut(s)	129, 905, 1207, 1268, 1411, 1422
BseLI	CCNNNNNNNGG	3 cut(s)	30, 168, 1426
BseMI	GCAATG	2 cut(s)	308, 727
BseMII	CTCAG	3 cut(s)	250, 489, 1376
BseNI	ACTGG	3 cut(s)	225, 682, 1178
BseRI	GAGGAG	3 cut(s)	125, 151, 258
BseSI	GKGCMC	2 cut(s)	1394, 1522
BseXI	GCAGC	4 cut(s)	244, 618, 1075, 1282
Bsh1236I	CGCG	1 cut(s)	1061
Bsh1285I	CGRYCG	1 cut(s)	589
BshFI	GGCC	6 cut(s)	35, 333, 366, 439, 617, 1150
BshNI	GGYRCC	6 cut(s)	103, 392, 728, 860, 887, 1389
BsiEI	CGRYCG	1 cut(s)	589
BsiHKAI	GWGCWC	1 cut(s)	447
BsiHKCI	CYCGRG	1 cut(s)	212
BsiSI	CCGG	3 cut(s)	440, 1272, 1427
BslFI	GGGAC	2 cut(s)	793, 1190
BslI	CCNNNNNNNGG	3 cut(s)	30, 168, 1426
BsmAI	GTCTC	4 cut(s)	405, 449, 921, 1030
BsmFI	GGGAC	2 cut(s)	793, 1190
BsnI	GGCC	6 cut(s)	35, 333, 366, 439, 617, 1150
Bso31I	GGTCTC	2 cut(s)	405, 1030
BsoBI	CYCGRG	1 cut(s)	212
Bsp1286I	GDGCHC	3 cut(s)	447, 1394, 1522
Bsp143I	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
Bsp19I	CCATGG	1 cut(s)	1099
BspACI	CCGC	8 cut(s)	285, 516, 718, 818, 1059, 1293, 1394, 1403
BspANI	GGCC	6 cut(s)	35, 333, 366, 439, 617, 1150
BspCNI	CTCAG	3 cut(s)	249, 488, 1377
BspFNI	CGCG	1 cut(s)	1061
BspLI	GGNNCC	9 cut(s)	105, 125, 353, 394, 511, 730, 862, 889, 1391
BspMAI	CTGCAG	1 cut(s)	778
BspMI	ACCTGC	1 cut(s)	1496
BspT107I	GGYRCC	6 cut(s)	103, 392, 728, 860, 887, 1389
BspTI	CTTAAG	1 cut(s)	272
BspTNI	GGTCTC	2 cut(s)	405, 1030
BsrDI	GCAATG	2 cut(s)	308, 727
BsrI	ACTGG	3 cut(s)	225, 682, 1178
BssECI	CCNNGG	3 cut(s)	107, 618, 1099
BssMI	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
BssNI	GRCGYC	3 cut(s)	172, 393, 1303
BssSI	CACGAG	1 cut(s)	118
BssT1I	CCWWGG	2 cut(s)	618, 1099
Bst2BI	CACGAG	1 cut(s)	118
Bst2UI	CCWGG	4 cut(s)	109, 169, 1041, 1152
Bst4CI	ACNGT	1 cut(s)	860
Bst6I	CTCTTC	5 cut(s)	169, 629, 719, 1028, 1074
BstACI	GRCGYC	3 cut(s)	172, 393, 1303
BstAFI	CTTAAG	1 cut(s)	272
BstC8I	GCNNGC	6 cut(s)	243, 247, 1070, 1074, 1188, 1394
BstDEI	CTNAG	4 cut(s)	236, 316, 475, 1385
BstDSI	CCRYGG	1 cut(s)	1099
BstENI	CCTNNNNNAGG	1 cut(s)	166
BstF5I	GGATG	6 cut(s)	129, 905, 1207, 1268, 1411, 1422
BstFNI	CGCG	1 cut(s)	1061
BstH2I	RGCGCY	2 cut(s)	396, 1158
