Rh4BG153200

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0020486
Physical Location & Seq
Forward (+)
27594238 .. 27599661
5424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG153200.1

Sequence Viewer

Length: 408 bp
ATGAAGCTTGTTGAAGCAGAAAGGGGATTTGGGTCTGGGATGAATCATGGTAGTTGGAGGAGAAATGATGAAGAAGAAGAGGTGGCCAAGAGCCTCAAGTGGCTCTTCAATAATCCCAAGAGCGACGACGGCGGCAAGATTGAAAAAATCGGCGGCGGAGGTGATGATGATGATGAGGGCGAAAGTGGGTTAACGCTTAAAGGTATTGTCTTGGCGGGGCTGTTCTTGGTTAGGATTATTGGTGGGTTTGGGGCGGCTGGGTATTTGTACAAGGATCAGAGACCTGAGCTTCAACAGCTTAACCGGTCCTTTTCAAATAATATGCTTACTGGGGAAGTTCCTAATTGGATTCAAAATGCAGTTTGGAGTAAGATGTATGTTTTAAGAAAAAACATGTTTTCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

135

Amino Acids

15.02

Weight (kDa)

5.47

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020486)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0376751
rosa_laevigata RLG00000002423 RLG00000025737
rosa_roxburghii Rroxscaffold_3G00237280
rosa_samantha Rh4BG153200 Rh5BG374400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 132, 153, 156, 215, 254
AclWI GGATC 1 cut(s) 282
AcoI YGGCCR 1 cut(s) 84
AfaI GTAC 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 393
AgeI ACCGGT 1 cut(s) 303
AgsI TTSAA 6 cut(s) 14, 109, 143, 293, 315, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AluBI AGCT 3 cut(s) 7, 289, 298
AluI AGCT 3 cut(s) 7, 289, 298
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 1 cut(s) 282
AoxI GGCC 1 cut(s) 84
AsiGI ACCGGT 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 173
AvaII GGWCC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 86
BceAI ACGGC 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 1 cut(s) 402
BisI GCNGC 3 cut(s) 133, 154, 255
BlsI GCNGC 3 cut(s) 134, 155, 256
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 306
BmrI ACTGGG 1 cut(s) 339
BmuI ACTGGG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 274
BsaWI WCCGGW 1 cut(s) 303
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 1 cut(s) 334
BseGI GGATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 257
BshFI GGCC 1 cut(s) 86
BshTI ACCGGT 1 cut(s) 303
BsiSI CCGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 274
BsnI GGCC 1 cut(s) 86
Bso31I GGTCTC 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 274
BspACI CCGC 5 cut(s) 132, 153, 156, 215, 254
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 282
BspQI GCTCTTC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 303
BssMI GATC 1 cut(s) 274
Bst6I CTCTTC 2 cut(s) 72, 110
BstAUI TGTACA 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 2 cut(s) 129, 295
BstNSI RCATGY 1 cut(s) 397
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 306
Csp6I GTAC 1 cut(s) 268
CspAI ACCGGT 1 cut(s) 303
CviAII CATG 2 cut(s) 47, 394
CviJI RGCY 8 cut(s) 7, 86, 93, 103, 220, 257, 289, 298
CviKI_1 RGCY 8 cut(s) 7, 86, 93, 103, 220, 257, 289, 298
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 1 cut(s) 285
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 72, 110
EarI CTCTTC 2 cut(s) 72, 110
EciI GGCGGA 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 306
FaeI CATG 2 cut(s) 50, 397
FaiI YATR 4 cut(s) 48, 323, 378, 395
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 2 cut(s) 46, 393
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 133, 154, 255
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 3 cut(s) 133, 154, 255
FspBI CTAG 1 cut(s) 402
GluI GCNGC 3 cut(s) 133, 154, 255
GsaI CCCAGC 1 cut(s) 261
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 304
Hin1II CATG 2 cut(s) 50, 397
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 43, 349
HpaI GTTAAC 1 cut(s) 192
HpaII CCGG 1 cut(s) 304
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 1 cut(s) 279
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 2 cut(s) 128, 131
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 295
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 2 cut(s) 50, 397
KspAI GTTAAC 1 cut(s) 192
Kzo9I GATC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 110
LpnPI CCDG 5 cut(s) 21, 243, 297, 315, 317
MaeI CTAG 1 cut(s) 402
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 4 cut(s) 83, 86, 89, 97
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 35
MnlI CCTC 5 cut(s) 51, 73, 104, 152, 169
Mox20I TGGCCA 1 cut(s) 86
MscI TGGCCA 1 cut(s) 86
MseI TTAA 5 cut(s) 191, 198, 300, 383, 406
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 129, 295
NdeII GATC 1 cut(s) 274
NlaIII CATG 2 cut(s) 50, 397
NspI RCATGY 1 cut(s) 397
PciI ACATGT 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 43, 349
PinAI ACCGGT 1 cut(s) 303
PkrI GCNGC 3 cut(s) 134, 155, 256
PscI ACATGT 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 257
PspPI GGNCC 1 cut(s) 306
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 5 cut(s) 191, 198, 300, 383, 406
SatI GCNGC 3 cut(s) 133, 154, 255
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 306
SetI ASST 7 cut(s) 9, 84, 163, 205, 286, 291, 300
SinI GGWCC 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 343
SsiI CCGC 5 cut(s) 132, 153, 156, 215, 254
SspMI CTAG 1 cut(s) 402
TasI AATT 1 cut(s) 343
TatI WGTACW 1 cut(s) 267
TauI GCSGC 3 cut(s) 135, 156, 257
TfiI GAWTC 2 cut(s) 43, 349
Tru1I TTAA 5 cut(s) 191, 198, 300, 383, 406
Tru9I TTAA 5 cut(s) 191, 198, 300, 383, 406
TspDTI ATGAA 3 cut(s) 17, 56, 84
VpaK11BI GGWCC 1 cut(s) 306
XceI RCATGY 1 cut(s) 397
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.