Rroxscaffold_7G00204440

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0021530
Physical Location & Seq
Reverse (-)
53615844 .. 53637239
21396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204440.1

Sequence Viewer

Length: 4632 bp
ATGTATTTTGAATTTGGATCCTCTTCCTCTGCGTCTCTCACCCATACCGATTTATCTAAACACGATGTGTTTCTGAGTTTCAGAGGCGAGGATACTTGGAACAATTTCACAGGCCATTTGCAAAGGTATTTGGTTCGTAAGGGAATTGACACTTTCATAGATGATGATCAGCTTTCAAGAGGAGAGGAAATATCACCAGCAGTTCTCCGAGCCATTGAACGATCAAAGCTCTCTCTCATCATCTTCTCCGAAAACTATGCATCCTCAAAGTGGTGCTTGGATGAACTAGTTCATATCCTTGAATGTGGAAGATCAAAGTACCAAATGGTTCGGCCAATCTTTCACAAAGTGGATCCCTCGGACGTAAGAAACCAACGAGGTATATTTGGTGAGGCACTTGCAGAGCATGAACGCAGATTCAAAGATGACATGGACAAGGTGTTAAGATGGAGAGCAGCTCTTTCAGAAGCAGCAAATTTGTCTGGATGGCATTTCTTGGACGGCAGAGTTATAATAACAACAAGGAATAAGCATTTGCTGGCTGGTTATGAAGTCAATGTAATATACAATGTCAGGGAACTAAATCATCGTGAAGCTTGTGAGCTCTTCTGTTATAATGCGTTTAAAGGAAATTTATATGATGTGAAATTCCCAGTTGATGATGTAGTACAGTATGCTCGAGGCCTTCCATTAGCTTTGATTGTTTTAGGTTCACATCTCTATGGTAAAAGTATCGAGGAATGGGAAGCTGCATTGGGTAGTTGCAGAAGTGTTCCTAAAAGAGAGATTCAAGAACTTCTCAAAATAAGTATCAATGCATTGGAGGGTTTGGTTAAAGAAGTTTTTCTTAATATTGCATGTTTTTTTAAGGGTGTAGATAAGAACCATGTGATATGTATCCTTGAAGGTTGTGGCCTGAACCCTGACTACAGTATTGAAGTACTCATAAAGAAGGCCCTCATATATGTTAATAAAGAGAAGCTTTGGATGCATGACTTGCTAGAAGAAATTGGTAAAGAAATAGTTGTTCAGGAGTCGCCGAGGGACCCTGGTAAGCGTAGCAGATTGTGGGTTCATGAAGATGTTCTTGAGGTTCTAACCAATAATACTGGAACAAATAAAGTTAAAGGCATCATGGTAAAGTTACCTGAACCAGATGAGATACATTTAAATGCTAAAAGCTTCTCAAAGATGAAAAGTCTCCAACTTCTTATAATTGGTGCTTCATGTTTTGCTGGACACATCGATTATCTCCCCAATGAGTTGAGAGTCCTCGACTGGTCTGACTGTCCCTTACGAGCTTTCCCCTCCAATTTTAATCCAACTAAACTTGTTAAACTTGATATGGCTCGCAGCTGCATGTCTGGACTCAAGAGTTTGCTAAATCTGAAATCTCTAAATCTGAAAGGCTGCAATTTCCTGACAGTGACCCCCGACTTCTCTGGATTCCCAACCTTAGAGGACTTGAATCTAAACTTTTGTTCAAGTTTAGTTGAGGTTCATCCTTCAGTTGGATTCCTTGACAAGCTTGTTAGCCTGAGTCTTGAAGGATGTTACAACCTCATATTGTTCCCAAAACTAGTCAAGTTGAAGTCTCTACGAAGTATCAATCTTAGGGGTTGCAGAAGGCTAGAGAACTTCCCTGAAATTGTGGGAAAGATGGAATCCTTGATATCAATGAATCTATCTGGCACTGCCATTGAACGGCTGCCTTCAGCAATTGGAAATGATCTCATGAATCTAGAAGAATTAGCTTTAGCAGGTTGTGAAAAACTTGAGTATTTGCCATGCAGCATTTATGACTTGCAACATCTGAATTACCTTTTTGTTCAGGAGTGCAAAAAGCTTGTTTCATTTCCAGATATGGTGTACTCTAAAGTGCTTTCAATTGAGTCGAAGATCTCGTATGACAACTATAGATCTTTATCAGAAGCAGAAAGAGATGGTCAAGGGAATGTAGCCCATCCTGGGCTAGGCCTATCTAGTGGCAGAGGGTACAATTTATTGCTTCCTGAGCTAGTATACTTCAATATGTCAAGATGCAGATTATCAGACTGTGATTTCCTTGTTAGTCTTGATTGTTCCTCCACTTTATTAGAACTTGACCTTTCTGGAAGCAATTTTGTTAGTCTTCCCGAATGTATCTCCAAATTTGTCAACTTAAAAGAGCTGTACCTGCGTGGTTGCAAAAGGCTTCAAGAAATTCAAGAACTTCCACCATATATCAAATGGTTAGATTTGAAAGATTGCGTATCATTGGAAAGAATTTCAAAGTTATCAAATATTCTGAATGGTAAAGAGTCAGAAATGATGGAGTGGATGGACTTGTCTAATTGCCGGAGATTGTGTGATACTCTGTTTCAAATGGCAAAGAGAAACTCTGTTTCACTGAGTGATCAGATCAGCAGTCTCTTCTCTCTCTTTCTCTATTTTCAAAACTCTTCATTTGGTGTGGTGCTTCCAGGAAAGGAGATTCCAAAGTGGTTCGGCTGGCATATAGATTTCAACAGGCATCAATTGTCTGAACAGTTTCCTATTGAAATTCCCTCAAATTTCAAATGGGAGAACACAGGATTGGCTATCTCTGTTGCTTTTGATTCACACAGGGATTTCGGTGATTTTCTTTGGTCATTCGATATTTCTATTAATGAAGTTAGGGTGGCAGGGTCTGACTGTTGCATCGATTCAACTGAATCGGCTCATGTATGGCTGCAATATATTCCATTCCATACAGTTGATATGGGTCCTTTTGATTATATGCGTCCATTACCGCCTTTTAGGTGTCAAGTTAGTATCCACCAAGGTAGCAGCAGTTCAGTGAGTTTCAAAAGCTGTGGGGTTCACCTTGTAATGCCACCAAATGAAGATGTCTGTATGACGGTATCTGCTACTGATAACCTCACGGATCTCCCCAAGTGGAGGGTCCTTGATTGGCCTGGAATTGTACCTCTACCATTGGAGTGGAATCCTACAACGCTTATTAAACTCATTTTGTCTGGAAGTATTGTGTTACCACTGTGTTGGAAAAATCTGACATCTATAAGTTTTGAAAGCTGCCAATTCATAAAAAAATTCCCAGACATATCTGGATTGCCAAATTTAGAGAGTCTGAATCTAAATGACTGTACAAGTTTAGTTGAGTTAGATGATTCTGTTGGAGAATTAGATAATCTTGTTGAGTTGAGTCTTTCTGGATGCCATCATCTGACTACGTTTCCAAGAAGACTCACCTTGAAATCTGTGAAACGTATCAACTTTAAAGGCTGCAGCATGCTAGAAATTTTACCTGAAATTGTGCATGAGATGGATTGCATAACATGGTTGGATCTATCTGGCACCGCCATCAAAGAATTGCCTTCATCAAATAGATATCTTAGAAGCCTTGAAGAGTTGGTCTTAAAGGAATGTGAAAACCTGACCAATCTGCCGAGCAACATTTATGAGTTGCAACATCTATGGTGTCTTGATCTCCAGAACTGCTCAAAACTTGTTGCGTTTCCAGAGTGGAGTTCAGAGTCACTTCAAATTAACTCGAAGATTTCACATGACTTGTGGTATCTTAACCTCCGTGGTTGCACGAGGCTTGAAGAAATTCCTGAACTCCCACCAAAAGTGGAATGGATAAATGTGGCTCATTGCCTACAATTGAAAAGATTTGCAAAGTTTTCAAACATCCTGGCACATAAAGAATCAGAAATGATCAAATGTATCACAATGTTTAATTGTCAGAGACTGTGTGACAGTCTGGCTCTTGAAATGGCTGAAAATGGAAATATTCTTCTGAATGAGGTGACTCTCTGCTCTCTTTTTCTCTCTTGTAAGCAGTCTGAATTTGACATCGTGTTTCCTGGAAGTGAAGTTCCAAAGTGGTTCAGCGGTAGGAAAGATTTGATGGAATTAATTGATAAATGTGAGTTTTCTTTTGAAATCCCTCCAAATTTCAATTTGGGGAACAAAGGATTTGCCATCTGTGCTGCAGCTGCTGCTGTTGAAAATATTAACCCAGAAGAAAAGGAGATTTTGCAGCCCTGCTCTTTCACAGCAACAATTGATATCAATGCGGACAGAACTGTCACCCGATCATTTTATTTTGAAGCAAAAGAAATTGAATTAGCTCATGTGTGGCTTTGCTATATTCCATTCGTTAATTCACTCAAGAGAGTAGATAAAGATGCTCTACCTCCACGAGCTTCAGAAGCTCACGATTTCTACATTGATAAAGAGGAGGGCTTCATTATTCGTTTCCAGGTCATGGGGCAAGCCACTGATGCAGAGAAAGCAATGGCCGTGTCTGAGTTTCGTGATTTCAAGCGCAGGAACGTTGTATCTGTTATGATTAGAAAATCTGCCCGCGGCATCGGGCGGGTTCTCATGCTAGTTTCTGATTTTGATCGTGGACCGCTAGACCGTAGGGGTTGCATCCATCAAGCTCTGGGATACGAAGGTTGGCCTGATAACAAGTGGGAAAGTGGAGATATGAATGGTAAGGATGAAAGCCTGCAAAAAGGTTATCGGAGCAGCTTGAGGCTCTGGAAAGATCCGCAGAGGCAATATCTTTTGGTGGAATCAACCACGCTTTCCTATTGTGTTCTGATACAGATATGGGGAGCAGTTGGAAGGGAGGGAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1543