BstHHI	GCGC	4 cut(s)	395, 1063, 1068, 1157
BstKTI	GATC	6 cut(s)	387, 451, 757, 1231, 1243, 1258
BstMAI	GTCTC	4 cut(s)	405, 449, 921, 1030
BstMBI	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
BstMCI	CGRYCG	1 cut(s)	589
BstMWI	GCNNNNNNNGC	7 cut(s)	238, 726, 815, 1069, 1130, 1139, 1321
BstNI	CCWGG	4 cut(s)	109, 169, 1041, 1152
BstNSI	RCATGY	1 cut(s)	245
BstSCI	CCNGG	5 cut(s)	107, 167, 1039, 1150, 1425
BstSFI	CTRYAG	1 cut(s)	774
BstSLI	GKGCMC	2 cut(s)	1394, 1522
BstUI	CGCG	1 cut(s)	1061
BstV1I	GCAGC	4 cut(s)	244, 618, 1075, 1282
BstV2I	GAAGAC	1 cut(s)	833
BstX2I	RGATCY	1 cut(s)	1240
BstXI	CCANNNNNNTGG	1 cut(s)	362
BstYI	RGATCY	1 cut(s)	1240
Bsu36I	CCTNAGG	1 cut(s)	475
BsuI	GTATCC	2 cut(s)	272, 1037
BsuRI	GGCC	6 cut(s)	35, 333, 366, 439, 617, 1150
BtgI	CCRYGG	1 cut(s)	1099
BtsCI	GGATG	6 cut(s)	129, 905, 1207, 1268, 1411, 1422
BtsI	GCAGTG	2 cut(s)	90, 396
BtsIMutI	CAGTG	4 cut(s)	90, 232, 396, 1269
BveI	ACCTGC	1 cut(s)	1496
Cac8I	GCNNGC	6 cut(s)	243, 247, 1070, 1074, 1188, 1394
CaiI	CAGNNNCTG	1 cut(s)	782
CfoI	GCGC	4 cut(s)	395, 1063, 1068, 1157
Cfr13I	GGNCC	3 cut(s)	33, 510, 1234
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	2 cut(s)	104, 861
CviAII	CATG	8 cut(s)	242, 348, 610, 910, 1000, 1030, 1100, 1183
CviQI	GTAC	2 cut(s)	104, 861
DdeI	CTNAG	4 cut(s)	236, 316, 475, 1385
DinI	GGCGCC	1 cut(s)	394
DpnI	GATC	6 cut(s)	386, 450, 756, 1230, 1242, 1257
DpnII	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
DriI	GACNNNNNGTC	1 cut(s)	837
EaeI	YGGCCR	2 cut(s)	364, 615
Eam1104I	CTCTTC	5 cut(s)	169, 629, 719, 1028, 1074
Eam1105I	GACNNNNNGTC	1 cut(s)	837
EarI	CTCTTC	5 cut(s)	169, 629, 719, 1028, 1074
Ecl136II	GAGCTC	1 cut(s)	445
Eco130I	CCWWGG	2 cut(s)	618, 1099
Eco147I	AGGCCT	1 cut(s)	1150
Eco24I	GRGCYC	1 cut(s)	447
Eco31I	GGTCTC	2 cut(s)	405, 1030
Eco47I	GGWCC	2 cut(s)	510, 1234
Eco53kI	GAGCTC	1 cut(s)	445
Eco57I	CTGAAG	3 cut(s)	900, 1098, 1158
Eco81I	CCTNAGG	1 cut(s)	475
Eco88I	CYCGRG	1 cut(s)	212
EcoICRI	GAGCTC	1 cut(s)	445
EcoNI	CCTNNNNNAGG	1 cut(s)	166
EcoO109I	RGGNCCY	2 cut(s)	33, 1234
EcoRII	CCWGG	4 cut(s)	107, 167, 1039, 1150
