Rh6CG155600
BHLH Family

Transcription factor bHLH3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0018728
Physical Location & Seq
Forward (+)
20158254 .. 20158700
447 bp
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UTR
Exon/CDS
Intron
Rh6CG155600.1

Sequence Viewer

Length: 447 bp
ATGTCTGGGAATTTCAGTTCTACAGCTGGCGTTTCAAGTTCAGAGCGGCCTAAAGACAAGTCATTGGCACAAATTGATGAAAGGAAACCTAGAAAGAGAGGGAGAAAGCCTGCCAATGGAAGAGAAGAACCATTGAATCATGTGGAAGCAGAGCGGCAGAGTCGTGAGAAGCTTAACCAGCGGTTTTACACCCTTAGAGCTGTTGTTCCTAACATATCGAAGATGGATAAAGCCTCTCTCCTTTGTGATGCCATTACCTACATCAACGATCTCCAGATGAAGATCAGGGTCATGGAAACTGAGAAGCAGATGGGAAACAGCAGCCAAATGCAGTTCCCGGTTCCAGTGATTGATTTCCAGGAATGGCACGAGGATGCTGTTGTGAGGATGAACTGCCCATTGGATTCTCACCCGGTTTCTGACGTCATCAGAACACTTCAGGTTTAG

Protein Analysis

148

Amino Acids

16.94

Weight (kDa)

8.97

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 92 3.7e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018728)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46510
malus_domestica MD05G1202300.v1.1
rosa_chinensis RchiOBHm_Chr6g0268091
rosa_laevigata RLG00000013933
rosa_samantha Rh6BG153700 Rh6BG162900 Rh6CG155600 Rh6DG138500 Rh6DG148700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
AccBSI CCGCTC 2 cut(s) 46, 154
AciI CCGC 3 cut(s) 46, 154, 181
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 423
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 2 cut(s) 36, 136
AjnI CCWGG 1 cut(s) 357
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AloI GAACNNNNNNTCC 1 cut(s) 33
AluBI AGCT 3 cut(s) 26, 172, 200
AluI AGCT 3 cut(s) 26, 172, 200
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 338, 413
AsuHPI GGTGA 1 cut(s) 401
BauI CACGAG 1 cut(s) 368
BbvI GCAGC 1 cut(s) 333
BccI CCATC 2 cut(s) 217, 304
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 2 cut(s) 338, 413
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 3 cut(s) 47, 155, 322
BlsI GCNGC 3 cut(s) 48, 156, 323
Bme1390I CCNGG 3 cut(s) 338, 359, 413
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 3 cut(s) 338, 359, 413
BmsI GCATC 2 cut(s) 238, 364
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 338, 413
BsaBI GATNNNNATC 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 281
BseBI CCWGG 1 cut(s) 359
BseGI GGATG 2 cut(s) 379, 393
BseJI GATNNNNATC 1 cut(s) 281
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 333
BshFI GGCC 1 cut(s) 49
BsiSI CCGG 2 cut(s) 338, 413
BslI CCNNNNNNNGG 1 cut(s) 116
BsnI GGCC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 268, 282
BspACI CCGC 3 cut(s) 46, 154, 181
BspANI GGCC 1 cut(s) 49
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 342
BsrBI CCGCTC 2 cut(s) 46, 154
BsrI ACTGG 1 cut(s) 344
BssMI GATC 2 cut(s) 268, 282
BssNI GRCGYC 1 cut(s) 423
BssSI CACGAG 1 cut(s) 368
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 28, 111
BstDEI CTNAG 2 cut(s) 194, 300
BstF5I GGATG 2 cut(s) 379, 393
BstKTI GATC 2 cut(s) 271, 285
BstMBI GATC 2 cut(s) 268, 282
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 336, 357, 411
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 333
BsuRI GGCC 1 cut(s) 49
BtsCI GGATG 2 cut(s) 379, 393
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 28, 111
CviAII CATG 2 cut(s) 140, 292
CviJI RGCY 7 cut(s) 26, 49, 109, 172, 200, 233, 324
CviKI_1 RGCY 7 cut(s) 26, 49, 109, 172, 200, 233, 324
DdeI CTNAG 2 cut(s) 194, 300
DpnI GATC 2 cut(s) 270, 284
DpnII GATC 2 cut(s) 268, 282
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 357
FaeI CATG 2 cut(s) 143, 295
FaiI YATR 3 cut(s) 141, 215, 293
FatI CATG 2 cut(s) 139, 291
Fnu4HI GCNGC 3 cut(s) 47, 155, 322
FokI GGATG 2 cut(s) 386, 400
Fsp4HI GCNGC 3 cut(s) 47, 155, 322
FspBI CTAG 1 cut(s) 90
GluI GCNGC 3 cut(s) 47, 155, 322
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 49
HapII CCGG 2 cut(s) 338, 413
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 2 cut(s) 143, 295
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 3 cut(s) 136, 160, 404
HpaII CCGG 2 cut(s) 338, 413
HphI GGTGA 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 43, 421, 431
Hpy188III TCNNGA 2 cut(s) 164, 274
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 2 cut(s) 194, 300
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 2 cut(s) 143, 295
Kzo9I GATC 2 cut(s) 268, 282
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 238, 364
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 423
MalI GATC 2 cut(s) 270, 284
MbiI CCGCTC 2 cut(s) 46, 154
MboI GATC 2 cut(s) 268, 282
MboII GAAGA 4 cut(s) 132, 137, 232, 292
MluCI AATT 2 cut(s) 10, 72
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 4 cut(s) 92, 244, 364, 378
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 2 cut(s) 26, 181
MspI CCGG 2 cut(s) 338, 413
MspR9I CCNGG 3 cut(s) 338, 359, 413
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 178
NciI CCSGG 2 cut(s) 338, 413
NdeII GATC 2 cut(s) 268, 282
NlaIII CATG 2 cut(s) 143, 295
NlaIV GGNNCC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 136, 404
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 3 cut(s) 48, 156, 323
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 3 cut(s) 47, 155, 322
Sau3AI GATC 2 cut(s) 268, 282
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 3 cut(s) 338, 359, 413
SetI ASST 7 cut(s) 28, 91, 174, 202, 260, 426, 444
SfaNI GCATC 2 cut(s) 238, 364
SfcI CTRYAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 372
Sse9I AATT 2 cut(s) 10, 72
SsiI CCGC 3 cut(s) 46, 154, 181
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 3 cut(s) 336, 357, 411
TaiI ACGT 1 cut(s) 426
TaqI TCGA 1 cut(s) 218
TasI AATT 2 cut(s) 10, 72
TauI GCSGC 2 cut(s) 49, 157
TfiI GAWTC 2 cut(s) 136, 404
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 351
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 3 cut(s) 93, 293, 404
TspRI CASTG 1 cut(s) 351
XapI RAATTY 1 cut(s) 10
XspI CTAG 1 cut(s) 90
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.