RLG00000014343

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0021530
Physical Location & Seq
Reverse (-)
51827168 .. 51832890
5723 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014343

Sequence Viewer

Length: 4443 bp
ATGGATGATGATGAGCTTAGAAGAGGAGAGGAAATATCACAACGACTTTTCCGAGCAATTCAAGGGTCGAGGATTTCTCTCATCATCTTCTCCGAAAACTATGCATCCTCAAAGTGGTGTTTGGATGAACTAGTTCATATCCTTGAATGTAGAATGTCAAAGAACCAAATGGTTTGGCCAATCTTTTACAAAGTGGATCCCTCAGATGTAAGAAACCAAAGAGGCAGTTTTGGTAAGGCACTTGCAGAGCATGAGCGCCGATTCAAAGATGACGAGAACAAGGTGTTAAGATGGAGAGCAGCTCTTTCAGAAGCAGCAAATTTATCTGGGTGGCATTTGTCGGAGTCGGACGGGCGTGAATCTAAATTTCTTTTTGGAATCATTGAAGAGATTTCTGCACAACTAATAAATCAGATCCATTTGAATGGGGCAAAGTTTCCAGTTGGGATAGAGTCTCGTGTACAAGATATGCTTCAACTTTTAGAGGTTGGGGAAAATAATGTCCGGATGGTTGGGATGTGCGGAGTTGGTGGAATAGGGAAGACAACAATTGCAAAAGCTGTGTATAATTTGATTGGTCATAAGTTTGATGGAAGTTGCTTTCTGGAAAATGTTAGAGAGAATTCCATGGCACATGGCGGCCTACTCAAACTACGAAGGGTTATTATGTCTAAGATTATAGGGCAGAAAAAATTGAAGGCTACTAAAGTTGATAAAGGAAGGAATGTGACAGAGGAAAGGTTCAGCCATAAGAGAATTCTTTTAGTTCTTGATGATGTGAATCAATTGGAACAGTTAGACACGTTAGCTGGAGTGTCTGATTTGTTTGGTTGCGGCAGCAGAGTAATAATAACAACAAGGAATCAGCTTTTGCTGGCTGCTTATGAAGTCAATGTTATATACAATGTCAGGGAACTAAATCATCATGAAGCTTGTGAGCTCTTCTGTTTAAATGCATTTGGAAAAAATAGAAATATCTATGATGTGAAGTTCCCAATTGATGATGCAGTGAAGTATGCTCGAGGCCTCCCATTAGCTTTGATTGTTTTAGGTTCACATCTCTATGGTAAAAATATCAAGCAATGGCAAGCTGCATTTGATAGTTGCAGAAATGTTCATAATAGAGAGATTCAAGAACTTCTCAAGATAAGTTTCAATGCATTGGAGGGTTTGGTGAAGGAAGCTTTTCTTGACATTGCATGTTTTTTCAAGGGTGCAAATAAGAACCATGTGATACATATCCTTGAAGGTTGTGGCCTGAACCCTAAATATAGTGTTGAAGTACTCATAAAGAAGGCCCTCATATATTTTAATGAAGAGAAGCTTTGTATGCATGACTTGCTAGAAGAAATGGCTAAAAAAATAGTTGTTCAGGAGTCACCCCTACTAGCTTTCCCCGCCAATTTTAATCCAATTATACTTGTTAAACCTGATATGGCTCGCAGCTGCATGTCTGGATTCAAGAGTTTGCAAAATCTGAAATCTTTATGTCTGAAAGGCTGCAATTTCCTGACAGTAACCCCTGACTTCTCTGGATTCCCATCCTTAGAGGACTTGAGTCTAAACTTTTGTTCTAGTTTAGTTGAGGTTCATCCCTCAGTTGGATTCCTTGACAAGCTTGTTAGCCTGAGTCTTGAAGGATGTCAAAACCTCACATTGTTCCCAAGACTAGTCAAGTTGAGATCTCTGCAAGATATCGATCTTAGTGGTTGCAAAAGGCTAGAGAAATTCCCTGAAGTTGTGGGAAAAATGGACTCCTTGACATGGTTGAATCTATCCAACACTGCCATTGAACAGCTGCCTGCAATTGGAAATGATCATACGAATCTAAAAGTATTAAATTTAGCAGGTTGTGAAAAACTTATGACTTTGCCATGCAGCATTTATGACTTGCAACATCTGAACTACCTTTTTGTTCAGGAGCGCGAAAAGCTCGTTCCATTTCCAAATATTGTGAACTCTATAGTGCTTTCAGCTATGTCGAAGAACTTGTTTGACAACTATATATCTTTATCAGAAGCACAAAGAGATGGTCAATGGAATGTGGCCCATCCCGGGCTACGCCTATCTAGTGGCAGAGGGTGCGATTTATTGCTTCCTGAGCTAGTATACTTCAATATATCAAGATGCAGATTATCAGACTGTGATTTCCTCGTTAGTCTTGATTGTTCCTCCACCTTATTAGAACTTGACCTTTCTGGAAACAATTTTGTTAGTCTTCCTGATTGTATCACCAAATTTGTCAACTTGAAAGAGCTGTACTTGAGTGGTTGCAAAAGGCTTCGAGAAATTCAAGAACTTCCACCATATATAGAATGGTTAGATCTGAAAGATTGCGTATCATTGGAAAGAATTTCGAAGCTCTTAAACATTCTGGTTGGTAAAGAATCACAAATGATGGAGTGGATGAACTTGTCTAATTGCCGGAGATTGTGTGACAATCTGGTTCAAATGGCAAACAGAAACGTTACTTTACTGACTGATCAGATCAACAGTCTCATCTCTCTCTTTCTCTCTTTTCAAAACTCTTCATTTGGTGTGGTGTTTCCAGGAAATGAGGTTCCAAAGTGGTTCGACTGGCATATAGATTTCAATGGGCATCAACTGTCTGAACAGTTTCCTATTGAAATTCCCTCAAATTTCAAATGGGAAAACACGGGCTTGGCTATCTTTCTTGTTTTTGAGCCACACATGGATTTCGATGATGTTCTTTGGTCATTCGACATTTCTATTAATGAAGTAACAGTGACCAGGTCTGAGTACTGCAACTGCATTGATTCTACTGAATCGGATCATTTGTGGCTGCAATATATTCCATTCAATACAGTTGATATGGGTCCTTTTGGTTATATGCGTCCATTACCACCTTTTAGGTGTGAAGTTAGTATCCACCAAGGTAGAAGCTGCATTAAAAGCTGTGGAATCCACCTTGTAATGCCGCCAAATGAAGACATTTGTATGCCGGTATCCGCGACTAATAACCTTACAGATCTCCCCAAGTGGAGGTTGCCTGATTGGCCTGGATTTTCATTTGAACCTCCGCCATTGGATTGGATTCCTACAATCCTTCTTAAACTCCGGTCACCACTGGGAGTGGGATTCCAGAGTTGGAAAAATCTGACATCTATAAGTTTTGAAAGCTGCCAATTCATAAAAAAATTCCCGGACATATCTGGATTGCCAAATTTAAAGAATTTGAATCTAAATGACTGTACAAGTCTAGTTGAGTTAGATGATTCTGTTGGAGAATCAGATAATCTTGTTGACTTGAGTCTTTCTGGATGCCATAATCTTATTATGTTTCCAAGAAGAATCACATTGAAATCTGTGAAACATATTAACTTTAAAGGCTGCCAAATGCTGGAGAGCTTGCCCGAAATTGTGCATGAGATGGAATGCTTAACATCGTTGGATCTATCTGGCACTGCCATCAAAGAATTGCCTTCATCATTTGGATATCTTAGAAGCATTGAAGAGTTGGTTTTAAAGGAATGTGAAAAGCTAACCAATCTGCCAAGCAACATTTATGAGTTGCAACATCTATGGTGTCTTGATCTCCAGAACTGCTCAAACCTTGCTGAATTTCCGGAGTGGAGTTCAGAGTCACTTAAAATTAACTCAAAGATTTCACATGATTTGTGGTATCTTAACCTCCGTGGTTGCAAAAGGCTTGTAGAAATTCCTGAGCTGCCACCAAAAGTGGAATGGATAAATCTGGCTGATTGCCTACGATTGAGAAGATTTGCAAAGTTGTCAAACATTCTGGTACATAAAGAGTCAGAAATGATCAAATGTATCACAATGTTCAATTGTCAGAGACTGTGTGACAGTCTGGTTCTTGAAATAGCTGAAAACGGAAATATTATTCTGAATGAGGTCTCTCTCTGCTCTCTCTTTCTCTCTTGTAAGCAGTCTGAATTTGACATTGTGTTTCCTGGAAGTGAAGTTCCAAAGTGGTTCAGCTGTAGGAAAGATTTGATGGAATTAGTTGATAAATGTGAGTTTTCTTTTGAAATCCCTTCAAATTTCAATTTGGGGAACAAGGGATTCGCCATCTGTGCTGCAGCTGCTGCTGTTGAACATATTAGCCCAGAAGAAAAGGAGACTATGCAGCAATGCTCTTTCGCAGCAACAATTGATATCAATGCAGATAGAATTGTCACCCGATCGTTTTGTTTTGAAGCAAAAGACATTGAATCAGCTCATGTGTGGCTTTGCTTTATTCCATTCGTTAAGTTTGTGTATCATCAGTCGCCACCTTTTAAGTGTGGAGTTAGTTTGGAACATATTAGCCAAGGTTCAGTGCGCTGTAAAAGCTTTGGGGTCCATCTGGTAACTCTGCAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGATGAAGATGAAGATGATGAAAGTGAAGATGAAGATGAAGATGAGAAATCAGTTGGTAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1481

Amino Acids

168.12

Weight (kDa)

5.24

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 135 3.8e-40 TIR domain
