Rroxscaffold_7G00204260

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0021530
Physical Location & Seq
Reverse (-)
53458086 .. 53466373
8288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204260.1

Sequence Viewer

Length: 3291 bp
ATGTACACGCACGATGTGTTTCTGAGTTTTAGAGGCGTGGACACTCGCAACAGTTTCACAGGCCATTTGTTCACCGCACTGGCTCAGAAGGGGATTAGCACCTTCATGGATGATGATGAGCTTAGAAGACGAGAGGAAATATCACAACGACTTGTCCGAGCAATTCAAGGGTCGAGGATTTCTCTCATTGTATTCTCCCAAAACTATGCATCCTCAAAGTGGTGTTTGGATGAACTAGTTCGTATCCTTGAATGTAGAATGTCAAAGAACCAAATGGTTTGGCCAATCTTTTACAAAGTGGATCCCTCAGATGTAAGAAACCAAAGAGGCAGTTTTGGTAAGGCACTTGCAGAGCATGAGCGCCGATTCAAAGATGACGAGAACAAGGTGTTAAGATGGAGAGCAGCTCTTTCAGAAGCAGCCAATTTATCTGGGTGGCATTTGTCGGAGTCGGACGGCTGCATGTCTGGATTCAAGAGTTTGCAAAATCTGAAATCTTTATGTCTGAAAGGCTGCAATTTCCTGACAGTAACCCCTGACTTCTCTGGATTCCCATCCTTAGAGGACTTGAATCTAAACTTTTGTTCAAGTTTAGTTGAGGTTCATCCCTCAGTTGGATTCCTTGACAAGCTTGTTAGCCTGAGTCTTGAAGGATGTCAAAACCTCACATTGTTCCCAAGACTAGTCAACTTGAGATCTCTGCGAGATATCGATCTTAGTGGTTGTAAAAGGCTAGAGAACTTCCCTGAAGTTGTGGGAAAAATGGACTCCTTGACATGGATGAATCTATCCAACACTGCCATTGAACAGCTGCCTGCAATTGGAAATGATCTTATGAATCTAAAAGAATTAAATTTAGCAGGTTGTGAAAAACTTATGACTTTGCCATGCTGCATTTATGACTTGCAACATCTGAACTACCTTTTTGTTCAGGGGTGCGAAAAGCTCGTTCCATTTCCAAATATTGTGAACTCTATAGTGCTTTCAGCTATGTCGAAGATCTCGCTTGACAACTATAGATCTTTATCAGAAGCAGAAAGAGATGGTCAAGGGAATGTGGCCCATCCCGGGCTACGCCTATCTAGTGGCAGAGGGTGCGATCTATTGCTTCCTAAGCTAGTATACTTCAATATATCAAGATGCAGATTATCAGACTGTGATTTCCTCGTTAGTCTTGATTGTTCCTCCACCTTATTAGAACTTGACCTTTCTGGAAACAATTTTGTTAGTCTTCCTGATTGTATCACCAAATTTGTCAACTTGAAAGAGCTGTACTTGAGTGGTTGCAAAAGGCTTCGAGAAATTCAAGAACTTCCACCATATATAGAATGGTTAGATCTGAAAGATTGCGTATCATTGGAAAGAATTTCAAAGTTCTCAAACATTCTGATTGGTAAAGAATCACAAATGATGGAGTGGATGAACTTGTCTAATTGCCGGAGATTGTGTGACAATCTGGTTCAAATGGCAAACAGAAACGGTATTTTACTGACTGATCAGATCAACAGTCTCATCTCTCTCTTTCTCTCTTTTCAAAACTCTTCATTTGGTGTGCTGTTTCCAGGAAATGAGGTTCCAAAGTGGTTCGACTGGCATATAGATTTCAATGGGCATCAACTGTCTGAACAGTTTCCTATTGAAATTCCCTCAAATTTCAAATGGGAGAACATGGGCTTGGCTATCTTTCTTGTTTTTGAGCCACACATGGATTTCGATGATGTTCTTTGGTCATTCGACATTTCTATTAATGAAGTAACAGTTACCAGGTCTGAGTACTGCATTGATTCTACTGAATCGGATCATGTGTGGCTGGAATATATTCCATTCAATACAGTTGATATGGGTCCTTTTGATTATATGCGTCCATTACCACCTTTTAGATGTGAAGTTAGTATCCACCAAGGTAGAAGCTGCATTAAAGGCTGTGGAATCCACCTTGTAATGCCACCAAATGAAGACGTTTGTATGCCGGTATCCGCGACTAATAACCTTACAGATCTCCCCAAGTGGAGGTTGCCTGATTGGCCTGGATTTTCATTTGAACCTCTGCCATTGGATTGGATTCCTACAACCCTTCTTAAACTCCGGTCACCACTGGGAGTGGGATTCCAGTGGAGTTCAGAGTCACTTCAAATTAACTCAAAGATTTCACATGACTTGTGGTATCTTAACCTCCGTGGTTGCAAAAGGCTTGTAGAAATTCCTGAGCTGCCACCAAAAGTGGAATGGATAAATCTGGCTGATTGCCTACGATTGAGAAGATTTGCAAAGTTGTCAAACATTCTGGTACATAAAGAGTCAGAAATGATCAAATGTATCACAATGTTCAATTGTCAGAGACTGTGTGACAGTCTGGCTCTTGAAATGGCTGAAAACGGAAATATTCTTCTGAATGAGGTCTCTCTCTGCTCTCTCTTTCTCTCTTGTAAGCAGTCTGAATTTGACATCGTGTTTCCTGGAAGTGAAGTTCCAAAGTGGTTCAGCTGTAGGAAAGATTTGATGGAATTAGTTGATAAATGTGAGTTTTCTTTTGAAGTCCCTGCAAATTTCAGTTTGGGGAACGAGGGATTCGCCATCTGTGCTGCAGCTGCTGCTGTTGAACATATTAGCCCAGAAGAAAAGGAGACTATGCAGCAATGCTCTTTCGCAGCAACAATTGATATCAATGCAGATAGAATTGTCACCCGATCGTTTTGTTTTGAAGCTAAAGACATTGAATCAGCTCATGTGTGGCTTTGCTTTATTCCATTTGTTAAGTTTGCGTATCATCAGTCGCCACCTTTTAAGTGTGGAGTTAGTTTGGAACATACTAGCCAAGGGGACCCCTTTGTCAGAGTGGTTCAGAATGCTCAGGATTCAACTCATGATGATGGTGATAATGAAGAGAAGAACTTTCAACCTGCACTCCCAGATTCAGTCTTCAAATCCCAAGATGATATTGATAATGTTGTTTATGGAGCCCCTTCTTATCCTAGCCCATGGAAGAGGGGCAGAGACGATCTGATTGAAGAGGATAATGATCCTGGAACGGTGAAACCTCAAAAGACTCTTGAAGATGAATCTCTTCAAAGAGCTTGTCTTCATGAATATTTTAGCCTGGAATTGCAGAGGGTATTCGACCTTTATGGGAAAGACTACTTCAATGTCATGAATGCTTCAGAGATCACTGGCAAACTTGGTCTCCACACTATTGTCAACACTAATGGTGAGATTGGGGAAGAATGGTGTGGTGAATGTTTTGGAATTGGGTCCTCTCTCAAGACTTTGAGAAGGAAGGATTTGAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1096

Amino Acids

124.77

Weight (kDa)

5.06

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 4 - 150 1.6e-51 TIR domain
TIR_2 PF13676 6 - 102 1.1e-13 TIR domain
LRR_14 PF23598 213 - 338 2.7e-09 Leucine-rich repeat region
LRR_13 PF23286 231 - 316 3.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 375 - 460 4.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_4 PF12799 398 - 439 5.7e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021530)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014343
rosa_multiflora Rmu_sc0000565.1_g000040
rosa_roxburghii Rroxscaffold_3G00228710 Rroxscaffold_7G00204260 Rroxscaffold_7G00204440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2828
Acc36I ACCTGC 2 cut(s) 851, 2908
AccI GTMKAC 1 cut(s) 1122
AccII CGCG 1 cut(s) 1979
AciI CCGC 2 cut(s) 75, 1977
AclWI GGATC 4 cut(s) 296, 309, 1806, 3014
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 9 cut(s) 853, 1250, 1302, 1365, 1641, 1651, 2199, 2438, 2545
AcuI CTGAAG 2 cut(s) 768, 3141
AdeI CACNNNGTG 1 cut(s) 16
AfaI GTAC 4 cut(s) 5, 1274, 1775, 2289
AfiI CCNNNNNNNGG 6 cut(s) 219, 614, 777, 821, 1068, 2010
AhlI ACTAGT 2 cut(s) 235, 682
AjnI CCWGG 6 cut(s) 1561, 1763, 2026, 2455, 3022, 3096
AjuI GAANNNNNNNTTGG 2 cut(s) 1658, 1690
AloI GAACNNNNNNTCC 4 cut(s) 1557, 1589, 2451, 2483
