RLG00000013933
BHLH Family

Transcription factor bHLH3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018728
Physical Location & Seq
Forward (+)
44459180 .. 44459626
447 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013933

Sequence Viewer

Length: 345 bp
ATGTCTGGGAATTTCAGTTCTACAGCTGGCGTTTCAAGTTCAGAGCTTCAGCGGCGGAGGCGTGAGAAGCTTAACCAGCGGTTTTACACCCTTAGAGCTGTTGTTCCTAACATATCGAAGATGGATAAAGCCTCTCTCCTTGGTGATGCCATTACCTACATCAACGATCTCCAGGTGAAGATCAGGGTCATGGAAACTGAGAAGCAGATGGGAAACAGCAGCCAAATGCAGTACCCGGTTCCAGAGATTGATTTCCAGGAACGGCACGAGGATGCTGTTGTGAGGATGAACTGCCCACTGGATTCTCACCCGGTTTCTGACGTCATCAGAACACTTAAGGTTTAG

Protein Analysis

115

Amino Acids

13.03

Weight (kDa)

8.85

Isoelectric Point (pI)

67.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 14 - 58 4.5e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018728)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46510
malus_domestica MD05G1202300.v1.1
rosa_chinensis RchiOBHm_Chr6g0268091
rosa_laevigata RLG00000013933
rosa_samantha Rh6BG153700 Rh6BG162900 Rh6CG155600 Rh6DG138500 Rh6DG148700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
AciI CCGC 3 cut(s) 52, 55, 79
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 233
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 1 cut(s) 36
AjnI CCWGG 2 cut(s) 171, 255
AloI GAACNNNNNNTCC 1 cut(s) 33
AluBI AGCT 4 cut(s) 26, 46, 70, 98
AluI AGCT 4 cut(s) 26, 46, 70, 98
ApeKI GCWGC 1 cut(s) 219
ApoI RAATTY 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 236, 311
AsuHPI GGTGA 3 cut(s) 155, 187, 299
BauI CACGAG 1 cut(s) 266
BbvI GCAGC 1 cut(s) 231
BccI CCATC 2 cut(s) 115, 202
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 173, 257
BcnI CCSGG 2 cut(s) 236, 311
BfmI CTRYAG 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 335
BisI GCNGC 2 cut(s) 53, 220
BlsI GCNGC 2 cut(s) 54, 221
Bme1390I CCNGG 4 cut(s) 173, 236, 257, 311
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 4 cut(s) 173, 236, 257, 311
BmsI GCATC 2 cut(s) 136, 262
BpmI CTGGAG 1 cut(s) 155
BpuMI CCSGG 2 cut(s) 236, 311
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 2 cut(s) 173, 257
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 2 cut(s) 277, 291
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 303
BseXI GCAGC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 236, 311
Bsp143I GATC 2 cut(s) 166, 180
BspACI CCGC 3 cut(s) 52, 55, 79
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 335
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 166, 180
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 266
Bst2UI CCWGG 2 cut(s) 173, 257
BstACI GRCGYC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 335
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 92, 198
BstF5I GGATG 2 cut(s) 277, 291
BstKTI GATC 2 cut(s) 169, 183
BstMBI GATC 2 cut(s) 166, 180
BstMWI GCNNNNNNNGC 4 cut(s) 52, 58, 67, 76
BstNI CCWGG 2 cut(s) 173, 257
BstSCI CCNGG 4 cut(s) 171, 234, 255, 309
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 231
BtsCI GGATG 2 cut(s) 277, 291
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 190
CviJI RGCY 6 cut(s) 26, 46, 70, 98, 131, 222
CviKI_1 RGCY 6 cut(s) 26, 46, 70, 98, 131, 222
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 2 cut(s) 92, 198
DpnI GATC 2 cut(s) 168, 182
DpnII GATC 2 cut(s) 166, 180
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 171, 255
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 193
FaiI YATR 2 cut(s) 113, 191
FatI CATG 1 cut(s) 189
Fnu4HI GCNGC 2 cut(s) 53, 220
FokI GGATG 2 cut(s) 284, 298
Fsp4HI GCNGC 2 cut(s) 53, 220
GluI GCNGC 2 cut(s) 53, 220
GsuI CTGGAG 1 cut(s) 155
HapII CCGG 2 cut(s) 236, 311
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 1 cut(s) 302
HpaII CCGG 2 cut(s) 236, 311
HphI GGTGA 3 cut(s) 155, 187, 299
Hpy188I TCNGA 3 cut(s) 43, 319, 329
Hpy188III TCNNGA 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 58, 67, 76
HpyF3I CTNAG 2 cut(s) 92, 198
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 2 cut(s) 166, 180
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 2 cut(s) 136, 262
MaeII ACGT 1 cut(s) 321
MalI GATC 2 cut(s) 168, 182
MboI GATC 2 cut(s) 166, 180
MboII GAAGA 2 cut(s) 130, 190
MluCI AATT 1 cut(s) 10
MnlI CCTC 4 cut(s) 51, 142, 262, 276
MseI TTAA 2 cut(s) 72, 336
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 3 cut(s) 26, 52, 79
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 2 cut(s) 236, 311
MspR9I CCNGG 4 cut(s) 173, 236, 257, 311
MvaI CCWGG 2 cut(s) 173, 257
MwoI GCNNNNNNNGC 4 cut(s) 52, 58, 67, 76
NciI CCSGG 2 cut(s) 236, 311
NdeII GATC 2 cut(s) 166, 180
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 2 cut(s) 54, 221
Psp6I CCWGG 2 cut(s) 171, 255
PspGI CCWGG 2 cut(s) 171, 255
PspN4I GGNNCC 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 2 cut(s) 72, 336
SatI GCNGC 2 cut(s) 53, 220
Sau3AI GATC 2 cut(s) 166, 180
ScrFI CCNGG 4 cut(s) 173, 236, 257, 311
SetI ASST 8 cut(s) 28, 48, 72, 100, 158, 177, 324, 342
SfaNI GCATC 2 cut(s) 136, 262
SfcI CTRYAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 1 cut(s) 10
SsiI CCGC 3 cut(s) 52, 55, 79
StyD4I CCNGG 4 cut(s) 171, 234, 255, 309
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 116
TasI AATT 1 cut(s) 10
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 2 cut(s) 72, 336
Tru9I TTAA 2 cut(s) 72, 336
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 219
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 335
XapI RAATTY 1 cut(s) 10
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.