Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0007627

Physical Location & Seq

Genomic Coordinates Forward (+)

60402450 .. 60408951

Gene Structure 6502 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014996

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGGAGCTTCAGAGATCAGCGGTGGGACTCTTTCTAGGGTTTCTGGTGCTGTCTGTGGTGCCTTTATCGATGGGGAGGTTCGTGGTGGAGAAGAACAGCTTGAGGGTGACGTCACCGGATAAAATCAAGGGGAAATATGACAGTGCCATTGGCAACTTTGGGCTTCCTCAGTATGGAGGGAGCATGTCTGGTGCTGTTATATATCCAAAGGAGAATCAGAAGGGGTGTAAGGAGTTCTCGGAGTTTGGGCTTTCCTACAAATCCGATCCCGGAGCTCTGCCGACGTTTCTGTTGGTCGATCGTGGAGATTGCTTCTTTGCCTTGAAGGTTTGGAATGCCCAGAAGGCTGGAGCTTCTGCAGTCCTTGTCGCTGATGATATTGAGGAGGCGTTAATAACTATGGACACACCAGAAGAAGATATTTCAACTGCCAAATACATTGAAAACATAACTATACCATCTGCACTCATTGAAAAAAGTTTTGGTCAAACATTGAAGAAGGCCATCAGTTCTGGTGATATGGTGAATGTGTACCTTGACTGGAGAGAATCTGTTCCTCACCCAGATGATCGTGTGGAATATGAACTATGGACCAACAGTAATGATGAATGTGGGGTTAAGTGTGACATGTTGATGGAATTTACAAAGGAGTTTAGGGGTGCAGCCCAGATACTTGAAAAAGGGGGTTACACTCAGTTCACACCGCATTATATAACTTGGTACTGTCCTAAGGCATTCACCTTGAGCAAACAGTGCAAATCTCAGTGTATCAATCATGGAAGATATTGTGCTCCTGACCCTGAACAAGACTTCAGCACTGGTTACGAAGGGAAAGATGTGGTTCTTGAAAATTTAAGGCAGCTGTGTGTTTTTAAAGTAGCAAATGAGACTCGAAAGCCTTGGGTATGGTGGGACTATGTGACTGATTTTCAAATCAGATGTCCAATGAAAGAGAAAAAATACAACAAGGACTGTGCTGATGCTGTCATTAAGTCTCTCGGGCTTGATAGTAAGAAGATCGACAAGTGTATGGGAAACTCAGATGCAGACTCTGAAAATCCTGTTCTGAAAGAAGAGCAAGATGCTCAGGTTGGGAAGGGAACAAGAGGTGATGTAACCATATTGCCTACACTGGTTGTCAATAATCGACAATACCGAGGAAAGTTGGCAAAAAGTGCAGTTCTGAAGGCCATCTGTTCCGGTTTTGAAGAAACTACTGAGCCAGCTGTTTGCTTGAGTACTGATGTTGAGACAAATGAGTGTTTGGACAATAATGGAGGTTGCTGGCAAAATAAAGCTGCCAACATTACAGCCTGCAAGGATACCTTTCGTGGGAGGGTATGTGAGTGCCCTCTCGTTGATGGTGTGCAGTTCAAAGGAGATGGTTACACTACCTGTGAAGCAAGTGGGCCTGGGAGGTGCAAGGTAAACAATGGAGGTTGTTGGCATGAAGCTCGAGATGGGCATGCATTCTCTGCTTGTACAGATGATGGAGGGGTGCAATGCAGGTGTCCTCCTGGGTTTATAGGTGACGGTGTGAAAACTTGTGAAGATGTCGATGAATGCAAAGAGAAGAAAGCCTGTCAGTGCCCTGAATGCTCCTGTAAAAATACCTGGGGAAGTTATGACTGCTCATGTAGTGGAAATCTTTTGTATATCAAAGATCATGACACCTGCATAAGCAAGACTTCTGGAGGATCAAAATCTTCTGCATGGGCTGCTGTGTGGGTTATTTTAATTGGCTTGGCTATGGCTTCTTGTGGGGCATATCTTGTGTACAAATATCGATTACGGTCATACATGGATTCAGAAATTAGAGCTATAATGGCACAGTATATGCCTCTGGACAGCCAAGCTGAAGTTCCCAATCATGTGAATGATGAGCATGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005794 GO:0005802 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0031410 GO:0031982 GO:0031984 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

631

Amino Acids

69.54

Weight (kDa)

5.25

Isoelectric Point (pI)

