Rroxscaffold_3G00228710

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0021530
Physical Location & Seq
Forward (+)
13125116 .. 13129967
4852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228710.1

Sequence Viewer

Length: 2916 bp
ATGACCACTCAAGGAGCCTCTTCCTCTTCCTCTGGTTTCACTCATTCATGGAAATACGATGTCTTTTTGAATTTTAGAGGCACAGATACACGCAACAATTTCACAGGTCAGTTGTACAACTACTTGACTAAAAAGGGAATCAAAACCTTTATGGATGATGAGCTTTGTAAAGGACAGGAAATATCGCAATCGCTTCTCACAGCAATTGAAGGATCCAGGATTTCTGTCGTGGTGTTCTCTCAAAATTATGCATCTTCAAAGTGGTGTCTGGATGAACTTGTGAAAATTCTTCAATGTAGAGAATCAAGGCATCAACTGGTGTTGCCAATTTTTTACAAGGTGGAACCTGCAGATGTCAGAAACCAAAGAGGTAGTTTTGATGAGCCACTTTCTCGCCATAAGTGCAAATTCAAGGATGATCTGGACAAAGTTCTAAGATGGAGAACTGCTCTTACACATGCAGCAAACTTATCTGGATGGACTTTCTCAAAAGGAGATACACATTTACTGTGTGCTCATCAAGTAAATCTGATATACGAGGTTAAGAAATTATGCTTGAAAGAAGCTTGTGAGCTCTTCAATAGTTGGAATGGTCGGTTAGCAAATAGAGAATTGGTTGATTATGAGAATTGTTCTGACCAAATTACTCGCGAATTGCAGAGTGTAGTACATTATGCTCAAGGCTTACCATTAGCTCTTAGAGTTTTAGGGTCGGTTTTACGTGGTAGAAGTATAGATGAATGGAAAGTTGTATTAGATAGTTATAAAAAAGTTCCTAACAAAGAGATTCAAGATATTCTCAAAATCAGTTTTGAGGCACTAGAAGATCCGGTCAAAGAAGTTTTCCTCGACCTTGCATGTTTCTTGAAAGGTAGAAACAAAGACTATGTAATGCAAGCACTGGACGGTTCTGACCTTAACCCCGAGTTTTCCATTACAGTTCTAGTAGAAAAGGCGCTCATAAACATTGATGAGAACAAGTGTATTTGGATGCATGACTTGATGGAAGAAATGGGTAAAGAAATCATTCGTCAAGAGTCACCTGGAGAACCTGGTAAACGTAGCAGATTATGGTTTCATGAAGATGTCTATGATGTTCTGACAGAAAATACTGGAACAAACAAAGTTAAAGGCATCATTGTGAAATTGCCTCAGCCAATTGAGATATCCTTGAATGCAAAATGCTTTAAAAAGATGAAAAGTCTTCAACTTTTTATAAACATTAATGCAAGCTTCTCAGGGGAGGTTCGTTATCTCCCTAACCAATTGAGGTTCCTTGATTGGCCTGGATGTCCACTACAATCTTTGCCATCCAACTTCAATCCGAAGAATCTTGTTTCATTCAATATGCCTGGAAGTCAAATCTCACGGTTGGGTGGAGGATTCAAGAGTTTCCACCGTTTGAAATCTATGAACTTGGAAGGTTGTAACTCCTTGACAGAAGTCCCCAACTTCTCTGGCATGTTCCCAAGCTTAGAGACCCTATGTCTAAGCTATTGTATGAGTTTGGTTGAGCTGCATCCTTCTGTTGGATCCCTTGAAAAACTTGTTGACATAAATCTTGCAGGATGCTATAGCCTCACTATGTTTCCAAGAATAGACAACTCAACTTCTTTGCGTTCTATTGACCTTAGAGGCTGCACTAGGCTTAAGCATTTTCCGGAAATCGAGGAGAAGGTGGAATCCTTAACAACATTGATTCTCACAAAGAGTGGCATCAAAGAGTTGCCTTCATCAATTAACAATCTCATTAGTCTCGAAGTGTTACATGTCGGATTTTGTAGCAGCCTCGCGCAGATTCCACAGAGCATTTATGACTTGCAGCAGTTACAGACGTTCTGTTTACAAAATGGTCCAAAGTTTGTTACCCTTACTAATACAATGAACTCTGGAGTGCTTCCGACTAATCCAAATGTTGCAGATGACAAATATGGCTCTACACCAGAAAGTAATGATGAAACCAGTTTACGGCTTCCAAATCTACGAAGTTTCATAGCAGGAGACTGCAATTTATCGGACACTGATTTCCTTGTTAGTCTCGATTGTGTGTCAACACTAGAAAGATTAGATCTCTCCGGAAATATTTTTGAAAGTCTTCCTCTATGCTTTACCAAATTTGCCAGGTTGACATATATTTACTTGGATGGTTGCAAAAGGCTGCGAGAAATTCCAGAACTTCCACCAAACGTGGAAAGTTTACATGCGAGAAAGTGTGTTTCATTGAAAAGAATTGCAAATCTGTCAAACATTTTGGAACAAAAAGGGTCAAAAATGATTAAACATATGAATTTGACAAATTGTCGGAGACTGCGTGATCGTCTGGCTCGGGACGTCAAGAAGATGAATAGTACTTTACTGATCAATGAGGTCACTCTCTTCTCCCTCTGTCTCTCTTCTCTACAATCTGAGTTCCAGGTTGTATTTCCAGCAAGTGATGTTCCAGAGTGGTTTAGCTGTCAAATGGATTTCACTGCAGGGTTTCCCTTTTATGAATTTTCTTTTGACATCGTTCCACATTTGCAATTAGAGAACACAGGACTGGCCATCTGTGTTGCTCTTGACAAGCCACAATCTGGTTACCGTTATCAGTGCGACTTCGAGGTCCATTTTTCTTTCAATCATCATCGTCCTTGGCCCGATAGGGTCCTCGGAGAAATTGATGCAACAGAGCCGGCTCATGTGTTCATGTATTATTACCCCTTCTGTAAAATTCCGCCATTTCTTCATTTAATTCCTCCGCCTGTTACATTTCGAGTTACAATCGTATGTCGTCTCGCCGACGATGGGGACGACCCTCCTATCAAAAGCTGCGGGGTACATCTTGTAATGCCACCAAATGAGGACGTTTCCATAAAGATTTCTAAGTCACAGAAGCTCCCGCCCATAGAGACTCTTGACCTGAAGACTAGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

971

Amino Acids

110.16

Weight (kDa)

6.54

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 167 3.6e-50 TIR domain
TIR_2 PF13676 21 - 117 1.1e-14 TIR domain
WHD_ROQ1 PF23282 279 - 347 9.2e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 531 - 615 2.1e-08 Leucine-rich repeat region
LRR_13 PF23286 538 - 614 3.6e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 659 - 752 4.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021530)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014343
rosa_multiflora Rmu_sc0000565.1_g000040
rosa_roxburghii Rroxscaffold_3G00228710 Rroxscaffold_7G00204260 Rroxscaffold_7G00204440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 765, 1217
AasI GACNNNNNNGTC 1 cut(s) 2600
AatII GACGTC 1 cut(s) 2334
Acc36I ACCTGC 1 cut(s) 355
AccB7I CCANNNNNTGG 1 cut(s) 2573
AccII CGCG 2 cut(s) 651, 1793
AccIII TCCGGA 2 cut(s) 1660, 2075
AciI CCGC 4 cut(s) 2714, 2738, 2811, 2879
AclWI GGATC 5 cut(s) 207, 220, 821, 1527, 1540
AcoI YGGCCR 1 cut(s) 2541
AcsI RAATTY 8 cut(s) 70, 285, 407, 2114, 2166, 2287, 2492, 2709
AcyI GRCGYC 1 cut(s) 2331
AfaI GTAC 4 cut(s) 116, 669, 2350, 2817
AfiI CCNNNNNNNGG 4 cut(s) 1529, 1968, 2573, 2784
AflII CTTAAG 1 cut(s) 1649
AflIII ACRYGT 1 cut(s) 1768
AhdI GACNNNNNGTC 2 cut(s) 1485, 2298
AjnI CCWGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
Alw21I GWGCWC 2 cut(s) 517, 576
Alw26I GTCTC 8 cut(s) 1472, 1760, 1995, 2042, 2299, 2393, 2777, 2882
AlwI GGATC 5 cut(s) 207, 220, 821, 1527, 1540
Ama87I CYCGRG 2 cut(s) 923, 2325
Aor13HI TCCGGA 2 cut(s) 1660, 2075
AoxI GGCC 4 cut(s) 1283, 2541, 2633, 2910
ApeKI GCWGC 7 cut(s) 461, 1516, 1638, 1785, 1822, 2158, 2808
ApoI RAATTY 8 cut(s) 70, 285, 407, 2114, 2166, 2287, 2492, 2709
AseI ATTAAT 1 cut(s) 1224
Asp700I GAANNNNTTC 2 cut(s) 1026, 2094
AspLEI GCGC 2 cut(s) 958, 1795
AspS9I GGNCC 5 cut(s) 1853, 2602, 2634, 2644, 2910
