RLG00000014278

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0021542
Physical Location & Seq
Reverse (-)
50269518 .. 50270742
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014278

Sequence Viewer

Length: 873 bp
ATGCAAAAGGGAATGGCTAACCCTATTCTCTCTCGAGCGCTAACCATAACGTCATTCCCGTGTGACGGTGCCAAACACAGCTGCAGCCAAACAATCTCAAGGCGTCTCAGTCTGCCCCTCTCCCTTGATCTACGTGACAGTGTTTTGCTTCAACAATGGTCGGACTGTCCCCTTGGGCTTGGTTGCTTCCCATCTCATCTTATTTGGACGAGGCTGGCTTTTGCCTTCTCTTCCTATTGTAGGTGGCTGCTATATCTTAATCTGAATCAAGGAATGCAGAATTCCTGTCCCGAACTGGGCAAAGGTGATGCCACCGGCTATGGCGGTGGGGTGTTGATCTTGGGTCCTTGGGGTTTGATTTGTTCAGACTGGAGACCAATGCGAAGGATATTGATCCAAATACACCGATTGACATTGGTCATGCTCATATTTGGCCCGAAGGGTGACAAATGGGATGAACATTTTGATCCAAATGGCCATGGAGTGAAGCAGGGTGAAACACGGTGGGCTGGCGAGTATGAAGAGTGCTGGAACAAAACATGGGGCGAGGGCGCCACAATGGTTCAGGGTGGGTCCATAAGTATGGGAAGAACAGCAGTGGGAAATACAAAAGCCATCAGAGATTGCATCACAAATGTCTACAGAGACTTCATCCGAGATGCCTTCAGAGACTTTGTCGGACATGCCTTAAGAGACATCATTGGAGATGCCTTCAGAGACTTCATCGGAAATGACTTTAGAGGCTTCGTCGGAGATGCTTCAGAAATTCCATCGGATATGTCATCAGATAGTCTATCGGAGTTGCCTTCAGAGATTCTGTTGAATAGACAATCAGAGCGTCAGGGGTGGCCTCAGAGATGTCATAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

32.49

Weight (kDa)

