RLG00000004790

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019807
Physical Location & Seq
Forward (+)
62295749 .. 62302716
6968 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004790

Sequence Viewer

Length: 1740 bp
ATGCTTGACAGGTATGAATATCTATTTATACAAAAGAATGTTGAAGAGATATCATCCCAAACATTAAAGTGTACCTATTTGGATGCAGCCAAATATCCCGTTGGAATAGATTCTTGTTTAGACGAGATAGAGGAGCTTTTAGGGCTCGAAGAAAATGATATTCGCATGGTTGGGATCCGGGGCATTGGTGGTATAGGTAAAACCACACTTGCTAAAGCTGTTTATAATTCTTTTGCTCACAGCTTTGAAGGTTGTTGTTTTCTAGCAAATGTCAGAGAAAATTCAATGTCAGATGAAGGGTTAGTCCGACTACAGAAGACTCTCCTCTTTGATATTCTAAGAGACGCAAACTTGAATGTGACCAACGTTGTAGAGTATGCTCAAGGGCTTCCCCTAGCTGTGACAGTTTTAGGTTCTAATCTTTTTGGTAAAAGGATACGTGAATGGCAAGATACATTAGATTGTTATAAAAGAGTTCCCAACCAACAAATTCAAGAAATTCTTAGAATTAGTTACGATGCTTTAGAGGATCCTGTGAAAGAATTATTGCTTGACATTGCATGTTTCTTTAAAGGTGAAAGCATGACTCATGTTAGACAAATATTACAAGGTTGTGTGAGTGGCATTCGCGTACTGAAAGAAAAATCCCTCGTAACTATTGGCCAAAATAATAGGATTCTCATGCATGATCTGCTAGAGAAAATGGGGAAAGAAATAGTACGGCAGGAATCTCCGGTTGAACCTGGCAGACGTAGTAGATTGTGGCTTCCTCAAGACGTTTGTCATGTCTCACAGAATTTGAAGACTATGGATTTGAAAGGAAGCAAATTCCTATCAAAAAATCCCCGACTTATCTGGAATGCCAAATTTACAGACACTGAACCTAGATTATTGTACAAAGTTGAGGTTCATTATTCTGTTGGACGCCTTGAGAAACTAGTTACCTTGAGTCTTGAAGAATGCTGTAGACTCCAGATATTTCCAGGAAAAGTCAGCTTGAGTTCTCTGGAATCTATTAATCTTTCAGGTTGCTTTAAGCTCGAGTATTTCCCAGAAATAGAAGGAAAGATGGAATCCTTAAGAAGTATAGATCTAACTCTCACTTCCATCAAAGAACTGCCATCGTCAATCGGCTGCCTCCTCGGGCTTCGTGAGTTGTACTTGAATGGAGACCACTGCAGCCAGGATTGGTACAGTGATTGCAATTGCTATTTCCGAGCTGTAGTTCACATCAATGGCGTGCACATCGGTGATTATCAGTCCCCCTTCTATCCTCCTCGAACGACGCGACAGGAACATATATGGCTGCAATATGATCCATTGCCAGTTGAGTTGAAGGCCAAGGTAGATGGAGAGACTGGGTTGCTGCGTTTGGGTACTCATTACAACTCTCGGGTTGAGTTTTACTTCTGGCTGGGTACAGCTGAAAGAAAGTCATGCATAAAGCTACACATTTTGAAATGCGGAGCACTCATAGTGGGTGGGGATGAGATTCCTGGTCTTGATTTTTGGTGGGAACGGATCCGGAACGGTGGCTCCCCATTCCAATCCAAAGTCTTGCAGAATCAAATATCAACTGGGACACTACGGAAATTAATCCGAATAGGGATGTCACTGAGTTTCTGCATTATCCGATTCGCAATGTCACTGATATTCAGCATGTCCATAGTCTTTATTGCTTCAGTCCTTTGGAGCCAGATCCAGAATTCTGACATGCTGCAAACTTATCCAGAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

580

Amino Acids

66.01

Weight (kDa)

6.45

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 41 - 89 2.3e-06 NB-ARC domain
WHD_ROQ1 PF23282 180 - 244 3.4e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 333 - 384 8.5e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019807)

Species Orthologous Gene IDs
prunus_persica Prupe.1G018600_v2.0.a1
rosa_laevigata RLG00000004790