AsuHPI GGTGA 1 cut(s) 1032
AvaI CYCGRG 2 cut(s) 923, 2325
AvaII GGWCC 3 cut(s) 1853, 2602, 2644
BalI TGGCCA 1 cut(s) 2543
BamHI GGATCC 2 cut(s) 212, 1532
BanII GRGCYC 1 cut(s) 576
BbsI GAAGAC 3 cut(s) 1196, 2087, 2909
Bbv12I GWGCWC 2 cut(s) 517, 576
BbvCI CCTCAGC 1 cut(s) 1152
BbvI GCAGC 7 cut(s) 473, 1503, 1625, 1797, 1834, 2145, 2795
BccI CCATC 7 cut(s) 432, 471, 997, 1318, 2138, 2552, 2777
BceAI ACGGC 1 cut(s) 1985
BciT130I CCWGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
BclI TGATCA 1 cut(s) 2358
BcoDI GTCTC 8 cut(s) 1472, 1760, 1995, 2042, 2299, 2393, 2777, 2882
BfaI CTAG 5 cut(s) 821, 944, 1644, 2057, 2907
BfmI CTRYAG 3 cut(s) 348, 1573, 2472
BfoI RGCGCY 1 cut(s) 959
BfrI CTTAAG 1 cut(s) 1649
BfuAI ACCTGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 2068
BisI GCNGC 7 cut(s) 462, 1517, 1639, 1786, 1823, 2159, 2809
BlsI GCNGC 7 cut(s) 463, 1518, 1640, 1787, 1824, 2160, 2810
BmcAI AGTACT 1 cut(s) 2350
Bme1390I CCNGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
Bme18I GGWCC 3 cut(s) 1853, 2602, 2644
BmeRI GACNNNNNGTC 2 cut(s) 1485, 2298
BmeT110I CYCGRG 2 cut(s) 923, 2325
BmgT120I GGNCC 5 cut(s) 1853, 2602, 2634, 2644, 2910
BmiI GGNNCC 6 cut(s) 16, 214, 345, 1274, 1534, 2645
BmrFI CCNGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
BmsI GCATC 8 cut(s) 260, 319, 981, 1143, 1528, 1559, 1725, 2650
BoxI GACNNNNGTC 1 cut(s) 1442
BpiI GAAGAC 3 cut(s) 1196, 2087, 2909
BplI GAGNNNNNCTC 2 cut(s) 433, 465
BpmI CTGGAG 2 cut(s) 1065, 1911
Bpu10I CCTNAGC 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 2331
BsaI GGTCTC 1 cut(s) 1472
BsaJI CCNNGG 2 cut(s) 2630, 2647
BsaWI WCCGGW 3 cut(s) 829, 1660, 2075
BsaXI ACNNNNNCTCC 4 cut(s) 2296, 2326, 2859, 2889
Bsc4I CCNNNNNNNGG 4 cut(s) 1529, 1968, 2573, 2784
Bse118I RCCGGY 1 cut(s) 2671
Bse1I ACTGG 5 cut(s) 321, 906, 1117, 1962, 2544
BseAI TCCGGA 2 cut(s) 1660, 2075
BseBI CCWGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
BseDI CCNNGG 2 cut(s) 2630, 2647
BseLI CCNNNNNNNGG 4 cut(s) 1529, 1968, 2573, 2784
BseMII CTCAG 3 cut(s) 1166, 1251, 2397
BseNI ACTGG 5 cut(s) 321, 906, 1117, 1962, 2544
BseRI GAGGAG 1 cut(s) 1685
BseXI GCAGC 7 cut(s) 473, 1503, 1625, 1797, 1834, 2145, 2795
BsgI GTGCAG 1 cut(s) 1624
Bsh1236I CGCG 2 cut(s) 651, 1793
BshFI GGCC 4 cut(s) 1285, 2543, 2635, 2912
BsiHKAI GWGCWC 2 cut(s) 517, 576
BsiHKCI CYCGRG 2 cut(s) 923, 2325
BsiSI CCGG 4 cut(s) 830, 1661, 2076, 2672
BslFI GGGAC 3 cut(s) 1430, 2342, 2801
BslI CCNNNNNNNGG 4 cut(s) 1529, 1968, 2573, 2784
BsmAI GTCTC 8 cut(s) 1472, 1760, 1995, 2042, 2299, 2393, 2777, 2882
