RLG00000005414

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017667
Physical Location & Seq
Forward (+)
67483792 .. 67484706
915 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005414

Sequence Viewer

Length: 768 bp
ATGGCTAGTCTCCGGTCCTGTTTCGTCATAGTCATGATTGTTCTTAACCTAGGCATGGCTGCCGCCGGTCTCTCTCTTAAGCGTGGTTGGCAGCATGCTAGTGCAACGTTTTACGGCGACACAAATGGTGCTGAAACCTTGCAGGGAGCCTGTGGATATGAAAATGTTATTGAAGAAGGATACGGGCTTCAAACCACAGCATTAAGCGAGGCATTGTTCAACAAAGGACGTACTTGCGGAGCTTGTTTCCAGATTATGTGTTTTAACTCTTCAGAATGGTGCAAAAAAGGTGCAGGCGCAATCCGAGTCACAGCTACCAACTACTGCCCTCCAAGCTACAATGAGGAGGCTCCATGGTGCAACCCACCTCGCAAGCACTTTGATCTATCCATGCCTATGTTCTTGAACATTGCAGAGTACAAAGCAGGAATTGTCCCGGTTGTCTATCGCCGAGTTAAGTGCCGCAAGAGTGGTGGTATTAAGTTCGTGTTTAAGGGAAATCAAGGTTGGTTGCTTGTGCTAGTGTACAATGTTGGTGGTGCTGGACAGGTTGTTGATGTGAAGATAAAGGGTTCACAGACCGGTTGGATACAAATGGAACGTAATTGGGGCGTAAACTGGCAGGTATCGGATGATACCGTGCAGAAGTTACAGGGGCAGAGCTTGTCTTTCCAAGTTACCACAAGGGATGGTAGAATGGTGCAGTCCGACAATGTCGCCCCGACGGATTGGCAATTGGGGAAGACATACGAGGGAAGAAACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

256

Amino Acids

28.17

Weight (kDa)