rosa_multiflora Rmu_sc0001758.1_g000009 Rmu_sc0029164.1_g000001 Rmu_sc0029165.1_g000001
rosa_roxburghii Rroxscaffold_3G00267550
rosa_samantha Rh7CG086500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 225, 468
AccI GTMKAC 1 cut(s) 967
AccII CGCG 2 cut(s) 630, 1288
AccIII TCCGGA 1 cut(s) 1524
AciI CCGC 1 cut(s) 1464
AclI AACGTT 1 cut(s) 366
AclWI GGATC 8 cut(s) 169, 182, 524, 537, 1310, 1516, 1529, 1693
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 7 cut(s) 280, 489, 498, 796, 827, 866, 1705
AcuI CTGAAG 1 cut(s) 1665
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 8 cut(s) 73, 633, 720, 896, 1160, 1193, 1378, 1420
AflII CTTAAG 1 cut(s) 1078
AhlI ACTAGT 1 cut(s) 937
AjnI CCWGG 4 cut(s) 742, 982, 1182, 1495
AleI CACNNNNGTG 1 cut(s) 1248
AloI GAACNNNNNNTCC 2 cut(s) 1520, 1552
AluBI AGCT 9 cut(s) 136, 218, 243, 398, 996, 1039, 1220, 1424, 1447
AluI AGCT 9 cut(s) 136, 218, 243, 398, 996, 1039, 1220, 1424, 1447
Alw21I GWGCWC 2 cut(s) 1245, 1471
Alw26I GTCTC 4 cut(s) 336, 793, 1164, 1349
Alw44I GTGCAC 1 cut(s) 1241
AlwI GGATC 8 cut(s) 169, 182, 524, 537, 1310, 1516, 1529, 1693
AlwNI CAGNNNCTG 1 cut(s) 878
Ama87I CYCGRG 3 cut(s) 1040, 1142, 1392
Aor13HI TCCGGA 1 cut(s) 1524
AoxI GGCC 2 cut(s) 661, 1338
ApaLI GTGCAC 1 cut(s) 1241
ApeKI GCWGC 6 cut(s) 86, 1134, 1179, 1306, 1366, 1717
ApoI RAATTY 7 cut(s) 280, 489, 498, 796, 827, 866, 1705
ArsI GACNNNNNNTTYG 2 cut(s) 796, 828
AseI ATTAAT 2 cut(s) 1017, 1595
Asp700I GAANNNNTTC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 587, 1262
AvaI CYCGRG 3 cut(s) 1040, 1142, 1392
BaeGI GKGCMC 1 cut(s) 1245
BalI TGGCCA 1 cut(s) 663
BamHI GGATCC 3 cut(s) 174, 529, 1521
BanII GRGCYC 1 cut(s) 147
BbsI GAAGAC 2 cut(s) 323, 809
Bbv12I GWGCWC 2 cut(s) 1245, 1471
BbvI GCAGC 6 cut(s) 98, 1121, 1191, 1293, 1353, 1704
BccI CCATC 4 cut(s) 1063, 1115, 1129, 1343
BceAI ACGGC 1 cut(s) 737
BciT130I CCWGG 4 cut(s) 744, 984, 1184, 1497
BciVI GTATCC 1 cut(s) 429
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 4 cut(s) 336, 793, 1164, 1349
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 5 cut(s) 263, 395, 695, 885, 938
BfmI CTRYAG 4 cut(s) 311, 964, 1177, 1221
BfrI CTTAAG 1 cut(s) 1078
BfuI GTATCC 1 cut(s) 429
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 6 cut(s) 87, 1135, 1180, 1307, 1367, 1718
BlsI GCNGC 6 cut(s) 88, 1136, 1181, 1308, 1368, 1719
Bme1390I CCNGG 5 cut(s) 179, 744, 984, 1184, 1497
BmeT110I CYCGRG 3 cut(s) 1040, 1142, 1392