7.02

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021542)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014278
rosa_multiflora Rmu_co8385955.1_g000002 Rmu_sc0006767.1_g000006
rosa_samantha Rh6AG130600
rosa_wichuraiana Rw6G011370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 551
AccI GTMKAC 1 cut(s) 639
AciI CCGC 1 cut(s) 324
AclWI GGATC 2 cut(s) 388, 461
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 2 cut(s) 280, 765
AcuI CTGAAG 4 cut(s) 649, 697, 744, 792
AcyI GRCGYC 2 cut(s) 103, 552
AfeI AGCGCT 1 cut(s) 39
AfiI CCNNNNNNNGG 3 cut(s) 65, 240, 296
AflII CTTAAG 1 cut(s) 688
AgsI TTSAA 2 cut(s) 152, 823
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw26I GTCTC 6 cut(s) 110, 367, 639, 663, 687, 711
AlwI GGATC 2 cut(s) 388, 461
Ama87I CYCGRG 1 cut(s) 33
Aor51HI AGCGCT 1 cut(s) 39
AoxI GGCC 3 cut(s) 433, 475, 848
ApeKI GCWGC 3 cut(s) 81, 84, 247
ApoI RAATTY 2 cut(s) 280, 765
AspLEI GCGC 2 cut(s) 40, 554
AspS9I GGNCC 3 cut(s) 344, 434, 573
AsuHPI GGTGA 3 cut(s) 317, 455, 506
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 2 cut(s) 344, 573
BalI TGGCCA 1 cut(s) 477
BanI GGYRCC 2 cut(s) 68, 551
BbvI GCAGC 3 cut(s) 68, 96, 234
BccI CCATC 3 cut(s) 199, 623, 778
BcoDI GTCTC 6 cut(s) 110, 367, 639, 663, 687, 711
BfmI CTRYAG 2 cut(s) 82, 640
BfoI RGCGCY 2 cut(s) 41, 555
BfrI CTTAAG 1 cut(s) 688
BisI GCNGC 3 cut(s) 82, 85, 248
BlsI GCNGC 3 cut(s) 83, 86, 249
Bme18I GGWCC 2 cut(s) 344, 573
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 3 cut(s) 344, 434, 573
BmiI GGNNCC 4 cut(s) 70, 345, 553, 574
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 5 cut(s) 298, 636, 649, 697, 745
BmuI ACTGGG 1 cut(s) 305
BoxI GACNNNNGTC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 82
BsaAI YACGTR 1 cut(s) 134
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 2 cut(s) 103, 552
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 3 cut(s) 172, 347, 478
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 240, 296
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 300, 374
Bse8I GATNNNNATC 1 cut(s) 392
BseDI CCNNGG 3 cut(s) 172, 347, 478
BseGI GGATG 2 cut(s) 460, 651
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 3 cut(s) 65, 240, 296
BseMII CTCAG 2 cut(s) 121, 866
BseNI ACTGG 2 cut(s) 300, 374
BseXI GCAGC 3 cut(s) 68, 96, 234
BshFI GGCC 3 cut(s) 435, 477, 850
BshNI GGYRCC 2 cut(s) 68, 551
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 2 cut(s) 153, 273
BslI CCNNNNNNNGG 3 cut(s) 65, 240, 296
BsmAI GTCTC 6 cut(s) 110, 367, 639, 663, 687, 711
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 153, 273
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 3 cut(s) 435, 477, 850
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 1 cut(s) 33
Bsp143I GATC 4 cut(s) 127, 336, 393, 466
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 1 cut(s) 324
BspANI GGCC 3 cut(s) 435, 477, 850
BspCNI CTCAG 2 cut(s) 120, 865
BspHI TCATGA 1 cut(s) 869
BspLI GGNNCC 4 cut(s) 70, 345, 553, 574