BsmBI CGTCTC 1 cut(s) 2777
BsmFI GGGAC 3 cut(s) 1430, 2342, 2801
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 4 cut(s) 1285, 2543, 2635, 2912
Bso31I GGTCTC 1 cut(s) 1472
BsoBI CYCGRG 2 cut(s) 923, 2325
Bsp1286I GDGCHC 2 cut(s) 517, 576
Bsp13I TCCGGA 2 cut(s) 1660, 2075
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 4 cut(s) 2714, 2738, 2811, 2879
BspANI GGCC 4 cut(s) 1285, 2543, 2635, 2912
BspCNI CTCAG 3 cut(s) 1165, 1250, 2398
BspEI TCCGGA 2 cut(s) 1660, 2075
BspFNI CGCG 2 cut(s) 651, 1793
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 6 cut(s) 16, 214, 345, 1274, 1534, 2645
BspMAI CTGCAG 2 cut(s) 352, 2476
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 5 cut(s) 207, 220, 821, 1527, 1540
BspQI GCTCTTC 1 cut(s) 581
BspTI CTTAAG 1 cut(s) 1649
BspTNI GGTCTC 1 cut(s) 1472
BsrFI RCCGGY 1 cut(s) 2671
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 5 cut(s) 321, 906, 1117, 1962, 2544
BssAI RCCGGY 1 cut(s) 2671
BssECI CCNNGG 2 cut(s) 2630, 2647
BssMI GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
BssNI GRCGYC 1 cut(s) 2331
BssT1I CCWWGG 1 cut(s) 2630
Bst2UI CCWGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
Bst4CI ACNGT 6 cut(s) 510, 908, 940, 1371, 1400, 2582
Bst6I CTCTTC 5 cut(s) 25, 31, 581, 2381, 2398
BstACI GRCGYC 1 cut(s) 2331
BstAFI CTTAAG 1 cut(s) 1649
BstAUI TGTACA 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 897, 1231, 2673
BstDEI CTNAG 9 cut(s) 434, 698, 1152, 1237, 1474, 1490, 1631, 2406, 2862
BstEII GGTNACC 1 cut(s) 2576
BstFNI CGCG 2 cut(s) 651, 1793
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 2 cut(s) 958, 1795
BstKTI GATC 7 cut(s) 215, 421, 829, 1535, 2071, 2317, 2361
BstMAI GTCTC 8 cut(s) 1472, 1760, 1995, 2042, 2299, 2393, 2777, 2882
BstMBI GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
BstNSI RCATGY 5 cut(s) 461, 861, 1465, 1772, 2204
BstPAI GACNNNNGTC 1 cut(s) 1442
BstPI GGTNACC 1 cut(s) 2576
BstSCI CCNGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
BstSFI CTRYAG 3 cut(s) 348, 1573, 2472
BstUI CGCG 2 cut(s) 651, 1793
BstV1I GCAGC 7 cut(s) 473, 1503, 1625, 1797, 1834, 2145, 2795
BstV2I GAAGAC 3 cut(s) 1196, 2087, 2909
BstX2I RGATCY 4 cut(s) 212, 826, 1532, 2068
BstYI RGATCY 4 cut(s) 212, 826, 1532, 2068
BsuRI GGCC 4 cut(s) 1285, 2543, 2635, 2912
BtsI GCAGTG 1 cut(s) 2469
BtsIMutI CAGTG 4 cut(s) 899, 2019, 2469, 2594
BtuMI TCGCGA 1 cut(s) 651
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 3 cut(s) 897, 1231, 2673
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 2 cut(s) 958, 1795
