RLG00000014837

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019325
Physical Location & Seq
Reverse (-)
58219333 .. 58220590
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014837

Sequence Viewer

Length: 870 bp
ATGGCCACGTATTGGAGACACTTTAGAGTTTCTAGAGATGTTGCAAAATATCCTGATGATGCTTTTAGCTTTGAAATCCATCATGGTGGATATAGGCAGCATTTGGATAATGATAGAAGGAGGTACAAGGGTGGGAGCGTGCACTTTTTGGATGGAGTCGATCCTGATCAATGTTGTTGGGTGGAATGTAACAATATAGCTTGGGATCTGGGTTATAGAGTGAGGCCAATTTCTTATTGGTACAAGCTGCCTAGAGGTGCAAAAACTGAGGGTTTTCATAGAATAGATAACGATACAAATGCTATAGAGATGACTAAGCACATACCACCAAGAAAGAGGGTAATGCAGCTATTCATAACTAGGGGTGGTCCAAGAAAGATCAAAGAGGCTGAGCTAGAGGATGAAAAACCAAAACCACAAGATTGGCACAATCCTCTGAACCTATTATTGCCATCAGAACTTGCTAGAATGAATAAAGAGGAAGTGAATAATGGTGTGGAAAGGAATGATGTTATGGCTGATTTTGAAGTGTCTAAGAATGATGAGTTGGCTATTGTCGAAGTGTCACTGAATGGTTTTGTGGGAGATACTAATGAGGTGGTAGAGAATGACTATGTTATAGAGAATGATGATGTCGAAGGTTATGCGGCAGATGATGACTTATTAATGGAAAAAAGTGCAAAAGTTAAGAAGGCTGCAAAAGGCAAAGCTAAAGTTGTTGATGGAGACAAATCTAAAAAAGCTAAGGGAAGAAAGAGTGCAAAAGCAAAGCAATACAACACTCGATTCAAGGGAGAGAAATCATTTGAAGAAGTTGACTTGGAAGATCAGACAACTGATGAGGAAGACAAAGAATTCTTTATAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

290

Amino Acids

33.44

Weight (kDa)

5.54

Isoelectric Point (pI)

27.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 19 - 119 5.3e-16 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019325)