Amino Acids

175.58

Weight (kDa)

5.62

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 165 2.6e-47 TIR domain
TIR_2 PF13676 23 - 117 2.9e-12 TIR domain
WHD_ROQ1 PF23282 279 - 345 4.7e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 512 - 621 6.3e-09 Leucine-rich repeat region
LRR_13 PF23286 530 - 622 9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 675 - 762 1.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1107 - 1163 1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021530)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014343
rosa_multiflora Rmu_sc0000565.1_g000040
rosa_roxburghii Rroxscaffold_3G00228710 Rroxscaffold_7G00204260 Rroxscaffold_7G00204440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 512, 615, 1214
AasI GACNNNNNNGTC 1 cut(s) 4070
Acc36I ACCTGC 2 cut(s) 1751, 2184
AccB1I GGYRCC 1 cut(s) 3335
AccB7I CCANNNNNTGG 1 cut(s) 4252
AccI GTMKAC 1 cut(s) 2022
AccII CGCG 1 cut(s) 4353
AciI CCGC 9 cut(s) 2771, 3339, 3876, 4061, 4351, 4353, 4363, 4400, 4541
AclI AACGTT 1 cut(s) 4320
AclWI GGATC 7 cut(s) 12, 25, 347, 360, 2913, 3333, 4532
AcoI YGGCCR 2 cut(s) 332, 4284
AcuI CTGAAG 3 cut(s) 1491, 1698, 4176
AdeI CACNNNGTG 3 cut(s) 67, 349, 2393
AfaI GTAC 8 cut(s) 320, 669, 942, 1871, 1997, 2174, 2946, 3127
AfiI CCNNNNNNNGG 7 cut(s) 270, 1511, 1704, 1968, 1973, 2919, 4252
AhlI ACTAGT 2 cut(s) 286, 1579
AjnI CCWGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
AjuI GAANNNNNNNTTGG 2 cut(s) 2558, 2590
AloI GAACNNNNNNTCC 2 cut(s) 3843, 3875
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 6 cut(s) 39, 1205, 1599, 2414, 3724, 4620
AlwI GGATC 7 cut(s) 12, 25, 347, 360, 2913, 3333, 4532
AlwNI CAGNNNCTG 3 cut(s) 2669, 3733, 3983
Ama87I CYCGRG 1 cut(s) 678
AoxI GGCC 9 cut(s) 112, 332, 682, 913, 954, 1975, 2933, 4284, 4448
AseI ATTAAT 2 cut(s) 2646, 3899
Asp700I GAANNNNTTC 6 cut(s) 104, 288, 843, 1083, 2529, 3591
AspLEI GCGC 1 cut(s) 4314
AspS9I GGNCC 5 cut(s) 955, 1045, 2744, 2923, 4397
