Rmu_sc0025325.1_g000006

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0025325.1
OG0017667
Physical Location & Seq
Forward (+)
24835 .. 25749
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0025325.1_g000006.1.cds

Sequence Viewer

Length: 711 bp
atgactagtctccggtcctgtttcgtcatagtcatgattgttctcaacctaggcatggctgccgccggtctctctcttaagcgtggttggcagcatgctactgcaacgttttacggcgacatgaatggtgatgaaaccttgccattaagcgaggcattgttcaacaaaggacgtacttgcggagcttgtttccaaataatgtgttttaactcttcagaatggtgcaaaaaaggtgcaggtgcaatccgggtcacagctaccaactactgccctccaagctacgatgaggaagctccttggtgcaacccgcctcgcaggcactttgatctatccatgcctatgttcttgaacattgcagaatacaaagcaggaattgtcccggttgtctatcgccgagttaagtgccgcaagagtggtggtatcaagttcgtgtttaagggaaatcaaggttggttgcttgtgctagtgtacaatgttggtggtgctggacaggttgttgatgtgaagataaagggttcacagactggttggatacaaatggaacgtaattggggcgtaaactggcaggtatcagatgataccgtgcagaagttacaggggcagagcttgtctttccaagttaccgcaaggaatggtagaatggtgcagtccgacaatgtcgccccgagggattggcaattggggaagacatatgagggaagaaacttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

236

Amino Acids

26.36

Weight (kDa)