Cfr10I RCCGGY 1 cut(s) 2671
Cfr13I GGNCC 5 cut(s) 1853, 2602, 2634, 2644, 2910
CsiI ACCWGGT 1 cut(s) 1051
Csp6I GTAC 4 cut(s) 115, 668, 2349, 2816
CviQI GTAC 4 cut(s) 115, 668, 2349, 2816
DdeI CTNAG 9 cut(s) 434, 698, 1152, 1237, 1474, 1490, 1631, 2406, 2862
DpnI GATC 7 cut(s) 214, 420, 828, 1534, 2070, 2316, 2360
DpnII GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
DraI TTTAAA 1 cut(s) 1189
DrdI GACNNNNNNGTC 1 cut(s) 2600
DriI GACNNNNNGTC 2 cut(s) 1485, 2298
DseDI GACNNNNNNGTC 1 cut(s) 2600
EaeI YGGCCR 1 cut(s) 2541
Eam1104I CTCTTC 5 cut(s) 25, 31, 581, 2381, 2398
Eam1105I GACNNNNNGTC 2 cut(s) 1485, 2298
EarI CTCTTC 5 cut(s) 25, 31, 581, 2381, 2398
EciI GGCGGA 2 cut(s) 2703, 2727
Ecl136II GAGCTC 1 cut(s) 574
Eco130I CCWWGG 1 cut(s) 2630
Eco24I GRGCYC 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 1472
Eco32I GATATC 1 cut(s) 1167
Eco47I GGWCC 3 cut(s) 1853, 2602, 2644
Eco53kI GAGCTC 1 cut(s) 574
Eco88I CYCGRG 2 cut(s) 923, 2325
Eco91I GGTNACC 1 cut(s) 2576
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 2 cut(s) 2644, 2910
EcoO65I GGTNACC 1 cut(s) 2576
EcoRII CCWGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
EcoRV GATATC 1 cut(s) 1167
EcoT14I CCWWGG 1 cut(s) 2630
EcoT22I ATGCAT 2 cut(s) 253, 996
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 2630
Esp3I CGTCTC 1 cut(s) 2777
FaqI GGGAC 3 cut(s) 1430, 2342, 2801
FauI CCCGC 2 cut(s) 2804, 2886
FauNDI CATATG 1 cut(s) 2283
FbaI TGATCA 1 cut(s) 2358
Fnu4HI GCNGC 7 cut(s) 462, 1517, 1639, 1786, 1823, 2159, 2809
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 7 cut(s) 462, 1517, 1639, 1786, 1823, 2159, 2809
FspBI CTAG 5 cut(s) 821, 944, 1644, 2057, 2907
GlaI GCGC 2 cut(s) 957, 1794
GluI GCNGC 7 cut(s) 462, 1517, 1639, 1786, 1823, 2159, 2809
GsuI CTGGAG 2 cut(s) 1065, 1911
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 4 cut(s) 1285, 2543, 2635, 2912
HapII CCGG 4 cut(s) 830, 1661, 2076, 2672
HhaI GCGC 2 cut(s) 958, 1795
Hin1I GRCGYC 1 cut(s) 2331
Hin6I GCGC 2 cut(s) 956, 1793
HinP1I GCGC 2 cut(s) 956, 1793
HincII GTYRAC 3 cut(s) 1552, 2052, 2127
HindII GTYRAC 3 cut(s) 1552, 2052, 2127
HindIII AAGCTT 3 cut(s) 564, 1231, 1471
HpaII CCGG 4 cut(s) 830, 1661, 2076, 2672
HphI GGTGA 1 cut(s) 1032
Hpy166II GTNNAC 8 cut(s) 1058, 1295, 1552, 1844, 1967, 2052, 2127, 2198
Hpy8I GTNNAC 8 cut(s) 1058, 1295, 1552, 1844, 1967, 2052, 2127, 2198
Hpy99I CGWCG 1 cut(s) 2783
HpyAV CCTTC 6 cut(s) 203, 1415, 1533, 1669, 1740, 2710
HpyCH4III ACNGT 6 cut(s) 510, 908, 940, 1371, 1400, 2582
HpyCH4IV ACGT 6 cut(s) 721, 1060, 1835, 2187, 2331, 2844