EcoT14I	CCWWGG	2 cut(s)	618, 1099
EcoT38I	GRGCYC	1 cut(s)	447
EgeI	GGCGCC	1 cut(s)	394
EheI	GGCGCC	1 cut(s)	394
ErhI	CCWWGG	2 cut(s)	618, 1099
FaeI	CATG	8 cut(s)	245, 351, 613, 913, 1003, 1033, 1103, 1186
FalI	AAGNNNNNCTT	2 cut(s)	1308, 1340
FaqI	GGGAC	2 cut(s)	793, 1190
FatI	CATG	8 cut(s)	241, 347, 609, 909, 999, 1029, 1099, 1182
FauI	CCCGC	3 cut(s)	278, 509, 1401
FauNDI	CATATG	1 cut(s)	893
FbaI	TGATCA	1 cut(s)	1228
Fnu4HI	GCNGC	7 cut(s)	12, 15, 233, 607, 819, 1064, 1296
FokI	GGATG	6 cut(s)	116, 892, 1214, 1255, 1409, 1418
FriOI	GRGCYC	1 cut(s)	447
Fsp4HI	GCNGC	7 cut(s)	12, 15, 233, 607, 819, 1064, 1296
FspBI	CTAG	5 cut(s)	99, 258, 995, 1115, 1238
GlaI	GCGC	4 cut(s)	394, 1062, 1067, 1156
GluI	GCNGC	7 cut(s)	12, 15, 233, 607, 819, 1064, 1296
GsuI	CTGGAG	1 cut(s)	473
HaeII	RGCGCY	2 cut(s)	396, 1158
HaeIII	GGCC	6 cut(s)	35, 333, 366, 439, 617, 1150
HapII	CCGG	3 cut(s)	440, 1272, 1427
HgaI	GACGC	1 cut(s)	161
HhaI	GCGC	4 cut(s)	395, 1063, 1068, 1157
Hin1I	GRCGYC	3 cut(s)	172, 393, 1303
Hin1II	CATG	8 cut(s)	245, 351, 613, 913, 1003, 1033, 1103, 1186
Hin6I	GCGC	4 cut(s)	393, 1061, 1066, 1155
HinP1I	GCGC	4 cut(s)	393, 1061, 1066, 1155
HinfI	GANTC	8 cut(s)	215, 305, 644, 799, 972, 982, 1370, 1433
HpaII	CCGG	3 cut(s)	440, 1272, 1427
HphI	GGTGA	1 cut(s)	13
Hpy166II	GTNNAC	1 cut(s)	675
Hpy188III	TCNNGA	7 cut(s)	452, 529, 979, 1106, 1115, 1341, 1376
Hpy8I	GTNNAC	1 cut(s)	675
Hpy99I	CGWCG	1 cut(s)	1305
HpyAV	CCTTC	6 cut(s)	79, 83, 90, 335, 426, 719
HpyCH4III	ACNGT	1 cut(s)	860
HpyCH4IV	ACGT	2 cut(s)	560, 1303
HpyCH4V	TGCA	7 cut(s)	157, 313, 691, 776, 1186, 1471, 1484
HpyF10VI	GCNNNNNNNGC	7 cut(s)	238, 726, 815, 1069, 1130, 1139, 1321
HpyF3I	CTNAG	4 cut(s)	236, 316, 475, 1385
HpySE526I	ACGT	2 cut(s)	560, 1303
Hsp92I	GRCGYC	3 cut(s)	172, 393, 1303
Hsp92II	CATG	8 cut(s)	245, 351, 613, 913, 1003, 1033, 1103, 1186
HspAI	GCGC	4 cut(s)	393, 1061, 1066, 1155
KasI	GGCGCC	1 cut(s)	392
KpnI	GGTACC	2 cut(s)	107, 864
Ksp22I	TGATCA	1 cut(s)	1228
Kzo9I	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
LmnI	GCTCC	2 cut(s)	254, 442
Lsp1109I	GCAGC	4 cut(s)	244, 618, 1075, 1282