BmiI GGNNCC 5 cut(s) 176, 531, 1523, 1537, 1694
BmrFI CCNGG 5 cut(s) 179, 744, 984, 1184, 1497
BmrI ACTGGG 2 cut(s) 1368, 1587
BmsI GCATC 2 cut(s) 73, 508
BmuI ACTGGG 2 cut(s) 1368, 1587
BoxI GACNNNNGTC 1 cut(s) 780
BpiI GAAGAC 2 cut(s) 323, 809
BpmI CTGGAG 1 cut(s) 956
BpuEI CTTGAG 5 cut(s) 366, 756, 950, 967, 1018
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 1164
BsaJI CCNNGG 3 cut(s) 178, 1141, 1341
BsaWI WCCGGW 2 cut(s) 733, 1524
BsaXI ACNNNNNCTCC 2 cut(s) 1520, 1550
Bse1I ACTGG 3 cut(s) 1325, 1363, 1582
Bse3DI GCAATG 3 cut(s) 555, 1319, 1647
BseAI TCCGGA 1 cut(s) 1524
BseBI CCWGG 4 cut(s) 744, 984, 1184, 1497
BseDI CCNNGG 3 cut(s) 178, 1141, 1341
BseGI GGATG 4 cut(s) 53, 88, 1492, 1614
BseMI GCAATG 3 cut(s) 555, 1319, 1647
BseMII CTCAG 1 cut(s) 1607
BseNI ACTGG 3 cut(s) 1325, 1363, 1582
BseRI GAGGAG 4 cut(s) 146, 314, 1130, 1266
BseSI GKGCMC 1 cut(s) 1245
BseXI GCAGC 6 cut(s) 98, 1121, 1191, 1293, 1353, 1704
BseYI CCCAGC 1 cut(s) 1414
Bsh1236I CGCG 2 cut(s) 630, 1288
BshFI GGCC 2 cut(s) 663, 1340
BsiHKAI GWGCWC 2 cut(s) 1245, 1471
BsiHKCI CYCGRG 3 cut(s) 1040, 1142, 1392
BsiSI CCGG 3 cut(s) 178, 734, 1525
BslFI GGGAC 2 cut(s) 1246, 1594
BsmAI GTCTC 4 cut(s) 336, 793, 1164, 1349
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 1246, 1594
BsmI GAATGC 3 cut(s) 624, 865, 965
BsnI GGCC 2 cut(s) 663, 1340
Bso31I GGTCTC 1 cut(s) 1164
BsoBI CYCGRG 3 cut(s) 1040, 1142, 1392
Bsp1286I GDGCHC 3 cut(s) 147, 1245, 1471
Bsp13I TCCGGA 1 cut(s) 1524
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
BspACI CCGC 1 cut(s) 1464
BspANI GGCC 2 cut(s) 663, 1340
BspCNI CTCAG 1 cut(s) 1608
BspEI TCCGGA 1 cut(s) 1524
BspFNI CGCG 2 cut(s) 630, 1288
BspLI GGNNCC 5 cut(s) 176, 531, 1523, 1537, 1694
BspMAI CTGCAG 1 cut(s) 1181
BspPI GGATC 8 cut(s) 169, 182, 524, 537, 1310, 1516, 1529, 1693
BspTI CTTAAG 1 cut(s) 1078
BspTNI GGTCTC 1 cut(s) 1164
BsrDI GCAATG 3 cut(s) 555, 1319, 1647
BsrGI TGTACA 1 cut(s) 894
BsrI ACTGG 3 cut(s) 1325, 1363, 1582
BssECI CCNNGG 3 cut(s) 178, 1141, 1341
BssMI GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
BssNI GRCGYC 1 cut(s) 925
BssT1I CCWWGG 1 cut(s) 1341
Bst2UI CCWGG 4 cut(s) 744, 984, 1184, 1497
Bst4CI ACNGT 3 cut(s) 406, 1196, 1532
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 925
BstAFI CTTAAG 1 cut(s) 1078
BstAPI GCANNNNNTGC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 894
BstBAI YACGTR 1 cut(s) 440
BstC8I GCNNGC 1 cut(s) 1241
BstDEI CTNAG 4 cut(s) 338, 503, 1616, 1737