BspMAI CTGCAG 1 cut(s) 86
BspPI GGATC 2 cut(s) 388, 461
BspT107I GGYRCC 2 cut(s) 68, 551
BspTI CTTAAG 1 cut(s) 688
BspTNI GGTCTC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 2 cut(s) 300, 374
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 172, 347, 478
BssMI GATC 4 cut(s) 127, 336, 393, 466
BssNI GRCGYC 2 cut(s) 103, 552
BssT1I CCWWGG 3 cut(s) 172, 347, 478
Bst4CI ACNGT 4 cut(s) 68, 140, 167, 504
Bst6I CTCTTC 2 cut(s) 235, 516
BstACI GRCGYC 2 cut(s) 103, 552
BstAFI CTTAAG 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 216, 511
BstDEI CTNAG 2 cut(s) 107, 852
BstDSI CCRYGG 1 cut(s) 478
BstENI CCTNNNNNAGG 1 cut(s) 238
BstF5I GGATG 2 cut(s) 460, 651
BstH2I RGCGCY 2 cut(s) 41, 555
BstHHI GCGC 2 cut(s) 40, 554
BstKTI GATC 4 cut(s) 130, 339, 396, 469
BstMAI GTCTC 6 cut(s) 110, 367, 639, 663, 687, 711
BstMBI GATC 4 cut(s) 127, 336, 393, 466
BstNSI RCATGY 1 cut(s) 686
BstPAI GACNNNNGTC 1 cut(s) 416
BstSFI CTRYAG 2 cut(s) 82, 640
BstV1I GCAGC 3 cut(s) 68, 96, 234
BstXI CCANNNNNNTGG 1 cut(s) 583
BsuRI GGCC 3 cut(s) 435, 477, 850
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 2 cut(s) 460, 651
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 145, 603
Cac8I GCNNGC 2 cut(s) 216, 511
CciI TCATGA 1 cut(s) 869
CfoI GCGC 2 cut(s) 40, 554
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 344, 434, 573
CseI GACGC 2 cut(s) 92, 827
CviAII CATG 5 cut(s) 421, 479, 540, 683, 870
DdeI CTNAG 2 cut(s) 107, 852
DinI GGCGCC 1 cut(s) 553
DpnI GATC 4 cut(s) 129, 338, 395, 468
DpnII GATC 4 cut(s) 127, 336, 393, 466
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 235, 516
EarI CTCTTC 2 cut(s) 235, 516
Eco130I CCWWGG 3 cut(s) 172, 347, 478
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 344, 573
Eco47III AGCGCT 1 cut(s) 39
Eco57I CTGAAG 4 cut(s) 649, 697, 744, 792
Eco88I CYCGRG 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 280
EcoT14I CCWWGG 3 cut(s) 172, 347, 478
EgeI GGCGCC 1 cut(s) 553
EheI GGCGCC 1 cut(s) 553
ErhI CCWWGG 3 cut(s) 172, 347, 478
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 5 cut(s) 424, 482, 543, 686, 873
FaqI GGGAC 2 cut(s) 153, 273
FatI CATG 5 cut(s) 420, 478, 539, 682, 869
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 3 cut(s) 82, 85, 248
FokI GGATG 2 cut(s) 467, 638
Fsp4HI GCNGC 3 cut(s) 82, 85, 248
GlaI GCGC 2 cut(s) 39, 553
GluI GCNGC 3 cut(s) 82, 85, 248
GsuI CTGGAG 1 cut(s) 391
HaeII RGCGCY 2 cut(s) 41, 555
HaeIII GGCC 3 cut(s) 435, 477, 850
HapII CCGG 1 cut(s) 315
HgaI GACGC 2 cut(s) 92, 827
HhaI GCGC 2 cut(s) 40, 554
Hin1I GRCGYC 2 cut(s) 103, 552
Hin1II CATG 5 cut(s) 424, 482, 543, 686, 873
Hin6I GCGC 2 cut(s) 38, 552
HinP1I GCGC 2 cut(s) 38, 552
HinfI GANTC 2 cut(s) 265, 814
HpaII CCGG 1 cut(s) 315
HphI GGTGA 3 cut(s) 317, 455, 506
Hpy166II GTNNAC 1 cut(s) 640
Hpy188III TCNNGA 3 cut(s) 33, 290, 870
Hpy8I GTNNAC 1 cut(s) 640
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 6 cut(s) 235, 378, 433, 673, 721, 816
HpyCH4III ACNGT 4 cut(s) 68, 140, 167, 504
