Rroxscaffold_3G00267550

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019807
Physical Location & Seq
Forward (+)
60620589 .. 60627130
6542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267550.1

Sequence Viewer

Length: 654 bp
ATGCTTGACAGGTATGAATATCTATTTATACAAAAGATTGTTCAAGAGATATCATCCCAAACATTAAAGTGTACCTATTTGGATGTAGCCAAATATCCCGTTGGAATAGATTCTTGTTTAGACGAGATAGAGGAGCTTTTAGGGCTCGAAGAAAATGATATTCGCATGGTTGGGATATGGGGCAGTGGTGGTATAGGTAAAACCACACTTGCTAAAGCTGTTTATAATTCTTTTGCTCACAGCTTTGAAGTTGATTCAATATATGAGGTCAAAAAATTAGATGCTCATGAAGCCCTCAAGCTTTTCAGTTGGCATGCCTTCGAAAGAAATGAACCTTTACCCAATTATGCGAAACTCACACAATGTGCGGTAGAATATGCTCAAGGGATTCCCCTAGCTTTGACAGTTTTAGGTTCTAATCTTTTTGGTAAAAGCATAGGCGAATGGCAAGATACATTAGATTGTTATAAAAGAGTTCCCAACCAACAAATTCAAGAAATTCTTAGAATTAGTTACGATGCTTTAAAGGATCCCGTGAAAGAATTATTGCTTGATATTGCATGTTTCTTTAAAGGTGAAAGCATGACTCATGTTAGACAAATATTAGAAGGTTGTGTGAGTGGCATTCAAGTACGAAAGAAAAGTCCCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

217

Amino Acids

24.55

Weight (kDa)

5.19

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 41 - 83 1.2e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019807)