Alw26I GTCTC 6 cut(s) 1514, 2332, 2404, 2618, 2988, 3185
AlwI GGATC 4 cut(s) 296, 309, 1806, 3014
AlwNI CAGNNNCTG 2 cut(s) 2341, 2591
Ama87I CYCGRG 1 cut(s) 1067
AoxI GGCC 4 cut(s) 61, 281, 1059, 2024
ApoI RAATTY 9 cut(s) 853, 1250, 1302, 1365, 1641, 1651, 2199, 2438, 2545
ArsI GACNNNNNNTTYG 2 cut(s) 3061, 3093
AseI ATTAAT 1 cut(s) 1746
Asp700I GAANNNNTTC 4 cut(s) 237, 1629, 1818, 3063
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 4 cut(s) 1060, 1844, 2821, 3249
AsuC2I CCSGG 2 cut(s) 1068, 1069
AsuHPI GGTGA 8 cut(s) 64, 1237, 2082, 2674, 2885, 3043, 3218, 3242
AvaI CYCGRG 1 cut(s) 1067
AvaII GGWCC 3 cut(s) 1844, 2821, 3249
BalI TGGCCA 1 cut(s) 283
BamHI GGATCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 2962
BbsI GAAGAC 5 cut(s) 133, 1223, 1962, 2911, 3071
BccI CCATC 8 cut(s) 390, 562, 1037, 1071, 1405, 2494, 2582, 2864
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 6 cut(s) 1563, 1765, 2028, 2457, 3024, 3098
BciVI GTATCC 3 cut(s) 254, 1904, 1984
BclI TGATCA 2 cut(s) 1495, 2307
BcnI CCSGG 2 cut(s) 1068, 1069
BcoDI GTCTC 6 cut(s) 1514, 2332, 2404, 2618, 2988, 3185
BcuI ACTAGT 2 cut(s) 235, 682
BfaI CTAG 7 cut(s) 236, 683, 734, 1083, 1118, 2811, 2973
BfmI CTRYAG 4 cut(s) 975, 1015, 2485, 2583
BfoI RGCGCY 1 cut(s) 364
BfuAI ACCTGC 2 cut(s) 851, 2908
BfuI GTATCC 3 cut(s) 254, 1904, 1984
BglI GCCNNNNNGGC 1 cut(s) 2023
BglII AGATCT 5 cut(s) 695, 999, 1019, 1336, 1996
BmcAI AGTACT 1 cut(s) 1775
Bme1390I CCNGG 8 cut(s) 1068, 1069, 1563, 1765, 2028, 2457, 3024, 3098
Bme18I GGWCC 3 cut(s) 1844, 2821, 3249
BmeT110I CYCGRG 1 cut(s) 1067
BmgT120I GGNCC 4 cut(s) 1060, 1844, 2821, 3249
BmiI GGNNCC 7 cut(s) 303, 1575, 1845, 2822, 2823, 2959, 3250
BmrFI CCNGG 8 cut(s) 1068, 1069, 1563, 1765, 2028, 2457, 3024, 3098
BmrI ACTGGG 1 cut(s) 2105
BmsI GCATC 3 cut(s) 218, 1130, 1621
BmuI ACTGGG 1 cut(s) 2105
BpiI GAAGAC 5 cut(s) 133, 1223, 1962, 2911, 3071
BplI GAGNNNNNCTC 4 cut(s) 166, 198, 391, 423
Bpu10I CCTNAGC 3 cut(s) 1113, 2205, 2850
BpuEI CTTGAG 3 cut(s) 712, 1297, 3242
BpuMI CCSGG 2 cut(s) 1068, 1069
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 4 cut(s) 553, 711, 1024, 3018
BsaI GGTCTC 2 cut(s) 2404, 3185
BsaJI CCNNGG 5 cut(s) 1067, 1900, 2176, 2815, 2978
BsaWI WCCGGW 1 cut(s) 2085
BsaXI ACNNNNNCTCC 6 cut(s) 1432, 1462, 2889, 2919, 3165, 3195
Bsc4I CCNNNNNNNGG 6 cut(s) 219, 614, 777, 821, 1068, 2010
Bse118I RCCGGY 1 cut(s) 1969
Bse1I ACTGG 5 cut(s) 84, 1595, 2100, 2110, 3172
Bse3DI GCAATG 1 cut(s) 2642
