Rroxscaffold_3G00274590

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017667
Physical Location & Seq
Forward (+)
66220931 .. 66221838
908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274590.1

Sequence Viewer

Length: 714 bp
ATGGCTAGTCTCCGGTTCTGTTTCGTCACAGTCATGGTTGTTCTCAACCTAGGCATGGCTGCCGCCGATCTGAAGCGTGGTTGGCAGCATGCAACTGCAACGTTTTACGGCGATATGAATGGTGATGAAACCTTGCAGGGAGCTTGTGGATATGAAAATGTTATTGAAGAAGGATACGGGCTTCAAACCACAGCATTAAGCGAGGCATTGTTCAACAAAGGACGTACTTGCGGAGCTTGTTTCCAGATAATGTGTTTTAACTCTTCAGAATGGTGCAAAAAAGGTGCAGGCGCAATCCGAGTCACAGCTACCAACTACTGCCCTCCAAGCTACGATGAGGAGGCTCCATGGTGCAACCCACCTCGCAAGCACTTTGATCTATCCATGCCTATGTTCTTGAACATTGCGGAATACAAAGCAGGAATTGTCCCGGTTGTCTATCGCCGAGTTAAGTGCCGCAAGAGTGGTGTGTACAATGTTGGTGGTGCTGGACTGGTTGTTGATGTGAAGATAAAGGGTTCACAGACTGGTTGGATACAAATGGAACGTAATTGGGGCGTAAACTGGCAGGTATCGGATGATACCGTGCAGAAGTTACAGGGGCAGAGCTTGTCTTTCCAAGTTACCGCAAGGGATGGTAGAATGGTGCAGTCCGACAATGTCGCCCCGAAGGATTGGCAATTGGGGAAGACATATGAGGGAAGAAACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

237

Amino Acids

26.33

Weight (kDa)