Species Orthologous Gene IDs
prunus_persica Prupe.1G018600_v2.0.a1
rosa_laevigata RLG00000004790
rosa_multiflora Rmu_sc0001758.1_g000009 Rmu_sc0029164.1_g000001 Rmu_sc0029165.1_g000001
rosa_roxburghii Rroxscaffold_3G00267550
rosa_samantha Rh7CG086500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 225, 468
AciI CCGC 1 cut(s) 368
AclWI GGATC 2 cut(s) 524, 537
AcsI RAATTY 2 cut(s) 489, 498
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 2 cut(s) 73, 633
AgsI TTSAA 5 cut(s) 44, 248, 258, 494, 629
AluBI AGCT 5 cut(s) 136, 218, 243, 301, 398
AluI AGCT 5 cut(s) 136, 218, 243, 301, 398
AlwI GGATC 2 cut(s) 524, 537
ApoI RAATTY 2 cut(s) 489, 498
ArsI GACNNNNNNTTYG 1 cut(s) 628
Asp700I GAANNNNTTC 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 587
AsuII TTCGAA 1 cut(s) 321
BamHI GGATCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 1 cut(s) 649
BfaI CTAG 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 531
BmsI GCATC 2 cut(s) 271, 508
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 2 cut(s) 281, 366
BseGI GGATG 2 cut(s) 53, 88
BseRI GAGGAG 1 cut(s) 146
BslFI GGGAC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 624
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 1 cut(s) 529
BspACI CCGC 1 cut(s) 368
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 531
BspPI GGATC 2 cut(s) 524, 537
BspT104I TTCGAA 1 cut(s) 321
BssMI GATC 1 cut(s) 529
BssSI CACGAG 1 cut(s) 649
Bst2BI CACGAG 1 cut(s) 649
Bst4CI ACNGT 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 503
BstF5I GGATG 2 cut(s) 53, 88
BstKTI GATC 1 cut(s) 532
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 2 cut(s) 142, 290
BstNSI RCATGY 2 cut(s) 317, 564
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BtsCI GGATG 2 cut(s) 53, 88
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 315
CciI TCATGA 1 cut(s) 286
Csp6I GTAC 2 cut(s) 72, 632
CviAII CATG 6 cut(s) 166, 287, 314, 561, 583, 590
CviJI RGCY 8 cut(s) 89, 136, 145, 218, 243, 293, 301, 398
CviKI_1 RGCY 8 cut(s) 89, 136, 145, 218, 243, 293, 301, 398
CviQI GTAC 2 cut(s) 72, 632
DdeI CTNAG 1 cut(s) 503
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
DraI TTTAAA 2 cut(s) 525, 571
DraIII CACNNNGTG 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 51
EcoRV GATATC 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 6 cut(s) 169, 290, 317, 564, 586, 593
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 1 cut(s) 630
FatI CATG 6 cut(s) 165, 286, 313, 560, 582, 589
FokI GGATG 2 cut(s) 40, 95
FriOI GRGCYC 1 cut(s) 147
FspBI CTAG 1 cut(s) 395
Hin1II CATG 6 cut(s) 169, 290, 317, 564, 586, 593
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 4 cut(s) 110, 254, 388, 586
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 1 cut(s) 72
Hpy188III TCNNGA 4 cut(s) 44, 287, 494, 651
Hpy8I GTNNAC 1 cut(s) 72
HpyAV CCTTC 2 cut(s) 328, 602
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 290
HpyF3I CTNAG 1 cut(s) 503
Hsp92II CATG 6 cut(s) 169, 290, 317, 564, 586, 593
Kzo9I GATC 1 cut(s) 529
LmnI GCTCC 1 cut(s) 133
LweI GCATC 2 cut(s) 271, 508
MaeI CTAG 1 cut(s) 395
MaeIII GTNAC 1 cut(s) 512
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 1 cut(s) 161
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 7 cut(s) 226, 275, 343, 489, 498, 507, 542
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 3 cut(s) 124, 259, 305
MroXI GAANNNNTTC 1 cut(s) 109
MseI TTAA 3 cut(s) 65, 524, 570
MslI CAYNNNNRTG 1 cut(s) 67
Mva1269I GAATGC 1 cut(s) 624
MwoI GCNNNNNNNGC 2 cut(s) 142, 290
NdeII GATC 1 cut(s) 529
NlaIII CATG 6 cut(s) 169, 290, 317, 564, 586, 593
NlaIV GGNNCC 1 cut(s) 531
NspI RCATGY 2 cut(s) 317, 564
NspV TTCGAA 1 cut(s) 321
PaeI GCATGC 1 cut(s) 317
PagI TCATGA 1 cut(s) 286
PctI GAATGC 1 cut(s) 624
PdmI GAANNNNTTC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 110, 254, 388
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PsiI TTATAA 2 cut(s) 225, 468
PspN4I GGNNCC 1 cut(s) 531
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 2 cut(s) 73, 633
RsaNI GTAC 2 cut(s) 72, 632
RseI CAYNNNNRTG 1 cut(s) 67
SaqAI TTAA 3 cut(s) 65, 524, 570
Sau3AI GATC 1 cut(s) 529
SchI GAGTC 1 cut(s) 580
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 2 cut(s) 271, 508
SfuI TTCGAA 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 2 cut(s) 296, 381
SmoI CTYRAG 2 cut(s) 296, 381
SphI GCATGC 1 cut(s) 317
Sse9I AATT 7 cut(s) 226, 275, 343, 489, 498, 507, 542
SsiI CCGC 1 cut(s) 368
SspI AATATT 1 cut(s) 603
SspMI CTAG 1 cut(s) 395
TaaI ACNGT 1 cut(s) 406
TaqI TCGA 2 cut(s) 147, 321
TasI AATT 7 cut(s) 226, 275, 343, 489, 498, 507, 542
TfiI GAWTC 3 cut(s) 110, 254, 388
Tru1I TTAA 3 cut(s) 65, 524, 570
Tru9I TTAA 3 cut(s) 65, 524, 570
TscAI CASTG 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 30, 303, 345
TspRI CASTG 1 cut(s) 190
XapI RAATTY 2 cut(s) 489, 498
XceI RCATGY 2 cut(s) 317, 564
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.