RLG00000013219

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0020649
Physical Location & Seq
Forward (+)
27901356 .. 27902162
807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013219

Sequence Viewer

Length: 807 bp
ATGAAGTTAATCGGAAAGATCAAAACTATGTGCAAGCGTGATAGAGATATATGGAGATCATATAACGAGGCTTGCAAGAATGATAAGGCTTTCTTAACTGAATTAGGCAAGTTCCTCGAGTCGTATGAGGAATGTATGACTCAACTTAAGGGAATGGAAGAAAAGGGCAGAGGAAGTGATATCAGCTACATGAGGAATATTTTAGAGGAACTGAAGCAGCATTCTGCTGCGGATCAAAGGTTGCACGAAAAGGTTACACATTTGATCAACGAGCGAAAAGTTATGCGTGATGAATTGAGAAGCCAAAATAAGAAGCCTGGCAAGAAGTATGGTTGTGCTCGCGCTTGGAGACGCTGTTTGAGGATGGTCACTTATGTTGTGTTCATTGGTGCTGTTGCTGCTGAATTTGCTTGCACAGTTATGGCCGCAGTTCTGGCTGCTCCACTTGTTGCTCTAGCCATTGCTGCTGTTATTATCCCAACACTAGCCGGACAACACTGGACTTTATCTTGGATTAAAGAATCTCCTTTTGAGAATCAGGACAATGTGATTCGCTTCATGCAACCAAAAATTCTACATCACATTAAGGAGTTGCAAGACACTGAGAGAGGTATCAGGGTTGTTTCAAGAAACATAAACTCACTCTTGGACCTTCTGAACTCTGTAATTGAGGGAGAAGACGACTTCACTATGAAAGAGATCGAGATTCCTCTGAAGAAGCTTAATGATTTGAAGGAAAGAGCAAGGAATTGTAAGTCCATGATATTGAAGGCAAGAGATGAGGTGTCTAAGAAACTGCGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

269

Amino Acids

31.0

Weight (kDa)