BstF5I GGATG 4 cut(s) 53, 88, 1492, 1614
BstFNI CGCG 2 cut(s) 630, 1288
BstKTI GATC 7 cut(s) 177, 532, 691, 1093, 1318, 1524, 1701
BstMAI GTCTC 4 cut(s) 336, 793, 1164, 1349
BstMBI GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
BstMWI GCNNNNNNNGC 2 cut(s) 142, 691
BstNI CCWGG 4 cut(s) 744, 984, 1184, 1497
BstNSI RCATGY 3 cut(s) 564, 1663, 1717
BstPAI GACNNNNGTC 1 cut(s) 780
BstSCI CCNGG 5 cut(s) 177, 742, 982, 1182, 1495
BstSFI CTRYAG 4 cut(s) 311, 964, 1177, 1221
BstSLI GKGCMC 1 cut(s) 1245
BstUI CGCG 2 cut(s) 630, 1288
BstV1I GCAGC 6 cut(s) 98, 1121, 1191, 1293, 1353, 1704
BstV2I GAAGAC 2 cut(s) 323, 809
BstX2I RGATCY 5 cut(s) 174, 529, 1090, 1521, 1698
BstYI RGATCY 5 cut(s) 174, 529, 1090, 1521, 1698
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 2 cut(s) 663, 1340
BtsCI GGATG 4 cut(s) 53, 88, 1492, 1614
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 5 cut(s) 876, 1174, 1201, 1613, 1646
Cac8I GCNNGC 1 cut(s) 1241
CaiI CAGNNNCTG 1 cut(s) 878
CseI GACGC 3 cut(s) 353, 933, 1294
Csp6I GTAC 8 cut(s) 72, 632, 719, 895, 1159, 1192, 1377, 1419
CviQI GTAC 8 cut(s) 72, 632, 719, 895, 1159, 1192, 1377, 1419
DdeI CTNAG 4 cut(s) 338, 503, 1616, 1737
DpnI GATC 7 cut(s) 176, 531, 690, 1092, 1317, 1523, 1700
DpnII GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
DraI TTTAAA 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 1341
Eco24I GRGCYC 1 cut(s) 147
Eco31I GGTCTC 1 cut(s) 1164
Eco32I GATATC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 1665
Eco88I CYCGRG 3 cut(s) 1040, 1142, 1392
EcoRI GAATTC 1 cut(s) 1705
EcoRII CCWGG 4 cut(s) 742, 982, 1182, 1495
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 1341
EcoT22I ATGCAT 2 cut(s) 687, 1442
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 1341
Esp3I CGTCTC 1 cut(s) 336
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 2 cut(s) 1246, 1594
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 6 cut(s) 87, 1135, 1180, 1307, 1367, 1718
FokI GGATG 4 cut(s) 40, 95, 1499, 1621
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 6 cut(s) 87, 1135, 1180, 1307, 1367, 1718
FspBI CTAG 5 cut(s) 263, 395, 695, 885, 938
GluI GCNGC 6 cut(s) 87, 1135, 1180, 1307, 1367, 1718
GsaI CCCAGC 1 cut(s) 1418
GsuI CTGGAG 1 cut(s) 956
HaeIII GGCC 2 cut(s) 663, 1340
HapII CCGG 3 cut(s) 178, 734, 1525
HgaI GACGC 3 cut(s) 353, 933, 1294
Hin1I GRCGYC 1 cut(s) 925
HpaII CCGG 3 cut(s) 178, 734, 1525