TIR_2 PF13676 2 - 67 1.7e-07 TIR domain
NB-ARC PF00931 156 - 323 6.9e-17 NB-ARC domain
WHD_ROQ1 PF23282 393 - 459 5e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 542 - 652 1.6e-07 Leucine-rich repeat region
LRR_13 PF23286 560 - 632 8.9e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 705 - 790 5.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_4 PF12799 727 - 768 8.1e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 1107 - 1191 3.4e-08 Leucine-rich repeat region
LRR_13 PF23286 1108 - 1194 9.9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021530)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014343
rosa_multiflora Rmu_sc0000565.1_g000040
rosa_roxburghii Rroxscaffold_3G00228710 Rroxscaffold_7G00204260 Rroxscaffold_7G00204440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1838
AccB7I CCANNNNNTGG 1 cut(s) 3361
AccI GTMKAC 1 cut(s) 2109
AccII CGCG 2 cut(s) 1926, 2972
AccIII TCCGGA 2 cut(s) 504, 3586
AciI CCGC 7 cut(s) 522, 639, 834, 1398, 2939, 2970, 3041
AclI AACGTT 1 cut(s) 2466
AclWI GGATC 5 cut(s) 191, 204, 409, 2799, 3420
AcoI YGGCCR 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 1755
AfaI GTAC 6 cut(s) 462, 1284, 2261, 2762, 3214, 3768
AfiI CCNNNNNNNGG 7 cut(s) 114, 1601, 1764, 1808, 2055, 3003, 3361
AflIII ACRYGT 1 cut(s) 801
AhlI ACTAGT 2 cut(s) 130, 1669
AjnI CCWGG 4 cut(s) 2548, 2750, 3019, 3934
AloI GAACNNNNNNTCC 4 cut(s) 2544, 2576, 3930, 3962
Alw21I GWGCWC 1 cut(s) 942
Alw26I GTCTC 5 cut(s) 459, 2501, 3811, 3883, 4097
AlwI GGATC 5 cut(s) 191, 204, 409, 2799, 3420
AlwNI CAGNNNCTG 2 cut(s) 3820, 4070
Ama87I CYCGRG 2 cut(s) 1020, 2054
Aor13HI TCCGGA 2 cut(s) 504, 3586
AoxI GGCC 7 cut(s) 176, 640, 1024, 1255, 1296, 2046, 3017
ArsI GACNNNNNNTTYG 2 cut(s) 2017, 2049
AseI ATTAAT 1 cut(s) 2733
Asp700I GAANNNNTTC 3 cut(s) 132, 1185, 2616
AspLEI GCGC 3 cut(s) 258, 1926, 4308
AspS9I GGNCC 4 cut(s) 1297, 2047, 2837, 4324
AsuC2I CCSGG 3 cut(s) 2055, 2056, 3164
AsuHPI GGTGA 5 cut(s) 1186, 1371, 2224, 3075, 4153
AsuII TTCGAA 1 cut(s) 2357
AvaI CYCGRG 2 cut(s) 1020, 2054
AvaII GGWCC 2 cut(s) 2837, 4324
BalI TGGCCA 1 cut(s) 178
BamHI GGATCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 942
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 3 cut(s) 548, 2210, 2955
Bbv12I GWGCWC 1 cut(s) 942
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 4 cut(s) 2550, 2752, 3021, 3936
BciVI GTATCC 2 cut(s) 2897, 2977
BclI TGATCA 3 cut(s) 1816, 2482, 3786