Species Orthologous Gene IDs
pyrus_communis pycom12g06750
rosa_laevigata RLG00000014837
rosa_roxburghii Rroxscaffold_2G00117710
rosa_samantha Rh3DG349100 Rh5AG331100 Rh5BG273900 Rh5DG281800 Rh6CG333500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 12
AciI CCGC 1 cut(s) 647
AclWI GGATC 2 cut(s) 155, 213
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 854
AfaI GTAC 2 cut(s) 125, 242
AfiI CCNNNNNNNGG 1 cut(s) 12
AgsI TTSAA 4 cut(s) 74, 527, 790, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 7 cut(s) 69, 200, 247, 349, 394, 710, 743
AluI AGCT 7 cut(s) 69, 200, 247, 349, 394, 710, 743
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 10, 720
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 2 cut(s) 155, 213
AoxI GGCC 2 cut(s) 3, 224
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 4 cut(s) 97, 247, 346, 695
ApoI RAATTY 1 cut(s) 854
AseI ATTAAT 1 cut(s) 665
AspS9I GGNCC 1 cut(s) 368
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 144
BaeI ACNNNNGTAYC 2 cut(s) 115, 148
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 109, 234, 358, 682
BccI CCATC 4 cut(s) 87, 146, 460, 716
BcgI CGANNNNNNTGC 4 cut(s) 281, 315, 626, 660
BclI TGATCA 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 10, 720
BfaI CTAG 5 cut(s) 33, 252, 360, 395, 465
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 5 cut(s) 98, 248, 347, 648, 696
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 5 cut(s) 99, 249, 348, 649, 697
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 852
Bpu10I CCTNAGC 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 390
BsaAI YACGTR 1 cut(s) 9
BsaBI GATNNNNATC 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 165
BseGI GGATG 2 cut(s) 157, 406
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 258, 381
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 4 cut(s) 109, 234, 358, 682
BshFI GGCC 2 cut(s) 5, 226
BsiHKAI GWGCWC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 10, 720
BsnI GGCC 2 cut(s) 5, 226
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 5 cut(s) 160, 166, 205, 378, 826
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 1 cut(s) 647
BspANI GGCC 2 cut(s) 5, 226
BspCNI CTCAG 2 cut(s) 259, 382
BspPI GGATC 2 cut(s) 155, 213
BssMI GATC 5 cut(s) 160, 166, 205, 378, 826
BstBAI YACGTR 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 5 cut(s) 267, 315, 390, 534, 744
BstF5I GGATG 2 cut(s) 157, 406
BstKTI GATC 5 cut(s) 163, 169, 208, 381, 829
BstMAI GTCTC 2 cut(s) 10, 720
BstMBI GATC 5 cut(s) 160, 166, 205, 378, 826
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 109, 234, 358, 682
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 205
BstXI CCANNNNNNTGG 2 cut(s) 86, 423
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 2 cut(s) 5, 226
BtsCI GGATG 2 cut(s) 157, 406
BtsIMutI CAGTG 1 cut(s) 566
Cac8I GCNNGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 124, 241
CviAII CATG 1 cut(s) 83
CviQI GTAC 2 cut(s) 124, 241
DdeI CTNAG 5 cut(s) 267, 315, 390, 534, 744
DpnI GATC 5 cut(s) 162, 168, 207, 380, 828
DpnII GATC 5 cut(s) 160, 166, 205, 378, 826
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 854
FaeI CATG 1 cut(s) 86
FatI CATG 1 cut(s) 82
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 5 cut(s) 98, 248, 347, 648, 696
FokI GGATG 2 cut(s) 164, 413
Fsp4HI GCNGC 5 cut(s) 98, 248, 347, 648, 696
FspBI CTAG 5 cut(s) 33, 252, 360, 395, 465
GluI GCNGC 5 cut(s) 98, 248, 347, 648, 696
HaeIII GGCC 2 cut(s) 5, 226
Hin1II CATG 1 cut(s) 86
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 2 cut(s) 156, 786
Hpy166II GTNNAC 2 cut(s) 142, 817
Hpy188I TCNGA 3 cut(s) 438, 457, 831
Hpy188III TCNNGA 3 cut(s) 33, 53, 164
Hpy8I GTNNAC 2 cut(s) 142, 817
HpyAV CCTTC 3 cut(s) 111, 632, 685
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 7 cut(s) 44, 142, 260, 346, 680, 698, 761
HpyF3I CTNAG 5 cut(s) 267, 315, 390, 534, 744
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 5 cut(s) 160, 166, 205, 378, 826
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 3 cut(s) 66, 177, 194
Lsp1109I GCAGC 4 cut(s) 109, 234, 358, 682
LweI GCATC 1 cut(s) 49
MaeI CTAG 5 cut(s) 33, 252, 360, 395, 465
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 188, 564
MalI GATC 5 cut(s) 162, 168, 207, 380, 828
MboI GATC 5 cut(s) 160, 166, 205, 378, 826
MboII GAAGA 4 cut(s) 762, 821, 836, 857
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 144
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 228, 854
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 165
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 665, 687
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 5
NdeII GATC 5 cut(s) 160, 166, 205, 378, 826
NlaIII CATG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 564
PfeI GAWTC 1 cut(s) 786
PflMI CCANNNNNTGG 1 cut(s) 12
PkrI GCNGC 5 cut(s) 99, 249, 348, 649, 697
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Ppu21I YACGTR 1 cut(s) 9
PshBI ATTAAT 1 cut(s) 665
PspPI GGNCC 1 cut(s) 368
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 2 cut(s) 125, 242
RsaNI GTAC 2 cut(s) 124, 241
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 2 cut(s) 665, 687
SatI GCNGC 5 cut(s) 98, 248, 347, 648, 696
Sau3AI GATC 5 cut(s) 160, 166, 205, 378, 826
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 1 cut(s) 165
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 2 cut(s) 228, 854
SsiI CCGC 1 cut(s) 647
SspMI CTAG 5 cut(s) 33, 252, 360, 395, 465
TaiI ACGT 1 cut(s) 11
TaqI TCGA 4 cut(s) 159, 558, 636, 784
TasI AATT 2 cut(s) 228, 854
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 1 cut(s) 786
Tru1I TTAA 2 cut(s) 665, 687
Tru9I TTAA 2 cut(s) 665, 687
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 1 cut(s) 564
TseI GCWGC 4 cut(s) 97, 247, 346, 695
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 4 cut(s) 266, 343, 417, 485
TspRI CASTG 1 cut(s) 573
Van91I CCANNNNNTGG 1 cut(s) 12
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 368
VspI ATTAAT 1 cut(s) 665
XapI RAATTY 1 cut(s) 854
XbaI TCTAGA 1 cut(s) 32
XcmI CCANNNNNNNNNTGG 1 cut(s) 234
XspI CTAG 5 cut(s) 33, 252, 360, 395, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.