35.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	63 - 165	6.4e-14	PA domain
VSR_TRX	PF25011	192 - 399	3.2e-104	Vacuolar sorting receptor thioredoxin-like domain
cEGF	PF12662	500 - 521	1.3e-07	Complement Clr-like EGF-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007627)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G14720 AT2G14720 AT2G14740 AT2G14740 AT3G52850
fragaria_vesca	FvH4_2g04230 FvH4_2g04230 FvH4_2g04230
malus_domestica	MD05G1099000.v1.1 MD10G1105000.v1.1
prunus_persica	Prupe.8G143700_v2.0.a1 Prupe.8G143700_v2.0.a1
pyrus_communis	pycom05g09820 pycom10g09040
rosa_chinensis	RchiOBHm_Chr6g0253651
rosa_laevigata	RLG00000014996
rosa_multiflora	Rmu_sc0000331.1_g000012
rosa_roxburghii	Rroxscaffold_7G00211730
rosa_rugosa	Rorug05G0545300
rosa_samantha	Rh6AG061000 Rh6BG054800 Rh6CG054400 Rh6DG051900
rosa_wichuraiana	Rw6G005420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1497, 1682
AatII	GACGTC	1 cut(s)	113
Acc36I	ACCTGC	2 cut(s)	1497, 1682
AccB1I	GGYRCC	1 cut(s)	58
AciI	CCGC	2 cut(s)	20, 704
AclWI	GGATC	2 cut(s)	260, 1705
AcsI	RAATTY	2 cut(s)	638, 850
AcuI	CTGAAG	3 cut(s)	796, 1205, 1878
AcyI	GRCGYC	1 cut(s)	110
AfaI	GTAC	5 cut(s)	533, 722, 1240, 1483, 1778
AfiI	CCNNNNNNNGG	2 cut(s)	173, 1332
AflIII	ACRYGT	1 cut(s)	627
AjnI	CCWGG	3 cut(s)	1411, 1516, 1613
AjuI	GAANNNNNNNTTGG	4 cut(s)	465, 497, 1845, 1877
Alw21I	GWGCWC	2 cut(s)	277, 793
Alw26I	GTCTC	3 cut(s)	881, 999, 1244
AlwI	GGATC	2 cut(s)	260, 1705
AlwNI	CAGNNNCTG	1 cut(s)	1052
Ama87I	CYCGRG	2 cut(s)	998, 1455
AoxI	GGCC	3 cut(s)	501, 1188, 1409
ApeKI	GCWGC	4 cut(s)	662, 859, 1298, 1718
ApoI	RAATTY	2 cut(s)	638, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	925, 957
Asp700I	GAANNNNTTC	1 cut(s)	552
AspS9I	GGNCC	2 cut(s)	591, 1409
AsuC2I	CCSGG	1 cut(s)	270
AsuHPI	GGTGA	8 cut(s)	105, 118, 527, 535, 551, 730, 1121, 1541
AvaI	CYCGRG	2 cut(s)	998, 1455
AvaII	GGWCC	1 cut(s)	591
AxyI	CCTNAGG	1 cut(s)	729
BaeGI	GKGCMC	2 cut(s)	1352, 1592
BanI	GGYRCC	1 cut(s)	58
BanII	GRGCYC	1 cut(s)	277
Bbv12I	GWGCWC	2 cut(s)	277, 793
BbvI	GCAGC	4 cut(s)	674, 871, 1285, 1705
BccI	CCATC	9 cut(s)	64, 466, 512, 628, 1199, 1355, 1376, 1454, 1484
BcgI	CGANNNNNNTGC	2 cut(s)	1436, 1470
BciT130I	CCWGG	3 cut(s)	1413, 1518, 1615
BciVI	GTATCC	1 cut(s)	1315
BcnI	CCSGG	1 cut(s)	270
BcoDI	GTCTC	3 cut(s)	881, 999, 1244
BfaI	CTAG	1 cut(s)	35
BfmI	CTRYAG	1 cut(s)	357
BfuAI	ACCTGC	2 cut(s)	1497, 1682
BfuI	GTATCC	1 cut(s)	1315
BglI	GCCNNNNNGGC	1 cut(s)	344
BisI	GCNGC	4 cut(s)	663, 860, 1299, 1719
BlsI	GCNGC	4 cut(s)	664, 861, 1300, 1720
BmcAI	AGTACT	1 cut(s)	1240
Bme1390I	CCNGG	4 cut(s)	270, 1413, 1518, 1615
