RLG00000012427

Ethylene-responsive transcription factor 1B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014664
Physical Location & Seq
Forward (+)
18673557 .. 18674318
762 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012427

Sequence Viewer

Length: 762 bp
ATGGAGTCATACTCTCTCTTCCAATTCTCAAACTCTTCTCAATTCTCACCTGAATCCTCCTTCGGTTCGTCGCTAGATTCTTTCTCATGGGACGACGCTTTACTCGATACTTTCAACAACGATATGTTGAACTTTCCACCATTTAACACAAACGAGCCTCAAGACGTGTTGCTGTTCGATGCTGTGGCTGAACAAACGCACTGGTTTGGAGGAGCTGTAAAGGAAGAGGAGGTCACTTCCAATTCCAATGCCTTCCAAGTGTTTGAAGGGAAACCCAGAAAGGAAAAGTCGTACAGAGGGGTCAGGAGGAGGCCTTGGGGGAAATTCGCAGCTGAAATAAGGGACTCGACGAGGCATGGAGTCAGGGTTTGGCTTGGCACTTTCGATAGTGCCGAGGCAGCTGCTTTGGCTTATGACCAAGCTGCATTTGCACTGAGGGGTCCTTTGGCTGTGCTCAATTTTCCGGTTGAAGTTGTGAGGGAGTCACTTCAAGAGATCAAGTGTAGATATGACGAAGGGTGCTCGCCAATTGTGGCACTCAAGAGGAGGCACTCGATGAGAAGAAAGTCTTTGTGCAAAAAGAATAGTTGTAATAAAGAGCAAGAAAAAGTCAAGAGTTCGATTCCTTCCAAAGTAACAAAGAATAAAACGCAAGAGAACGTCGTAACGCAAAATGTGATGGTATTAGAGGATTTGGGTGCTGATTACTTGGAGGAGCTTTTGTATCAAAGCTCATGTGAAAGCGCTAGTTTTTCTTGCTAA

Protein Analysis

254

Amino Acids

28.61

Weight (kDa)