HphI GGTGA 2 cut(s) 587, 1262
Hpy166II GTNNAC 4 cut(s) 72, 968, 1228, 1243
Hpy188I TCNGA 7 cut(s) 275, 292, 308, 1217, 1601, 1634, 1711
Hpy8I GTNNAC 4 cut(s) 72, 968, 1228, 1243
Hpy99I CGWCG 1 cut(s) 1288
HpyAV CCTTC 5 cut(s) 242, 290, 1055, 1276, 1330
HpyCH4III ACNGT 3 cut(s) 406, 1196, 1532
HpyCH4IV ACGT 4 cut(s) 366, 439, 751, 777
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 691
HpyF3I CTNAG 4 cut(s) 338, 503, 1616, 1737
HpySE526I ACGT 4 cut(s) 366, 439, 751, 777
Hsp92I GRCGYC 1 cut(s) 925
Kpn2I TCCGGA 1 cut(s) 1524
Kzo9I GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
LmnI GCTCC 4 cut(s) 133, 1466, 1541, 1692
Lsp1109I GCAGC 6 cut(s) 98, 1121, 1191, 1293, 1353, 1704
LweI GCATC 2 cut(s) 73, 508
MaeI CTAG 5 cut(s) 263, 395, 695, 885, 938
MaeII ACGT 4 cut(s) 366, 439, 751, 777
MaeIII GTNAC 7 cut(s) 358, 400, 512, 652, 940, 1611, 1644
MalI GATC 7 cut(s) 176, 531, 690, 1092, 1317, 1523, 1700
MboI GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
MboII GAAGA 5 cut(s) 56, 161, 328, 814, 968
MfeI CAATTG 1 cut(s) 1204
MflI RGATCY 5 cut(s) 174, 529, 1090, 1521, 1698
MhlI GDGCHC 3 cut(s) 147, 1245, 1471
MlsI TGGCCA 1 cut(s) 663
MluNI TGGCCA 1 cut(s) 663
MlyI GAGTC 4 cut(s) 313, 580, 958, 963
MmeI TCCRAC 3 cut(s) 82, 331, 901
Mox20I TGGCCA 1 cut(s) 663
Mph1103I ATGCAT 2 cut(s) 687, 1442
MroI TCCGGA 1 cut(s) 1524
MroXI GAANNNNTTC 1 cut(s) 109
MscI TGGCCA 1 cut(s) 663
MseI TTAA 6 cut(s) 65, 570, 1017, 1035, 1079, 1595
MslI CAYNNNNRTG 3 cut(s) 67, 1233, 1248
Msp20I TGGCCA 1 cut(s) 663
MspA1I CMGCKG 1 cut(s) 1424
MspCI CTTAAG 1 cut(s) 1078
MspI CCGG 3 cut(s) 178, 734, 1525
MspR9I CCNGG 5 cut(s) 179, 744, 984, 1184, 1497
MunI CAATTG 1 cut(s) 1204
Mva1269I GAATGC 3 cut(s) 624, 865, 965
MvaI CCWGG 4 cut(s) 744, 984, 1184, 1497
MvnI CGCG 2 cut(s) 630, 1288
MwoI GCNNNNNNNGC 2 cut(s) 142, 691
NciI CCSGG 1 cut(s) 179
NdeII GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
NlaIV GGNNCC 5 cut(s) 176, 531, 1523, 1537, 1694
NmuCI GTSAC 4 cut(s) 358, 400, 1611, 1644
NsiI ATGCAT 2 cut(s) 687, 1442
NspI RCATGY 3 cut(s) 564, 1663, 1717
OliI CACNNNNGTG 1 cut(s) 1248
PaeR7I CTCGAG 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 1285
PctI GAATGC 3 cut(s) 624, 865, 965
PdmI GAANNNNTTC 1 cut(s) 109
PfeI GAWTC 8 cut(s) 110, 676, 728, 1010, 1073, 1492, 1564, 1635
PfoI TCCNGGA 1 cut(s) 982
PkrI GCNGC 6 cut(s) 88, 1136, 1181, 1308, 1368, 1719
PleI GAGTC 4 cut(s) 313, 580, 957, 963
PpsI GAGTC 4 cut(s) 313, 580, 957, 963
