Rh6BG162900
BHLH Family

Transcription factor bHLH3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018728
Physical Location & Seq
Reverse (-)
26489993 .. 26490580
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG162900.1

Sequence Viewer

Length: 588 bp
ATGTCTGGGAATTTCAGTTCTACAGCTGGCGTTTCAAGTTCAGAGCGGCCTAAAGACAAGTCATTGGCACAAATTGATGAAAGGAAACCTAGAAAGAGAGGGAGAAAGCCTGCCAATGGAAGAGAAGAACCATTGAATCATGTGGAAGCAGAGCGGCAGAGTCGTGAGAAGCTTAACCAGTGGTTTTACACCCTTAGAGCTGTTGTTCCTAACATATCGAAGATGGATAAAGCCTCTCTCCTTGGTGATGCCATTACCTACATCAACGATCTCCAGATGAAGATCAGGGTCATGGAAACTGAGAAGCAGATGGGAAACAGCAGCCAAATGCAGTTCCCGGTTCCAGAGATTGATTTCCAGGAACGGCACGAGGATGCTGTTGTGAGGATGAACTGCCCACTGGATTCTCACCCGGTTTCTGACGTCATCAGAACACTTAAGGAACATAACATTGTAGCTCAAGATACTAATGTTTCTGTAACAGACGATGACACAGTTATTCATACATTCTCCATTCCTACTCAAGGTGGTGATGCCGAGCAGTTAAAGGAGAAGCTGGTGGCCTCTCTTTCAAAATCATCCATGTAA

Protein Analysis

195

Amino Acids

21.97

Weight (kDa)

