RLG00000003182
MADS Family

MADS-box transcription factor PHERES 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018045
Physical Location & Seq
Reverse (-)
45351059 .. 45351985
927 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003182

Sequence Viewer

Length: 927 bp
ATGCCTAGGGCTAGGGTCAAACTTGCATGGATAGGGAGTGAGAGTGCTAGGAGAGCAAGCTTCAACAAGAGGAAAGACGGTCTTGTGAAGAAAGTGAGCGAGCTTAGCACTTTGTGCGATGTGCCTGCTTGTATCATTATCTATGGTCGGGGCAGTAACCAGCCGATTGTATGGCCTGACCGCCCAAGAGCAGAGGAAGTGATCCGCAGATACCTGAGCCTACCGGGTTATGTGCGGTGCAAGAAGACGCTGAGCCAAGAGACTTACCTCAAGGAGAAGGTCGCCAAAATGGAAGAGAAGCACAACAAAACTCTCAGATCCAACACTGTGAAAGACATGGATGATCTCATGCGCCAAGGTCAGAATGGTAAGCCGTTATATGAACTTGACATTAATCAAACATATGGTCTGCTTAACTTTGTGGAGGAGAAGATGAAAGAGATCCAGAACCAGTTCTCTTTCTCTGAGCAAGCTGGTGGTCCTAGTTACACCAGTGTCCACAATGAACCCGCCAATAACTTGGGGGTGCCCCTGGGGACTGGTATGGAGCGGCCATATGGAATTTTTGGAGTAGGAGGTGATGGAAGTGGTAGTGGAACTGATATGGGGTTTCCGATGTTGCCTCAATGTTATGGGAATAATATTGGAGGTTGCAGCGGTGTTAATGTGGCGCCCAATATGACAACATACAATGGTAACATTGGAAATTTTGGAGGTGATGGAGATGGTAGTGTTGGGTTACCTCAGGTGAATTATTTAGGCAATGATATTACTGGGAATGGAAGTCAGATGGGGTACTTCGGGATGCTGCCTCAATATTATGGGGCTAATGTGATAACATCAAATGGTAGTATTATTGGATGGGCAATACGAAATACCAACGGAGCCAACAGTGCCGCTGAAGGTGATGCTGCAGGCCTTCCTTAA

Protein Analysis

309

Amino Acids

33.26

Weight (kDa)