AsuHPI GGTGA 8 cut(s) 31, 186, 401, 2627, 2834, 3220, 3802, 4066
AvaI CYCGRG 1 cut(s) 678
AvaII GGWCC 4 cut(s) 1045, 2744, 2923, 4397
BaeI ACNNNNGTAYC 2 cut(s) 4429, 4462
BamHI GGATCC 2 cut(s) 17, 352
BanI GGYRCC 1 cut(s) 3335
BanII GRGCYC 1 cut(s) 606
BauI CACGAG 2 cut(s) 3577, 4185
BbsI GAAGAC 2 cut(s) 2123, 3229
Bbv12I GWGCWC 1 cut(s) 606
BceAI ACGGC 3 cut(s) 517, 1721, 4271
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
BciVI GTATCC 4 cut(s) 85, 908, 2804, 4430
BclI TGATCA 3 cut(s) 166, 2395, 3699
BcoDI GTCTC 6 cut(s) 39, 1205, 1599, 2414, 3724, 4620
BcuI ACTAGT 2 cut(s) 286, 1579
BfmI CTRYAG 4 cut(s) 928, 1915, 3265, 3975
BfuAI ACCTGC 2 cut(s) 1751, 2184
BfuI GTATCC 4 cut(s) 85, 908, 2804, 4430
BglII AGATCT 2 cut(s) 1899, 1919
BmcAI AGTACT 1 cut(s) 942
Bme1390I CCNGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
Bme18I GGWCC 4 cut(s) 1045, 2744, 2923, 4397
BmeT110I CYCGRG 1 cut(s) 678
BmgT120I GGNCC 5 cut(s) 955, 1045, 2744, 2923, 4397
BmiI GGNNCC 7 cut(s) 19, 354, 1046, 1047, 2745, 2924, 3337
BmrFI CCNGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
BmrI ACTGGG 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 647
BpiI GAAGAC 2 cut(s) 2123, 3229
BplI GAGNNNNNCTC 2 cut(s) 442, 474
BpmI CTGGAG 1 cut(s) 3455
Bpu10I CCTNAGC 1 cut(s) 2013
BpuEI CTTGAG 5 cut(s) 1109, 1355, 1796, 4139, 4543
Bsa29I ATCGAT 2 cut(s) 1245, 2682
BsaBI GATNNNNATC 4 cut(s) 165, 896, 1924, 4389
BsaJI CCNNGG 7 cut(s) 357, 1040, 1048, 1967, 2800, 3568, 4351
BsaXI ACNNNNNCTCC 2 cut(s) 2332, 2362
Bsc4I CCNNNNNNNGG 7 cut(s) 270, 1511, 1704, 1968, 1973, 2919, 4252
Bse1I ACTGG 3 cut(s) 653, 1114, 1283
Bse3DI GCAATG 2 cut(s) 3634, 4287
Bse8I GATNNNNATC 4 cut(s) 165, 896, 1924, 4389
BseBI CCWGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
BseCI ATCGAT 2 cut(s) 1245, 2682
BseDI CCNNGG 7 cut(s) 357, 1040, 1048, 1967, 2800, 3568, 4351
BseJI GATNNNNATC 4 cut(s) 165, 896, 1924, 4389
BseLI CCNNNNNNNGG 7 cut(s) 270, 1511, 1704, 1968, 1973, 2919, 4252
BseMI GCAATG 2 cut(s) 3634, 4287
BseMII CTCAG 5 cut(s) 65, 1529, 2004, 2381, 4284
BseNI ACTGG 3 cut(s) 653, 1114, 1283
BseRI GAGGAG 2 cut(s) 195, 4238
Bsh1236I CGCG 1 cut(s) 4353
BshFI GGCC 9 cut(s) 114, 334, 684, 915, 956, 1977, 2935, 4286, 4450
BshNI GGYRCC 1 cut(s) 3335
BshVI ATCGAT 2 cut(s) 1245, 2682