BcnI CCSGG 3 cut(s) 2055, 2056, 3164
BcoDI GTCTC 5 cut(s) 459, 2501, 3811, 3883, 4097
BcuI ACTAGT 2 cut(s) 130, 1669
BfaI CTAG 9 cut(s) 131, 1343, 1388, 1575, 1670, 1721, 2070, 2105, 3221
BfmI CTRYAG 3 cut(s) 1962, 3964, 4062
BfoI RGCGCY 1 cut(s) 259
BfuAI ACCTGC 1 cut(s) 1838
BfuI GTATCC 2 cut(s) 2897, 2977
BglI GCCNNNNNGGC 1 cut(s) 3016
BglII AGATCT 3 cut(s) 1682, 2323, 2989
BmcAI AGTACT 2 cut(s) 1284, 2762
Bme1390I CCNGG 7 cut(s) 2055, 2056, 2550, 2752, 3021, 3164, 3936
Bme18I GGWCC 2 cut(s) 2837, 4324
BmeT110I CYCGRG 2 cut(s) 1020, 2054
BmgT120I GGNCC 4 cut(s) 1297, 2047, 2837, 4324
BmiI GGNNCC 4 cut(s) 198, 2562, 2838, 4325
BmrFI CCNGG 7 cut(s) 2055, 2056, 2550, 2752, 3021, 3164, 3936
BmrI ACTGGG 1 cut(s) 3098
BmsI GCATC 5 cut(s) 113, 994, 2117, 2608, 3272
BmuI ACTGGG 1 cut(s) 3098
BoxI GACNNNNGTC 2 cut(s) 1557, 3270
BpiI GAAGAC 3 cut(s) 548, 2210, 2955
BplI GAGNNNNNCTC 4 cut(s) 61, 93, 286, 318
BpmI CTGGAG 3 cut(s) 831, 3383, 3542
Bpu10I CCTNAGC 2 cut(s) 2100, 3684
Bpu14I TTCGAA 1 cut(s) 2357
BpuEI CTTGAG 4 cut(s) 1127, 1576, 2284, 3289
BpuMI CCSGG 3 cut(s) 2055, 2056, 3164
Bsa29I ATCGAT 1 cut(s) 1698
BsaBI GATNNNNATC 4 cut(s) 780, 1238, 1540, 1698
BsaI GGTCTC 1 cut(s) 3883
BsaJI CCNNGG 5 cut(s) 627, 2054, 2893, 3655, 4294
BsaWI WCCGGW 3 cut(s) 504, 3078, 3586
BsaXI ACNNNNNCTCC 2 cut(s) 2419, 2449
Bsc4I CCNNNNNNNGG 7 cut(s) 114, 1601, 1764, 1808, 2055, 3003, 3361
Bse118I RCCGGY 1 cut(s) 2962
Bse1I ACTGG 3 cut(s) 440, 2582, 3093
Bse3DI GCAATG 3 cut(s) 1088, 1194, 4121
Bse8I GATNNNNATC 4 cut(s) 780, 1238, 1540, 1698
BseAI TCCGGA 2 cut(s) 504, 3586
BseBI CCWGG 4 cut(s) 2550, 2752, 3021, 3936
BseCI ATCGAT 1 cut(s) 1698
BseDI CCNNGG 5 cut(s) 627, 2054, 2893, 3655, 4294
BseJI GATNNNNATC 4 cut(s) 780, 1238, 1540, 1698
BseLI CCNNNNNNNGG 7 cut(s) 114, 1601, 1764, 1808, 2055, 3003, 3361
BseMI GCAATG 3 cut(s) 1088, 1194, 4121
BseMII CTCAG 6 cut(s) 216, 1611, 1619, 2091, 2748, 3675
BseNI ACTGG 3 cut(s) 440, 2582, 3093
BseRI GAGGAG 1 cut(s) 39
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 2 cut(s) 1926, 2972
Bsh1285I CGRYCG 1 cut(s) 4169
BshFI GGCC 7 cut(s) 178, 642, 1026, 1257, 1298, 2048, 3019
BshVI ATCGAT 1 cut(s) 1698
BsiEI CGRYCG 1 cut(s) 4169
BsiHKAI GWGCWC 1 cut(s) 942
BsiHKCI CYCGRG 2 cut(s) 1020, 2054
BsiSI CCGG 7 cut(s) 505, 2055, 2425, 2963, 3079, 3164, 3587
BslI CCNNNNNNNGG 7 cut(s) 114, 1601, 1764, 1808, 2055, 3003, 3361
BsmAI GTCTC 5 cut(s) 459, 2501, 3811, 3883, 4097