Bse8I GATNNNNATC 4 cut(s) 553, 711, 1024, 3018
BseBI CCWGG 6 cut(s) 1563, 1765, 2028, 2457, 3024, 3098
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 5 cut(s) 1067, 1900, 2176, 2815, 2978
BseGI GGATG 9 cut(s) 115, 209, 235, 554, 604, 659, 786, 1063, 1425
BseJI GATNNNNATC 4 cut(s) 553, 711, 1024, 3018
BseLI CCNNNNNNNGG 6 cut(s) 219, 614, 777, 821, 1068, 2010
BseMI GCAATG 1 cut(s) 2642
BseMII CTCAG 8 cut(s) 14, 98, 321, 624, 632, 1761, 2196, 2864
BseNI ACTGG 5 cut(s) 84, 1595, 2100, 2110, 3172
BsgI GTGCAG 1 cut(s) 2886
Bsh1236I CGCG 1 cut(s) 1979
Bsh1285I CGRYCG 1 cut(s) 2690
BshFI GGCC 4 cut(s) 63, 283, 1061, 2026
BshVI ATCGAT 1 cut(s) 711
BsiEI CGRYCG 1 cut(s) 2690
BsiHKCI CYCGRG 1 cut(s) 1067
BsiSI CCGG 4 cut(s) 1068, 1438, 1970, 2086
BslFI GGGAC 2 cut(s) 2522, 2834
BslI CCNNNNNNNGG 6 cut(s) 219, 614, 777, 821, 1068, 2010
BsmAI GTCTC 6 cut(s) 1514, 2332, 2404, 2618, 2988, 3185
BsmBI CGTCTC 1 cut(s) 2988
BsmFI GGGAC 2 cut(s) 2522, 2834
BsmI GAATGC 2 cut(s) 2851, 3157
BsnI GGCC 4 cut(s) 63, 283, 1061, 2026
Bso31I GGTCTC 2 cut(s) 2404, 3185
BsoBI CYCGRG 1 cut(s) 1067
Bsp1286I GDGCHC 1 cut(s) 2962
Bsp1407I TGTACA 1 cut(s) 3
Bsp19I CCATGG 1 cut(s) 2978
BspACI CCGC 2 cut(s) 75, 1977
BspANI GGCC 4 cut(s) 63, 283, 1061, 2026
BspCNI CTCAG 8 cut(s) 15, 97, 320, 623, 633, 1762, 2197, 2863
BspDI ATCGAT 1 cut(s) 711
BspFNI CGCG 1 cut(s) 1979
BspHI TCATGA 3 cut(s) 2863, 3082, 3147
BspLI GGNNCC 7 cut(s) 303, 1575, 1845, 2822, 2823, 2959, 3250
BspMAI CTGCAG 1 cut(s) 2587
BspMI ACCTGC 2 cut(s) 851, 2908
BspPI GGATC 4 cut(s) 296, 309, 1806, 3014
BspTNI GGTCTC 2 cut(s) 2404, 3185
BsrDI GCAATG 1 cut(s) 2642
BsrFI RCCGGY 1 cut(s) 1969
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 84, 1595, 2100, 2110, 3172
BssAI RCCGGY 1 cut(s) 1969
BssECI CCNNGG 5 cut(s) 1067, 1900, 2176, 2815, 2978
BssNAI GTATAC 1 cut(s) 1123
BssT1I CCWWGG 3 cut(s) 1900, 2815, 2978
Bst1107I GTATAC 1 cut(s) 1123
Bst2UI CCWGG 6 cut(s) 1563, 1765, 2028, 2457, 3024, 3098
Bst6I CTCTTC 5 cut(s) 1546, 2877, 2978, 3003, 3069
BstAPI GCANNNNNTGC 2 cut(s) 1095, 2591
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 816
BstDSI CCRYGG 2 cut(s) 2176, 2978
BstEII GGTNACC 1 cut(s) 2088
BstF5I GGATG 9 cut(s) 115, 209, 235, 554, 604, 659, 786, 1063, 1425
BstFNI CGCG 1 cut(s) 1979
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 1 cut(s) 363
BstMAI GTCTC 6 cut(s) 1514, 2332, 2404, 2618, 2988, 3185
BstMCI CGRYCG 1 cut(s) 2690
BstMWI GCNNNNNNNGC 7 cut(s) 1095, 1114, 1920, 2023, 2579, 2588, 2591
