RLG00000002423

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0020486
Physical Location & Seq
Reverse (-)
33870229 .. 33872691
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002423

Sequence Viewer

Length: 573 bp
ATGAAGAACACAGATGCAAAGGAGGAAGAACACAGATGTAATGGAGGTCGGAGATGGCCGAATATTGCCTGGCGATTGCCGGATTTTAGCCGACGAGTTCTTGACTTCTTGCCGGAAACAAAAATAGCAGAGGTCGAATTCAACATCTTCATACCGGCGGAGACGCATCAGATATTGGAGAAATCAGTGGAGAACTACCAGAAAGAAGTCTTAAGTGTGGTTATTGGGGTTATACTAGAGCTCGGTGGAGGAGCCGATATAGGCTTCAATGATTTCTTCTTGGTCCACAACTTCAGTGGAGCGGAGGCGTCCGATAATGGCCTCGAACTTGATAAAGAAGTTGGGACATATCAACTCATCATTAGGTTCGATAAGGCGTCAAAAAGACATCTGATGATGCAAAATCAGATGATTGATGCTGGTGCACTAAATTTCTTTTGGTCTCAGAATTGTGGCGGTGCTGATACGCGACATGGATGGCTTCTGGAGATTGTGAAGACGGTGGCCATTGGCAGCGAAGGATATGGAGGCGGCTGGCCCCTTGGTGGTGTCATCAGCATGATAAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.25

Weight (kDa)

