Rh5AG331100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019325
Physical Location & Seq
Forward (+)
52500482 .. 52519375
18894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG331100.1

Sequence Viewer

Length: 807 bp
ATGAATAAAGAGGAAGTGAATAATGGTGTGGAAAGGATCCAAGTTATGGCTGATTTTGAAGTGTCTAAGAATGATGAGTTGGCTATTGTTGAAGTGTCACCAAATGGTTTTGTGGGAGATACTAATGATTTGGTAGAGAATGACTATGCTATAGAGAATGATGATGTCGAAGGATATGTGGCAGATGATGACTTATTAATGGAAAAAAGTGCAAAAGTTAAGAAGGCTGTAAAAGGCAAAGCTAAAGTTGTTGATGGAGACAAATCTAAAAAAGCTAAGGGAAGAAAGTGTGCAAAAGCAAAGCAATACAACACTCGATTCAAGGGAGAGAAATCATTTGAAGAAGTTGACTTGGAAGATCAGACAACTGATGAGGAAGACAAAGAATTCTTTATAGAGAGTGATTTTGAGCAGAACAATGCAGATGATGAAATCTTCTTTGAAGAGGAGATAGAAGAACCAAATGCCATTGAAGAGTTTGCTGAAATGGGCCACAATGTCAGAGGATGTCCAAAGAAAAAGGCTGTGGATGCATCAAGTTCCCAACCTGATGGTTCATGTGCTCAAGGAGCATCTCAAGGTTCAGGTACTAAAAGTACTGCGACAACACCAAAAACTGGTCCAAATAAATCATCAAGAAAGTCGACTCAATCTCAAAAGCCAGCTGTCCCAAAGACAATAGGAATCAAAAGGCCTCAATCTCAACCTCAAACACATCTGCAGGCAAAGGTGAGCGGAGCTGCAAAGAAACCAAGACAGACCAATGTCTCTTCCTCGCAACCATGTCAGAAGAAGTCATGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.45

Weight (kDa)

5.23

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019325)