Bme18I	GGWCC	1 cut(s)	591
BmeT110I	CYCGRG	2 cut(s)	998, 1455
BmgT120I	GGNCC	2 cut(s)	591, 1409
BmiI	GGNNCC	1 cut(s)	60
BmrFI	CCNGG	4 cut(s)	270, 1413, 1518, 1615
BmsI	GCATC	3 cut(s)	970, 1033, 1072
BpmI	CTGGAG	3 cut(s)	369, 562, 1713
Bpu10I	CCTNAGC	1 cut(s)	1086
BpuEI	CTTGAG	3 cut(s)	121, 763, 1255
BpuMI	CCSGG	1 cut(s)	270
Bsa29I	ATCGAT	2 cut(s)	68, 1786
BsaBI	GATNNNNATC	1 cut(s)	1702
BsaHI	GRCGYC	1 cut(s)	110
BsaJI	CCNNGG	5 cut(s)	899, 1156, 1412, 1517, 1614
BsaWI	WCCGGW	2 cut(s)	115, 1199
BsaXI	ACNNNNNCTCC	2 cut(s)	1327, 1357
Bsc4I	CCNNNNNNNGG	2 cut(s)	173, 1332
Bse1I	ACTGG	3 cut(s)	545, 823, 1137
Bse21I	CCTNAGG	1 cut(s)	729
Bse3DI	GCAATG	1 cut(s)	1508
Bse8I	GATNNNNATC	1 cut(s)	1702
BseBI	CCWGG	3 cut(s)	1413, 1518, 1615
BseCI	ATCGAT	2 cut(s)	68, 1786
BseDI	CCNNGG	5 cut(s)	899, 1156, 1412, 1517, 1614
BseJI	GATNNNNATC	1 cut(s)	1702
BseLI	CCNNNNNNNGG	2 cut(s)	173, 1332
BseMI	GCAATG	1 cut(s)	1508
BseMII	CTCAG	6 cut(s)	182, 707, 776, 1053, 1100, 1209
BseNI	ACTGG	3 cut(s)	545, 823, 1137
BseRI	GAGGAG	1 cut(s)	398
BseSI	GKGCMC	2 cut(s)	1352, 1592
BseXI	GCAGC	4 cut(s)	674, 871, 1285, 1705
BsgI	GTGCAG	4 cut(s)	447, 681, 1197, 1388
Bsh1285I	CGRYCG	1 cut(s)	301
BshFI	GGCC	3 cut(s)	503, 1190, 1411
BshNI	GGYRCC	1 cut(s)	58
BshVI	ATCGAT	2 cut(s)	68, 1786
BsiEI	CGRYCG	1 cut(s)	301
BsiHKAI	GWGCWC	2 cut(s)	277, 793
BsiHKCI	CYCGRG	2 cut(s)	998, 1455
BsiSI	CCGG	3 cut(s)	116, 270, 1200
BslFI	GGGAC	2 cut(s)	39, 926
BslI	CCNNNNNNNGG	2 cut(s)	173, 1332
BsmAI	GTCTC	3 cut(s)	881, 999, 1244
BsmFI	GGGAC	2 cut(s)	39, 926
BsmI	GAATGC	5 cut(s)	340, 734, 1469, 1568, 1601
BsnI	GGCC	3 cut(s)	503, 1190, 1411
BsoBI	CYCGRG	2 cut(s)	998, 1455
Bsp1286I	GDGCHC	4 cut(s)	277, 793, 1352, 1592
Bsp1407I	TGTACA	2 cut(s)	1481, 1776
Bsp143I	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
BspACI	CCGC	2 cut(s)	20, 704
BspANI	GGCC	3 cut(s)	503, 1190, 1411
BspCNI	CTCAG	6 cut(s)	181, 706, 775, 1052, 1099, 1210
BspDI	ATCGAT	2 cut(s)	68, 1786
BspHI	TCATGA	1 cut(s)	1666
BspLI	GGNNCC	1 cut(s)	60
BspMAI	CTGCAG	1 cut(s)	361
BspMI	ACCTGC	2 cut(s)	1497, 1682
BspPI	GGATC	2 cut(s)	260, 1705
BspQI	GCTCTTC	1 cut(s)	1068
BspT107I	GGYRCC	1 cut(s)	58
BsrDI	GCAATG	1 cut(s)	1508
BsrGI	TGTACA	2 cut(s)	1481, 1776
BsrI	ACTGG	3 cut(s)	545, 823, 1137
BssECI	CCNNGG	5 cut(s)	899, 1156, 1412, 1517, 1614
BssMI	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
BssNI	GRCGYC	1 cut(s)	110
BssT1I	CCWWGG	1 cut(s)	899
Bst2UI	CCWGG	3 cut(s)	1413, 1518, 1615
Bst4CI	ACNGT	8 cut(s)	143, 599, 725, 753, 974, 1535, 1794, 1833
Bst6I	CTCTTC	1 cut(s)	1068
BstACI	GRCGYC	1 cut(s)	110
BstAPI	GCANNNNNTGC	4 cut(s)	753, 1175, 1475, 1718