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 1 cut(s) 678
BsiSI CCGG 1 cut(s) 2338
BslFI GGGAC 2 cut(s) 1058, 1275
BslI CCNNNNNNNGG 7 cut(s) 270, 1511, 1704, 1968, 1973, 2919, 4252
BsmAI GTCTC 6 cut(s) 39, 1205, 1599, 2414, 3724, 4620
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 2 cut(s) 1058, 1275
BsnI GGCC 9 cut(s) 114, 334, 684, 915, 956, 1977, 2935, 4286, 4450
BsoBI CYCGRG 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 606
Bsp1407I TGTACA 1 cut(s) 3125
BspACI CCGC 9 cut(s) 2771, 3339, 3876, 4061, 4351, 4353, 4363, 4400, 4541
BspANI GGCC 9 cut(s) 114, 334, 684, 915, 956, 1977, 2935, 4286, 4450
BspCNI CTCAG 5 cut(s) 66, 1530, 2005, 2382, 4285
BspDI ATCGAT 2 cut(s) 1245, 2682
BspFNI CGCG 1 cut(s) 4353
BspHI TCATGA 2 cut(s) 1075, 1734
BspLI GGNNCC 7 cut(s) 19, 354, 1046, 1047, 2745, 2924, 3337
BspMAI CTGCAG 2 cut(s) 3269, 3979
BspMI ACCTGC 2 cut(s) 1751, 2184
BspPI GGATC 7 cut(s) 12, 25, 347, 360, 2913, 3333, 4532
BspQI GCTCTTC 1 cut(s) 611
BspT107I GGYRCC 1 cut(s) 3335
BsrDI GCAATG 2 cut(s) 3634, 4287
BsrGI TGTACA 1 cut(s) 3125
BsrI ACTGG 3 cut(s) 653, 1114, 1283
BssECI CCNNGG 7 cut(s) 357, 1040, 1048, 1967, 2800, 3568, 4351
BssNAI GTATAC 1 cut(s) 2023
BssSI CACGAG 2 cut(s) 3577, 4185
BssT1I CCWWGG 1 cut(s) 2800
Bst1107I GTATAC 1 cut(s) 2023
Bst2BI CACGAG 2 cut(s) 3577, 4185
Bst2UI CCWGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
Bst6I CTCTTC 5 cut(s) 28, 611, 2417, 2446, 3381
BstAPI GCANNNNNTGC 2 cut(s) 997, 3983
BstAUI TGTACA 1 cut(s) 3125
BstC8I GCNNGC 7 cut(s) 540, 1351, 2492, 3272, 4260, 4351, 4499
BstDEI CTNAG 8 cut(s) 74, 1456, 1538, 1613, 2013, 2390, 3374, 4293
BstDSI CCRYGG 2 cut(s) 3568, 4351
BstENI CCTNNNNNAGG 1 cut(s) 1971
BstFNI CGCG 1 cut(s) 4353
BstHHI GCGC 1 cut(s) 4314
BstMAI GTCTC 6 cut(s) 39, 1205, 1599, 2414, 3724, 4620
BstNI CCWGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
BstNSI RCATGY 3 cut(s) 861, 1363, 3274
BstSCI CCNGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
BstSFI CTRYAG 4 cut(s) 928, 1915, 3265, 3975
BstUI CGCG 1 cut(s) 4353
BstV2I GAAGAC 2 cut(s) 2123, 3229
BstX2I RGATCY 7 cut(s) 17, 352, 1899, 1919, 2905, 3325, 4537
BstXI CCANNNNNNTGG 2 cut(s) 2961, 3021
BstYI RGATCY 7 cut(s) 17, 352, 1899, 1919, 2905, 3325, 4537
BstZ17I GTATAC 1 cut(s) 2023
Bsu15I ATCGAT 2 cut(s) 1245, 2682