HpyCH4IV ACGT 2 cut(s) 50, 133
HpyCH4V TGCA 4 cut(s) 4, 84, 277, 627
HpyF3I CTNAG 2 cut(s) 107, 852
HpySE526I ACGT 2 cut(s) 50, 133
Hsp92I GRCGYC 2 cut(s) 103, 552
Hsp92II CATG 5 cut(s) 424, 482, 543, 686, 873
HspAI GCGC 2 cut(s) 38, 552
KasI GGCGCC 1 cut(s) 551
Kzo9I GATC 4 cut(s) 127, 336, 393, 466
Lsp1109I GCAGC 3 cut(s) 68, 96, 234
LweI GCATC 5 cut(s) 298, 636, 649, 697, 745
MaeII ACGT 2 cut(s) 50, 133
MaeIII GTNAC 3 cut(s) 62, 134, 443
MalI GATC 4 cut(s) 129, 338, 395, 468
MboI GATC 4 cut(s) 127, 336, 393, 466
MboII GAAGA 3 cut(s) 222, 533, 600
MlsI TGGCCA 1 cut(s) 477
MluCI AATT 2 cut(s) 280, 765
MluNI TGGCCA 1 cut(s) 477
Mly113I GGCGCC 1 cut(s) 552
MmeI TCCRAC 3 cut(s) 141, 658, 730
MnlI CCTC 5 cut(s) 128, 204, 541, 734, 861
Mox20I TGGCCA 1 cut(s) 477
MscI TGGCCA 1 cut(s) 477
MseI TTAA 2 cut(s) 258, 689
MslI CAYNNNNRTG 2 cut(s) 58, 581
Msp20I TGGCCA 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 81
MspCI CTTAAG 1 cut(s) 688
MspI CCGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 279
NarI GGCGCC 1 cut(s) 552
NcoI CCATGG 1 cut(s) 478
NdeII GATC 4 cut(s) 127, 336, 393, 466
NlaIII CATG 5 cut(s) 424, 482, 543, 686, 873
NlaIV GGNNCC 4 cut(s) 70, 345, 553, 574
NmuCI GTSAC 3 cut(s) 62, 134, 443
NspI RCATGY 1 cut(s) 686
PaeR7I CTCGAG 1 cut(s) 33
PagI TCATGA 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 265, 814
PflFI GACNNNGTC 1 cut(s) 674
PkrI GCNGC 3 cut(s) 83, 86, 249
PluTI GGCGCC 1 cut(s) 555
Ppu21I YACGTR 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 344
PspN4I GGNNCC 4 cut(s) 70, 345, 553, 574
PspPI GGNCC 3 cut(s) 344, 434, 573
PspPPI RGGWCCY 1 cut(s) 344
PstI CTGCAG 1 cut(s) 86
PsyI GACNNNGTC 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 81
RseI CAYNNNNRTG 2 cut(s) 58, 581
SaqAI TTAA 2 cut(s) 258, 689
SatI GCNGC 3 cut(s) 82, 85, 248
Sau3AI GATC 4 cut(s) 127, 336, 393, 466
Sau96I GGNCC 3 cut(s) 344, 434, 573
SetI ASST 5 cut(s) 53, 83, 136, 245, 307
SfaNI GCATC 5 cut(s) 298, 636, 649, 697, 745
SfcI CTRYAG 2 cut(s) 82, 640
SfoI GGCGCC 1 cut(s) 553
Sfr274I CTCGAG 1 cut(s) 33
SinI GGWCC 2 cut(s) 344, 573
SlaI CTCGAG 1 cut(s) 33
SmiMI CAYNNNNRTG 2 cut(s) 58, 581
SmlI CTYRAG 3 cut(s) 33, 97, 688
SmoI CTYRAG 3 cut(s) 33, 97, 688
Sse9I AATT 2 cut(s) 280, 765
SsiI CCGC 1 cut(s) 324
SspDI GGCGCC 1 cut(s) 551
StyI CCWWGG 3 cut(s) 172, 347, 478
TaaI ACNGT 4 cut(s) 68, 140, 167, 504
TaiI ACGT 2 cut(s) 53, 136
TaqI TCGA 1 cut(s) 34
TasI AATT 2 cut(s) 280, 765
TfiI GAWTC 2 cut(s) 265, 814
Tru1I TTAA 2 cut(s) 258, 689
Tru9I TTAA 2 cut(s) 258, 689
TscAI CASTG 2 cut(s) 145, 603
TseFI GTSAC 3 cut(s) 62, 134, 443
TseI GCWGC 3 cut(s) 81, 84, 247
Tsp45I GTSAC 3 cut(s) 62, 134, 443
TspDTI ATGAA 4 cut(s) 471, 534, 640, 712
TspRI CASTG 2 cut(s) 145, 603
Tth111I GACNNNGTC 1 cut(s) 674
Vha464I CTTAAG 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 344, 573
XagI CCTNNNNNAGG 1 cut(s) 238
XapI RAATTY 2 cut(s) 280, 765
XceI RCATGY 1 cut(s) 686
XhoI CTCGAG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.