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 9 cut(s) 434, 698, 1152, 1237, 1474, 1490, 1631, 2406, 2862
HpySE526I ACGT 6 cut(s) 721, 1060, 1835, 2187, 2331, 2844
Hsp92I GRCGYC 1 cut(s) 2331
HspAI GCGC 2 cut(s) 956, 1793
Kpn2I TCCGGA 2 cut(s) 1660, 2075
KroI GCCGGC 1 cut(s) 2671
KroNI GCCGGC 1 cut(s) 2673
Ksp22I TGATCA 1 cut(s) 2358
Kzo9I GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
LguI GCTCTTC 1 cut(s) 581
LmnI GCTCC 2 cut(s) 14, 2880
Lsp1109I GCAGC 7 cut(s) 473, 1503, 1625, 1797, 1834, 2145, 2795
LweI GCATC 8 cut(s) 260, 319, 981, 1143, 1528, 1559, 1725, 2650
MabI ACCWGGT 1 cut(s) 1051
MaeI CTAG 5 cut(s) 821, 944, 1644, 2057, 2907
MaeII ACGT 6 cut(s) 721, 1060, 1835, 2187, 2331, 2844
MalI GATC 7 cut(s) 214, 420, 828, 1534, 2070, 2316, 2360
MboI GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
MfeI CAATTG 3 cut(s) 204, 1158, 1265
MflI RGATCY 4 cut(s) 212, 826, 1532, 2068
MhlI GDGCHC 2 cut(s) 517, 576
MlsI TGGCCA 1 cut(s) 2543
MluNI TGGCCA 1 cut(s) 2543
MlyI GAGTC 2 cut(s) 1046, 2884
MmeI TCCRAC 6 cut(s) 566, 1338, 1510, 1753, 1925, 2282
Mox20I TGGCCA 1 cut(s) 2543
Mph1103I ATGCAT 2 cut(s) 253, 996
MroI TCCGGA 2 cut(s) 1660, 2075
MroNI GCCGGC 1 cut(s) 2671
MroXI GAANNNNTTC 2 cut(s) 1026, 2094
MscI TGGCCA 1 cut(s) 2543
MslI CAYNNNNRTG 2 cut(s) 1083, 1139
Msp20I TGGCCA 1 cut(s) 2543
MspCI CTTAAG 1 cut(s) 1649
MspI CCGG 4 cut(s) 830, 1661, 2076, 2672
MspR9I CCNGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
MunI CAATTG 3 cut(s) 204, 1158, 1265
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
MvnI CGCG 2 cut(s) 651, 1793
MwoI GCNNNNNNNGC 1 cut(s) 402
NaeI GCCGGC 1 cut(s) 2673
NdeI CATATG 1 cut(s) 2283
NdeII GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
NgoMIV GCCGGC 1 cut(s) 2671
NlaIV GGNNCC 6 cut(s) 16, 214, 345, 1274, 1534, 2645
NmuCI GTSAC 3 cut(s) 1038, 2368, 2865
NruI TCGCGA 1 cut(s) 651
NsiI ATGCAT 2 cut(s) 253, 996
NspI RCATGY 5 cut(s) 461, 861, 1465, 1772, 2204
PagI TCATGA 1 cut(s) 1078
PciI ACATGT 1 cut(s) 1768
PciSI GCTCTTC 1 cut(s) 581
PcsI WCGNNNNNNNCGW 2 cut(s) 2308, 2787
PctI GAATGC 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 2673
PdmI GAANNNNTTC 2 cut(s) 1026, 2094
PfeI GAWTC 8 cut(s) 138, 302, 787, 1330, 1383, 1682, 1699, 1798
PflMI CCANNNNNTGG 1 cut(s) 2573
PfoI TCCNGGA 1 cut(s) 215
PkrI GCNGC 7 cut(s) 463, 1518, 1640, 1787, 1824, 2160, 2810
PleI GAGTC 2 cut(s) 1045, 2884
PpsI GAGTC 2 cut(s) 1045, 2884
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 2644
PscI ACATGT 1 cut(s) 1768