6.23

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 92 2.6e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018728)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46510
malus_domestica MD05G1202300.v1.1
rosa_chinensis RchiOBHm_Chr6g0268091
rosa_laevigata RLG00000013933
rosa_samantha Rh6BG153700 Rh6BG162900 Rh6CG155600 Rh6DG138500 Rh6DG148700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
AccBSI CCGCTC 2 cut(s) 46, 154
AciI CCGC 2 cut(s) 46, 154
AcsI RAATTY 1 cut(s) 10
AcyI GRCGYC 1 cut(s) 423
AfiI CCNNNNNNNGG 2 cut(s) 116, 524
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 3 cut(s) 36, 136, 573
AjnI CCWGG 1 cut(s) 357
AloI GAACNNNNNNTCC 1 cut(s) 33
AluBI AGCT 5 cut(s) 26, 172, 200, 458, 556
AluI AGCT 5 cut(s) 26, 172, 200, 458, 556
AoxI GGCC 2 cut(s) 47, 561
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 338, 413
AsuHPI GGTGA 3 cut(s) 257, 401, 542
BaeI ACNNNNGTAYC 2 cut(s) 456, 489
BauI CACGAG 1 cut(s) 368
BbvI GCAGC 1 cut(s) 333
BccI CCATC 2 cut(s) 217, 304
BceAI ACGGC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 2 cut(s) 338, 413
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 437
BisI GCNGC 3 cut(s) 47, 155, 322
BlsI GCNGC 3 cut(s) 48, 156, 323
Bme1390I CCNGG 3 cut(s) 338, 359, 413
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 3 cut(s) 338, 359, 413
BmsI GCATC 3 cut(s) 238, 364, 523
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 444, 507
BpuMI CCSGG 2 cut(s) 338, 413
BsaBI GATNNNNATC 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 524
Bse1I ACTGG 2 cut(s) 178, 405
Bse8I GATNNNNATC 1 cut(s) 281
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 3 cut(s) 379, 393, 578
BseJI GATNNNNATC 1 cut(s) 281
BseLI CCNNNNNNNGG 2 cut(s) 116, 524
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 178, 405
BseXI GCAGC 1 cut(s) 333
BshFI GGCC 2 cut(s) 49, 563
BsiSI CCGG 2 cut(s) 338, 413
BslI CCNNNNNNNGG 2 cut(s) 116, 524
BsnI GGCC 2 cut(s) 49, 563
Bsp143I GATC 2 cut(s) 268, 282
BspACI CCGC 2 cut(s) 46, 154
BspANI GGCC 2 cut(s) 49, 563
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 437
BsrBI CCGCTC 2 cut(s) 46, 154
BsrI ACTGG 2 cut(s) 178, 405
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 2 cut(s) 268, 282
BssNI GRCGYC 1 cut(s) 423
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 423
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 28, 111
BstDEI CTNAG 2 cut(s) 194, 300
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 3 cut(s) 379, 393, 578
BstKTI GATC 2 cut(s) 271, 285
BstMBI GATC 2 cut(s) 268, 282
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 336, 357, 411
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 333
BsuRI GGCC 2 cut(s) 49, 563
BtsCI GGATG 3 cut(s) 379, 393, 578
BtsIMutI CAGTG 2 cut(s) 185, 398
Cac8I GCNNGC 2 cut(s) 28, 111
CviAII CATG 3 cut(s) 140, 292, 583
DdeI CTNAG 2 cut(s) 194, 300
DpnI GATC 2 cut(s) 270, 284
DpnII GATC 2 cut(s) 268, 282
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 143, 295, 586
FaiI YATR 6 cut(s) 141, 215, 293, 447, 504, 584
FatI CATG 3 cut(s) 139, 291, 582
Fnu4HI GCNGC 3 cut(s) 47, 155, 322
FokI GGATG 3 cut(s) 386, 400, 565
Fsp4HI GCNGC 3 cut(s) 47, 155, 322
FspBI CTAG 1 cut(s) 90
GluI GCNGC 3 cut(s) 47, 155, 322
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 49, 563
HapII CCGG 2 cut(s) 338, 413
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 3 cut(s) 143, 295, 586
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 3 cut(s) 136, 160, 404
HpaII CCGG 2 cut(s) 338, 413
HphI GGTGA 3 cut(s) 257, 401, 542
Hpy188I TCNGA 3 cut(s) 43, 421, 431
Hpy188III TCNNGA 4 cut(s) 164, 274, 344, 461
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 331
HpyF3I CTNAG 2 cut(s) 194, 300
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 3 cut(s) 143, 295, 586
Kzo9I GATC 2 cut(s) 268, 282
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 3 cut(s) 238, 364, 523
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 270, 284
MbiI CCGCTC 2 cut(s) 46, 154
MboI GATC 2 cut(s) 268, 282
MboII GAAGA 4 cut(s) 132, 137, 232, 292
MluCI AATT 2 cut(s) 10, 72
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 5 cut(s) 92, 244, 364, 378, 574
MseI TTAA 3 cut(s) 174, 438, 545
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 26
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 2 cut(s) 338, 413
MspR9I CCNGG 3 cut(s) 338, 359, 413
MvaI CCWGG 1 cut(s) 359
NciI CCSGG 2 cut(s) 338, 413
NdeII GATC 2 cut(s) 268, 282
NlaIII CATG 3 cut(s) 143, 295, 586
NlaIV GGNNCC 1 cut(s) 342
NmeAIII GCCGAG 1 cut(s) 562
PfeI GAWTC 2 cut(s) 136, 404
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 3 cut(s) 48, 156, 323
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 3 cut(s) 174, 438, 545
SatI GCNGC 3 cut(s) 47, 155, 322
Sau3AI GATC 2 cut(s) 268, 282
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 3 cut(s) 338, 359, 413
SetI ASST 9 cut(s) 28, 91, 174, 202, 260, 426, 460, 529, 558
SfaNI GCATC 3 cut(s) 238, 364, 523
SfcI CTRYAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 3 cut(s) 437, 459, 522
SmoI CTYRAG 3 cut(s) 437, 459, 522
Sse9I AATT 2 cut(s) 10, 72
SsiI CCGC 2 cut(s) 46, 154
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 3 cut(s) 336, 357, 411
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 1 cut(s) 426
TaqI TCGA 1 cut(s) 218
TasI AATT 2 cut(s) 10, 72
TauI GCSGC 2 cut(s) 49, 157
TfiI GAWTC 2 cut(s) 136, 404
Tru1I TTAA 3 cut(s) 174, 438, 545
Tru9I TTAA 3 cut(s) 174, 438, 545
TscAI CASTG 2 cut(s) 185, 405
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 4 cut(s) 93, 293, 404, 491
TspRI CASTG 2 cut(s) 185, 405
Vha464I CTTAAG 1 cut(s) 437
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 1 cut(s) 10
XspI CTAG 1 cut(s) 90
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.