Rh6BG231400

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0020649
Physical Location & Seq
Reverse (-)
43684694 .. 43686173
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG231400.1

Sequence Viewer

Length: 825 bp
ATGGGAAGAAAGGGAGTGATGAAGTTAATCGGAAAGATTAAAAATATGTGCAAGCGTGATAGAGATATCTGGAGATCATATAACGAGGCTTGCAAGAATGAAGAGGCTTTCTTAACTGAATTAGGCAAGTTCCTCGAGTCGTATGAGAAATGTATGACTCAACTTAAGGGGATGGAAGAAAAAGGCAGAGGAAGTGATATCAGCTACATGAGGAATATTTTAGAGGAACTGAAGCAGCATTCTGCTGTGGATCAAAGGTTGCTCGAAAAGGTTACACGTTTGATCAACGAGCGAGAAGTTATGCATGATGAATTGACAAGCCAAAAAGAGAAGCCTGGCAAGAAGTATGGTTGTGCTCGCACTTGGAGACGCTGTTTGAGGATGGTCACTTATGTTGTGTTCATTGGTGCTGTGGCTGCTGAATTTGCTTGCACAGTTATGGCCGCAGTTCTGGCTGCTCCACTTATTGCTCTAGCCATTGCAGCTGTCATTATCCCAACACTAGCCGGACAACACTGGACTCTATCTTGGATTAAAGAATCTCCTTTTGAGAATCAGGTCAATGTGATTCGCTTCATGCAACCAGAAATTACACATCACATTATGGAGTTGCAAGACACACAGAGAGGTATCAGGGTTGTTTCAAAAGACATAAACTCACTCTTGGACCTTCTGAACTCTGTAATTGAGGGAGAAGACGACTTCACTATGAAAGAGATCGAGAGTCCTCTGGAGCACCTTAATGATTTGAAGGAAAGAGCAAGGAATTGTACGTCCATGACATTGAAGGCAAGAGCTGAGGTGTCTAAGAAACTGCGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

274

Amino Acids

31.52

Weight (kDa)