BsmI GAATGC 1 cut(s) 3401
BsnI GGCC 7 cut(s) 178, 642, 1026, 1257, 1298, 2048, 3019
Bso31I GGTCTC 1 cut(s) 3883
BsoBI CYCGRG 2 cut(s) 1020, 2054
Bsp119I TTCGAA 1 cut(s) 2357
Bsp1286I GDGCHC 1 cut(s) 942
Bsp13I TCCGGA 2 cut(s) 504, 3586
Bsp1407I TGTACA 2 cut(s) 460, 3212
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 7 cut(s) 522, 639, 834, 1398, 2939, 2970, 3041
BspANI GGCC 7 cut(s) 178, 642, 1026, 1257, 1298, 2048, 3019
BspCNI CTCAG 6 cut(s) 215, 1610, 1620, 2092, 2749, 3676
BspDI ATCGAT 1 cut(s) 1698
BspEI TCCGGA 2 cut(s) 504, 3586
BspFNI CGCG 2 cut(s) 1926, 2972
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 4 cut(s) 198, 2562, 2838, 4325
BspMAI CTGCAG 1 cut(s) 4066
BspMI ACCTGC 1 cut(s) 1838
BspPI GGATC 5 cut(s) 191, 204, 409, 2799, 3420
BspQI GCTCTTC 1 cut(s) 947
BspT104I TTCGAA 1 cut(s) 2357
BspTNI GGTCTC 1 cut(s) 3883
BsrDI GCAATG 3 cut(s) 1088, 1194, 4121
BsrFI RCCGGY 1 cut(s) 2962
BsrGI TGTACA 2 cut(s) 460, 3212
BsrI ACTGG 3 cut(s) 440, 2582, 3093
BssAI RCCGGY 1 cut(s) 2962
BssECI CCNNGG 5 cut(s) 627, 2054, 2893, 3655, 4294
BssNAI GTATAC 1 cut(s) 2110
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 3 cut(s) 627, 2893, 4294
Bst1107I GTATAC 1 cut(s) 2110
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 4 cut(s) 2550, 2752, 3021, 3936
Bst6I CTCTTC 6 cut(s) 16, 381, 947, 1311, 2533, 3468
BstAPI GCANNNNNTGC 3 cut(s) 1339, 2082, 4070
BstAUI TGTACA 2 cut(s) 460, 3212
BstBI TTCGAA 1 cut(s) 2357
BstC8I GCNNGC 5 cut(s) 876, 1089, 1441, 1803, 3371
BstDSI CCRYGG 2 cut(s) 627, 3655
BstEII GGTNACC 1 cut(s) 3081
BstFNI CGCG 2 cut(s) 1926, 2972
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 3 cut(s) 258, 1926, 4308
BstMAI GTCTC 5 cut(s) 459, 2501, 3811, 3883, 4097
BstMCI CGRYCG 1 cut(s) 4169
BstNI CCWGG 4 cut(s) 2550, 2752, 3021, 3936
BstNSI RCATGY 2 cut(s) 1203, 1453
BstPAI GACNNNNGTC 2 cut(s) 1557, 3270
BstPI GGTNACC 1 cut(s) 3081
BstSCI CCNGG 7 cut(s) 2053, 2054, 2548, 2750, 3019, 3162, 3934
BstSFI CTRYAG 3 cut(s) 1962, 3964, 4062
BstUI CGCG 2 cut(s) 1926, 2972
BstV2I GAAGAC 3 cut(s) 548, 2210, 2955
BstX2I RGATCY 6 cut(s) 196, 414, 1682, 2323, 2989, 3412
BstXI CCANNNNNNTGG 2 cut(s) 425, 3051
BstYI RGATCY 6 cut(s) 196, 414, 1682, 2323, 2989, 3412
BstZ17I GTATAC 1 cut(s) 2110
Bsu15I ATCGAT 1 cut(s) 1698
BsuI GTATCC 2 cut(s) 2897, 2977
BsuRI GGCC 7 cut(s) 178, 642, 1026, 1257, 1298, 2048, 3019
BsuTUI ATCGAT 1 cut(s) 1698
BtgI CCRYGG 2 cut(s) 627, 3655
BtsI GCAGTG 3 cut(s) 1014, 1782, 3423
