RLG00000006534

poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0020586
Physical Location & Seq
Reverse (-)
8214824 .. 8215760
937 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006534

Sequence Viewer

Length: 447 bp
ATGATTGTTGATGATGATGACAGAAAAGGCCACGATTTCGGGTCAGATTGCTCTGAATCGAGCTCCCCCGCCTCCATTGTTTCGGATCTCTTCGAGGTTTTCAGTCGAATGAGATTGGATATGTTAGAAGAAGATGATATGGAGTATGAGTTTACGAAGAAGAAATTTTCCATGGGTATGGAATCGGCCGGAGTAGAAGCCAAGGGAGAGCCCAGGTCTCATGGCGTTGGAGTTCAATTGTCTGCTAATAACTATTCTTTTGAGGCTGATGAGAATGGGCTGAGGCACATGTTGCTATGCAAAGTCATACTGGGAAAGAGAGAATTGGACAATTTGGTGCCTCCTCACCGGGAACGACGGAGAAAATATCGGGCTCTTTTATACTTGCAGCAAGAAGAGCCACTTGCAGAGGACGAAGAGCTTTCGGTTCCTGAGTGCAACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

149

Amino Acids

16.98

Weight (kDa)

4.77

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020586)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0248971
rosa_laevigata RLG00000002300 RLG00000006534
rosa_multiflora Rmu_sc0008653.1_g000001
rosa_roxburghii Rroxscaffold_5G00371440 Rroxscaffold_7G00195990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 337
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 93
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 1 cut(s) 212
AluBI AGCT 2 cut(s) 63, 421
AluI AGCT 2 cut(s) 63, 421
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 1 cut(s) 93
AoxI GGCC 2 cut(s) 28, 186
ApeKI GCWGC 1 cut(s) 388
ApoI RAATTY 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 65, 213, 376
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 400
BciT130I CCWGG 1 cut(s) 214
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 222
BisI GCNGC 1 cut(s) 389
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 2 cut(s) 214, 350
BmiI GGNNCC 2 cut(s) 339, 429
BmrFI CCNGG 2 cut(s) 214, 350
BmrI ACTGGG 1 cut(s) 320
BmuI ACTGGG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 171, 201, 212
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse1I ACTGG 1 cut(s) 315
BseBI CCWGG 1 cut(s) 214
BseDI CCNNGG 3 cut(s) 171, 201, 212
BseMII CTCAG 2 cut(s) 272, 423
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 400
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 30, 188
BshNI GGYRCC 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 189, 349
BsmAI GTCTC 1 cut(s) 222
BsnI GGCC 2 cut(s) 30, 188
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 3 cut(s) 65, 213, 376
Bsp143I GATC 1 cut(s) 85
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 1 cut(s) 69
BspANI GGCC 2 cut(s) 30, 188
BspCNI CTCAG 2 cut(s) 273, 424
BspLI GGNNCC 2 cut(s) 339, 429
BspPI GGATC 1 cut(s) 93
BspQI GCTCTTC 2 cut(s) 390, 411
BspT107I GGYRCC 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 222
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 171, 201, 212
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 2 cut(s) 171, 201
Bst2UI CCWGG 1 cut(s) 214
Bst6I CTCTTC 3 cut(s) 95, 390, 411
BstAPI GCANNNNNTGC 1 cut(s) 292
BstDEI CTNAG 2 cut(s) 281, 432
BstDSI CCRYGG 1 cut(s) 171
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 1 cut(s) 85
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 2 cut(s) 292, 397
BstNI CCWGG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 2 cut(s) 212, 348
BstV1I GCAGC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 178
BstYI RGATCY 1 cut(s) 85
BstZI CGGCCG 1 cut(s) 186
BsuRI GGCC 2 cut(s) 30, 188
BtgI CCRYGG 1 cut(s) 171
CviAII CATG 3 cut(s) 172, 221, 289
DdeI CTNAG 2 cut(s) 281, 432
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 95, 390, 411
EarI CTCTTC 3 cut(s) 95, 390, 411
Ecl136II GAGCTC 1 cut(s) 63
EclXI CGGCCG 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 171, 201
Eco24I GRGCYC 3 cut(s) 65, 213, 376
Eco31I GGTCTC 1 cut(s) 222
Eco52I CGGCCG 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 171, 201
EcoT38I GRGCYC 3 cut(s) 65, 213, 376
ErhI CCWWGG 2 cut(s) 171, 201
FaeI CATG 3 cut(s) 175, 224, 292
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FatI CATG 3 cut(s) 171, 220, 288
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 389
FriOI GRGCYC 3 cut(s) 65, 213, 376
Fsp4HI GCNGC 1 cut(s) 389
GluI GCNGC 1 cut(s) 389
HaeIII GGCC 2 cut(s) 30, 188
HapII CCGG 2 cut(s) 189, 349
Hin1II CATG 3 cut(s) 175, 224, 292
HinfI GANTC 2 cut(s) 56, 182
HpaII CCGG 2 cut(s) 189, 349
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 153
Hpy188I TCNGA 3 cut(s) 46, 55, 85
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 153
Hpy99I CGWCG 1 cut(s) 360
HpyCH4V TGCA 4 cut(s) 300, 388, 407, 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 397
HpyF3I CTNAG 2 cut(s) 281, 432
Hsp92II CATG 3 cut(s) 175, 224, 292
Kzo9I GATC 1 cut(s) 85
LguI GCTCTTC 2 cut(s) 390, 411
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 5 cut(s) 199, 202, 226, 296, 362
Lsp1109I GCAGC 1 cut(s) 400
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 7 cut(s) 82, 140, 143, 169, 172, 407, 428
MfeI CAATTG 1 cut(s) 236
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 3 cut(s) 65, 213, 376
MluCI AATT 4 cut(s) 164, 236, 323, 331
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 7 cut(s) 82, 88, 256, 276, 351, 354, 403
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 2 cut(s) 189, 349
MspR9I CCNGG 2 cut(s) 214, 350
MunI CAATTG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 214
MwoI GCNNNNNNNGC 2 cut(s) 292, 397
NciI CCSGG 1 cut(s) 350
NcoI CCATGG 1 cut(s) 171
NdeII GATC 1 cut(s) 85
NlaIII CATG 3 cut(s) 175, 224, 292
NlaIV GGNNCC 2 cut(s) 339, 429
NspI RCATGY 1 cut(s) 292
PciI ACATGT 1 cut(s) 288
PciSI GCTCTTC 2 cut(s) 390, 411
PfeI GAWTC 2 cut(s) 56, 182
PkrI GCNGC 1 cut(s) 390
PscI ACATGT 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 212
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 2 cut(s) 339, 429
PsuI RGATCY 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 65
SapI GCTCTTC 2 cut(s) 390, 411
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 1 cut(s) 85
ScrFI CCNGG 2 cut(s) 214, 350
SduI GDGCHC 3 cut(s) 65, 213, 376
SetI ASST 4 cut(s) 65, 99, 218, 423
SmiMI CAYNNNNRTG 1 cut(s) 176
Sse9I AATT 4 cut(s) 164, 236, 323, 331
SsiI CCGC 1 cut(s) 69
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 212, 348
StyI CCWWGG 2 cut(s) 171, 201
TaqI TCGA 3 cut(s) 59, 93, 106
TasI AATT 4 cut(s) 164, 236, 323, 331
TfiI GAWTC 2 cut(s) 56, 182
TseI GCWGC 1 cut(s) 388
TspGWI ACGGA 1 cut(s) 373
XapI RAATTY 1 cut(s) 164
XceI RCATGY 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.