Species Orthologous Gene IDs
pyrus_communis pycom12g06750
rosa_laevigata RLG00000014837
rosa_roxburghii Rroxscaffold_2G00117710
rosa_samantha Rh3DG349100 Rh5AG331100 Rh5BG273900 Rh5DG281800 Rh6CG333500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 46, 617
AccBSI CCGCTC 1 cut(s) 735
AccI GTMKAC 1 cut(s) 644
AciI CCGC 1 cut(s) 735
AclWI GGATC 2 cut(s) 31, 44
AcsI RAATTY 1 cut(s) 386
AfaI GTAC 2 cut(s) 589, 598
AfiI CCNNNNNNNGG 2 cut(s) 46, 617
AgsI TTSAA 6 cut(s) 59, 92, 322, 341, 443, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 4 cut(s) 242, 275, 665, 740
AluI AGCT 4 cut(s) 242, 275, 665, 740
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 2 cut(s) 252, 772
AlwI GGATC 2 cut(s) 31, 44
AoxI GGCC 2 cut(s) 490, 692
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 1 cut(s) 386
AseI ATTAAT 1 cut(s) 197
AspS9I GGNCC 2 cut(s) 490, 620
AsuHPI GGTGA 2 cut(s) 90, 742
AvaII GGWCC 1 cut(s) 620
BamHI GGATCC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 1 cut(s) 727
BccI CCATC 2 cut(s) 248, 545
BcoDI GTCTC 2 cut(s) 252, 772
BfmI CTRYAG 2 cut(s) 150, 719
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
BmcAI AGTACT 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 2 cut(s) 490, 620
BmiI GGNNCC 1 cut(s) 38
BmsI GCATC 3 cut(s) 520, 542, 581
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 384
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 549, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 617
Bse1I ACTGG 1 cut(s) 622
BseGI GGATG 2 cut(s) 512, 535
BseLI CCNNNNNNNGG 2 cut(s) 46, 617
BseNI ACTGG 1 cut(s) 622
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 1 cut(s) 727
BshFI GGCC 2 cut(s) 492, 694
BsiHKAI GWGCWC 1 cut(s) 565
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 2 cut(s) 46, 617
BsmAI GTCTC 2 cut(s) 252, 772
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 2 cut(s) 492, 694
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 2 cut(s) 36, 358
BspACI CCGC 1 cut(s) 735
BspANI GGCC 2 cut(s) 492, 694
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 723
BspPI GGATC 2 cut(s) 31, 44
BsrBI CCGCTC 1 cut(s) 735
BsrI ACTGG 1 cut(s) 622
BssMI GATC 2 cut(s) 36, 358
Bst6I CTCTTC 3 cut(s) 438, 468, 775
BstC8I GCNNGC 2 cut(s) 663, 723
BstDEI CTNAG 2 cut(s) 66, 276
BstF5I GGATG 2 cut(s) 512, 535
BstKTI GATC 2 cut(s) 39, 361
BstMAI GTCTC 2 cut(s) 252, 772
BstMBI GATC 2 cut(s) 36, 358
BstMWI GCNNNNNNNGC 2 cut(s) 530, 569
BstPAI GACNNNNGTC 1 cut(s) 764
BstSFI CTRYAG 2 cut(s) 150, 719
BstV1I GCAGC 1 cut(s) 727
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 551
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 2 cut(s) 492, 694
BtsCI GGATG 2 cut(s) 512, 535
Cac8I GCNNGC 2 cut(s) 663, 723
Cfr13I GGNCC 2 cut(s) 490, 620
Csp6I GTAC 2 cut(s) 588, 597
CviAII CATG 3 cut(s) 558, 783, 798
CviQI GTAC 2 cut(s) 588, 597
DdeI CTNAG 2 cut(s) 66, 276
DpnI GATC 2 cut(s) 38, 360
DpnII GATC 2 cut(s) 36, 358
Eam1104I CTCTTC 3 cut(s) 438, 468, 775
EarI CTCTTC 3 cut(s) 438, 468, 775
Eco147I AGGCCT 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 535
FaeI CATG 3 cut(s) 561, 786, 801
FaiI YATR 8 cut(s) 47, 147, 152, 177, 395, 559, 784, 799
FaqI GGGAC 1 cut(s) 653
FatI CATG 3 cut(s) 557, 782, 797
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 2 cut(s) 519, 542
Fsp4HI GCNGC 1 cut(s) 741
GluI GCNGC 1 cut(s) 741
HaeIII GGCC 2 cut(s) 492, 694
Hin1II CATG 3 cut(s) 561, 786, 801
HincII GTYRAC 2 cut(s) 349, 645
HindII GTYRAC 2 cut(s) 349, 645
HinfI GANTC 3 cut(s) 318, 646, 684
HphI GGTGA 2 cut(s) 90, 742
Hpy166II GTNNAC 2 cut(s) 349, 645
Hpy188I TCNGA 3 cut(s) 363, 503, 789
Hpy188III TCNNGA 1 cut(s) 636
Hpy8I GTNNAC 2 cut(s) 349, 645
HpyAV CCTTC 2 cut(s) 164, 217
HpyCH4V TGCA 6 cut(s) 212, 293, 422, 533, 721, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 530, 569
HpyF3I CTNAG 2 cut(s) 66, 276
Hsp92II CATG 3 cut(s) 561, 786, 801
Kzo9I GATC 2 cut(s) 36, 358
LmnI GCTCC 2 cut(s) 569, 737
LpnPI CCDG 5 cut(s) 561, 570, 603, 675, 707
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 3 cut(s) 520, 542, 581
MaeIII GTNAC 1 cut(s) 96
MalI GATC 2 cut(s) 38, 360
MbiI CCGCTC 1 cut(s) 735
MboI GATC 2 cut(s) 36, 358
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 1 cut(s) 386
MlyI GAGTC 1 cut(s) 640
MnlI CCTC 7 cut(s) 4, 367, 439, 497, 705, 717, 784
Mph1103I ATGCAT 1 cut(s) 535
MseI TTAA 2 cut(s) 197, 219
MslI CAYNNNNRTG 1 cut(s) 802
MspA1I CMGCKG 1 cut(s) 665
MwoI GCNNNNNNNGC 2 cut(s) 530, 569
NdeII GATC 2 cut(s) 36, 358
NlaIII CATG 3 cut(s) 561, 786, 801
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 96
NsiI ATGCAT 1 cut(s) 535
PceI AGGCCT 1 cut(s) 694
PfeI GAWTC 2 cut(s) 318, 684
PflMI CCANNNNNTGG 2 cut(s) 46, 617
PkrI GCNGC 1 cut(s) 742
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PshAI GACNNNNGTC 1 cut(s) 764
PshBI ATTAAT 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 2 cut(s) 490, 620
PstI CTGCAG 1 cut(s) 723
PsuI RGATCY 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 2 cut(s) 589, 598
RsaNI GTAC 2 cut(s) 588, 597
RseI CAYNNNNRTG 1 cut(s) 802
SalI GTCGAC 1 cut(s) 643
SaqAI TTAA 2 cut(s) 197, 219
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 2 cut(s) 36, 358
Sau96I GGNCC 2 cut(s) 490, 620
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 1 cut(s) 640
SduI GDGCHC 1 cut(s) 565
SetI ASST 9 cut(s) 244, 277, 550, 583, 589, 667, 709, 732, 742
SfaNI GCATC 3 cut(s) 520, 542, 581
SfcI CTRYAG 2 cut(s) 150, 719
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 1 cut(s) 802
SmlI CTYRAG 2 cut(s) 564, 576
SmoI CTYRAG 2 cut(s) 564, 576
Sse9I AATT 1 cut(s) 386
SseBI AGGCCT 1 cut(s) 694
SsiI CCGC 1 cut(s) 735
StuI AGGCCT 1 cut(s) 694
TaqI TCGA 3 cut(s) 168, 316, 644
TasI AATT 1 cut(s) 386
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 2 cut(s) 318, 684
Tru1I TTAA 2 cut(s) 197, 219
Tru9I TTAA 2 cut(s) 197, 219
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 17, 444, 546
Van91I CCANNNNNTGG 2 cut(s) 46, 617
VpaK11BI GGWCC 1 cut(s) 620
VspI ATTAAT 1 cut(s) 197
XapI RAATTY 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 644
ZrmI AGTACT 1 cut(s) 598
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.