5.25

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020486)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0376751
rosa_laevigata RLG00000002423 RLG00000025737
rosa_roxburghii Rroxscaffold_3G00237280
rosa_samantha Rh4BG153200 Rh5BG374400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 302
AccII CGCG 1 cut(s) 469
AciI CCGC 4 cut(s) 158, 302, 456, 531
AcoI YGGCCR 2 cut(s) 56, 504
AcsI RAATTY 2 cut(s) 137, 430
AcuI CTGAAG 1 cut(s) 277
AcyI GRCGYC 2 cut(s) 308, 377
AfiI CCNNNNNNNGG 1 cut(s) 545
AflII CTTAAG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 142, 268
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw21I GWGCWC 2 cut(s) 243, 427
Alw26I GTCTC 2 cut(s) 155, 447
Alw44I GTGCAC 1 cut(s) 423
AoxI GGCC 4 cut(s) 56, 319, 504, 536
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 513
ApoI RAATTY 2 cut(s) 137, 430
AspS9I GGNCC 2 cut(s) 283, 537
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 427
BalI TGGCCA 1 cut(s) 506
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 243, 427
BbvI GCAGC 1 cut(s) 525
BccI CCATC 2 cut(s) 48, 471
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 155, 447
BfaI CTAG 1 cut(s) 236
BfrI CTTAAG 1 cut(s) 211
BisI GCNGC 2 cut(s) 514, 532
BlsI GCNGC 2 cut(s) 515, 533
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 283, 537
BmiI GGNNCC 2 cut(s) 253, 539
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 4 cut(s) 4, 175, 387, 406
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 506
BsaHI GRCGYC 2 cut(s) 308, 377
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 541
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse118I RCCGGY 1 cut(s) 154
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 541
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 1 cut(s) 545
BseMII CTCAG 1 cut(s) 458
BseRI GAGGAG 1 cut(s) 264
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 469
BshFI GGCC 4 cut(s) 58, 321, 506, 538
BsiHKAI GWGCWC 2 cut(s) 243, 427
BsiSI CCGG 3 cut(s) 80, 113, 155
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 155, 447
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 4 cut(s) 58, 321, 506, 538
Bso31I GGTCTC 1 cut(s) 447
Bsp1286I GDGCHC 2 cut(s) 243, 427
BspACI CCGC 4 cut(s) 158, 302, 456, 531
BspANI GGCC 4 cut(s) 58, 321, 506, 538
BspCNI CTCAG 1 cut(s) 457
BspFNI CGCG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 253, 539
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 447
BsrBI CCGCTC 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 541
BssNI GRCGYC 2 cut(s) 308, 377
BssT1I CCWWGG 1 cut(s) 541
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 502
BstACI GRCGYC 2 cut(s) 308, 377
BstAFI CTTAAG 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 482
BstFNI CGCG 1 cut(s) 469
BstMAI GTCTC 2 cut(s) 155, 447
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 1 cut(s) 469
BstV1I GCAGC 1 cut(s) 525
BstV2I GAAGAC 1 cut(s) 503
BsuRI GGCC 4 cut(s) 58, 321, 506, 538
BtsCI GGATG 1 cut(s) 482
BtsIMutI CAGTG 2 cut(s) 192, 301
Cac8I GCNNGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 283, 537
CseI GACGC 3 cut(s) 172, 297, 366
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 2 cut(s) 473, 559
DdeI CTNAG 1 cut(s) 444
EaeI YGGCCR 2 cut(s) 56, 504
EciI GGCGGA 1 cut(s) 173
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 541
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 2 cut(s) 476, 562
FaiI YATR 7 cut(s) 152, 233, 260, 349, 474, 525, 560
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 472, 558
Fnu4HI GCNGC 2 cut(s) 514, 532
FokI GGATG 1 cut(s) 489
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 514, 532
FspBI CTAG 1 cut(s) 236
GluI GCNGC 2 cut(s) 514, 532
GsuI CTGGAG 1 cut(s) 506
HaeIII GGCC 4 cut(s) 58, 321, 506, 538
HapII CCGG 3 cut(s) 80, 113, 155
HgaI GACGC 3 cut(s) 172, 297, 366
Hin1I GRCGYC 2 cut(s) 308, 377
Hin1II CATG 2 cut(s) 476, 562
HpaII CCGG 3 cut(s) 80, 113, 155
Hpy166II GTNNAC 2 cut(s) 286, 425
Hpy188I TCNGA 6 cut(s) 51, 171, 313, 393, 408, 447
Hpy188III TCNNGA 2 cut(s) 101, 485
Hpy8I GTNNAC 2 cut(s) 286, 425
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4V TGCA 3 cut(s) 17, 400, 425
HpyF3I CTNAG 1 cut(s) 444
Hsp92I GRCGYC 2 cut(s) 308, 377
Hsp92II CATG 2 cut(s) 476, 562
LmnI GCTCC 2 cut(s) 251, 299
LpnPI CCDG 9 cut(s) 55, 82, 93, 126, 168, 212, 405, 470, 520
Lsp1109I GCAGC 1 cut(s) 525
LweI GCATC 4 cut(s) 4, 175, 387, 406
MaeI CTAG 1 cut(s) 236
MbiI CCGCTC 1 cut(s) 302
MboII GAAGA 5 cut(s) 16, 38, 139, 268, 508
MhlI GDGCHC 2 cut(s) 243, 427
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 3 cut(s) 137, 430, 448
MluNI TGGCCA 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 29
MnlI CCTC 7 cut(s) 16, 38, 124, 242, 298, 332, 521
Mox20I TGGCCA 1 cut(s) 506
MscI TGGCCA 1 cut(s) 506
MseI TTAA 1 cut(s) 212
MslI CAYNNNNRTG 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 506
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 3 cut(s) 80, 113, 155
MspR9I CCNGG 1 cut(s) 70
MvaI CCWGG 1 cut(s) 70
MvnI CGCG 1 cut(s) 469
NlaIII CATG 2 cut(s) 476, 562
NlaIV GGNNCC 2 cut(s) 253, 539
PkrI GCNGC 2 cut(s) 515, 533
Psp124BI GAGCTC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 253, 539
PspPI GGNCC 2 cut(s) 283, 537
RseI CAYNNNNRTG 1 cut(s) 557
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 2 cut(s) 514, 532
Sau96I GGNCC 2 cut(s) 283, 537
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 2 cut(s) 243, 427
SetI ASST 4 cut(s) 49, 135, 243, 368
SfaNI GCATC 4 cut(s) 4, 175, 387, 406
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 3 cut(s) 137, 430, 448
SsiI CCGC 4 cut(s) 158, 302, 456, 531
SspI AATATT 1 cut(s) 64
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 68
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 502
TaqI TCGA 3 cut(s) 135, 324, 369
TasI AATT 3 cut(s) 137, 430, 448
TauI GCSGC 1 cut(s) 534
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 2 cut(s) 192, 301
TseI GCWGC 1 cut(s) 513
TspDTI ATGAA 2 cut(s) 17, 139
TspRI CASTG 2 cut(s) 192, 301
Vha464I CTTAAG 1 cut(s) 211
VneI GTGCAC 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 137, 430
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.