RLG00000025737

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0020486
Physical Location & Seq
Reverse (-)
47961293 .. 47965690
4398 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025737

Sequence Viewer

Length: 582 bp
ATGAAAAGATCAATTGAATTGCAGTTGAGGGAGGCTAGAGATGAAGAACACAGATGCAAGGGGAGGAAGAACACAGATGTAATGGAGGTCGGAGATGGCCGAATATTGCCGGATTTTAGCCGACGAGTTCTTGACTTCTTGCCGGAAACAAAGATAGCAGAGGTCGAATTCACCATCTGCATATCGACGGAGATGCATCAGATGTTGGAGAAATTAGTGGAGAACTACCAGAAAGAAAGCCTCAAGTGGCTCTTCAATAATCCCAAGAGTGACGGCGGCAGCGAGATTGAAAAAATCTGCGGCAGAGGTGATGATGATGATGAGGGCGAGAGTGGGTTGGCGCTTAAAGGTATTGTCTTGGTGGGGCTGTTCTTGGTTGGGATTGTTGGTGGATTTGGGGCGGCTGGGTATTTGTACAAGGATCAGGTTAATGCCTTCTTGAATCAGTTTTTGACTCTCATTGAAGTTTTTGATCGAGTTGATTTTGATTGTGGCGGTGTGTTCAGCGTCGTGCAATTTCTATTTGCTAGCTACATGTTAGATGGTGATGATGAATCAATGATGATCATGATGGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

194

Amino Acids

21.8

Weight (kDa)