BtsIMutI CAGTG 6 cut(s) 1014, 1782, 2751, 3086, 3423, 4308
BveI ACCTGC 1 cut(s) 1838
Cac8I GCNNGC 5 cut(s) 876, 1089, 1441, 1803, 3371
CaiI CAGNNNCTG 2 cut(s) 3820, 4070
CciI TCATGA 1 cut(s) 925
CfoI GCGC 3 cut(s) 258, 1926, 4308
Cfr10I RCCGGY 1 cut(s) 2962
Cfr13I GGNCC 4 cut(s) 1297, 2047, 2837, 4324
Cfr9I CCCGGG 1 cut(s) 2054
ClaI ATCGAT 1 cut(s) 1698
CseI GACGC 1 cut(s) 2843
CsiI ACCWGGT 1 cut(s) 2750
Csp6I GTAC 6 cut(s) 461, 1283, 2260, 2761, 3213, 3767
CviQI GTAC 6 cut(s) 461, 1283, 2260, 2761, 3213, 3767
DraI TTTAAA 4 cut(s) 951, 3189, 3346, 3486
EaeI YGGCCR 1 cut(s) 176
Eam1104I CTCTTC 6 cut(s) 16, 381, 947, 1311, 2533, 3468
EarI CTCTTC 6 cut(s) 16, 381, 947, 1311, 2533, 3468
EciI GGCGGA 1 cut(s) 3030
Ecl136II GAGCTC 1 cut(s) 940
Eco130I CCWWGG 3 cut(s) 627, 2893, 4294
Eco147I AGGCCT 1 cut(s) 1026
Eco24I GRGCYC 1 cut(s) 942
Eco31I GGTCTC 1 cut(s) 3883
Eco32I GATATC 3 cut(s) 1696, 3458, 4141
Eco47I GGWCC 2 cut(s) 2837, 4324
Eco53kI GAGCTC 1 cut(s) 940
Eco57I CTGAAG 1 cut(s) 1755
Eco88I CYCGRG 2 cut(s) 1020, 2054
Eco91I GGTNACC 1 cut(s) 3081
EcoICRI GAGCTC 1 cut(s) 940
EcoO109I RGGNCCY 2 cut(s) 1297, 2837
EcoO65I GGTNACC 1 cut(s) 3081
EcoRI GAATTC 2 cut(s) 622, 756
EcoRII CCWGG 4 cut(s) 2548, 2750, 3019, 3934
EcoRV GATATC 3 cut(s) 1696, 3458, 4141
EcoT14I CCWWGG 3 cut(s) 627, 2893, 4294
EcoT22I ATGCAT 4 cut(s) 106, 958, 1162, 1335
EcoT38I GRGCYC 1 cut(s) 942
ErhI CCWWGG 3 cut(s) 627, 2893, 4294
FalI AAGNNNNNCTT 6 cut(s) 456, 488, 1173, 1205, 1308, 1340
FauI CCCGC 1 cut(s) 1405
FbaI TGATCA 3 cut(s) 1816, 2482, 3786
FblI GTMKAC 1 cut(s) 2109
FriOI GRGCYC 1 cut(s) 942
FspBI CTAG 9 cut(s) 131, 1343, 1388, 1575, 1670, 1721, 2070, 2105, 3221
GlaI GCGC 3 cut(s) 257, 1925, 4307
GsuI CTGGAG 3 cut(s) 831, 3383, 3542
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 7 cut(s) 178, 642, 1026, 1257, 1298, 2048, 3019
HapII CCGG 7 cut(s) 505, 2055, 2425, 2963, 3079, 3164, 3587
HgaI GACGC 1 cut(s) 2843
HhaI GCGC 3 cut(s) 258, 1926, 4308
Hin6I GCGC 3 cut(s) 256, 1924, 4306
HinP1I GCGC 3 cut(s) 256, 1924, 4306
HincII GTYRAC 2 cut(s) 2245, 3265
HindII GTYRAC 2 cut(s) 2245, 3265
HindIII AAGCTT 5 cut(s) 930, 1182, 1322, 1616, 4315
HpaII CCGG 7 cut(s) 505, 2055, 2425, 2963, 3079, 3164, 3587
HphI GGTGA 5 cut(s) 1186, 1371, 2224, 3075, 4153
Hpy166II GTNNAC 6 cut(s) 461, 1055, 1957, 2110, 2245, 3265
Hpy8I GTNNAC 6 cut(s) 461, 1055, 1957, 2110, 2245, 3265
HpyCH4IV ACGT 2 cut(s) 803, 2466