BstNI CCWGG 6 cut(s) 1563, 1765, 2028, 2457, 3024, 3098
BstNSI RCATGY 1 cut(s) 466
BstPI GGTNACC 1 cut(s) 2088
BstSCI CCNGG 8 cut(s) 1066, 1067, 1561, 1763, 2026, 2455, 3022, 3096
BstSFI CTRYAG 4 cut(s) 975, 1015, 2485, 2583
BstUI CGCG 1 cut(s) 1979
BstV2I GAAGAC 5 cut(s) 133, 1223, 1962, 2911, 3071
BstX2I RGATCY 6 cut(s) 301, 695, 999, 1019, 1336, 1996
BstXI CCANNNNNNTGG 1 cut(s) 2058
BstYI RGATCY 6 cut(s) 301, 695, 999, 1019, 1336, 1996
BstZ17I GTATAC 1 cut(s) 1123
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 3 cut(s) 254, 1904, 1984
BsuRI GGCC 4 cut(s) 63, 283, 1061, 2026
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 2 cut(s) 2176, 2978
BtsCI GGATG 9 cut(s) 115, 209, 235, 554, 604, 659, 786, 1063, 1425
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 5 cut(s) 77, 795, 2093, 2117, 3165
BveI ACCTGC 2 cut(s) 851, 2908
Cac8I GCNNGC 1 cut(s) 816
CaiI CAGNNNCTG 2 cut(s) 2341, 2591
CciI TCATGA 3 cut(s) 2863, 3082, 3147
CfoI GCGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 1969
Cfr13I GGNCC 4 cut(s) 1060, 1844, 2821, 3249
Cfr9I CCCGGG 1 cut(s) 1067
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 1850
CsiI ACCWGGT 1 cut(s) 1763
Csp6I GTAC 4 cut(s) 4, 1273, 1774, 2288
CviQI GTAC 4 cut(s) 4, 1273, 1774, 2288
DraIII CACNNNGTG 1 cut(s) 16
DrdI GACNNNNNNGTC 1 cut(s) 2828
DseDI GACNNNNNNGTC 1 cut(s) 2828
EaeI YGGCCR 1 cut(s) 281
Eam1104I CTCTTC 5 cut(s) 1546, 2877, 2978, 3003, 3069
EarI CTCTTC 5 cut(s) 1546, 2877, 2978, 3003, 3069
Eco130I CCWWGG 3 cut(s) 1900, 2815, 2978
Eco24I GRGCYC 1 cut(s) 2962
Eco31I GGTCTC 2 cut(s) 2404, 3185
Eco32I GATATC 2 cut(s) 709, 2662
Eco47I GGWCC 3 cut(s) 1844, 2821, 3249
Eco57I CTGAAG 2 cut(s) 768, 3141
Eco88I CYCGRG 1 cut(s) 1067
Eco91I GGTNACC 1 cut(s) 2088
EcoO109I RGGNCCY 3 cut(s) 1844, 2821, 3249
EcoO65I GGTNACC 1 cut(s) 2088
EcoRII CCWGG 6 cut(s) 1561, 1763, 2026, 2455, 3022, 3096
EcoRV GATATC 2 cut(s) 709, 2662
EcoT14I CCWWGG 3 cut(s) 1900, 2815, 2978
EcoT22I ATGCAT 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 2962
ErhI CCWWGG 3 cut(s) 1900, 2815, 2978
Esp3I CGTCTC 1 cut(s) 2988
FaqI GGGAC 2 cut(s) 2522, 2834
FbaI TGATCA 2 cut(s) 1495, 2307
FblI GTMKAC 1 cut(s) 1122
FokI GGATG 9 cut(s) 122, 196, 242, 541, 591, 666, 793, 1050, 1432
FriOI GRGCYC 1 cut(s) 2962
FspBI CTAG 7 cut(s) 236, 683, 734, 1083, 1118, 2811, 2973
GlaI GCGC 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 4 cut(s) 63, 283, 1061, 2026
HapII CCGG 4 cut(s) 1068, 1438, 1970, 2086
HgaI GACGC 1 cut(s) 1850
HhaI GCGC 1 cut(s) 363
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 3 cut(s) 688, 1258, 3196
HindII GTYRAC 3 cut(s) 688, 1258, 3196
HindIII AAGCTT 1 cut(s) 629
HpaII CCGG 4 cut(s) 1068, 1438, 1970, 2086
HphI GGTGA 8 cut(s) 64, 1237, 2082, 2674, 2885, 3043, 3218, 3242
Hpy166II GTNNAC 8 cut(s) 6, 40, 72, 688, 970, 1123, 1258, 3196
Hpy8I GTNNAC 8 cut(s) 6, 40, 72, 688, 970, 1123, 1258, 3196
HpyAV CCTTC 7 cut(s) 82, 112, 644, 2084, 2973, 3264, 3268
HpyCH4IV ACGT 1 cut(s) 1959
HpyF10VI GCNNNNNNNGC 7 cut(s) 1095, 1114, 1920, 2023, 2579, 2588, 2591
HpySE526I ACGT 1 cut(s) 1959
HspAI GCGC 1 cut(s) 361
KflI GGGWCCC 1 cut(s) 2821
Ksp22I TGATCA 2 cut(s) 1495, 2307
LmnI GCTCC 1 cut(s) 2957
LweI GCATC 3 cut(s) 218, 1130, 1621
MabI ACCWGGT 1 cut(s) 1763
MaeI CTAG 7 cut(s) 236, 683, 734, 1083, 1118, 2811, 2973
MaeII ACGT 1 cut(s) 1959
MaeIII GTNAC 8 cut(s) 529, 1448, 1753, 1759, 2088, 2124, 2345, 2680
MfeI CAATTG 3 cut(s) 819, 2329, 2655
MflI RGATCY 6 cut(s) 301, 695, 999, 1019, 1336, 1996
MhlI GDGCHC 1 cut(s) 2962
MlsI TGGCCA 1 cut(s) 283
MluNI TGGCCA 1 cut(s) 283
MlyI GAGTC 6 cut(s) 458, 652, 761, 2132, 2306, 3040
MmeI TCCRAC 4 cut(s) 426, 432, 595, 816
Mox20I TGGCCA 1 cut(s) 283
Mph1103I ATGCAT 1 cut(s) 211
MroXI GAANNNNTTC 4 cut(s) 237, 1629, 1818, 3063
MscI TGGCCA 1 cut(s) 283
MseI TTAA 9 cut(s) 392, 851, 1746, 1917, 2079, 2136, 2169, 2754, 2784
MslI CAYNNNNRTG 2 cut(s) 104, 2868
Msp20I TGGCCA 1 cut(s) 283
MspA1I CMGCKG 3 cut(s) 811, 2484, 2588
MspI CCGG 4 cut(s) 1068, 1438, 1970, 2086
MspR9I CCNGG 8 cut(s) 1068, 1069, 1563, 1765, 2028, 2457, 3024, 3098
MunI CAATTG 3 cut(s) 819, 2329, 2655
Mva1269I GAATGC 2 cut(s) 2851, 3157
MvaI CCWGG 6 cut(s) 1563, 1765, 2028, 2457, 3024, 3098
MvnI CGCG 1 cut(s) 1979
MwoI GCNNNNNNNGC 7 cut(s) 1095, 1114, 1920, 2023, 2579, 2588, 2591
NciI CCSGG 2 cut(s) 1068, 1069
NcoI CCATGG 1 cut(s) 2978
NlaIV GGNNCC 7 cut(s) 303, 1575, 1845, 2822, 2823, 2959, 3250
NmuCI GTSAC 5 cut(s) 1448, 2088, 2124, 2345, 2680
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 466
PagI TCATGA 3 cut(s) 2863, 3082, 3147
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 2 cut(s) 2851, 3157
PdmI GAANNNNTTC 4 cut(s) 237, 1629, 1818, 3063
PfoI TCCNGGA 3 cut(s) 1561, 2455, 3022
Ple19I CGATCG 1 cut(s) 2690
PleI GAGTC 6 cut(s) 457, 651, 761, 2131, 2305, 3040
PpsI GAGTC 6 cut(s) 457, 651, 761, 2131, 2305, 3040
PpuMI RGGWCCY 3 cut(s) 1844, 2821, 3249