4.51

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020486)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0376751
rosa_laevigata RLG00000002423 RLG00000025737
rosa_roxburghii Rroxscaffold_3G00237280
rosa_samantha Rh4BG153200 Rh5BG374400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 276, 300, 401, 495
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 5 cut(s) 17, 256, 290, 442, 464
AluBI AGCT 1 cut(s) 531
AluI AGCT 1 cut(s) 531
AlwI GGATC 1 cut(s) 429
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 279
ApoI RAATTY 1 cut(s) 167
AspLEI GCGC 1 cut(s) 343
AsuHPI GGTGA 3 cut(s) 163, 320, 557
AsuNHI GCTAGC 1 cut(s) 527
BbvI GCAGC 1 cut(s) 291
BccI CCATC 4 cut(s) 89, 182, 536, 565
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BclI TGATCA 1 cut(s) 564
BfaI CTAG 2 cut(s) 36, 528
BfoI RGCGCY 1 cut(s) 344
BisI GCNGC 4 cut(s) 277, 280, 301, 402
BlsI GCNGC 4 cut(s) 278, 281, 302, 403
BmsI GCATC 3 cut(s) 44, 183, 205
BmtI GCTAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 227
BseGI GGATG 1 cut(s) 580
BseXI GCAGC 1 cut(s) 291
BseYI CCCAGC 1 cut(s) 404
BshFI GGCC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 110, 143
BsnI GGCC 1 cut(s) 99
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 4 cut(s) 8, 421, 472, 564
BspACI CCGC 4 cut(s) 276, 300, 401, 495
BspANI GGCC 1 cut(s) 99
BspHI TCATGA 1 cut(s) 567
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 1 cut(s) 429
BspQI GCTCTTC 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 414
BssMI GATC 4 cut(s) 8, 421, 472, 564
Bst6I CTCTTC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 1 cut(s) 529
BstF5I GGATG 1 cut(s) 580
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 11, 424, 475, 567
BstMBI GATC 4 cut(s) 8, 421, 472, 564
BstNSI RCATGY 1 cut(s) 538
BstV1I GCAGC 1 cut(s) 291
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 529
CciI TCATGA 1 cut(s) 567
CfoI GCGC 1 cut(s) 343
CseI GACGC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 415
CviAII CATG 2 cut(s) 535, 568
CviJI RGCY 8 cut(s) 35, 99, 120, 240, 250, 367, 404, 531
CviKI_1 RGCY 8 cut(s) 35, 99, 120, 240, 250, 367, 404, 531
CviQI GTAC 1 cut(s) 415
DpnI GATC 4 cut(s) 10, 423, 474, 566
DpnII GATC 4 cut(s) 8, 421, 472, 564
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 167
EcoT22I ATGCAT 1 cut(s) 198
FaeI CATG 2 cut(s) 538, 571
FaiI YATR 3 cut(s) 182, 536, 569
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FatI CATG 2 cut(s) 534, 567
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 277, 280, 301, 402
Fsp4HI GCNGC 4 cut(s) 277, 280, 301, 402
FspBI CTAG 2 cut(s) 36, 528
GlaI GCGC 1 cut(s) 342
GluI GCNGC 4 cut(s) 277, 280, 301, 402
GsaI CCCAGC 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 1 cut(s) 99
HapII CCGG 2 cut(s) 110, 143
HgaI GACGC 1 cut(s) 496
HhaI GCGC 1 cut(s) 343
Hin1II CATG 2 cut(s) 538, 571
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 3 cut(s) 442, 454, 554
HpaII CCGG 2 cut(s) 110, 143
HphI GGTGA 3 cut(s) 163, 320, 557
Hpy188I TCNGA 2 cut(s) 92, 201
Hpy188III TCNNGA 3 cut(s) 131, 439, 568
Hpy99I CGWCG 3 cut(s) 126, 190, 512
HpyAV CCTTC 1 cut(s) 445
HpyCH4V TGCA 5 cut(s) 22, 57, 180, 196, 514
Hsp92II CATG 2 cut(s) 538, 571
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 4 cut(s) 8, 421, 472, 564
LguI GCTCTTC 1 cut(s) 257
LpnPI CCDG 5 cut(s) 123, 156, 242, 390, 410
Lsp1109I GCAGC 1 cut(s) 291
LweI GCATC 3 cut(s) 44, 183, 205
MaeI CTAG 2 cut(s) 36, 528
MaeIII GTNAC 1 cut(s) 269
MalI GATC 4 cut(s) 10, 423, 474, 566
MboI GATC 4 cut(s) 8, 421, 472, 564
MboII GAAGA 3 cut(s) 56, 79, 244
MfeI CAATTG 1 cut(s) 12
MluCI AATT 5 cut(s) 12, 17, 167, 212, 515
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 70, 186
MnlI CCTC 8 cut(s) 21, 25, 57, 79, 154, 251, 299, 316
Mph1103I ATGCAT 1 cut(s) 198
MseI TTAA 2 cut(s) 345, 429
MspI CCGG 2 cut(s) 110, 143
MunI CAATTG 1 cut(s) 12
NdeII GATC 4 cut(s) 8, 421, 472, 564
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 2 cut(s) 538, 571
NmuCI GTSAC 1 cut(s) 269
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 1 cut(s) 538
PagI TCATGA 1 cut(s) 567
PciI ACATGT 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 2 cut(s) 442, 554
PkrI GCNGC 4 cut(s) 278, 281, 302, 403
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 534
PspFI CCCAGC 1 cut(s) 404
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 345, 429
SatI GCNGC 4 cut(s) 277, 280, 301, 402
Sau3AI GATC 4 cut(s) 8, 421, 472, 564
SchI GAGTC 1 cut(s) 448
SetI ASST 6 cut(s) 90, 165, 310, 352, 429, 533
SfaNI GCATC 3 cut(s) 44, 183, 205
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 5 cut(s) 12, 17, 167, 212, 515
SsiI CCGC 4 cut(s) 276, 300, 401, 495
SspI AATATT 1 cut(s) 105
SspMI CTAG 2 cut(s) 36, 528
TaqI TCGA 3 cut(s) 165, 185, 475
TasI AATT 5 cut(s) 12, 17, 167, 212, 515
TatI WGTACW 1 cut(s) 414
TauI GCSGC 3 cut(s) 279, 303, 404
TfiI GAWTC 2 cut(s) 442, 554
Tru1I TTAA 2 cut(s) 345, 429
Tru9I TTAA 2 cut(s) 345, 429
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 279
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 3 cut(s) 17, 57, 567
TspGWI ACGGA 1 cut(s) 203
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 538
XspI CTAG 2 cut(s) 36, 528
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.