8.33

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 31 - 128 1.7e-06 EXPB1-like domain 1
DPBB_1 PF03330 63 - 147 6.3e-20 Lytic transglycolase
Expansin_C PF01357 157 - 223 5.1e-18 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17040
rosa_chinensis RchiOBHm_Chr7g0178811
rosa_laevigata RLG00000005414
rosa_multiflora Rmu_sc0015029.1_g000005 Rmu_sc0025325.1_g000006
rosa_roxburghii Rroxscaffold_3G00274590
rosa_rugosa Rorug06G0419900
rosa_samantha Rh7AG019200 Rh7BG018500 Rh7CG019300 Rh7DG019100
rosa_wichuraiana Rw7G001560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AciI CCGC 5 cut(s) 63, 231, 407, 457, 627
AclI AACGTT 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 92, 249
AfaI GTAC 2 cut(s) 226, 473
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 4 cut(s) 167, 185, 214, 400
AluBI AGCT 5 cut(s) 143, 236, 308, 330, 609
AluI AGCT 5 cut(s) 143, 236, 308, 330, 609
Alw26I GTCTC 1 cut(s) 14
ApeKI GCWGC 2 cut(s) 59, 85
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 134
AvrII CCTAGG 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 695
BbvI GCAGC 2 cut(s) 46, 97
BccI CCATC 1 cut(s) 629
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciVI GTATCC 2 cut(s) 167, 528
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 3 cut(s) 6, 50, 712
BfuAI ACCTGC 1 cut(s) 559
BfuI GTATCC 2 cut(s) 167, 528
BisI GCNGC 4 cut(s) 60, 63, 86, 457
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 4 cut(s) 61, 64, 87, 458
Bme1390I CCNGG 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 695
BpuMI CCSGG 1 cut(s) 431
BsaJI CCNNGG 2 cut(s) 49, 347
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 498, 532, 569
Bse3DI GCAATG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 49, 347
BseGI GGATG 2 cut(s) 583, 640
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 3 cut(s) 498, 532, 569
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 46, 97
BsgI GTGCAG 3 cut(s) 306, 608, 668
BsiSI CCGG 2 cut(s) 13, 431
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 2 cut(s) 67, 376
Bsp19I CCATGG 1 cut(s) 347
BspACI CCGC 5 cut(s) 63, 231, 407, 457, 627
BspLI GGNNCC 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 559
BsrDI GCAATG 1 cut(s) 402
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 3 cut(s) 498, 532, 569
BssECI CCNNGG 2 cut(s) 49, 347
BssMI GATC 2 cut(s) 67, 376
BssT1I CCWWGG 2 cut(s) 49, 347
Bst4CI ACNGT 2 cut(s) 31, 586
Bst6I CTCTTC 1 cut(s) 268
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 90, 289, 368
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 583, 640
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 70, 379
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 2 cut(s) 67, 376
BstMWI GCNNNNNNNGC 2 cut(s) 82, 327
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 46, 97
BstV2I GAAGAC 1 cut(s) 695
BsuI GTATCC 2 cut(s) 167, 528
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 2 cut(s) 583, 640
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 3 cut(s) 90, 289, 368
CfoI GCGC 1 cut(s) 293
Csp6I GTAC 2 cut(s) 225, 472
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 5 cut(s) 34, 55, 89, 348, 385
CviJI RGCY 9 cut(s) 5, 59, 143, 181, 236, 308, 330, 344, 609
CviKI_1 RGCY 9 cut(s) 5, 59, 143, 181, 236, 308, 330, 344, 609
CviQI GTAC 2 cut(s) 225, 472
DpnI GATC 2 cut(s) 69, 378
DpnII GATC 2 cut(s) 67, 376
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 49, 347
Eco57I CTGAAG 2 cut(s) 92, 249
EcoT14I CCWWGG 2 cut(s) 49, 347
ErhI CCWWGG 2 cut(s) 49, 347
FaeI CATG 5 cut(s) 37, 58, 92, 351, 388
FaqI GGGAC 1 cut(s) 413
FatI CATG 5 cut(s) 33, 54, 88, 347, 384
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 4 cut(s) 60, 63, 86, 457
FokI GGATG 2 cut(s) 590, 647
Fsp4HI GCNGC 4 cut(s) 60, 63, 86, 457
FspBI CTAG 3 cut(s) 6, 50, 712
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 60, 63, 86, 457
HapII CCGG 2 cut(s) 13, 431
HhaI GCGC 1 cut(s) 293
Hin1II CATG 5 cut(s) 37, 58, 92, 351, 388
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 1 cut(s) 300
HpaII CCGG 2 cut(s) 13, 431
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 3 cut(s) 472, 521, 562
Hpy188I TCNGA 5 cut(s) 72, 268, 299, 577, 655
Hpy188III TCNNGA 2 cut(s) 244, 397
Hpy8I GTNNAC 3 cut(s) 472, 521, 562
HpyAV CCTTC 2 cut(s) 164, 664
HpyCH4III ACNGT 2 cut(s) 31, 586
HpyCH4IV ACGT 3 cut(s) 101, 223, 547
HpyCH4V TGCA 8 cut(s) 92, 98, 136, 276, 287, 354, 589, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 327
HpySE526I ACGT 3 cut(s) 101, 223, 547
Hsp92II CATG 5 cut(s) 37, 58, 92, 351, 388
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 67, 376
LmnI GCTCC 3 cut(s) 140, 233, 349
Lsp1109I GCAGC 2 cut(s) 46, 97
MaeI CTAG 3 cut(s) 6, 50, 712
MaeII ACGT 3 cut(s) 101, 223, 547
MaeIII GTNAC 4 cut(s) 25, 301, 594, 622
MalI GATC 2 cut(s) 69, 378
MboI GATC 2 cut(s) 67, 376
MboII GAAGA 5 cut(s) 179, 255, 520, 700, 714
MfeI CAATTG 1 cut(s) 680
MluCI AATT 3 cut(s) 423, 550, 680
MlyI GAGTC 1 cut(s) 309
MmeI TCCRAC 2 cut(s) 512, 678
MnlI CCTC 6 cut(s) 196, 331, 333, 334, 372, 691
MseI TTAA 3 cut(s) 197, 258, 450
MslI CAYNNNNRTG 2 cut(s) 32, 389
MspI CCGG 2 cut(s) 13, 431
MspR9I CCNGG 1 cut(s) 431
MunI CAATTG 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 82, 327
NciI CCSGG 1 cut(s) 431
NcoI CCATGG 1 cut(s) 347
NdeI CATATG 1 cut(s) 694
NdeII GATC 2 cut(s) 67, 376
NlaIII CATG 5 cut(s) 37, 58, 92, 351, 388
NlaIV GGNNCC 1 cut(s) 345
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 2 cut(s) 25, 301
NspI RCATGY 1 cut(s) 92
PaeI GCATGC 1 cut(s) 92
PflFI GACNNNGTC 1 cut(s) 659
PkrI GCNGC 4 cut(s) 61, 64, 87, 458
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 345
PsyI GACNNNGTC 1 cut(s) 659
RsaI GTAC 2 cut(s) 226, 473
RsaNI GTAC 2 cut(s) 225, 472
RseI CAYNNNNRTG 2 cut(s) 32, 389
SaqAI TTAA 3 cut(s) 197, 258, 450
SatI GCNGC 4 cut(s) 60, 63, 86, 457
Sau3AI GATC 2 cut(s) 67, 376
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 431
SmiMI CAYNNNNRTG 2 cut(s) 32, 389
SphI GCATGC 1 cut(s) 92
Sse9I AATT 3 cut(s) 423, 550, 680
SsiI CCGC 5 cut(s) 63, 231, 407, 457, 627
SspMI CTAG 3 cut(s) 6, 50, 712
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 49, 347
TaaI ACNGT 2 cut(s) 31, 586
TaiI ACGT 3 cut(s) 104, 226, 550
TasI AATT 3 cut(s) 423, 550, 680
TatI WGTACW 1 cut(s) 471
TauI GCSGC 2 cut(s) 65, 459
Tru1I TTAA 3 cut(s) 197, 258, 450
Tru9I TTAA 3 cut(s) 197, 258, 450
TseFI GTSAC 2 cut(s) 25, 301
TseI GCWGC 2 cut(s) 59, 85
Tsp45I GTSAC 2 cut(s) 25, 301
TspDTI ATGAA 3 cut(s) 131, 141, 168
Tth111I GACNNNGTC 1 cut(s) 659
XceI RCATGY 1 cut(s) 92
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 3 cut(s) 6, 50, 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.