5.58

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 97 - 147 6.6e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014664)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21550
malus_domestica MD05G1198700.v1.1 MD10G1184800.v1.1
prunus_persica Prupe.8G224600_v2.0.a1
pyrus_communis pycom05g18500
rosa_chinensis RchiOBHm_Chr6g0288231
rosa_laevigata RLG00000012427
rosa_multiflora Rmu_co8374589.1_g000001 Rmu_sc0004300.1_g000003 Rmu_sc0009830.1_g000005
rosa_roxburghii Rroxscaffold_7G00180360
rosa_rugosa Rorug06G0194800
rosa_samantha Rh6AG305700 Rh6BG311500 Rh6CG319500 Rh6DG304700
rosa_wichuraiana Rw6G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 745
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 5 cut(s) 115, 130, 266, 470, 491
AjiI CACGTC 1 cut(s) 166
AluBI AGCT 6 cut(s) 215, 332, 401, 422, 718, 732
AluI AGCT 6 cut(s) 215, 332, 401, 422, 718, 732
Alw21I GWGCWC 2 cut(s) 456, 524
Aor51HI AGCGCT 1 cut(s) 745
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 4 cut(s) 329, 398, 401, 422
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 1 cut(s) 746
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 39
AvaII GGWCC 1 cut(s) 440
Bbv12I GWGCWC 2 cut(s) 456, 524
BbvI GCAGC 4 cut(s) 341, 388, 409, 410
BccI CCATC 1 cut(s) 673
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BfaI CTAG 2 cut(s) 74, 747
BfoI RGCGCY 1 cut(s) 747
BisI GCNGC 4 cut(s) 330, 399, 402, 423
BlsI GCNGC 4 cut(s) 331, 400, 403, 424
Bme18I GGWCC 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 441
BmsI GCATC 1 cut(s) 169
BplI GAGNNNNNCTC 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 144, 524
BsaJI CCNNGG 2 cut(s) 314, 393
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 6 cut(s) 201, 231, 351, 381, 707, 737
Bse1I ACTGG 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 314, 393
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 5 cut(s) 225, 242, 322, 559, 728
BseXI GCAGC 4 cut(s) 341, 388, 409, 410
BshFI GGCC 1 cut(s) 313
BsiHKAI GWGCWC 2 cut(s) 456, 524
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 2 cut(s) 104, 356
BsmFI GGGAC 2 cut(s) 104, 356
BsnI GGCC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 456, 524
Bsp143I GATC 1 cut(s) 495
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 441
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 314, 393
BssMI GATC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 314
Bst6I CTCTTC 3 cut(s) 23, 40, 219
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 1 cut(s) 434
BstH2I RGCGCY 1 cut(s) 747
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 1 cut(s) 498
BstMBI GATC 1 cut(s) 495
BstMWI GCNNNNNNNGC 3 cut(s) 398, 407, 428
BstV1I GCAGC 4 cut(s) 341, 388, 409, 410
BsuRI GGCC 1 cut(s) 313
BtrI CACGTC 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 199, 431
Cac8I GCNNGC 1 cut(s) 524
CfoI GCGC 1 cut(s) 746
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 292
CviAII CATG 3 cut(s) 87, 356, 735
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 1 cut(s) 434
DpnI GATC 1 cut(s) 497
DpnII GATC 1 cut(s) 495
Eam1104I CTCTTC 3 cut(s) 23, 40, 219
EarI CTCTTC 3 cut(s) 23, 40, 219
Eco130I CCWWGG 1 cut(s) 314
Eco147I AGGCCT 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 745
EcoO109I RGGNCCY 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 3 cut(s) 90, 359, 738
FaiI YATR 7 cut(s) 10, 88, 125, 357, 414, 510, 736
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FaqI GGGAC 2 cut(s) 104, 356
FatI CATG 3 cut(s) 86, 355, 734
Fnu4HI GCNGC 4 cut(s) 330, 399, 402, 423
Fsp4HI GCNGC 4 cut(s) 330, 399, 402, 423
FspBI CTAG 2 cut(s) 74, 747
GlaI GCGC 1 cut(s) 745
GluI GCNGC 4 cut(s) 330, 399, 402, 423
HaeII RGCGCY 1 cut(s) 747
HaeIII GGCC 1 cut(s) 313
HapII CCGG 1 cut(s) 464
HgaI GACGC 1 cut(s) 104
HhaI GCGC 1 cut(s) 746
Hin1II CATG 3 cut(s) 90, 359, 738
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HinfI GANTC 7 cut(s) 5, 53, 77, 344, 360, 482, 622
HpaII CCGG 1 cut(s) 464
HphI GGTGA 1 cut(s) 39
Hpy188III TCNNGA 5 cut(s) 161, 304, 491, 541, 613
Hpy99I CGWCG 4 cut(s) 73, 98, 352, 665
HpyAV CCTTC 5 cut(s) 70, 260, 262, 509, 636
HpyCH4IV ACGT 2 cut(s) 165, 660
HpyCH4V TGCA 3 cut(s) 425, 431, 576
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 407, 428
HpyF3I CTNAG 1 cut(s) 434
HpySE526I ACGT 2 cut(s) 165, 660
Hsp92II CATG 3 cut(s) 90, 359, 738
HspAI GCGC 1 cut(s) 744
Kzo9I GATC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 212, 715
LpnPI CCDG 6 cut(s) 63, 187, 289, 289, 349, 477
Lsp1109I GCAGC 4 cut(s) 341, 388, 409, 410
LweI GCATC 1 cut(s) 169
MaeI CTAG 2 cut(s) 74, 747
MaeII ACGT 2 cut(s) 165, 660
MaeIII GTNAC 4 cut(s) 232, 483, 634, 664
MalI GATC 1 cut(s) 497
MboI GATC 1 cut(s) 495
MboII GAAGA 4 cut(s) 10, 27, 236, 573
MfeI CAATTG 1 cut(s) 528
MhlI GDGCHC 2 cut(s) 456, 524
MluCI AATT 6 cut(s) 23, 41, 241, 323, 457, 528
MlyI GAGTC 4 cut(s) 14, 338, 369, 491
MseI TTAA 1 cut(s) 144
MspA1I CMGCKG 2 cut(s) 332, 401
MspI CCGG 1 cut(s) 464
MunI CAATTG 1 cut(s) 528
MwoI GCNNNNNNNGC 3 cut(s) 398, 407, 428
NdeII GATC 1 cut(s) 495
NlaIII CATG 3 cut(s) 90, 359, 738
NlaIV GGNNCC 1 cut(s) 441
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 232, 483
PceI AGGCCT 1 cut(s) 313
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 390
PfeI GAWTC 3 cut(s) 53, 77, 622
PkrI GCNGC 4 cut(s) 331, 400, 403, 424
PleI GAGTC 4 cut(s) 13, 338, 368, 490
PpsI GAGTC 4 cut(s) 13, 338, 368, 490
PpuMI RGGWCCY 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 1 cut(s) 440
PspPPI RGGWCCY 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 332, 401
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 4 cut(s) 330, 399, 402, 423
Sau3AI GATC 1 cut(s) 495
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 4 cut(s) 14, 338, 369, 491
SduI GDGCHC 2 cut(s) 456, 524
SfaNI GCATC 1 cut(s) 169
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 159, 539
SmoI CTYRAG 2 cut(s) 159, 539
Sse9I AATT 6 cut(s) 23, 41, 241, 323, 457, 528
SseBI AGGCCT 1 cut(s) 313
SspMI CTAG 2 cut(s) 74, 747
StuI AGGCCT 1 cut(s) 313
StyI CCWWGG 1 cut(s) 314
TaiI ACGT 2 cut(s) 168, 663
TaqI TCGA 6 cut(s) 105, 177, 347, 384, 554, 620
TasI AATT 6 cut(s) 23, 41, 241, 323, 457, 528
TfiI GAWTC 3 cut(s) 53, 77, 622
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 2 cut(s) 206, 438
TseFI GTSAC 2 cut(s) 232, 483
TseI GCWGC 4 cut(s) 329, 398, 401, 422
Tsp45I GTSAC 2 cut(s) 232, 483
TspRI CASTG 2 cut(s) 206, 438
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 323
XspI CTAG 2 cut(s) 74, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.