PshBI ATTAAT 1 cut(s) 1746
Psp5II RGGWCCY 3 cut(s) 1844, 2821, 3249
Psp6I CCWGG 6 cut(s) 1561, 1763, 2026, 2455, 3022, 3096
PspEI GGTNACC 1 cut(s) 2088
PspGI CCWGG 6 cut(s) 1561, 1763, 2026, 2455, 3022, 3096
PspN4I GGNNCC 7 cut(s) 303, 1575, 1845, 2822, 2823, 2959, 3250
PspPI GGNCC 4 cut(s) 1060, 1844, 2821, 3249
PspPPI RGGWCCY 3 cut(s) 1844, 2821, 3249
PstI CTGCAG 1 cut(s) 2587
PstNI CAGNNNCTG 2 cut(s) 2341, 2591
PsuI RGATCY 6 cut(s) 301, 695, 999, 1019, 1336, 1996
PvuI CGATCG 1 cut(s) 2690
PvuII CAGCTG 3 cut(s) 811, 2484, 2588
RsaI GTAC 4 cut(s) 5, 1274, 1775, 2289
RsaNI GTAC 4 cut(s) 4, 1273, 1774, 2288
RseI CAYNNNNRTG 2 cut(s) 104, 2868
SaqAI TTAA 9 cut(s) 392, 851, 1746, 1917, 2079, 2136, 2169, 2754, 2784
Sau96I GGNCC 4 cut(s) 1060, 1844, 2821, 3249
ScaI AGTACT 1 cut(s) 1775
SchI GAGTC 6 cut(s) 458, 652, 761, 2132, 2306, 3040
ScrFI CCNGG 8 cut(s) 1068, 1069, 1563, 1765, 2028, 2457, 3024, 3098
SduI GDGCHC 1 cut(s) 2962
SexAI ACCWGGT 1 cut(s) 1763
SfaNI GCATC 3 cut(s) 218, 1130, 1621
SfcI CTRYAG 4 cut(s) 975, 1015, 2485, 2583
SinI GGWCC 3 cut(s) 1844, 2821, 3249
SmaI CCCGGG 1 cut(s) 1069
SmiMI CAYNNNNRTG 2 cut(s) 104, 2868
SmlI CTYRAG 3 cut(s) 691, 1276, 3257
SmoI CTYRAG 3 cut(s) 691, 1276, 3257
SpeI ACTAGT 2 cut(s) 235, 682
SsiI CCGC 2 cut(s) 75, 1977
SspI AATATT 3 cut(s) 964, 2383, 3089
SspMI CTAG 7 cut(s) 236, 683, 734, 1083, 1118, 2811, 2973
StyD4I CCNGG 8 cut(s) 1066, 1067, 1561, 1763, 2026, 2455, 3022, 3096
StyI CCWWGG 3 cut(s) 1900, 2815, 2978
TaiI ACGT 1 cut(s) 1962
TaqI TCGA 8 cut(s) 173, 711, 995, 1297, 1587, 1713, 1734, 3117
TatI WGTACW 3 cut(s) 3, 1272, 1773
Tru1I TTAA 9 cut(s) 392, 851, 1746, 1917, 2079, 2136, 2169, 2754, 2784
Tru9I TTAA 9 cut(s) 392, 851, 1746, 1917, 2079, 2136, 2169, 2754, 2784
TscAI CASTG 5 cut(s) 84, 802, 2100, 2117, 3172
TseFI GTSAC 5 cut(s) 1448, 2088, 2124, 2345, 2680
Tsp45I GTSAC 5 cut(s) 1448, 2088, 2124, 2345, 2680
TspGWI ACGGA 2 cut(s) 2165, 2391
TspMI CCCGGG 1 cut(s) 1067
TspRI CASTG 5 cut(s) 84, 802, 2100, 2117, 3172
VpaK11BI GGWCC 3 cut(s) 1844, 2821, 3249
VspI ATTAAT 1 cut(s) 1746
XapI RAATTY 9 cut(s) 853, 1250, 1302, 1365, 1641, 1651, 2199, 2438, 2545
XceI RCATGY 1 cut(s) 466
XcmI CCANNNNNNNNNTGG 2 cut(s) 1326, 2223
XmaI CCCGGG 1 cut(s) 1067
XmiI GTMKAC 1 cut(s) 1122
XmnI GAANNNNTTC 4 cut(s) 237, 1629, 1818, 3063
XspI CTAG 7 cut(s) 236, 683, 734, 1083, 1118, 2811, 2973
ZrmI AGTACT 1 cut(s) 1775
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.