Rroxscaffold_7G00189990

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0020649
Physical Location & Seq
Forward (+)
29906677 .. 29907504
828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189990.1

Sequence Viewer

Length: 828 bp
ATGGGAAGAAAGGGAGTGATGAATAAGTTAATCAGAAAGATTAAAAATATGTGCAAGCGTGATAGAGATATCTGGAGATCATATAACGATGCTTGCAAGAATGATAAGGCCTTCTTAACTGAATTAGGCAAGTTCCTCGAGTCGTACGAGAAATGTATGACTCAACTTAAGGAGATGGAAGATAAAGGCAGTGGAAGTGATATCAGCTACATGAGGAATATTTTAGAGGAACTGAAGCAGCATTCTGCTGCGGATCAAAGGTTGCTCAAAAAGGTTACATATTTGATCAACGAGCGAAAAGTTATGCATGATGAATTGACAGACCGAAAAGAGAAGCCTGGCAAGAAGTATGGTTGCGCTCGCGCTTGGAGACGCTGTTTGAGGATGGTCACTTATTTTGTGTTCATTGGTGCTGTGGCTGCTCAATTTGCTTGCACAGTTGTGGCCGCAGTTCTGGCTGCTCCACTTATTGCACTAGCCATTGCTGCTGCTATTATCCCAACACTAGCCGGACAACACTGGACTCTATCTTGGATTAAAGAATCTCCTTTTGAGAATCAGGACAATGTGATTCGCTTCATGCAACCAGAAATTATACATCACATTAAGGAGTTGCAAGACACTGAGAGAGGTATCAGGGTTGTTTCAAAAAACATATACTCACTCTTGGACCTTCTGAACTCCGTAATTGAGGGAGAAGACGAACTCACTATGAAAGAGATCGAGAGTCCTCTGAAGAACCTTAATGATTTGAAGGAAAGAGCAAGGAATTGTAAGTCCATGATATTGAAGGCAAGAGATGAGTTGTCTAAGAAACTGCGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

275

Amino Acids

31.81

Weight (kDa)