BsuI GTATCC 4 cut(s) 85, 908, 2804, 4430
BsuRI GGCC 9 cut(s) 114, 334, 684, 915, 956, 1977, 2935, 4286, 4450
BsuTUI ATCGAT 2 cut(s) 1245, 2682
BtgI CCRYGG 2 cut(s) 3568, 4351
BtsI GCAGTG 1 cut(s) 1692
BtsIMutI CAGTG 6 cut(s) 1431, 1692, 2387, 2823, 3014, 4263
BveI ACCTGC 2 cut(s) 1751, 2184
Cac8I GCNNGC 7 cut(s) 540, 1351, 2492, 3272, 4260, 4351, 4499
CaiI CAGNNNCTG 3 cut(s) 2669, 3733, 3983
CciI TCATGA 2 cut(s) 1075, 1734
CfoI GCGC 1 cut(s) 4314
Cfr13I GGNCC 5 cut(s) 955, 1045, 2744, 2923, 4397
Cfr42I CCGCGG 1 cut(s) 4354
ClaI ATCGAT 2 cut(s) 1245, 2682
CseI GACGC 2 cut(s) 21, 2750
Csp6I GTAC 8 cut(s) 319, 668, 941, 1870, 1996, 2173, 2945, 3126
CspCI CAANNNNNGTGG 2 cut(s) 2815, 2850
CviQI GTAC 8 cut(s) 319, 668, 941, 1870, 1996, 2173, 2945, 3126
DdeI CTNAG 8 cut(s) 74, 1456, 1538, 1613, 2013, 2390, 3374, 4293
DraI TTTAAA 3 cut(s) 625, 1170, 3259
DraIII CACNNNGTG 3 cut(s) 67, 349, 2393
DrdI GACNNNNNNGTC 1 cut(s) 4070
DseDI GACNNNNNNGTC 1 cut(s) 4070
EaeI YGGCCR 2 cut(s) 332, 4284
Eam1104I CTCTTC 5 cut(s) 28, 611, 2417, 2446, 3381
EarI CTCTTC 5 cut(s) 28, 611, 2417, 2446, 3381
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 2800
Eco147I AGGCCT 2 cut(s) 684, 1977
Eco24I GRGCYC 1 cut(s) 606
Eco32I GATATC 3 cut(s) 1674, 3371, 4054
Eco47I GGWCC 4 cut(s) 1045, 2744, 2923, 4397
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 3 cut(s) 1491, 1698, 4176
Eco88I CYCGRG 1 cut(s) 678
EcoICRI GAGCTC 1 cut(s) 604
EcoNI CCTNNNNNAGG 1 cut(s) 1971
EcoO109I RGGNCCY 4 cut(s) 955, 1045, 2744, 2923
EcoRII CCWGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
EcoRV GATATC 3 cut(s) 1674, 3371, 4054
EcoT14I CCWWGG 1 cut(s) 2800
EcoT22I ATGCAT 3 cut(s) 262, 820, 993
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 2800
Esp3I CGTCTC 1 cut(s) 39
FalI AAGNNNNNCTT 8 cut(s) 260, 292, 831, 863, 966, 998, 1071, 1103
FaqI GGGAC 2 cut(s) 1058, 1275
FauI CCCGC 2 cut(s) 4356, 4358
FbaI TGATCA 3 cut(s) 166, 2395, 3699
FblI GTMKAC 1 cut(s) 2022
FriOI GRGCYC 1 cut(s) 606
GlaI GCGC 1 cut(s) 4313
GsuI CTGGAG 1 cut(s) 3455
HaeIII GGCC 9 cut(s) 114, 334, 684, 915, 956, 1977, 2935, 4286, 4450
HapII CCGG 1 cut(s) 2338
HgaI GACGC 2 cut(s) 21, 2750
HhaI GCGC 1 cut(s) 4314
Hin6I GCGC 1 cut(s) 4312
HinP1I GCGC 1 cut(s) 4312
HincII GTYRAC 1 cut(s) 2158
HindII GTYRAC 1 cut(s) 2158
