Rw6G019860

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0020649
Physical Location & Seq
Reverse (-)
39711831 .. 39713373
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019860.1

Sequence Viewer

Length: 834 bp
ATGGGAAGAAAGGGAGTGATGAAGTTAATCGGAAAGATTAAAAATATGTGCAAGCGTGATAGAGATATCTGGAGATCATATAACGAGGCTTGCAAGAATGATGAGGCTTTCTTAACTGAATTAGGCAAGTTCCTCGAGTCGTATGAGAAATGTATGACTCAACTTAAGGGGATGGAAGAAAAAGGCAGAGGAAGTGATATCAGCTACATGAGGAATATTTTAGAGGAACTGAAGCAGCATTCTGCTGTGGATCAAAGGTTGCTCGAAAAGGTTCCACGTTTGATCAACGAGCGAGAAGCTATGCATGATGAATTGACAAGCCTAAAAGAGAAGCCTGGCAAGAAGTATGGTTGTGCTCGCGCTTGGAGACGCTGTTTGAGGATGGTCACTTATGTTGTGTTCATTGGTGCTGTGGCTGCTGAATTTGCTTGCACAGTTATGGCTGCAGTTCTGGCTGCTCCACTTATTGCTCTAGCCATTGCAGCTGTCATTATCCCAACACTAGCCGGACAACACTGGACTCTATCTTGGATTAAAGAATCTCCTTTTGAGAATCAGGTCAATGTGATTCGCTTCATGCAACCAGAAATTACACATCACATTATGGAGTTGCAAGACACACAGAGAGGTATCAGGGTTGTTTCAAAAGACATAAACTCACTCTTGGACCTTCTGAACTCTGTAATTGAGGGAGAAGAGGGAGAAGACGACTTCACTATGAAAGAGATCGAGAGTCCTCTGGAGCACCTTAATGATTTGAAGGAAAGAGCAAGGAATTGTAAGTCCATGATATTGAAGGCAAGAGCTGAGGTGTCTAAGAAACTGCGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

277

Amino Acids

31.78

Weight (kDa)