LweI	GCATC	5 cut(s)	805, 946, 1390, 1396, 1458
MaeI	CTAG	5 cut(s)	99, 258, 995, 1115, 1238
MaeII	ACGT	2 cut(s)	560, 1303
MaeIII	GTNAC	6 cut(s)	19, 462, 556, 854, 1025, 1084
MalI	GATC	6 cut(s)	386, 450, 756, 1230, 1242, 1257
MboI	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
MflI	RGATCY	1 cut(s)	1240
MhlI	GDGCHC	3 cut(s)	447, 1394, 1522
MlsI	TGGCCA	2 cut(s)	366, 617
MluCI	AATT	7 cut(s)	501, 535, 866, 926, 1166, 1336, 1365
MluNI	TGGCCA	2 cut(s)	366, 617
Mly113I	GGCGCC	1 cut(s)	393
MlyI	GAGTC	4 cut(s)	224, 314, 653, 1427
MmeI	TCCRAC	3 cut(s)	276, 388, 621
Mox20I	TGGCCA	2 cut(s)	366, 617
MscI	TGGCCA	2 cut(s)	366, 617
MseI	TTAA	3 cut(s)	273, 848, 1537
Msp20I	TGGCCA	2 cut(s)	366, 617
MspA1I	CMGCKG	3 cut(s)	516, 1295, 1498
MspCI	CTTAAG	1 cut(s)	272
MspI	CCGG	3 cut(s)	440, 1272, 1427
MspR9I	CCNGG	5 cut(s)	109, 169, 1041, 1152, 1427
MteI	GCGCNGCGC	1 cut(s)	1064
MvaI	CCWGG	4 cut(s)	109, 169, 1041, 1152
MvnI	CGCG	1 cut(s)	1061
MwoI	GCNNNNNNNGC	7 cut(s)	238, 726, 815, 1069, 1130, 1139, 1321
NarI	GGCGCC	1 cut(s)	393
NciI	CCSGG	1 cut(s)	1427
NcoI	CCATGG	1 cut(s)	1099
NdeI	CATATG	1 cut(s)	893
NdeII	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
NlaIII	CATG	8 cut(s)	245, 351, 613, 913, 1003, 1033, 1103, 1186
NlaIV	GGNNCC	9 cut(s)	105, 125, 353, 394, 511, 730, 862, 889, 1391
NmeAIII	GCCGAG	1 cut(s)	328
NmuCI	GTSAC	4 cut(s)	19, 462, 854, 1025
NspI	RCATGY	1 cut(s)	245
PaeI	GCATGC	1 cut(s)	245
PaeR7I	CTCGAG	1 cut(s)	212
PceI	AGGCCT	1 cut(s)	1150
PfeI	GAWTC	4 cut(s)	799, 972, 982, 1370
PflFI	GACNNNGTC	1 cut(s)	460
PkrI	GCNGC	7 cut(s)	13, 16, 234, 608, 820, 1065, 1297
PleI	GAGTC	4 cut(s)	223, 313, 652, 1427
PluTI	GGCGCC	1 cut(s)	396
PpsI	GAGTC	4 cut(s)	223, 313, 652, 1427
PpuMI	RGGWCCY	1 cut(s)	1234
Psp124BI	GAGCTC	1 cut(s)	447
Psp5II	RGGWCCY	1 cut(s)	1234
Psp6I	CCWGG	4 cut(s)	107, 167, 1039, 1150
PspGI	CCWGG	4 cut(s)	107, 167, 1039, 1150
PspN4I	GGNNCC	9 cut(s)	105, 125, 353, 394, 511, 730, 862, 889, 1391
PspPI	GGNCC	3 cut(s)	33, 510, 1234
PspPPI	RGGWCCY	1 cut(s)	1234
PspXI	VCTCGAGB	1 cut(s)	212
PstI	CTGCAG	1 cut(s)	778
PstNI	CAGNNNCTG	1 cut(s)	782
PsuI	RGATCY	1 cut(s)	1240