Ppu21I YACGTR 1 cut(s) 440
PshAI GACNNNNGTC 1 cut(s) 780
PshBI ATTAAT 2 cut(s) 1017, 1595
PsiI TTATAA 2 cut(s) 225, 468
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 4 cut(s) 742, 982, 1182, 1495
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 4 cut(s) 742, 982, 1182, 1495
PspN4I GGNNCC 5 cut(s) 176, 531, 1523, 1537, 1694
PspXI VCTCGAGB 1 cut(s) 1040
PstI CTGCAG 1 cut(s) 1181
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 5 cut(s) 174, 529, 1090, 1521, 1698
PvuII CAGCTG 1 cut(s) 1424
RsaI GTAC 8 cut(s) 73, 633, 720, 896, 1160, 1193, 1378, 1420
RsaNI GTAC 8 cut(s) 72, 632, 719, 895, 1159, 1192, 1377, 1419
RseI CAYNNNNRTG 3 cut(s) 67, 1233, 1248
SaqAI TTAA 6 cut(s) 65, 570, 1017, 1035, 1079, 1595
SatI GCNGC 6 cut(s) 87, 1135, 1180, 1307, 1367, 1718
Sau3AI GATC 7 cut(s) 174, 529, 688, 1090, 1315, 1521, 1698
SchI GAGTC 4 cut(s) 313, 580, 958, 963
ScrFI CCNGG 5 cut(s) 179, 744, 984, 1184, 1497
SduI GDGCHC 3 cut(s) 147, 1245, 1471
SfaNI GCATC 2 cut(s) 73, 508
SfcI CTRYAG 4 cut(s) 311, 964, 1177, 1221
Sfr274I CTCGAG 1 cut(s) 1040
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 3 cut(s) 67, 1233, 1248
SmlI CTYRAG 7 cut(s) 381, 771, 929, 946, 997, 1040, 1078
SmoI CTYRAG 7 cut(s) 381, 771, 929, 946, 997, 1040, 1078
SpeI ACTAGT 1 cut(s) 937
SsiI CCGC 1 cut(s) 1464
SspI AATATT 1 cut(s) 603
SspMI CTAG 5 cut(s) 263, 395, 695, 885, 938
StyD4I CCNGG 5 cut(s) 177, 742, 982, 1182, 1495
StyI CCWWGG 1 cut(s) 1341
TaaI ACNGT 3 cut(s) 406, 1196, 1532
TaiI ACGT 4 cut(s) 369, 442, 754, 780
TaqI TCGA 3 cut(s) 147, 1041, 1279
TatI WGTACW 2 cut(s) 894, 1158
TfiI GAWTC 8 cut(s) 110, 676, 728, 1010, 1073, 1492, 1564, 1635
Tru1I TTAA 6 cut(s) 65, 570, 1017, 1035, 1079, 1595
Tru9I TTAA 6 cut(s) 65, 570, 1017, 1035, 1079, 1595
TscAI CASTG 5 cut(s) 883, 1181, 1201, 1620, 1653
TseFI GTSAC 4 cut(s) 358, 400, 1611, 1644
TseI GCWGC 6 cut(s) 86, 1134, 1179, 1306, 1366, 1717
Tsp45I GTSAC 4 cut(s) 358, 400, 1611, 1644
TspDTI ATGAA 3 cut(s) 30, 309, 899
TspGWI ACGGA 2 cut(s) 1534, 1603
TspRI CASTG 5 cut(s) 883, 1181, 1201, 1620, 1653
Vha464I CTTAAG 1 cut(s) 1078
VneI GTGCAC 1 cut(s) 1241
VspI ATTAAT 2 cut(s) 1017, 1595
XapI RAATTY 7 cut(s) 280, 489, 498, 796, 827, 866, 1705
XceI RCATGY 3 cut(s) 564, 1663, 1717
XhoI CTCGAG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 5 cut(s) 263, 395, 695, 885, 938
Zsp2I ATGCAT 2 cut(s) 687, 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.