8.17

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 11 - 50 3.1e-15 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018045)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21500
rosa_chinensis RchiOBHm_Chr7g0208901
rosa_laevigata RLG00000003182
rosa_multiflora Rmu_sc0000532.1_g000022
rosa_roxburghii Rroxscaffold_3G00250040
rosa_rugosa Rorug07G0107300
rosa_samantha Rh7AG242400 Rh7BG237200 Rh7CG257300 Rh7DG248400
rosa_wichuraiana Rw7G020520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 526, 670
AccBSI CCGCTC 1 cut(s) 550
AciI CCGC 7 cut(s) 181, 205, 235, 510, 550, 657, 897
AclWI GGATC 3 cut(s) 196, 312, 436
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 2 cut(s) 561, 706
AcuI CTGAAG 1 cut(s) 921
AcyI GRCGYC 1 cut(s) 671
AdeI CACNNNGTG 1 cut(s) 114
AfaI GTAC 1 cut(s) 797
AgsI TTSAA 1 cut(s) 64
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 3 cut(s) 60, 103, 473
AluI AGCT 3 cut(s) 60, 103, 473
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 3 cut(s) 196, 312, 436
AoxI GGCC 3 cut(s) 173, 551, 916
ApeKI GCWGC 3 cut(s) 654, 808, 911
ApoI RAATTY 2 cut(s) 561, 706
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 452
AspA2I CCTAGG 1 cut(s) 5
AspLEI GCGC 2 cut(s) 354, 673
AspS9I GGNCC 1 cut(s) 479
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 4 cut(s) 590, 728, 760, 917
AvaII GGWCC 1 cut(s) 479
AvrII CCTAGG 1 cut(s) 5
AxyI CCTNAGG 1 cut(s) 744
BaeGI GKGCMC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 526, 670
BbsI GAAGAC 1 cut(s) 251
BbvI GCAGC 3 cut(s) 666, 795, 898
BccI CCATC 5 cut(s) 575, 713, 719, 784, 855
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 1 cut(s) 533
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 4 cut(s) 6, 12, 48, 483
BfmI CTRYAG 1 cut(s) 912
BfoI RGCGCY 1 cut(s) 674
BisI GCNGC 5 cut(s) 551, 655, 809, 897, 912
BlnI CCTAGG 1 cut(s) 5
BlpI GCTNAGC 2 cut(s) 104, 251
BlsI GCNGC 5 cut(s) 552, 656, 810, 898, 913
Bme1390I CCNGG 2 cut(s) 225, 533
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 3 cut(s) 528, 672, 886
BmrFI CCNGG 2 cut(s) 225, 533
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 2 cut(s) 795, 898
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 215
Bpu1102I GCTNAGC 2 cut(s) 104, 251
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 671
BsaJI CCNNGG 4 cut(s) 5, 355, 531, 532
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bse1I ACTGG 4 cut(s) 451, 492, 544, 778
Bse21I CCTNAGG 1 cut(s) 744
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 4 cut(s) 5, 355, 531, 532
BseGI GGATG 3 cut(s) 346, 810, 866
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 5 cut(s) 206, 242, 328, 456, 758
BseNI ACTGG 4 cut(s) 451, 492, 544, 778
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 3 cut(s) 666, 795, 898
BshFI GGCC 3 cut(s) 175, 553, 918
BshNI GGYRCC 2 cut(s) 526, 670
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 3 cut(s) 175, 553, 918
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 4 cut(s) 201, 317, 343, 441
Bsp1720I GCTNAGC 2 cut(s) 104, 251
BspACI CCGC 7 cut(s) 181, 205, 235, 510, 550, 657, 897
BspANI GGCC 3 cut(s) 175, 553, 918
BspCNI CTCAG 5 cut(s) 207, 243, 327, 457, 757
BspLI GGNNCC 3 cut(s) 528, 672, 886
BspMAI CTGCAG 1 cut(s) 916
BspPI GGATC 3 cut(s) 196, 312, 436
BspT107I GGYRCC 2 cut(s) 526, 670
BsrBI CCGCTC 1 cut(s) 550
BsrDI GCAATG 1 cut(s) 769
BsrI ACTGG 4 cut(s) 451, 492, 544, 778
BssECI CCNNGG 4 cut(s) 5, 355, 531, 532
BssMI GATC 4 cut(s) 201, 317, 343, 441
BssNI GRCGYC 1 cut(s) 671
BssT1I CCWWGG 2 cut(s) 5, 355
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 80, 328, 893
Bst6I CTCTTC 1 cut(s) 288
BstACI GRCGYC 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 114
BstC8I GCNNGC 5 cut(s) 58, 101, 126, 471, 916
BstDEI CTNAG 6 cut(s) 104, 215, 251, 314, 465, 744
BstEII GGTNACC 1 cut(s) 738
BstF5I GGATG 3 cut(s) 346, 810, 866
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 2 cut(s) 354, 673
BstKTI GATC 4 cut(s) 204, 320, 346, 444
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 4 cut(s) 201, 317, 343, 441
BstMWI GCNNNNNNNGC 4 cut(s) 53, 105, 114, 893
BstNI CCWGG 1 cut(s) 533
BstPI GGTNACC 1 cut(s) 738
BstSCI CCNGG 2 cut(s) 223, 531
BstSFI CTRYAG 1 cut(s) 912
BstSLI GKGCMC 1 cut(s) 531
BstV1I GCAGC 3 cut(s) 666, 795, 898
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 317, 441
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 2 cut(s) 317, 441
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 3 cut(s) 175, 553, 918
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 3 cut(s) 346, 810, 866
BtsIMutI CAGTG 3 cut(s) 324, 499, 898
Cac8I GCNNGC 5 cut(s) 58, 101, 126, 471, 916
CfoI GCGC 2 cut(s) 354, 673
Cfr13I GGNCC 1 cut(s) 479
