Rroxscaffold_3G00237280

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0020486
Physical Location & Seq
Forward (+)
24875758 .. 24876207
450 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00237280.1

Sequence Viewer

Length: 450 bp
ATGACCAAAATGCTTGAGTTTCATCACAATTTACGGGCATTGCCACCGACCCAATATCCAATACCCGGAAACGGAAAACCCAAGGAACCAACGCCAAATCTAACCACCTCCGCTTCCTTCTCCTCCTCCCACCCTTCATTCCTCTTCAACCCAACCACCACCGCTTCCACTTCCTCTCGCCATGTTCGTCTCTCAAGAACAAGACTAGGCGCCTCTAGAAGACCACCGTGCTGCGCCGCGAGCCTCAAGTGGCTCTTCAATAATCCCAAGAGCGACGACGACGGCAAGATTGAAAAAATCGGCGACGGAGGTGATGGTGATGATGATAATGAGGGCGAAAGTGGGTTGGCGCTTAAAGGTACCGACTTGGCGGGGCTGTTCTTGGTTGGGATTGTTGGTGGGTTTGGGCGGCTGGGTATTTGTATAAGGATCGAGTTAATGCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

149

Amino Acids

15.94

Weight (kDa)

8.52

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020486)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0376751
rosa_laevigata RLG00000002423 RLG00000025737
rosa_roxburghii Rroxscaffold_3G00237280
rosa_samantha Rh4BG153200 Rh5BG374400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 2 cut(s) 209, 359
AccII CGCG 1 cut(s) 239
AciI CCGC 5 cut(s) 111, 162, 237, 371, 409
AclWI GGATC 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 65, 71
AgsI TTSAA 3 cut(s) 148, 259, 293
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 231
Asp718I GGTACC 1 cut(s) 359
AspLEI GCGC 3 cut(s) 212, 236, 352
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 323, 329
BanI GGYRCC 2 cut(s) 209, 359
BbsI GAAGAC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 218
BccI CCATC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 298
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 2 cut(s) 206, 216
BfoI RGCGCY 2 cut(s) 213, 353
BisI GCNGC 3 cut(s) 232, 237, 410
BlsI GCNGC 3 cut(s) 233, 238, 411
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 87, 211, 361
BmrFI CCNGG 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 226
BpuEI CTTGAG 3 cut(s) 35, 178, 230
BpuMI CCSGG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 71
Bse3DI GCAATG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 65, 71
BseMI GCAATG 1 cut(s) 38
BseRI GAGGAG 2 cut(s) 112, 115
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 412
Bsh1236I CGCG 1 cut(s) 239
BshNI GGYRCC 2 cut(s) 209, 359
BsiSI CCGG 1 cut(s) 66
BslI CCNNNNNNNGG 2 cut(s) 65, 71
BsmAI GTCTC 1 cut(s) 194
BsmBI CGTCTC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 429
BspACI CCGC 5 cut(s) 111, 162, 237, 371, 409
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 3 cut(s) 87, 211, 361
BspPI GGATC 1 cut(s) 437
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 2 cut(s) 209, 359
BsrDI GCAATG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 429
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 2 cut(s) 149, 260
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 241
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 2 cut(s) 213, 353
BstHHI GCGC 3 cut(s) 212, 236, 352
BstKTI GATC 1 cut(s) 432
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 1 cut(s) 429
BstMWI GCNNNNNNNGC 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 64
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 3 cut(s) 212, 236, 352
Csp6I GTAC 1 cut(s) 360
CviAII CATG 1 cut(s) 182
CviJI RGCY 4 cut(s) 243, 253, 376, 412
CviKI_1 RGCY 4 cut(s) 243, 253, 376, 412
CviQI GTAC 1 cut(s) 360
DinI GGCGCC 1 cut(s) 211
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
Eam1104I CTCTTC 2 cut(s) 149, 260
EarI CTCTTC 2 cut(s) 149, 260
Eco130I CCWWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 1 cut(s) 185
FaiI YATR 2 cut(s) 183, 425
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 1 cut(s) 181
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 232, 237, 410
Fsp4HI GCNGC 3 cut(s) 232, 237, 410
FspBI CTAG 2 cut(s) 206, 216
GlaI GCGC 3 cut(s) 211, 235, 351
GluI GCNGC 3 cut(s) 232, 237, 410
GsaI CCCAGC 1 cut(s) 416
HaeII RGCGCY 2 cut(s) 213, 353
HapII CCGG 1 cut(s) 66
HhaI GCGC 3 cut(s) 212, 236, 352
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 3 cut(s) 210, 234, 350
HinP1I GCGC 3 cut(s) 210, 234, 350
HpaII CCGG 1 cut(s) 66
HphI GGTGA 2 cut(s) 323, 329
Hpy188III TCNNGA 3 cut(s) 195, 216, 447
Hpy99I CGWCG 4 cut(s) 278, 281, 284, 308
HpyAV CCTTC 2 cut(s) 127, 144
HpyCH4III ACNGT 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 3 cut(s) 210, 234, 350
KasI GGCGCC 1 cut(s) 209
KpnI GGTACC 1 cut(s) 363
Kzo9I GATC 1 cut(s) 429
LguI GCTCTTC 1 cut(s) 260
LpnPI CCDG 2 cut(s) 79, 398
Lsp1109I GCAGC 1 cut(s) 218
MaeI CTAG 2 cut(s) 206, 216
MalI GATC 1 cut(s) 431
MboI GATC 1 cut(s) 429
MboII GAAGA 3 cut(s) 136, 231, 247
MluCI AATT 1 cut(s) 28
Mly113I GGCGCC 1 cut(s) 210
MnlI CCTC 9 cut(s) 118, 133, 136, 152, 184, 223, 254, 302, 325
MseI TTAA 2 cut(s) 354, 437
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 240
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 1 cut(s) 66
NdeII GATC 1 cut(s) 429
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 3 cut(s) 87, 211, 361
PciSI GCTCTTC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 184
PkrI GCNGC 3 cut(s) 233, 238, 411
PluTI GGCGCC 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 412
PspN4I GGNNCC 3 cut(s) 87, 211, 361
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 354, 437
SatI GCNGC 3 cut(s) 232, 237, 410
Sau3AI GATC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 66
SetI ASST 3 cut(s) 110, 313, 361
SfoI GGCGCC 1 cut(s) 211
SmlI CTYRAG 3 cut(s) 14, 193, 245
SmoI CTYRAG 3 cut(s) 14, 193, 245
Sse9I AATT 1 cut(s) 28
SsiI CCGC 5 cut(s) 111, 162, 237, 371, 409
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 2 cut(s) 206, 216
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 432
TasI AATT 1 cut(s) 28
TauI GCSGC 2 cut(s) 239, 412
Tru1I TTAA 2 cut(s) 354, 437
Tru9I TTAA 2 cut(s) 354, 437
TseI GCWGC 1 cut(s) 231
TspDTI ATGAA 2 cut(s) 11, 126
TspGWI ACGGA 2 cut(s) 87, 321
XbaI TCTAGA 1 cut(s) 215
XspI CTAG 2 cut(s) 206, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.