8.74

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 5 - 274 4.2e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020649)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013219
rosa_multiflora Rmu_sc0000258.1_g000065
rosa_roxburghii Rroxscaffold_7G00189990 Rroxscaffold_7G00190230
rosa_samantha Rh6BG231400
rosa_wichuraiana Rw6G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 360
AclWI GGATC 1 cut(s) 258
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 251
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 3 cut(s) 645, 760, 796
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 4 cut(s) 204, 299, 485, 806
AluI AGCT 4 cut(s) 204, 299, 485, 806
Alw21I GWGCWC 2 cut(s) 358, 747
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 134
ApeKI GCWGC 5 cut(s) 235, 416, 443, 455, 482
ApoI RAATTY 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 711
Bbv12I GWGCWC 2 cut(s) 358, 747
BbvCI CCTCAGC 1 cut(s) 807
BbvI GCAGC 5 cut(s) 247, 403, 430, 442, 494
BccI CCATC 2 cut(s) 166, 376
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 2 cut(s) 473, 503
BfmI CTRYAG 1 cut(s) 444
BfrI CTTAAG 1 cut(s) 164
BisI GCNGC 5 cut(s) 236, 417, 444, 456, 483
BlsI GCNGC 5 cut(s) 237, 418, 445, 457, 484
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 91, 761
Bpu10I CCTNAGC 1 cut(s) 807
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 336
BseGI GGATG 2 cut(s) 177, 387
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 1 cut(s) 521
BseXI GCAGC 5 cut(s) 247, 403, 430, 442, 494
Bsh1236I CGCG 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 358, 747
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 361
BsmBI CGTCTC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 358, 747
Bsp143I GATC 4 cut(s) 74, 250, 282, 726
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 1 cut(s) 258
BspTI CTTAAG 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 1 cut(s) 521
BssMI GATC 4 cut(s) 74, 250, 282, 726
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 690
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 53, 91, 358, 430
BstDEI CTNAG 2 cut(s) 807, 816
BstF5I GGATG 2 cut(s) 177, 387
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 4 cut(s) 77, 253, 285, 729
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 4 cut(s) 74, 250, 282, 726
BstMWI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 444
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 247, 403, 430, 442, 494
BstV2I GAAGAC 1 cut(s) 711
BtsCI GGATG 2 cut(s) 177, 387
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 4 cut(s) 53, 91, 358, 430
CfoI GCGC 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 667
CseI GACGC 1 cut(s) 378
CviAII CATG 4 cut(s) 208, 305, 577, 787
DdeI CTNAG 2 cut(s) 807, 816
DpnI GATC 4 cut(s) 76, 252, 284, 728
DpnII GATC 4 cut(s) 74, 250, 282, 726
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
Eco32I GATATC 2 cut(s) 67, 199
Eco47I GGWCC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 2 cut(s) 67, 199
EcoT22I ATGCAT 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 4 cut(s) 211, 308, 580, 790
FatI CATG 4 cut(s) 207, 304, 576, 786
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 5 cut(s) 236, 417, 444, 456, 483
FokI GGATG 2 cut(s) 184, 394
Fsp4HI GCNGC 5 cut(s) 236, 417, 444, 456, 483
FspBI CTAG 2 cut(s) 473, 503
GlaI GCGC 1 cut(s) 361
GluI GCNGC 5 cut(s) 236, 417, 444, 456, 483
GsuI CTGGAG 2 cut(s) 91, 761
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 378
HhaI GCGC 1 cut(s) 362
Hin1II CATG 4 cut(s) 211, 308, 580, 790
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HinfI GANTC 7 cut(s) 137, 157, 520, 539, 553, 568, 733
HpaII CCGG 1 cut(s) 507
Hpy188I TCNGA 2 cut(s) 32, 675
Hpy188III TCNNGA 3 cut(s) 70, 730, 740
HpyAV CCTTC 3 cut(s) 680, 754, 790
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 8 cut(s) 51, 93, 304, 432, 446, 482, 580, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
HpyF3I CTNAG 2 cut(s) 807, 816
HpySE526I ACGT 1 cut(s) 277
Hsp92II CATG 4 cut(s) 211, 308, 580, 790
HspAI GCGC 1 cut(s) 360
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 4 cut(s) 74, 250, 282, 726
LmnI GCTCC 2 cut(s) 463, 742
Lsp1109I GCAGC 5 cut(s) 247, 403, 430, 442, 494
MaeI CTAG 2 cut(s) 473, 503
MaeII ACGT 1 cut(s) 277
MaeIII GTNAC 1 cut(s) 385
MalI GATC 4 cut(s) 76, 252, 284, 728
MboI GATC 4 cut(s) 74, 250, 282, 726
MboII GAAGA 4 cut(s) 18, 188, 707, 716
MhlI GDGCHC 2 cut(s) 358, 747
MluCI AATT 6 cut(s) 119, 311, 422, 588, 684, 775
MlyI GAGTC 4 cut(s) 146, 151, 514, 742
Mph1103I ATGCAT 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 6 cut(s) 26, 39, 113, 165, 534, 750
MslI CAYNNNNRTG 2 cut(s) 437, 750
MspA1I CMGCKG 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 416, 425, 452, 482
NdeII GATC 4 cut(s) 74, 250, 282, 726
NlaIII CATG 4 cut(s) 211, 308, 580, 790
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 134
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 539, 553, 568
PkrI GCNGC 5 cut(s) 237, 418, 445, 457, 484
PleI GAGTC 4 cut(s) 145, 151, 514, 741
PpsI GAGTC 4 cut(s) 145, 151, 514, 741
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 134
PstI CTGCAG 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 485
RseI CAYNNNNRTG 2 cut(s) 437, 750
SaqAI TTAA 6 cut(s) 26, 39, 113, 165, 534, 750
SatI GCNGC 5 cut(s) 236, 417, 444, 456, 483
Sau3AI GATC 4 cut(s) 74, 250, 282, 726
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 4 cut(s) 146, 151, 514, 742
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 2 cut(s) 358, 747
SfcI CTRYAG 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 437, 750
SmlI CTYRAG 2 cut(s) 134, 164
SmoI CTYRAG 2 cut(s) 134, 164
Sse9I AATT 6 cut(s) 119, 311, 422, 588, 684, 775
SspI AATATT 1 cut(s) 217
SspMI CTAG 2 cut(s) 473, 503
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 280
TaqI TCGA 3 cut(s) 135, 264, 729
TasI AATT 6 cut(s) 119, 311, 422, 588, 684, 775
TfiI GAWTC 3 cut(s) 539, 553, 568
Tru1I TTAA 6 cut(s) 26, 39, 113, 165, 534, 750
Tru9I TTAA 6 cut(s) 26, 39, 113, 165, 534, 750
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 5 cut(s) 235, 416, 443, 455, 482
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 5 cut(s) 35, 324, 391, 565, 734
TspRI CASTG 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 2 cut(s) 473, 503
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.