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 796
CviAII CATG 3 cut(s) 27, 337, 349
CviQI GTAC 1 cut(s) 796
DdeI CTNAG 6 cut(s) 104, 215, 251, 314, 465, 744
DinI GGCGCC 1 cut(s) 672
DpnI GATC 4 cut(s) 203, 319, 345, 443
DpnII GATC 4 cut(s) 201, 317, 343, 441
DraIII CACNNNGTG 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 551
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 5, 355
Eco147I AGGCCT 1 cut(s) 918
Eco47I GGWCC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 921
Eco81I CCTNAGG 1 cut(s) 744
Eco91I GGTNACC 1 cut(s) 738
EcoO65I GGTNACC 1 cut(s) 738
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 2 cut(s) 5, 355
EgeI GGCGCC 1 cut(s) 672
EheI GGCGCC 1 cut(s) 672
ErhI CCWWGG 2 cut(s) 5, 355
FaeI CATG 3 cut(s) 30, 340, 352
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 1 cut(s) 550
FatI CATG 3 cut(s) 26, 336, 348
FauI CCCGC 1 cut(s) 517
FauNDI CATATG 2 cut(s) 403, 556
Fnu4HI GCNGC 5 cut(s) 551, 655, 809, 897, 912
FokI GGATG 3 cut(s) 353, 817, 873
Fsp4HI GCNGC 5 cut(s) 551, 655, 809, 897, 912
FspBI CTAG 4 cut(s) 6, 12, 48, 483
GlaI GCGC 2 cut(s) 353, 672
GluI GCNGC 5 cut(s) 551, 655, 809, 897, 912
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 3 cut(s) 175, 553, 918
HapII CCGG 1 cut(s) 224
HgaI GACGC 1 cut(s) 256
HhaI GCGC 2 cut(s) 354, 673
Hin1I GRCGYC 1 cut(s) 671
Hin1II CATG 3 cut(s) 30, 340, 352
Hin6I GCGC 2 cut(s) 352, 671
HinP1I GCGC 2 cut(s) 352, 671
HindIII AAGCTT 1 cut(s) 58
HpaII CCGG 1 cut(s) 224
HphI GGTGA 4 cut(s) 590, 728, 760, 917
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 5 cut(s) 317, 363, 466, 615, 789
Hpy188III TCNNGA 2 cut(s) 445, 802
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 2 cut(s) 271, 896
HpyCH4III ACNGT 3 cut(s) 80, 328, 893
HpyCH4V TGCA 4 cut(s) 26, 240, 654, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 105, 114, 893
HpyF3I CTNAG 6 cut(s) 104, 215, 251, 314, 465, 744
Hsp92I GRCGYC 1 cut(s) 671
Hsp92II CATG 3 cut(s) 30, 340, 352
HspAI GCGC 2 cut(s) 352, 671
KasI GGCGCC 1 cut(s) 670
Kzo9I GATC 4 cut(s) 201, 317, 343, 441
LmnI GCTCC 2 cut(s) 547, 884
Lsp1109I GCAGC 3 cut(s) 666, 795, 898
LweI GCATC 2 cut(s) 795, 898
MaeI CTAG 4 cut(s) 6, 12, 48, 483
MaeIII GTNAC 4 cut(s) 155, 485, 695, 738
MalI GATC 4 cut(s) 203, 319, 345, 443
MbiI CCGCTC 1 cut(s) 550
MboI GATC 4 cut(s) 201, 317, 343, 441
MboII GAAGA 4 cut(s) 100, 256, 305, 442
MflI RGATCY 2 cut(s) 317, 441
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 3 cut(s) 561, 706, 751
Mly113I GGCGCC 1 cut(s) 671
MmeI TCCRAC 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 4 cut(s) 393, 414, 663, 925
MspA1I CMGCKG 2 cut(s) 657, 899
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 225, 533
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 4 cut(s) 53, 105, 114, 893
NarI GGCGCC 1 cut(s) 671
NciI CCSGG 1 cut(s) 225
NdeI CATATG 2 cut(s) 403, 556
NdeII GATC 4 cut(s) 201, 317, 343, 441
NlaIII CATG 3 cut(s) 30, 340, 352
NlaIV GGNNCC 3 cut(s) 528, 672, 886
PasI CCCWGGG 1 cut(s) 532
PceI AGGCCT 1 cut(s) 918
PdmI GAANNNNTTC 1 cut(s) 452
PkrI GCNGC 5 cut(s) 552, 656, 810, 898, 913
PluTI GGCGCC 1 cut(s) 674
PshBI ATTAAT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 531
PspEI GGTNACC 1 cut(s) 738
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 528, 672, 886
PspPI GGNCC 1 cut(s) 479
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 2 cut(s) 317, 441
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
SaqAI TTAA 4 cut(s) 393, 414, 663, 925
SatI GCNGC 5 cut(s) 551, 655, 809, 897, 912
Sau3AI GATC 4 cut(s) 201, 317, 343, 441
Sau96I GGNCC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 225, 533
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 2 cut(s) 795, 898
SfcI CTRYAG 1 cut(s) 912
SfoI GGCGCC 1 cut(s) 672
SinI GGWCC 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 3 cut(s) 561, 706, 751
SseBI AGGCCT 1 cut(s) 918
SsiI CCGC 7 cut(s) 181, 205, 235, 510, 550, 657, 897
SspDI GGCGCC 1 cut(s) 670
SspI AATATT 2 cut(s) 643, 818
SspMI CTAG 4 cut(s) 6, 12, 48, 483
StuI AGGCCT 1 cut(s) 918
StyD4I CCNGG 2 cut(s) 223, 531
StyI CCWWGG 2 cut(s) 5, 355
TaaI ACNGT 3 cut(s) 80, 328, 893
TasI AATT 3 cut(s) 561, 706, 751
TauI GCSGC 2 cut(s) 553, 899
Tru1I TTAA 4 cut(s) 393, 414, 663, 925
Tru9I TTAA 4 cut(s) 393, 414, 663, 925
TscAI CASTG 3 cut(s) 331, 499, 898
TseI GCWGC 3 cut(s) 654, 808, 911
TspDTI ATGAA 3 cut(s) 396, 449, 519
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 3 cut(s) 331, 499, 898
VpaK11BI GGWCC 1 cut(s) 479
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 2 cut(s) 561, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 4 cut(s) 6, 12, 48, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.