HpySE526I ACGT 2 cut(s) 803, 2466
HspAI GCGC 3 cut(s) 256, 1924, 4306
Kpn2I TCCGGA 2 cut(s) 504, 3586
Ksp22I TGATCA 3 cut(s) 1816, 2482, 3786
LguI GCTCTTC 1 cut(s) 947
LmnI GCTCC 1 cut(s) 1921
LweI GCATC 5 cut(s) 113, 994, 2117, 2608, 3272
MabI ACCWGGT 1 cut(s) 2750
MaeI CTAG 9 cut(s) 131, 1343, 1388, 1575, 1670, 1721, 2070, 2105, 3221
MaeII ACGT 2 cut(s) 803, 2466
MfeI CAATTG 6 cut(s) 549, 785, 996, 1806, 3808, 4134
MflI RGATCY 6 cut(s) 196, 414, 1682, 2323, 2989, 3412
MhlI GDGCHC 1 cut(s) 942
MlsI TGGCCA 1 cut(s) 178
MluNI TGGCCA 1 cut(s) 178
MlyI GAGTC 9 cut(s) 353, 461, 1385, 1567, 1639, 1748, 3280, 3611, 3785
MmeI TCCRAC 7 cut(s) 321, 327, 1582, 1803, 3089, 3223, 3390
Mox20I TGGCCA 1 cut(s) 178
Mph1103I ATGCAT 4 cut(s) 106, 958, 1162, 1335
MroI TCCGGA 2 cut(s) 504, 3586
MroXI GAANNNNTTC 3 cut(s) 132, 1185, 2616
MscI TGGCCA 1 cut(s) 178
MslI CAYNNNNRTG 3 cut(s) 423, 1062, 2957
Msp20I TGGCCA 1 cut(s) 178
MspA1I CMGCKG 4 cut(s) 1446, 1798, 3963, 4067
MspI CCGG 7 cut(s) 505, 2055, 2425, 2963, 3079, 3164, 3587
MspR9I CCNGG 7 cut(s) 2055, 2056, 2550, 2752, 3021, 3164, 3936
MunI CAATTG 6 cut(s) 549, 785, 996, 1806, 3808, 4134
Mva1269I GAATGC 1 cut(s) 3401
MvaI CCWGG 4 cut(s) 2550, 2752, 3021, 3936
MvnI CGCG 2 cut(s) 1926, 2972
NciI CCSGG 3 cut(s) 2055, 2056, 3164
NcoI CCATGG 1 cut(s) 627
NlaIV GGNNCC 4 cut(s) 198, 2562, 2838, 4325
NmuCI GTSAC 8 cut(s) 727, 1377, 2435, 2746, 3081, 3603, 3824, 4159
NsiI ATGCAT 4 cut(s) 106, 958, 1162, 1335
NspI RCATGY 2 cut(s) 1203, 1453
NspV TTCGAA 1 cut(s) 2357
PaeR7I CTCGAG 1 cut(s) 1020
PagI TCATGA 1 cut(s) 925
PceI AGGCCT 1 cut(s) 1026
PciSI GCTCTTC 1 cut(s) 947
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PctI GAATGC 1 cut(s) 3401
PdmI GAANNNNTTC 3 cut(s) 132, 1185, 2616
PflFI GACNNNGTC 1 cut(s) 2752
PflMI CCANNNNNTGG 1 cut(s) 3361
PfoI TCCNGGA 3 cut(s) 2548, 3162, 3934
Ple19I CGATCG 1 cut(s) 4169
PleI GAGTC 9 cut(s) 352, 460, 1384, 1566, 1638, 1748, 3279, 3610, 3784
PpsI GAGTC 9 cut(s) 352, 460, 1384, 1566, 1638, 1748, 3279, 3610, 3784
PpuMI RGGWCCY 1 cut(s) 2837
PshAI GACNNNNGTC 2 cut(s) 1557, 3270
PshBI ATTAAT 1 cut(s) 2733
Psp124BI GAGCTC 1 cut(s) 942
Psp1406I AACGTT 1 cut(s) 2466
Psp5II RGGWCCY 1 cut(s) 2837
Psp6I CCWGG 4 cut(s) 2548, 2750, 3019, 3934
PspEI GGTNACC 1 cut(s) 3081
PspGI CCWGG 4 cut(s) 2548, 2750, 3019, 3934
PspN4I GGNNCC 4 cut(s) 198, 2562, 2838, 4325
PspPI GGNCC 4 cut(s) 1297, 2047, 2837, 4324
PspPPI RGGWCCY 1 cut(s) 2837