8.94

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 33 - 132 6.4e-07 EXPB1-like domain 1
DPBB_1 PF03330 65 - 149 7.4e-20 Lytic transglycolase
Expansin_C PF01357 160 - 241 1.1e-23 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17040
rosa_chinensis RchiOBHm_Chr7g0178811
rosa_laevigata RLG00000005414
rosa_multiflora Rmu_sc0015029.1_g000005 Rmu_sc0025325.1_g000006
rosa_roxburghii Rroxscaffold_3G00274590
rosa_rugosa Rorug06G0419900
rosa_samantha Rh7AG019200 Rh7BG018500 Rh7CG019300 Rh7DG019100
rosa_wichuraiana Rw7G001560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AciI CCGC 3 cut(s) 63, 237, 463
AclI AACGTT 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 3 cut(s) 232, 419, 527
AfiI CCNNNNNNNGG 1 cut(s) 55
AflII CTTAAG 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 581
AgsI TTSAA 4 cut(s) 173, 191, 220, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 4 cut(s) 242, 314, 336, 663
AluI AGCT 4 cut(s) 242, 314, 336, 663
Alw26I GTCTC 2 cut(s) 14, 74
ApeKI GCWGC 2 cut(s) 59, 91
AsiGI ACCGGT 1 cut(s) 581
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 15
AsuC2I CCSGG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 15
AvrII CCTAGG 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 749
BbvI GCAGC 2 cut(s) 46, 103
BccI CCATC 1 cut(s) 683
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BciVI GTATCC 2 cut(s) 173, 582
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 14, 74
BfaI CTAG 5 cut(s) 6, 50, 99, 521, 766
BfrI CTTAAG 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 2 cut(s) 173, 582
BisI GCNGC 4 cut(s) 60, 63, 92, 463
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 4 cut(s) 61, 64, 93, 464
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 148, 351
BmrFI CCNGG 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 749
BpuMI CCSGG 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 49, 353
BsaWI WCCGGW 2 cut(s) 12, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 2 cut(s) 65, 581
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 408
BseDI CCNNGG 2 cut(s) 49, 353
BseGI GGATG 2 cut(s) 637, 694
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 623
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 2 cut(s) 46, 103
BsgI GTGCAG 3 cut(s) 312, 662, 722
BshTI ACCGGT 1 cut(s) 581
BsiSI CCGG 4 cut(s) 13, 66, 437, 582
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 2 cut(s) 14, 74
BsmFI GGGAC 1 cut(s) 419
Bso31I GGTCTC 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 525
Bsp143I GATC 1 cut(s) 382
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 3 cut(s) 63, 237, 463
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 148, 351
BspMI ACCTGC 1 cut(s) 613
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 2 cut(s) 65, 581
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 623
BssAI RCCGGY 2 cut(s) 65, 581
BssECI CCNNGG 2 cut(s) 49, 353
BssMI GATC 1 cut(s) 382
BssT1I CCWWGG 2 cut(s) 49, 353
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 274
BstAFI CTTAAG 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 525
BstC8I GCNNGC 3 cut(s) 96, 295, 374
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 637, 694
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 2 cut(s) 14, 74
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 2 cut(s) 88, 333
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 46, 103
BstV2I GAAGAC 1 cut(s) 749
BsuI GTATCC 2 cut(s) 173, 582
BtgI CCRYGG 1 cut(s) 353
BtsCI GGATG 2 cut(s) 637, 694
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 3 cut(s) 96, 295, 374
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 2 cut(s) 65, 581
Cfr13I GGNCC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 231, 418, 526
CspAI ACCGGT 1 cut(s) 581
CspCI CAANNNNNGTGG 4 cut(s) 454, 489, 517, 552
CviAII CATG 5 cut(s) 34, 55, 95, 354, 391
CviJI RGCY 9 cut(s) 5, 59, 149, 187, 242, 314, 336, 350, 663
CviKI_1 RGCY 9 cut(s) 5, 59, 149, 187, 242, 314, 336, 350, 663
CviQI GTAC 3 cut(s) 231, 418, 526
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 274
Eco130I CCWWGG 2 cut(s) 49, 353
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 49, 353
ErhI CCWWGG 2 cut(s) 49, 353
FaeI CATG 5 cut(s) 37, 58, 98, 357, 394
FaqI GGGAC 1 cut(s) 419
FatI CATG 5 cut(s) 33, 54, 94, 353, 390
Fnu4HI GCNGC 4 cut(s) 60, 63, 92, 463
FokI GGATG 2 cut(s) 644, 701
Fsp4HI GCNGC 4 cut(s) 60, 63, 92, 463
FspBI CTAG 5 cut(s) 6, 50, 99, 521, 766
GlaI GCGC 1 cut(s) 298
GluI GCNGC 4 cut(s) 60, 63, 92, 463
HapII CCGG 4 cut(s) 13, 66, 437, 582
HhaI GCGC 1 cut(s) 299
Hin1II CATG 5 cut(s) 37, 58, 98, 357, 394
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 1 cut(s) 306
HpaII CCGG 4 cut(s) 13, 66, 437, 582
Hpy166II GTNNAC 3 cut(s) 526, 575, 616
Hpy188I TCNGA 4 cut(s) 274, 305, 631, 709
Hpy188III TCNNGA 3 cut(s) 34, 250, 403
Hpy8I GTNNAC 3 cut(s) 526, 575, 616
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 3 cut(s) 107, 229, 601
HpyCH4V TGCA 8 cut(s) 104, 142, 282, 293, 360, 413, 643, 703
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 333
HpySE526I ACGT 3 cut(s) 107, 229, 601
Hsp92II CATG 5 cut(s) 37, 58, 98, 357, 394
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 3 cut(s) 146, 239, 355
Lsp1109I GCAGC 2 cut(s) 46, 103
MaeI CTAG 5 cut(s) 6, 50, 99, 521, 766
MaeII ACGT 3 cut(s) 107, 229, 601
MaeIII GTNAC 3 cut(s) 307, 648, 676
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 5 cut(s) 185, 261, 574, 754, 768
MfeI CAATTG 1 cut(s) 734
MluCI AATT 3 cut(s) 429, 604, 734
MlyI GAGTC 1 cut(s) 315
MmeI TCCRAC 2 cut(s) 566, 732
MnlI CCTC 6 cut(s) 202, 337, 339, 340, 378, 745
MseI TTAA 7 cut(s) 45, 78, 203, 264, 456, 480, 492
MslI CAYNNNNRTG 3 cut(s) 32, 99, 395
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 4 cut(s) 13, 66, 437, 582
MspR9I CCNGG 1 cut(s) 437
MunI CAATTG 1 cut(s) 734
MwoI GCNNNNNNNGC 2 cut(s) 88, 333
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 353
NdeII GATC 1 cut(s) 382
NlaIII CATG 5 cut(s) 37, 58, 98, 357, 394
NlaIV GGNNCC 2 cut(s) 148, 351
NmeAIII GCCGAG 1 cut(s) 476
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 1 cut(s) 98
PaeI GCATGC 1 cut(s) 98
PagI TCATGA 1 cut(s) 33
PflFI GACNNNGTC 1 cut(s) 713
PinAI ACCGGT 1 cut(s) 581
PkrI GCNGC 4 cut(s) 61, 64, 93, 464
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Psp1406I AACGTT 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 148, 351
PspPI GGNCC 1 cut(s) 15
PsyI GACNNNGTC 1 cut(s) 713
RsaI GTAC 3 cut(s) 232, 419, 527
RsaNI GTAC 3 cut(s) 231, 418, 526
RseI CAYNNNNRTG 3 cut(s) 32, 99, 395
SaqAI TTAA 7 cut(s) 45, 78, 203, 264, 456, 480, 492
SatI GCNGC 4 cut(s) 60, 63, 92, 463
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 1 cut(s) 15
SchI GAGTC 1 cut(s) 315
ScrFI CCNGG 1 cut(s) 437
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 32, 99, 395
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 98
Sse9I AATT 3 cut(s) 429, 604, 734
SsiI CCGC 3 cut(s) 63, 237, 463
SspMI CTAG 5 cut(s) 6, 50, 99, 521, 766
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 2 cut(s) 49, 353
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 3 cut(s) 110, 232, 604
TasI AATT 3 cut(s) 429, 604, 734
TatI WGTACW 2 cut(s) 417, 525
TauI GCSGC 2 cut(s) 65, 465
Tru1I TTAA 7 cut(s) 45, 78, 203, 264, 456, 480, 492
Tru9I TTAA 7 cut(s) 45, 78, 203, 264, 456, 480, 492
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 59, 91
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 174
TspGWI ACGGA 1 cut(s) 740
Tth111I GACNNNGTC 1 cut(s) 713
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 15
XceI RCATGY 1 cut(s) 98
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 5 cut(s) 6, 50, 99, 521, 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.