HindIII AAGCTT 5 cut(s) 594, 980, 1180, 1526, 1844
HpaII CCGG 1 cut(s) 2338
HphI GGTGA 8 cut(s) 31, 186, 401, 2627, 2834, 3220, 3802, 4066
Hpy166II GTNNAC 6 cut(s) 713, 1870, 2023, 2158, 2842, 4397
Hpy8I GTNNAC 6 cut(s) 713, 1870, 2023, 2158, 2842, 4397
HpyCH4IV ACGT 4 cut(s) 363, 3212, 3247, 4320
HpyF3I CTNAG 8 cut(s) 74, 1456, 1538, 1613, 2013, 2390, 3374, 4293
HpySE526I ACGT 4 cut(s) 363, 3212, 3247, 4320
HspAI GCGC 1 cut(s) 4312
KflI GGGWCCC 1 cut(s) 1045
Ksp22I TGATCA 3 cut(s) 166, 2395, 3699
KspI CCGCGG 1 cut(s) 4354
LguI GCTCTTC 1 cut(s) 611
LmnI GCTCC 2 cut(s) 4515, 4607
MaeII ACGT 4 cut(s) 363, 3212, 3247, 4320
MaeIII GTNAC 8 cut(s) 1143, 1426, 1553, 3009, 3516, 3737, 3790, 4072
MfeI CAATTG 5 cut(s) 1719, 1887, 2516, 3644, 4047
MflI RGATCY 7 cut(s) 17, 352, 1899, 1919, 2905, 3325, 4537
MhlI GDGCHC 1 cut(s) 606
MmeI TCCRAC 7 cut(s) 1228, 1346, 1492, 3002, 3136, 3303, 4594
Mph1103I ATGCAT 3 cut(s) 262, 820, 993
MroXI GAANNNNTTC 6 cut(s) 104, 288, 843, 1083, 2529, 3591
MslI CAYNNNNRTG 4 cut(s) 720, 1080, 1170, 2959
MspA1I CMGCKG 4 cut(s) 1356, 3876, 3980, 4353
MspI CCGG 1 cut(s) 2338
MspR9I CCNGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
MunI CAATTG 5 cut(s) 1719, 1887, 2516, 3644, 4047
MvaI CCWGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
MvnI CGCG 1 cut(s) 4353
NlaIV GGNNCC 7 cut(s) 19, 354, 1046, 1047, 2745, 2924, 3337
NmeAIII GCCGAG 2 cut(s) 1065, 3453
NmuCI GTSAC 5 cut(s) 1426, 3516, 3737, 3790, 4072
NsiI ATGCAT 3 cut(s) 262, 820, 993
NspI RCATGY 3 cut(s) 861, 1363, 3274
PaeI GCATGC 1 cut(s) 3274
PaeR7I CTCGAG 1 cut(s) 678
PagI TCATGA 2 cut(s) 1075, 1734
PceI AGGCCT 2 cut(s) 684, 1977
PciSI GCTCTTC 1 cut(s) 611
PcsI WCGNNNNNNNCGW 1 cut(s) 1901
PdmI GAANNNNTTC 6 cut(s) 104, 288, 843, 1083, 2529, 3591
PflMI CCANNNNNTGG 1 cut(s) 4252
PfoI TCCNGGA 2 cut(s) 2461, 3847
PpuMI RGGWCCY 3 cut(s) 1045, 2744, 2923
PshBI ATTAAT 2 cut(s) 2646, 3899
PsiI TTATAA 3 cut(s) 512, 615, 1214
Psp124BI GAGCTC 1 cut(s) 606
Psp1406I AACGTT 1 cut(s) 4320
Psp5II RGGWCCY 3 cut(s) 1045, 2744, 2923
Psp6I CCWGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
PspGI CCWGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
PspN4I GGNNCC 7 cut(s) 19, 354, 1046, 1047, 2745, 2924, 3337
PspPI GGNCC 5 cut(s) 955, 1045, 2744, 2923, 4397
PspPPI RGGWCCY 3 cut(s) 1045, 2744, 2923