PspXI VCTCGAGB 1 cut(s) 1020
PstI CTGCAG 1 cut(s) 4066
PstNI CAGNNNCTG 2 cut(s) 3820, 4070
PsuI RGATCY 6 cut(s) 196, 414, 1682, 2323, 2989, 3412
PsyI GACNNNGTC 1 cut(s) 2752
PvuI CGATCG 1 cut(s) 4169
PvuII CAGCTG 4 cut(s) 1446, 1798, 3963, 4067
RsaI GTAC 6 cut(s) 462, 1284, 2261, 2762, 3214, 3768
RsaNI GTAC 6 cut(s) 461, 1283, 2260, 2761, 3213, 3767
RseI CAYNNNNRTG 3 cut(s) 423, 1062, 2957
SacI GAGCTC 1 cut(s) 942
SapI GCTCTTC 1 cut(s) 947
Sau96I GGNCC 4 cut(s) 1297, 2047, 2837, 4324
ScaI AGTACT 2 cut(s) 1284, 2762
SchI GAGTC 9 cut(s) 353, 461, 1385, 1567, 1639, 1748, 3280, 3611, 3785
ScrFI CCNGG 7 cut(s) 2055, 2056, 2550, 2752, 3021, 3164, 3936
SduI GDGCHC 1 cut(s) 942
SexAI ACCWGGT 1 cut(s) 2750
SfaNI GCATC 5 cut(s) 113, 994, 2117, 2608, 3272
SfcI CTRYAG 3 cut(s) 1962, 3964, 4062
Sfr274I CTCGAG 1 cut(s) 1020
SfuI TTCGAA 1 cut(s) 2357
SinI GGWCC 2 cut(s) 2837, 4324
SlaI CTCGAG 1 cut(s) 1020
SmaI CCCGGG 1 cut(s) 2056
SmiMI CAYNNNNRTG 3 cut(s) 423, 1062, 2957
SmlI CTYRAG 5 cut(s) 1020, 1142, 1555, 2263, 3268
SmoI CTYRAG 5 cut(s) 1020, 1142, 1555, 2263, 3268
SpeI ACTAGT 2 cut(s) 130, 1669
SseBI AGGCCT 1 cut(s) 1026
SsiI CCGC 7 cut(s) 522, 639, 834, 1398, 2939, 2970, 3041
SspI AATATT 2 cut(s) 1951, 3862
SspMI CTAG 9 cut(s) 131, 1343, 1388, 1575, 1670, 1721, 2070, 2105, 3221
SstI GAGCTC 1 cut(s) 942
StuI AGGCCT 1 cut(s) 1026
StyD4I CCNGG 7 cut(s) 2053, 2054, 2548, 2750, 3019, 3162, 3934
StyI CCWWGG 3 cut(s) 627, 2893, 4294
TaiI ACGT 2 cut(s) 806, 2469
TaqI TCGA 9 cut(s) 68, 1021, 1698, 1982, 2284, 2357, 2574, 2700, 2721
TatI WGTACW 5 cut(s) 460, 1282, 2259, 2760, 3212
TauI GCSGC 3 cut(s) 642, 837, 2941
TscAI CASTG 6 cut(s) 1014, 1789, 2751, 3093, 3430, 4308
TseFI GTSAC 8 cut(s) 727, 1377, 2435, 2746, 3081, 3603, 3824, 4159
Tsp45I GTSAC 8 cut(s) 727, 1377, 2435, 2746, 3081, 3603, 3824, 4159
TspGWI ACGGA 2 cut(s) 3644, 3870
TspMI CCCGGG 1 cut(s) 2054
TspRI CASTG 6 cut(s) 1014, 1789, 2751, 3093, 3430, 4308
Tth111I GACNNNGTC 1 cut(s) 2752
Van91I CCANNNNNTGG 1 cut(s) 3361
VpaK11BI GGWCC 2 cut(s) 2837, 4324
VspI ATTAAT 1 cut(s) 2733
XceI RCATGY 2 cut(s) 1203, 1453
XcmI CCANNNNNNNNNTGG 2 cut(s) 2313, 3702
XhoI CTCGAG 1 cut(s) 1020
XmaI CCCGGG 1 cut(s) 2054
XmiI GTMKAC 1 cut(s) 2109
XmnI GAANNNNTTC 3 cut(s) 132, 1185, 2616
XspI CTAG 9 cut(s) 131, 1343, 1388, 1575, 1670, 1721, 2070, 2105, 3221
ZrmI AGTACT 2 cut(s) 1284, 2762
Zsp2I ATGCAT 4 cut(s) 106, 958, 1162, 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.