Rh5DG281800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019325
Physical Location & Seq
Forward (+)
34670181 .. 34670926
746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG281800.1

Sequence Viewer

Length: 483 bp
ATGAATATAGCACCCTTGGAACCTCCAGCATATCGACCTCAACCAGGGAGGCCTAAAAAGTTAAGAATAAGAGAACCTGGAGAAGTAGAACCACCTGCACAATCAGAAACTATACTTAAGAGGACCCTCTTTATTAAAGTTAAATGTGGATTCTGTAAAATTAAAGGCCACAATGTCAGAGGATGTCCAAAGAAAAAGGCTGTGGATGCATCAAGTTCCCAACCTGATGGTTCATGTGCTCAAGGAGCATCTCAAGGTTCAGGTGCTAAAAGTACTGCGACAACACCAAAAACTGGTCCAAATAAATCATCAAGAAAGTCGACTCAATCTCAAAAGCCAGTTGTCCCAAAGACAATAGGAATCAAAAGGCCTCAATCTCAACCTCAAACACATCTGCAGGCAAAGGTGAGCGGAGCTGCAAAGAAACCAAGACAGACCAATGTCTCTTCCTCGCAACCATGTCAGAAGAAGTCATGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

160

Amino Acids

17.17

Weight (kDa)

10.56

Isoelectric Point (pI)

60.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019325)