9.33

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17040
rosa_chinensis RchiOBHm_Chr7g0178811
rosa_laevigata RLG00000005414
rosa_multiflora Rmu_sc0015029.1_g000005 Rmu_sc0025325.1_g000006
rosa_roxburghii Rroxscaffold_3G00274590
rosa_rugosa Rorug06G0419900
rosa_samantha Rh7AG019200 Rh7BG018500 Rh7CG019300 Rh7DG019100
rosa_wichuraiana Rw7G001560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 227
Acc36I ACCTGC 2 cut(s) 227, 556
AciI CCGC 5 cut(s) 63, 180, 308, 406, 624
AclI AACGTT 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 198
AfaI GTAC 2 cut(s) 175, 470
AfiI CCNNNNNNNGG 1 cut(s) 55
AflII CTTAAG 1 cut(s) 77
AgsI TTSAA 2 cut(s) 163, 349
AhlI ACTAGT 1 cut(s) 5
AjuI GAANNNNNNNTTGG 2 cut(s) 433, 465
AluBI AGCT 5 cut(s) 185, 257, 279, 293, 606
AluI AGCT 5 cut(s) 185, 257, 279, 293, 606
Alw26I GTCTC 2 cut(s) 14, 74
Ama87I CYCGRG 1 cut(s) 664
ApeKI GCWGC 2 cut(s) 59, 91
AspA2I CCTAGG 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 15
AsuC2I CCSGG 2 cut(s) 248, 380
AsuHPI GGTGA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 1 cut(s) 15
AvrII CCTAGG 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 46, 103
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciVI GTATCC 1 cut(s) 525
BcnI CCSGG 2 cut(s) 248, 380
BcoDI GTCTC 2 cut(s) 14, 74
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 4 cut(s) 6, 50, 464, 709
BfrI CTTAAG 1 cut(s) 77
BfuAI ACCTGC 2 cut(s) 227, 556
BfuI GTATCC 1 cut(s) 525
BglI GCCNNNNNGGC 1 cut(s) 316
BisI GCNGC 4 cut(s) 60, 63, 92, 406
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 4 cut(s) 61, 64, 93, 407
Bme1390I CCNGG 2 cut(s) 248, 380
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 15
BmrFI CCNGG 2 cut(s) 248, 380
BpiI GAAGAC 1 cut(s) 692
BpuMI CCSGG 2 cut(s) 248, 380
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 3 cut(s) 49, 296, 665
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 529, 566
Bse3DI GCAATG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 49, 296, 665
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 529, 566
BseXI GCAGC 2 cut(s) 46, 103
BsgI GTGCAG 3 cut(s) 255, 605, 665
BsiHKCI CYCGRG 1 cut(s) 664
BsiSI CCGG 4 cut(s) 13, 66, 247, 380
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 2 cut(s) 14, 74
BsmFI GGGAC 1 cut(s) 362
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 1 cut(s) 325
BspACI CCGC 5 cut(s) 63, 180, 308, 406, 624
BspHI TCATGA 1 cut(s) 33
BspMI ACCTGC 2 cut(s) 227, 556
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 2 cut(s) 529, 566
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 49, 296, 665
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 2 cut(s) 49, 296
Bst4CI ACNGT 1 cut(s) 583
Bst6I CTCTTC 1 cut(s) 217
BstAFI CTTAAG 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 468
BstC8I GCNNGC 2 cut(s) 96, 317
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 2 cut(s) 14, 74
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 3 cut(s) 88, 276, 316
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 246, 378
BstV1I GCAGC 2 cut(s) 46, 103
BstV2I GAAGAC 1 cut(s) 692
BsuI GTATCC 1 cut(s) 525
BveI ACCTGC 2 cut(s) 227, 556
Cac8I GCNNGC 2 cut(s) 96, 317
CciI TCATGA 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 15
Csp6I GTAC 2 cut(s) 174, 469
CspCI CAANNNNNGTGG 4 cut(s) 397, 432, 460, 495
CviAII CATG 5 cut(s) 34, 55, 95, 121, 334
CviJI RGCY 6 cut(s) 59, 185, 257, 279, 293, 606
CviKI_1 RGCY 6 cut(s) 59, 185, 257, 279, 293, 606
CviQI GTAC 2 cut(s) 174, 469
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 49, 296
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 664
EcoT14I CCWWGG 2 cut(s) 49, 296
ErhI CCWWGG 2 cut(s) 49, 296
FaeI CATG 5 cut(s) 37, 58, 98, 124, 337
FaiI YATR 9 cut(s) 29, 35, 56, 96, 122, 335, 341, 691, 693
FaqI GGGAC 1 cut(s) 362
FatI CATG 5 cut(s) 33, 54, 94, 120, 333
FauI CCCGC 1 cut(s) 315
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 60, 63, 92, 406
Fsp4HI GCNGC 4 cut(s) 60, 63, 92, 406
FspBI CTAG 4 cut(s) 6, 50, 464, 709
GluI GCNGC 4 cut(s) 60, 63, 92, 406
HapII CCGG 4 cut(s) 13, 66, 247, 380
Hin1II CATG 5 cut(s) 37, 58, 98, 124, 337
HpaII CCGG 4 cut(s) 13, 66, 247, 380
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 3 cut(s) 469, 518, 559
Hpy188I TCNGA 3 cut(s) 217, 574, 652
Hpy188III TCNNGA 2 cut(s) 34, 346
Hpy8I GTNNAC 3 cut(s) 469, 518, 559
HpyCH4III ACNGT 1 cut(s) 583
HpyCH4IV ACGT 3 cut(s) 107, 172, 544
HpyCH4V TGCA 8 cut(s) 104, 225, 236, 242, 303, 356, 586, 646
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 276, 316
HpySE526I ACGT 3 cut(s) 107, 172, 544
Hsp92II CATG 5 cut(s) 37, 58, 98, 124, 337
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 2 cut(s) 182, 298
Lsp1109I GCAGC 2 cut(s) 46, 103
MaeI CTAG 4 cut(s) 6, 50, 464, 709
MaeII ACGT 3 cut(s) 107, 172, 544
MaeIII GTNAC 3 cut(s) 250, 591, 619
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 4 cut(s) 204, 517, 697, 711
MfeI CAATTG 1 cut(s) 677
MluCI AATT 3 cut(s) 372, 547, 677
MmeI TCCRAC 2 cut(s) 509, 675
MnlI CCTC 6 cut(s) 145, 280, 282, 321, 660, 688
MseI TTAA 5 cut(s) 78, 146, 207, 399, 435
MslI CAYNNNNRTG 2 cut(s) 32, 338
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 4 cut(s) 13, 66, 247, 380
MspR9I CCNGG 2 cut(s) 248, 380
MunI CAATTG 1 cut(s) 677
MwoI GCNNNNNNNGC 3 cut(s) 88, 276, 316
NciI CCSGG 2 cut(s) 248, 380
NdeI CATATG 1 cut(s) 691
NdeII GATC 1 cut(s) 325
NlaIII CATG 5 cut(s) 37, 58, 98, 124, 337
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 98
PaeI GCATGC 1 cut(s) 98
PagI TCATGA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 227
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 4 cut(s) 61, 64, 93, 407
Psp1406I AACGTT 1 cut(s) 107
PspPI GGNCC 1 cut(s) 15
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 2 cut(s) 175, 470
RsaNI GTAC 2 cut(s) 174, 469
RseI CAYNNNNRTG 2 cut(s) 32, 338
SaqAI TTAA 5 cut(s) 78, 146, 207, 399, 435
SatI GCNGC 4 cut(s) 60, 63, 92, 406
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 248, 380
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 32, 338
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 5
SphI GCATGC 1 cut(s) 98
Sse9I AATT 3 cut(s) 372, 547, 677
SsiI CCGC 5 cut(s) 63, 180, 308, 406, 624
SspMI CTAG 4 cut(s) 6, 50, 464, 709
StyD4I CCNGG 2 cut(s) 246, 378
StyI CCWWGG 2 cut(s) 49, 296
TaaI ACNGT 1 cut(s) 583
TaiI ACGT 3 cut(s) 110, 175, 547
TasI AATT 3 cut(s) 372, 547, 677
TatI WGTACW 1 cut(s) 468
TauI GCSGC 2 cut(s) 65, 408
Tru1I TTAA 5 cut(s) 78, 146, 207, 399, 435
Tru9I TTAA 5 cut(s) 78, 146, 207, 399, 435
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 59, 91
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 137, 147
Tth111I GACNNNGTC 1 cut(s) 656
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 15
XceI RCATGY 1 cut(s) 98
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 4 cut(s) 6, 50, 464, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.