9.38

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 57 - 267 5.8e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020649)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013219
rosa_multiflora Rmu_sc0000258.1_g000065
rosa_roxburghii Rroxscaffold_7G00189990 Rroxscaffold_7G00190230
rosa_samantha Rh6BG231400
rosa_wichuraiana Rw6G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 363
AciI CCGC 2 cut(s) 251, 447
AclWI GGATC 1 cut(s) 261
AcoI YGGCCR 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 254, 755
AfaI GTAC 1 cut(s) 146
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 3 cut(s) 648, 754, 790
AjnI CCWGG 1 cut(s) 337
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 1 cut(s) 207
AluI AGCT 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 108, 444
ApeKI GCWGC 6 cut(s) 238, 248, 419, 458, 485, 488
AspLEI GCGC 2 cut(s) 359, 365
AspS9I GGNCC 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 670
BbsI GAAGAC 1 cut(s) 705
BbvI GCAGC 6 cut(s) 235, 250, 406, 445, 472, 475
BccI CCATC 2 cut(s) 169, 379
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 364
BfaI CTAG 2 cut(s) 476, 506
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 7 cut(s) 239, 249, 420, 447, 459, 486, 489
BlsI GCNGC 7 cut(s) 240, 250, 421, 448, 460, 487, 490
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 670
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bse1I ACTGG 1 cut(s) 524
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 339
BseGI GGATG 1 cut(s) 390
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 1 cut(s) 524
BseXI GCAGC 6 cut(s) 235, 250, 406, 445, 472, 475
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 2 cut(s) 110, 446
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 510
BsiWI CGTACG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 364
BsmBI CGTCTC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 2 cut(s) 110, 446
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 4 cut(s) 77, 253, 285, 720
BspACI CCGC 2 cut(s) 251, 447
BspANI GGCC 2 cut(s) 110, 446
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 363
BspPI GGATC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 1 cut(s) 524
BssMI GATC 4 cut(s) 77, 253, 285, 720
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 439
BstAFI CTTAAG 1 cut(s) 167
BstC8I GCNNGC 4 cut(s) 56, 94, 361, 433
BstDEI CTNAG 2 cut(s) 624, 810
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 363
BstHHI GCGC 2 cut(s) 359, 365
BstKTI GATC 4 cut(s) 80, 256, 288, 723
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 4 cut(s) 77, 253, 285, 720
BstMWI GCNNNNNNNGC 4 cut(s) 419, 428, 455, 485
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 6 cut(s) 235, 250, 406, 445, 472, 475
BstV2I GAAGAC 1 cut(s) 705
BsuRI GGCC 2 cut(s) 110, 446
BtsCI GGATG 1 cut(s) 390
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 3 cut(s) 196, 517, 621
Cac8I GCNNGC 4 cut(s) 56, 94, 361, 433
CfoI GCGC 2 cut(s) 359, 365
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 1 cut(s) 381
Csp6I GTAC 1 cut(s) 145
CviAII CATG 4 cut(s) 211, 308, 580, 781
CviJI RGCY 8 cut(s) 110, 207, 337, 419, 446, 458, 479, 509
CviKI_1 RGCY 8 cut(s) 110, 207, 337, 419, 446, 458, 479, 509
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 624, 810
DpnI GATC 4 cut(s) 79, 255, 287, 722
DpnII GATC 4 cut(s) 77, 253, 285, 720
EaeI YGGCCR 1 cut(s) 444
Eco147I AGGCCT 1 cut(s) 110
Eco32I GATATC 2 cut(s) 70, 202
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 2 cut(s) 254, 755
Eco88I CYCGRG 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 2 cut(s) 70, 202
EcoT22I ATGCAT 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 4 cut(s) 214, 311, 583, 784
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FatI CATG 4 cut(s) 210, 307, 579, 780
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 7 cut(s) 239, 249, 420, 447, 459, 486, 489
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 7 cut(s) 239, 249, 420, 447, 459, 486, 489
FspBI CTAG 2 cut(s) 476, 506
GlaI GCGC 2 cut(s) 358, 364
GluI GCNGC 7 cut(s) 239, 249, 420, 447, 459, 486, 489
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 2 cut(s) 110, 446
HapII CCGG 1 cut(s) 510
HgaI GACGC 1 cut(s) 381
HhaI GCGC 2 cut(s) 359, 365
Hin1II CATG 4 cut(s) 214, 311, 583, 784
Hin6I GCGC 2 cut(s) 357, 363
HinP1I GCGC 2 cut(s) 357, 363
HinfI GANTC 7 cut(s) 140, 160, 523, 542, 556, 571, 727
HpaII CCGG 1 cut(s) 510
Hpy188I TCNGA 3 cut(s) 35, 678, 735
Hpy188III TCNNGA 3 cut(s) 73, 560, 724
HpyAV CCTTC 4 cut(s) 121, 683, 748, 784
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4V TGCA 7 cut(s) 54, 96, 307, 435, 473, 583, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 428, 455, 485
HpyF3I CTNAG 2 cut(s) 624, 810
Hsp92II CATG 4 cut(s) 214, 311, 583, 784
HspAI GCGC 2 cut(s) 357, 363
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 4 cut(s) 77, 253, 285, 720
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 9 cut(s) 58, 324, 351, 440, 505, 523, 545, 600, 622
Lsp1109I GCAGC 6 cut(s) 235, 250, 406, 445, 472, 475
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 476, 506
MaeIII GTNAC 2 cut(s) 274, 388
MalI GATC 4 cut(s) 79, 255, 287, 722
MboI GATC 4 cut(s) 77, 253, 285, 720
MboII GAAGA 4 cut(s) 18, 191, 710, 748
MluCI AATT 6 cut(s) 122, 314, 425, 591, 687, 769
MlyI GAGTC 4 cut(s) 149, 154, 517, 736
MnlI CCTC 7 cut(s) 146, 207, 220, 375, 623, 685, 741
Mph1103I ATGCAT 1 cut(s) 309
MseI TTAA 7 cut(s) 29, 42, 116, 168, 537, 606, 744
MslI CAYNNNNRTG 1 cut(s) 440
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 4 cut(s) 419, 428, 455, 485
NdeII GATC 4 cut(s) 77, 253, 285, 720
NlaIII CATG 4 cut(s) 214, 311, 583, 784
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 137
PceI AGGCCT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 542, 556, 571
Pfl23II CGTACG 1 cut(s) 144
PkrI GCNGC 7 cut(s) 240, 250, 421, 448, 460, 487, 490
PleI GAGTC 4 cut(s) 148, 154, 517, 735
PpsI GAGTC 4 cut(s) 148, 154, 517, 735
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 1 cut(s) 670
PspXI VCTCGAGB 1 cut(s) 137
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 7 cut(s) 29, 42, 116, 168, 537, 606, 744
SatI GCNGC 7 cut(s) 239, 249, 420, 447, 459, 486, 489
Sau3AI GATC 4 cut(s) 77, 253, 285, 720
Sau96I GGNCC 1 cut(s) 670
SchI GAGTC 4 cut(s) 149, 154, 517, 736
ScrFI CCNGG 1 cut(s) 339
SetI ASST 6 cut(s) 209, 263, 276, 634, 675, 744
SfaNI GCATC 1 cut(s) 79
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 137, 167
SmoI CTYRAG 2 cut(s) 137, 167
Sse9I AATT 6 cut(s) 122, 314, 425, 591, 687, 769
SseBI AGGCCT 1 cut(s) 110
SsiI CCGC 2 cut(s) 251, 447
SspI AATATT 1 cut(s) 220
SspMI CTAG 2 cut(s) 476, 506
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 439
TaqI TCGA 2 cut(s) 138, 723
TaqII GACCGA 1 cut(s) 339
TasI AATT 6 cut(s) 122, 314, 425, 591, 687, 769
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 3 cut(s) 542, 556, 571
Tru1I TTAA 7 cut(s) 29, 42, 116, 168, 537, 606, 744
Tru9I TTAA 7 cut(s) 29, 42, 116, 168, 537, 606, 744
TscAI CASTG 3 cut(s) 196, 524, 628
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 6 cut(s) 238, 248, 419, 458, 485, 488
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 5 cut(s) 35, 327, 394, 568, 728
TspGWI ACGGA 1 cut(s) 673
TspRI CASTG 3 cut(s) 196, 524, 628
Vha464I CTTAAG 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 670
XhoI CTCGAG 1 cut(s) 137
XspI CTAG 2 cut(s) 476, 506
Zsp2I ATGCAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.