8.88

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 5 - 271 4.9e-14 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020649)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013219
rosa_multiflora Rmu_sc0000258.1_g000065
rosa_roxburghii Rroxscaffold_7G00189990 Rroxscaffold_7G00190230
rosa_samantha Rh6BG231400
rosa_wichuraiana Rw6G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 444
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 772
AflII CTTAAG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 3 cut(s) 645, 751, 787
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 3 cut(s) 204, 485, 797
AluI AGCT 3 cut(s) 204, 485, 797
Alw21I GWGCWC 2 cut(s) 358, 738
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 4 cut(s) 235, 416, 455, 482
ApoI RAATTY 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 358, 738
BbvCI CCTCAGC 1 cut(s) 798
BbvI GCAGC 4 cut(s) 247, 403, 442, 494
BccI CCATC 2 cut(s) 166, 376
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 2 cut(s) 473, 503
BfrI CTTAAG 1 cut(s) 164
BisI GCNGC 5 cut(s) 236, 417, 444, 456, 483
BlsI GCNGC 5 cut(s) 237, 418, 445, 457, 484
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 667
BmrFI CCNGG 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 702
BpmI CTGGAG 2 cut(s) 91, 752
Bpu10I CCTNAGC 1 cut(s) 798
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 336
BseGI GGATG 2 cut(s) 177, 387
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 1 cut(s) 521
BseXI GCAGC 4 cut(s) 247, 403, 442, 494
BshFI GGCC 1 cut(s) 443
BsiHKAI GWGCWC 2 cut(s) 358, 738
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 361
BsmBI CGTCTC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 358, 738
Bsp143I GATC 4 cut(s) 74, 250, 282, 717
BspACI CCGC 1 cut(s) 444
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 1 cut(s) 790
BspPI GGATC 1 cut(s) 258
BspTI CTTAAG 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 1 cut(s) 521
BssMI GATC 4 cut(s) 74, 250, 282, 717
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 53, 91, 358, 430
BstDEI CTNAG 2 cut(s) 798, 807
BstF5I GGATG 2 cut(s) 177, 387
BstKTI GATC 4 cut(s) 77, 253, 285, 720
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 4 cut(s) 74, 250, 282, 717
BstMWI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstV1I GCAGC 4 cut(s) 247, 403, 442, 494
BstV2I GAAGAC 1 cut(s) 702
BsuRI GGCC 1 cut(s) 443
BtsCI GGATG 2 cut(s) 177, 387
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 4 cut(s) 53, 91, 358, 430
Cfr13I GGNCC 1 cut(s) 667
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 771
CviAII CATG 4 cut(s) 208, 305, 577, 778
CviQI GTAC 1 cut(s) 771
DdeI CTNAG 2 cut(s) 798, 807
DpnI GATC 4 cut(s) 76, 252, 284, 719
DpnII GATC 4 cut(s) 74, 250, 282, 717
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco32I GATATC 2 cut(s) 67, 199
Eco47I GGWCC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 2 cut(s) 67, 199
EcoT22I ATGCAT 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 4 cut(s) 211, 308, 580, 781
FatI CATG 4 cut(s) 207, 304, 576, 777
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 5 cut(s) 236, 417, 444, 456, 483
FokI GGATG 2 cut(s) 184, 394
Fsp4HI GCNGC 5 cut(s) 236, 417, 444, 456, 483
FspBI CTAG 2 cut(s) 473, 503
GluI GCNGC 5 cut(s) 236, 417, 444, 456, 483
GsuI CTGGAG 2 cut(s) 91, 752
HaeIII GGCC 1 cut(s) 443
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 378
Hin1II CATG 4 cut(s) 211, 308, 580, 781
HinfI GANTC 7 cut(s) 137, 157, 520, 539, 553, 568, 724
HpaII CCGG 1 cut(s) 507
Hpy188I TCNGA 2 cut(s) 32, 675
Hpy188III TCNNGA 3 cut(s) 70, 721, 731
HpyAV CCTTC 3 cut(s) 680, 745, 781
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 277, 773
HpyCH4V TGCA 7 cut(s) 51, 93, 304, 432, 482, 580, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
HpyF3I CTNAG 2 cut(s) 798, 807
HpySE526I ACGT 2 cut(s) 277, 773
Hsp92II CATG 4 cut(s) 211, 308, 580, 781
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 4 cut(s) 74, 250, 282, 717
LmnI GCTCC 2 cut(s) 463, 733
Lsp1109I GCAGC 4 cut(s) 247, 403, 442, 494
MaeI CTAG 2 cut(s) 473, 503
MaeII ACGT 2 cut(s) 277, 773
MaeIII GTNAC 2 cut(s) 271, 385
MalI GATC 4 cut(s) 76, 252, 284, 719
MboI GATC 4 cut(s) 74, 250, 282, 717
MboII GAAGA 4 cut(s) 18, 113, 188, 707
MhlI GDGCHC 2 cut(s) 358, 738
MluCI AATT 6 cut(s) 119, 311, 422, 588, 684, 766
MlyI GAGTC 4 cut(s) 146, 151, 514, 733
Mph1103I ATGCAT 1 cut(s) 306
MseI TTAA 6 cut(s) 26, 39, 113, 165, 534, 741
MslI CAYNNNNRTG 2 cut(s) 437, 741
MspA1I CMGCKG 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
NdeII GATC 4 cut(s) 74, 250, 282, 717
NlaIII CATG 4 cut(s) 211, 308, 580, 781
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 134
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 539, 553, 568
PkrI GCNGC 5 cut(s) 237, 418, 445, 457, 484
PleI GAGTC 4 cut(s) 145, 151, 514, 732
PpsI GAGTC 4 cut(s) 145, 151, 514, 732
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspPI GGNCC 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 772
RsaNI GTAC 1 cut(s) 771
RseI CAYNNNNRTG 2 cut(s) 437, 741
SaqAI TTAA 6 cut(s) 26, 39, 113, 165, 534, 741
SatI GCNGC 5 cut(s) 236, 417, 444, 456, 483
Sau3AI GATC 4 cut(s) 74, 250, 282, 717
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 4 cut(s) 146, 151, 514, 733
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 2 cut(s) 358, 738
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 437, 741
SmlI CTYRAG 2 cut(s) 134, 164
SmoI CTYRAG 2 cut(s) 134, 164
Sse9I AATT 6 cut(s) 119, 311, 422, 588, 684, 766
SsiI CCGC 1 cut(s) 444
SspI AATATT 1 cut(s) 217
SspMI CTAG 2 cut(s) 473, 503
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 280, 776
TaqI TCGA 3 cut(s) 135, 264, 720
TasI AATT 6 cut(s) 119, 311, 422, 588, 684, 766
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 3 cut(s) 539, 553, 568
Tru1I TTAA 6 cut(s) 26, 39, 113, 165, 534, 741
Tru9I TTAA 6 cut(s) 26, 39, 113, 165, 534, 741
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 235, 416, 455, 482
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 6 cut(s) 35, 114, 324, 391, 565, 725
TspRI CASTG 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 134
XspI CTAG 2 cut(s) 473, 503
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.