PshAI GACNNNNGTC 1 cut(s) 1442
PshBI ATTAAT 1 cut(s) 1224
PsiI TTATAA 2 cut(s) 765, 1217
Psp124BI GAGCTC 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 2644
Psp6I CCWGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
PspEI GGTNACC 1 cut(s) 2576
PspGI CCWGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
PspN4I GGNNCC 6 cut(s) 16, 214, 345, 1274, 1534, 2645
PspPI GGNCC 5 cut(s) 1853, 2602, 2634, 2644, 2910
PspPPI RGGWCCY 1 cut(s) 2644
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PstI CTGCAG 2 cut(s) 352, 2476
PsuI RGATCY 4 cut(s) 212, 826, 1532, 2068
RruI TCGCGA 1 cut(s) 651
RsaI GTAC 4 cut(s) 116, 669, 2350, 2817
RsaNI GTAC 4 cut(s) 115, 668, 2349, 2816
RseI CAYNNNNRTG 2 cut(s) 1083, 1139
SacI GAGCTC 1 cut(s) 576
SapI GCTCTTC 1 cut(s) 581
SatI GCNGC 7 cut(s) 462, 1517, 1639, 1786, 1823, 2159, 2809
Sau3AI GATC 7 cut(s) 212, 418, 826, 1532, 2068, 2314, 2358
Sau96I GGNCC 5 cut(s) 1853, 2602, 2634, 2644, 2910
ScaI AGTACT 1 cut(s) 2350
SchI GAGTC 2 cut(s) 1046, 2884
ScrFI CCNGG 7 cut(s) 217, 1044, 1053, 1287, 1353, 2122, 2414
SduI GDGCHC 2 cut(s) 517, 576
SexAI ACCWGGT 1 cut(s) 1051
SfaNI GCATC 8 cut(s) 260, 319, 981, 1143, 1528, 1559, 1725, 2650
SfcI CTRYAG 3 cut(s) 348, 1573, 2472
SinI GGWCC 3 cut(s) 1853, 2602, 2644
SmiMI CAYNNNNRTG 2 cut(s) 1083, 1139
SmlI CTYRAG 3 cut(s) 9, 678, 1649
SmoI CTYRAG 3 cut(s) 9, 678, 1649
SsiI CCGC 4 cut(s) 2714, 2738, 2811, 2879
SspI AATATT 1 cut(s) 2083
SspMI CTAG 5 cut(s) 821, 944, 1644, 2057, 2907
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 7 cut(s) 215, 1042, 1051, 1285, 1351, 2120, 2412
StyI CCWWGG 1 cut(s) 2630
TaaI ACNGT 6 cut(s) 510, 908, 940, 1371, 1400, 2582
TaiI ACGT 6 cut(s) 724, 1063, 1838, 2190, 2334, 2847
TaqI TCGA 6 cut(s) 849, 1668, 1758, 2040, 2598, 2752
TatI WGTACW 3 cut(s) 114, 667, 2348
TfiI GAWTC 8 cut(s) 138, 302, 787, 1330, 1383, 1682, 1699, 1798
TscAI CASTG 4 cut(s) 906, 2026, 2476, 2594
TseFI GTSAC 3 cut(s) 1038, 2368, 2865
TseI GCWGC 7 cut(s) 461, 1516, 1638, 1785, 1822, 2158, 2808
Tsp45I GTSAC 3 cut(s) 1038, 2368, 2865
TspRI CASTG 4 cut(s) 906, 2026, 2476, 2594
Van91I CCANNNNNTGG 1 cut(s) 2573
Vha464I CTTAAG 1 cut(s) 1649
VpaK11BI GGWCC 3 cut(s) 1853, 2602, 2644
VspI ATTAAT 1 cut(s) 1224
XapI RAATTY 8 cut(s) 70, 285, 407, 2114, 2166, 2287, 2492, 2709
XceI RCATGY 5 cut(s) 461, 861, 1465, 1772, 2204
XmnI GAANNNNTTC 2 cut(s) 1026, 2094
XspI CTAG 5 cut(s) 821, 944, 1644, 2057, 2907
ZraI GACGTC 1 cut(s) 2332
ZrmI AGTACT 1 cut(s) 2350
Zsp2I ATGCAT 2 cut(s) 253, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.