PspXI VCTCGAGB 1 cut(s) 678
PsrI GAACNNNNNNTAC 4 cut(s) 911, 943, 2797, 2829
PstI CTGCAG 2 cut(s) 3269, 3979
PstNI CAGNNNCTG 3 cut(s) 2669, 3733, 3983
PsuI RGATCY 7 cut(s) 17, 352, 1899, 1919, 2905, 3325, 4537
PvuII CAGCTG 2 cut(s) 1356, 3980
RsaI GTAC 8 cut(s) 320, 669, 942, 1871, 1997, 2174, 2946, 3127
RsaNI GTAC 8 cut(s) 319, 668, 941, 1870, 1996, 2173, 2945, 3126
RseI CAYNNNNRTG 4 cut(s) 720, 1080, 1170, 2959
SacI GAGCTC 1 cut(s) 606
SacII CCGCGG 1 cut(s) 4354
SapI GCTCTTC 1 cut(s) 611
Sau96I GGNCC 5 cut(s) 955, 1045, 2744, 2923, 4397
ScaI AGTACT 1 cut(s) 942
ScrFI CCNGG 7 cut(s) 1050, 1968, 2463, 2937, 3677, 3849, 4247
SduI GDGCHC 1 cut(s) 606
SfcI CTRYAG 4 cut(s) 928, 1915, 3265, 3975
Sfr274I CTCGAG 1 cut(s) 678
Sfr303I CCGCGG 1 cut(s) 4354
SgrBI CCGCGG 1 cut(s) 4354
SinI GGWCC 4 cut(s) 1045, 2744, 2923, 4397
SlaI CTCGAG 1 cut(s) 678
SmiI ATTTAAAT 1 cut(s) 1170
SmiMI CAYNNNNRTG 4 cut(s) 720, 1080, 1170, 2959
SmlI CTYRAG 6 cut(s) 678, 1088, 1370, 1775, 4154, 4522
SmoI CTYRAG 6 cut(s) 678, 1088, 1370, 1775, 4154, 4522
SpeI ACTAGT 2 cut(s) 286, 1579
SphI GCATGC 1 cut(s) 3274
SseBI AGGCCT 2 cut(s) 684, 1977
SsiI CCGC 9 cut(s) 2771, 3339, 3876, 4061, 4351, 4353, 4363, 4400, 4541
SspI AATATT 4 cut(s) 853, 2284, 3775, 3997
SstI GAGCTC 1 cut(s) 606
StuI AGGCCT 2 cut(s) 684, 1977
StyD4I CCNGG 7 cut(s) 1048, 1966, 2461, 2935, 3675, 3847, 4245
StyI CCWWGG 1 cut(s) 2800
SwaI ATTTAAAT 1 cut(s) 1170
TaiI ACGT 4 cut(s) 366, 3215, 3250, 4323
TaqI TCGA 8 cut(s) 679, 735, 1245, 1275, 1895, 2634, 2682, 3533
TatI WGTACW 4 cut(s) 667, 940, 1869, 3125
TauI GCSGC 1 cut(s) 4356
TscAI CASTG 6 cut(s) 1431, 1699, 2394, 2823, 3021, 4270
TseFI GTSAC 5 cut(s) 1426, 3516, 3737, 3790, 4072
Tsp45I GTSAC 5 cut(s) 1426, 3516, 3737, 3790, 4072
TspGWI ACGGA 2 cut(s) 2918, 3557
TspRI CASTG 6 cut(s) 1431, 1699, 2394, 2823, 3021, 4270
Van91I CCANNNNNTGG 1 cut(s) 4252
VpaK11BI GGWCC 4 cut(s) 1045, 2744, 2923, 4397
VspI ATTAAT 2 cut(s) 2646, 3899
XagI CCTNNNNNAGG 1 cut(s) 1971
XbaI TCTAGA 1 cut(s) 1741
XceI RCATGY 3 cut(s) 861, 1363, 3274
XcmI CCANNNNNNNNNTGG 2 cut(s) 2226, 3615
XhoI CTCGAG 1 cut(s) 678
XmiI GTMKAC 1 cut(s) 2022
XmnI GAANNNNTTC 6 cut(s) 104, 288, 843, 1083, 2529, 3591
ZrmI AGTACT 1 cut(s) 942
Zsp2I ATGCAT 3 cut(s) 262, 820, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.