Rh5BG273900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019325
Physical Location & Seq
Forward (+)
34488530 .. 34489811
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG273900.1

Sequence Viewer

Length: 438 bp
ATGTTGCAAAATATCTTAAGAATAAGAGAACCTGGAGAAGTAGAACCACCTGCACAATCAGAAACTATACTTAAGAGGACCTTCTTTATTAAAGTTAAATGTGGATTCTGTAAAATTAAAGGCCACAATGTCAGAGGATGTCCAAAGAAAAAGGCTGTGGATGCATCAAGTTCCCAACCTGATGGTTCATGTGCTCAAGGAGCATCTCAAGGTTCAGGTGCTAAAAGTACTGCGACAACACCAAAAACTGGTCCAAATAAATCATCAAGAAAGTCGACTCAATCTCAAAAGCCAGTTGTCCCAAAGACAATAGGAATCAAAAGGCCTCAATCTCAACCTCAAACACATATGCAGGCAAAGGTGAGCGGAGCTGCAAAGAAACCAAGACAGACCAATGTCTCTTCCTCGCAACCATGTCAGAAGAAGTCATGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

15.58

Weight (kDa)

10.5

Isoelectric Point (pI)

58.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019325)

Species Orthologous Gene IDs
pyrus_communis pycom12g06750
rosa_laevigata RLG00000014837
rosa_roxburghii Rroxscaffold_2G00117710
rosa_samantha Rh3DG349100 Rh5AG331100 Rh5BG273900 Rh5DG281800 Rh6CG333500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 1 cut(s) 275
AciI CCGC 1 cut(s) 366
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 248
AflII CTTAAG 2 cut(s) 16, 71
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 1 cut(s) 371
AluI AGCT 1 cut(s) 371
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 403
AoxI GGCC 2 cut(s) 121, 323
ApeKI GCWGC 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 78, 251
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 2 cut(s) 78, 251
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 403
BfrI CTTAAG 2 cut(s) 16, 71
BfuAI ACCTGC 1 cut(s) 58
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 78, 251
BmgT120I GGNCC 2 cut(s) 78, 251
BmrFI CCNGG 1 cut(s) 33
BmsI GCATC 3 cut(s) 151, 173, 212
BoxI GACNNNNGTC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 180, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 253, 293
BseBI CCWGG 1 cut(s) 33
BseGI GGATG 2 cut(s) 143, 166
BseLI CCNNNNNNNGG 1 cut(s) 248
BseNI ACTGG 2 cut(s) 253, 293
BseXI GCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 36
BshFI GGCC 2 cut(s) 123, 325
BsiHKAI GWGCWC 1 cut(s) 196
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 403
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 2 cut(s) 123, 325
Bsp1286I GDGCHC 1 cut(s) 196
BspACI CCGC 1 cut(s) 366
BspANI GGCC 2 cut(s) 123, 325
BspMI ACCTGC 1 cut(s) 58
BspTI CTTAAG 2 cut(s) 16, 71
BsrBI CCGCTC 1 cut(s) 366
BsrI ACTGG 2 cut(s) 253, 293
Bst2UI CCWGG 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 406
BstAFI CTTAAG 2 cut(s) 16, 71
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 2 cut(s) 143, 166
BstMAI GTCTC 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 161, 200
BstNI CCWGG 1 cut(s) 33
BstPAI GACNNNNGTC 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 182
BsuRI GGCC 2 cut(s) 123, 325
BtsCI GGATG 2 cut(s) 143, 166
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 2 cut(s) 78, 251
Csp6I GTAC 1 cut(s) 228
CviAII CATG 3 cut(s) 189, 414, 429
CviJI RGCY 5 cut(s) 123, 155, 292, 325, 371
CviKI_1 RGCY 5 cut(s) 123, 155, 292, 325, 371
CviQI GTAC 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
Eco147I AGGCCT 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 78, 251
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 166
FaeI CATG 3 cut(s) 192, 417, 432
FaiI YATR 6 cut(s) 68, 190, 348, 350, 415, 430
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 284
FatI CATG 3 cut(s) 188, 413, 428
FauNDI CATATG 1 cut(s) 348
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 2 cut(s) 150, 173
Fsp4HI GCNGC 1 cut(s) 372
GluI GCNGC 1 cut(s) 372
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 2 cut(s) 123, 325
Hin1II CATG 3 cut(s) 192, 417, 432
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 3 cut(s) 105, 277, 315
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 3 cut(s) 61, 134, 420
Hpy188III TCNNGA 1 cut(s) 267
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 7, 53, 164, 352, 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 200
Hsp92II CATG 3 cut(s) 192, 417, 432
LmnI GCTCC 2 cut(s) 200, 368
LpnPI CCDG 8 cut(s) 18, 45, 63, 192, 201, 234, 306, 338
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 3 cut(s) 151, 173, 212
MbiI CCGCTC 1 cut(s) 366
MboII GAAGA 2 cut(s) 393, 433
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 1 cut(s) 114
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 5 cut(s) 69, 128, 336, 348, 415
Mph1103I ATGCAT 1 cut(s) 166
MseI TTAA 5 cut(s) 17, 72, 90, 96, 117
MslI CAYNNNNRTG 1 cut(s) 433
MspCI CTTAAG 2 cut(s) 16, 71
MspR9I CCNGG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 161, 200
NdeI CATATG 1 cut(s) 348
NlaIII CATG 3 cut(s) 192, 417, 432
NsiI ATGCAT 1 cut(s) 166
PaqCI CACCTGC 1 cut(s) 58
PceI AGGCCT 1 cut(s) 325
PfeI GAWTC 2 cut(s) 105, 315
PflMI CCANNNNNTGG 1 cut(s) 248
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 78
PshAI GACNNNNGTC 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspPI GGNCC 2 cut(s) 78, 251
PspPPI RGGWCCY 1 cut(s) 78
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 433
SalI GTCGAC 1 cut(s) 274
SaqAI TTAA 5 cut(s) 17, 72, 90, 96, 117
SatI GCNGC 1 cut(s) 372
Sau96I GGNCC 2 cut(s) 78, 251
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 196
SetI ASST 9 cut(s) 34, 52, 83, 181, 214, 220, 340, 363, 373
SfaNI GCATC 3 cut(s) 151, 173, 212
SinI GGWCC 2 cut(s) 78, 251
SmiMI CAYNNNNRTG 1 cut(s) 433
SmlI CTYRAG 4 cut(s) 16, 71, 195, 207
SmoI CTYRAG 4 cut(s) 16, 71, 195, 207
Sse9I AATT 1 cut(s) 114
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 31
TaqI TCGA 1 cut(s) 275
TasI AATT 1 cut(s) 114
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 2 cut(s) 105, 315
Tru1I TTAA 5 cut(s) 17, 72, 90, 96, 117
Tru9I TTAA 5 cut(s) 17, 72, 90, 96, 117
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 248
Vha464I CTTAAG 2 cut(s) 16, 71
VpaK11BI GGWCC 2 cut(s) 78, 251
XmiI GTMKAC 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.