PsyI	GACNNNGTC	1 cut(s)	460
PvuII	CAGCTG	1 cut(s)	1498
RsaI	GTAC	2 cut(s)	105, 862
RsaNI	GTAC	2 cut(s)	104, 861
SacI	GAGCTC	1 cut(s)	447
SaqAI	TTAA	3 cut(s)	273, 848, 1537
SatI	GCNGC	7 cut(s)	12, 15, 233, 607, 819, 1064, 1296
Sau3AI	GATC	6 cut(s)	384, 448, 754, 1228, 1240, 1255
Sau96I	GGNCC	3 cut(s)	33, 510, 1234
SchI	GAGTC	4 cut(s)	224, 314, 653, 1427
ScrFI	CCNGG	5 cut(s)	109, 169, 1041, 1152, 1427
SduI	GDGCHC	3 cut(s)	447, 1394, 1522
SfaNI	GCATC	5 cut(s)	805, 946, 1390, 1396, 1458
SfcI	CTRYAG	1 cut(s)	774
SfoI	GGCGCC	1 cut(s)	394
Sfr274I	CTCGAG	1 cut(s)	212
SinI	GGWCC	2 cut(s)	510, 1234
SlaI	CTCGAG	1 cut(s)	212
SmlI	CTYRAG	5 cut(s)	212, 272, 977, 1127, 1374
SmoI	CTYRAG	5 cut(s)	212, 272, 977, 1127, 1374
SphI	GCATGC	1 cut(s)	245
Sse9I	AATT	7 cut(s)	501, 535, 866, 926, 1166, 1336, 1365
SseBI	AGGCCT	1 cut(s)	1150
SsiI	CCGC	8 cut(s)	285, 516, 718, 818, 1059, 1293, 1394, 1403
SspDI	GGCGCC	1 cut(s)	392
SspMI	CTAG	5 cut(s)	99, 258, 995, 1115, 1238
SstI	GAGCTC	1 cut(s)	447
StuI	AGGCCT	1 cut(s)	1150
StyD4I	CCNGG	5 cut(s)	107, 167, 1039, 1150, 1425
StyI	CCWWGG	2 cut(s)	618, 1099
TaaI	ACNGT	1 cut(s)	860
TaiI	ACGT	2 cut(s)	563, 1306
TaqI	TCGA	3 cut(s)	213, 447, 589
TaqII	GACCGA	1 cut(s)	575
TasI	AATT	7 cut(s)	501, 535, 866, 926, 1166, 1336, 1365
TauI	GCSGC	1 cut(s)	821
TfiI	GAWTC	4 cut(s)	799, 972, 982, 1370
Tru1I	TTAA	3 cut(s)	273, 848, 1537
Tru9I	TTAA	3 cut(s)	273, 848, 1537
TscAI	CASTG	4 cut(s)	97, 232, 403, 1269
TseFI	GTSAC	4 cut(s)	19, 462, 854, 1025
TseI	GCWGC	6 cut(s)	11, 14, 232, 606, 1063, 1295
Tsp45I	GTSAC	4 cut(s)	19, 462, 854, 1025
TspDTI	ATGAA	3 cut(s)	738, 1016, 1046
TspGWI	ACGGA	2 cut(s)	827, 1237
TspRI	CASTG	4 cut(s)	97, 232, 403, 1269
Tth111I	GACNNNGTC	1 cut(s)	460
Vha464I	CTTAAG	1 cut(s)	272
VpaK11BI	GGWCC	2 cut(s)	510, 1234
XagI	CCTNNNNNAGG	1 cut(s)	166
XapI	RAATTY	3 cut(s)	501, 866, 926
XbaI	TCTAGA	1 cut(s)	1114
XceI	RCATGY	1 cut(s)	245
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1106
XhoI	CTCGAG	1 cut(s)	212
XspI	CTAG	5 cut(s)	99, 258, 995, 1115, 1238
ZraI	GACGTC	1 cut(s)	1304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.