Species Orthologous Gene IDs
pyrus_communis pycom12g06750
rosa_laevigata RLG00000014837
rosa_roxburghii Rroxscaffold_2G00117710
rosa_samantha Rh3DG349100 Rh5AG331100 Rh5BG273900 Rh5DG281800 Rh6CG333500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 293
AccBSI CCGCTC 1 cut(s) 411
AccI GTMKAC 1 cut(s) 320
AciI CCGC 1 cut(s) 411
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 2 cut(s) 44, 293
AflII CTTAAG 1 cut(s) 116
AjnI CCWGG 2 cut(s) 43, 76
AluBI AGCT 1 cut(s) 416
AluI AGCT 1 cut(s) 416
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 448
AoxI GGCC 3 cut(s) 50, 166, 368
ApeKI GCWGC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 123, 296
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 2 cut(s) 123, 296
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 403
BccI CCATC 1 cut(s) 221
BciT130I CCWGG 2 cut(s) 45, 78
BcoDI GTCTC 1 cut(s) 448
BfmI CTRYAG 1 cut(s) 395
BfrI CTTAAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 418
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 2 cut(s) 45, 78
Bme18I GGWCC 2 cut(s) 123, 296
BmgT120I GGNCC 2 cut(s) 123, 296
BmiI GGNNCC 2 cut(s) 21, 125
BmrFI CCNGG 2 cut(s) 45, 78
BmsI GCATC 3 cut(s) 196, 218, 257
BoxI GACNNNNGTC 1 cut(s) 440
BpmI CTGGAG 2 cut(s) 9, 99
BpuEI CTTGAG 2 cut(s) 225, 237
BsaJI CCNNGG 2 cut(s) 15, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 293
Bse1I ACTGG 2 cut(s) 298, 338
BseBI CCWGG 2 cut(s) 45, 78
BseDI CCNNGG 2 cut(s) 15, 44
BseGI GGATG 2 cut(s) 188, 211
BseLI CCNNNNNNNGG 2 cut(s) 44, 293
BseNI ACTGG 2 cut(s) 298, 338
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 81
BshFI GGCC 3 cut(s) 52, 168, 370
BsiHKAI GWGCWC 1 cut(s) 241
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 44, 293
BsmAI GTCTC 1 cut(s) 448
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 3 cut(s) 52, 168, 370
Bsp1286I GDGCHC 1 cut(s) 241
BspACI CCGC 1 cut(s) 411
BspANI GGCC 3 cut(s) 52, 168, 370
BspLI GGNNCC 2 cut(s) 21, 125
BspMAI CTGCAG 1 cut(s) 399
BspMI ACCTGC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 411
BsrI ACTGG 2 cut(s) 298, 338
BssECI CCNNGG 2 cut(s) 15, 44
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 45, 78
Bst6I CTCTTC 1 cut(s) 451
BstAFI CTTAAG 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 399
BstENI CCTNNNNNAGG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 188, 211
BstMAI GTCTC 1 cut(s) 448
BstMWI GCNNNNNNNGC 2 cut(s) 206, 245
BstNI CCWGG 2 cut(s) 45, 78
BstPAI GACNNNNGTC 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 43, 76
BstSFI CTRYAG 1 cut(s) 395
BstV1I GCAGC 1 cut(s) 403
BstXI CCANNNNNNTGG 1 cut(s) 227
BsuRI GGCC 3 cut(s) 52, 168, 370
BtsCI GGATG 2 cut(s) 188, 211
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 123, 296
Csp6I GTAC 1 cut(s) 273
CviAII CATG 3 cut(s) 234, 459, 474
CviJI RGCY 6 cut(s) 52, 168, 200, 337, 370, 416
CviKI_1 RGCY 6 cut(s) 52, 168, 200, 337, 370, 416
CviQI GTAC 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 15
Eco147I AGGCCT 2 cut(s) 52, 370
Eco47I GGWCC 2 cut(s) 123, 296
EcoNI CCTNNNNNAGG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 43, 76
EcoT14I CCWWGG 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 3 cut(s) 237, 462, 477
FaiI YATR 6 cut(s) 8, 31, 113, 235, 460, 475
FaqI GGGAC 1 cut(s) 329
FatI CATG 3 cut(s) 233, 458, 473
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 2 cut(s) 195, 218
Fsp4HI GCNGC 1 cut(s) 417
GluI GCNGC 1 cut(s) 417
GsuI CTGGAG 2 cut(s) 9, 99
HaeIII GGCC 3 cut(s) 52, 168, 370
Hin1II CATG 3 cut(s) 237, 462, 477
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HinfI GANTC 3 cut(s) 150, 322, 360
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 321
Hpy188I TCNGA 3 cut(s) 106, 179, 465
Hpy188III TCNNGA 1 cut(s) 312
Hpy8I GTNNAC 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 98, 209, 397, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 245
Hsp92II CATG 3 cut(s) 237, 462, 477
LmnI GCTCC 2 cut(s) 245, 413
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 3 cut(s) 196, 218, 257
MbiI CCGCTC 1 cut(s) 411
MboII GAAGA 2 cut(s) 438, 478
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 9 cut(s) 33, 42, 48, 114, 137, 173, 381, 393, 460
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 5 cut(s) 62, 117, 135, 141, 162
MslI CAYNNNNRTG 1 cut(s) 478
MspCI CTTAAG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 45, 78
MvaI CCWGG 2 cut(s) 45, 78
MwoI GCNNNNNNNGC 2 cut(s) 206, 245
NlaIII CATG 3 cut(s) 237, 462, 477
NlaIV GGNNCC 2 cut(s) 21, 125
NsiI ATGCAT 1 cut(s) 211
PaqCI CACCTGC 1 cut(s) 103
PceI AGGCCT 2 cut(s) 52, 370
PfeI GAWTC 2 cut(s) 150, 360
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 123
PshAI GACNNNNGTC 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 43, 76
PspGI CCWGG 2 cut(s) 43, 76
PspN4I GGNNCC 2 cut(s) 21, 125
PspPI GGNCC 2 cut(s) 123, 296
PspPPI RGGWCCY 1 cut(s) 123
PstI CTGCAG 1 cut(s) 399
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 478
SalI GTCGAC 1 cut(s) 319
SaqAI TTAA 5 cut(s) 62, 117, 135, 141, 162
SatI GCNGC 1 cut(s) 417
Sau96I GGNCC 2 cut(s) 123, 296
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 45, 78
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 3 cut(s) 196, 218, 257
SfcI CTRYAG 1 cut(s) 395
SinI GGWCC 2 cut(s) 123, 296
SmiMI CAYNNNNRTG 1 cut(s) 478
SmlI CTYRAG 3 cut(s) 116, 240, 252
SmoI CTYRAG 3 cut(s) 116, 240, 252
Sse9I AATT 1 cut(s) 159
SseBI AGGCCT 2 cut(s) 52, 370
SsiI CCGC 1 cut(s) 411
StuI AGGCCT 2 cut(s) 52, 370
StyD4I CCNGG 2 cut(s) 43, 76
StyI CCWWGG 1 cut(s) 15
TaqI TCGA 2 cut(s) 34, 320
TasI AATT 1 cut(s) 159
TatI WGTACW 1 cut(s) 272
TfiI GAWTC 2 cut(s) 150, 360
Tru1I TTAA 5 cut(s) 62, 117, 135, 141, 162
Tru9I TTAA 5 cut(s) 62, 117, 135, 141, 162
TseI GCWGC 1 cut(s) 416
TspDTI ATGAA 2 cut(s) 17, 222
Van91I CCANNNNNTGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 123, 296
XagI CCTNNNNNAGG 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 320
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.