9.43

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 55 - 264 1.9e-14 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020649)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013219
rosa_multiflora Rmu_sc0000258.1_g000065
rosa_roxburghii Rroxscaffold_7G00189990 Rroxscaffold_7G00190230
rosa_samantha Rh6BG231400
rosa_wichuraiana Rw6G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 342
AciI CCGC 2 cut(s) 230, 426
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 2 cut(s) 404, 570
AcuI CTGAAG 2 cut(s) 233, 734
AflII CTTAAG 1 cut(s) 146
AgsI TTSAA 3 cut(s) 627, 733, 769
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 2 cut(s) 186, 721
AluI AGCT 2 cut(s) 186, 721
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 1 cut(s) 343
AlwI GGATC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 5 cut(s) 217, 227, 398, 437, 464
ApoI RAATTY 2 cut(s) 404, 570
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 1 cut(s) 649
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 649
BbsI GAAGAC 1 cut(s) 684
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 5 cut(s) 214, 229, 385, 424, 451
BccI CCATC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 343
BfaI CTAG 2 cut(s) 455, 485
BfrI CTTAAG 1 cut(s) 146
BisI GCNGC 6 cut(s) 218, 228, 399, 426, 438, 465
BlsI GCNGC 6 cut(s) 219, 229, 400, 427, 439, 466
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 649
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 649
BmrFI CCNGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bse1I ACTGG 1 cut(s) 503
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 369
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 1 cut(s) 503
BseXI GCAGC 5 cut(s) 214, 229, 385, 424, 451
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 489
BsmAI GTCTC 1 cut(s) 343
BsmBI CGTCTC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 5 cut(s) 18, 56, 232, 264, 699
BspACI CCGC 2 cut(s) 230, 426
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 595
BspFNI CGCG 1 cut(s) 342
BspPI GGATC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 503
BssMI GATC 5 cut(s) 18, 56, 232, 264, 699
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 418
BstAFI CTTAAG 1 cut(s) 146
BstC8I GCNNGC 4 cut(s) 35, 73, 340, 412
BstDEI CTNAG 2 cut(s) 603, 789
BstF5I GGATG 1 cut(s) 369
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 5 cut(s) 21, 59, 235, 267, 702
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 5 cut(s) 18, 56, 232, 264, 699
BstMWI GCNNNNNNNGC 4 cut(s) 398, 407, 434, 464
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 5 cut(s) 214, 229, 385, 424, 451
BstV2I GAAGAC 1 cut(s) 684
BsuRI GGCC 1 cut(s) 425
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 496, 600
Cac8I GCNNGC 4 cut(s) 35, 73, 340, 412
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 1 cut(s) 360
CviAII CATG 3 cut(s) 190, 559, 760
DdeI CTNAG 2 cut(s) 603, 789
DpnI GATC 5 cut(s) 20, 58, 234, 266, 701
DpnII GATC 5 cut(s) 18, 56, 232, 264, 699
EaeI YGGCCR 1 cut(s) 423
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 649
Eco57I CTGAAG 2 cut(s) 233, 734
Eco88I CYCGRG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 316
EcoRV GATATC 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 3 cut(s) 193, 562, 763
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FatI CATG 3 cut(s) 189, 558, 759
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 6 cut(s) 218, 228, 399, 426, 438, 465
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 6 cut(s) 218, 228, 399, 426, 438, 465
FspBI CTAG 2 cut(s) 455, 485
GlaI GCGC 1 cut(s) 343
GluI GCNGC 6 cut(s) 218, 228, 399, 426, 438, 465
HaeIII GGCC 1 cut(s) 425
HapII CCGG 1 cut(s) 489
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 344
Hin1II CATG 3 cut(s) 193, 562, 763
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 6 cut(s) 119, 139, 521, 535, 550, 706
HpaII CCGG 1 cut(s) 489
Hpy188I TCNGA 3 cut(s) 14, 657, 714
Hpy188III TCNNGA 3 cut(s) 539, 627, 703
HpyAV CCTTC 3 cut(s) 662, 727, 763
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4V TGCA 6 cut(s) 33, 75, 244, 414, 562, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 398, 407, 434, 464
HpyF3I CTNAG 2 cut(s) 603, 789
Hsp92II CATG 3 cut(s) 193, 562, 763
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 5 cut(s) 18, 56, 232, 264, 699
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 7 cut(s) 303, 330, 419, 484, 502, 524, 601
Lsp1109I GCAGC 5 cut(s) 214, 229, 385, 424, 451
MaeI CTAG 2 cut(s) 455, 485
MaeIII GTNAC 2 cut(s) 253, 367
MalI GATC 5 cut(s) 20, 58, 234, 266, 701
MboI GATC 5 cut(s) 18, 56, 232, 264, 699
MboII GAAGA 3 cut(s) 170, 689, 727
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 6 cut(s) 101, 293, 404, 570, 666, 748
MlyI GAGTC 2 cut(s) 128, 133
MseI TTAA 6 cut(s) 8, 95, 147, 516, 585, 723
MslI CAYNNNNRTG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 146
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 398, 407, 434, 464
NdeII GATC 5 cut(s) 18, 56, 232, 264, 699
NlaIII CATG 3 cut(s) 193, 562, 763
NmuCI GTSAC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 116
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 521, 535, 550, 706
PkrI GCNGC 6 cut(s) 219, 229, 400, 427, 439, 466
PleI GAGTC 2 cut(s) 127, 133
PpsI GAGTC 2 cut(s) 127, 133
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspPI GGNCC 1 cut(s) 649
PspXI VCTCGAGB 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 6 cut(s) 8, 95, 147, 516, 585, 723
SatI GCNGC 6 cut(s) 218, 228, 399, 426, 438, 465
Sau3AI GATC 5 cut(s) 18, 56, 232, 264, 699
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 2 cut(s) 128, 133
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 1 cut(s) 340
SetI ASST 7 cut(s) 188, 242, 255, 613, 654, 723, 786
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 649
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 116, 146
SmoI CTYRAG 2 cut(s) 116, 146
Sse9I AATT 6 cut(s) 101, 293, 404, 570, 666, 748
SsiI CCGC 2 cut(s) 230, 426
SspI AATATT 1 cut(s) 199
SspMI CTAG 2 cut(s) 455, 485
StyD4I CCNGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 418
TaqI TCGA 2 cut(s) 117, 702
TasI AATT 6 cut(s) 101, 293, 404, 570, 666, 748
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 4 cut(s) 521, 535, 550, 706
Tru1I TTAA 6 cut(s) 8, 95, 147, 516, 585, 723
Tru9I TTAA 6 cut(s) 8, 95, 147, 516, 585, 723
TscAI CASTG 2 cut(s) 503, 607
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 5 cut(s) 217, 227, 398, 437, 464
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 5 cut(s) 17, 306, 373, 547, 707
TspRI CASTG 2 cut(s) 503, 607
Vha464I CTTAAG 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 649
XapI RAATTY 2 cut(s) 404, 570
XhoI CTCGAG 1 cut(s) 116
XspI CTAG 2 cut(s) 455, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.