BstAUI	TGTACA	2 cut(s)	1481, 1776
BstC8I	GCNNGC	5 cut(s)	1224, 1286, 1315, 1467, 1887
BstDEI	CTNAG	7 cut(s)	168, 693, 729, 762, 1039, 1086, 1218
BstKTI	GATC	7 cut(s)	17, 268, 301, 571, 1020, 1666, 1700
BstMAI	GTCTC	3 cut(s)	881, 999, 1244
BstMBI	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
BstMCI	CGRYCG	1 cut(s)	301
BstMWI	GCNNNNNNNGC	7 cut(s)	344, 753, 1175, 1475, 1596, 1718, 1826
BstNI	CCWGG	3 cut(s)	1413, 1518, 1615
BstNSI	RCATGY	4 cut(s)	187, 631, 1469, 1889
BstSCI	CCNGG	4 cut(s)	268, 1411, 1516, 1613
BstSFI	CTRYAG	1 cut(s)	357
BstSLI	GKGCMC	2 cut(s)	1352, 1592
BstV1I	GCAGC	4 cut(s)	674, 871, 1285, 1705
BstXI	CCANNNNNNTGG	1 cut(s)	347
Bsu15I	ATCGAT	2 cut(s)	68, 1786
Bsu36I	CCTNAGG	1 cut(s)	729
BsuI	GTATCC	1 cut(s)	1315
BsuRI	GGCC	3 cut(s)	503, 1190, 1411
BsuTUI	ATCGAT	2 cut(s)	68, 1786
BtsIMutI	CAGTG	6 cut(s)	148, 758, 770, 816, 1130, 1592
BveI	ACCTGC	2 cut(s)	1497, 1682
Cac8I	GCNNGC	5 cut(s)	1224, 1286, 1315, 1467, 1887
CaiI	CAGNNNCTG	1 cut(s)	1052
CciI	TCATGA	1 cut(s)	1666
Cfr13I	GGNCC	2 cut(s)	591, 1409
ClaI	ATCGAT	2 cut(s)	68, 1786
Csp6I	GTAC	5 cut(s)	532, 721, 1239, 1482, 1777
CviQI	GTAC	5 cut(s)	532, 721, 1239, 1482, 1777
DdeI	CTNAG	7 cut(s)	168, 693, 729, 762, 1039, 1086, 1218
DpnI	GATC	7 cut(s)	16, 267, 300, 570, 1019, 1665, 1699
DpnII	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
DraI	TTTAAA	1 cut(s)	874
Eam1104I	CTCTTC	1 cut(s)	1068
EarI	CTCTTC	1 cut(s)	1068
Ecl136II	GAGCTC	1 cut(s)	275
Eco130I	CCWWGG	1 cut(s)	899
Eco24I	GRGCYC	1 cut(s)	277
Eco47I	GGWCC	1 cut(s)	591
Eco53kI	GAGCTC	1 cut(s)	275
Eco57I	CTGAAG	3 cut(s)	796, 1205, 1878
Eco81I	CCTNAGG	1 cut(s)	729
Eco88I	CYCGRG	2 cut(s)	998, 1455
EcoICRI	GAGCTC	1 cut(s)	275
EcoRII	CCWGG	3 cut(s)	1411, 1516, 1613
EcoT14I	CCWWGG	1 cut(s)	899
EcoT22I	ATGCAT	1 cut(s)	1471
EcoT38I	GRGCYC	1 cut(s)	277
ErhI	CCWWGG	1 cut(s)	899
FalI	AAGNNNNNCTT	6 cut(s)	83, 115, 1310, 1342, 1672, 1704
FaqI	GGGAC	2 cut(s)	39, 926
Fnu4HI	GCNGC	4 cut(s)	663, 860, 1299, 1719
FriOI	GRGCYC	1 cut(s)	277
Fsp4HI	GCNGC	4 cut(s)	663, 860, 1299, 1719
FspBI	CTAG	1 cut(s)	35
GluI	GCNGC	4 cut(s)	663, 860, 1299, 1719
GsuI	CTGGAG	3 cut(s)	369, 562, 1713
HaeIII	GGCC	3 cut(s)	503, 1190, 1411
HapII	CCGG	3 cut(s)	116, 270, 1200
Hin1I	GRCGYC	1 cut(s)	110
HinfI	GANTC	6 cut(s)	27, 214, 548, 889, 1049, 1805
HpaII	CCGG	3 cut(s)	116, 270, 1200
HphI	GGTGA	8 cut(s)	105, 118, 527, 535, 551, 730, 1121, 1541
Hpy166II	GTNNAC	4 cut(s)	532, 699, 1429, 1777
Hpy188III	TCNNGA	6 cut(s)	794, 845, 1457, 1667, 1692, 1844
Hpy8I	GTNNAC	4 cut(s)	532, 699, 1429, 1777
Hpy99I	CGWCG	1 cut(s)	286
HpyAV	CCTTC	7 cut(s)	214, 319, 337, 493, 821, 1090, 1180
HpyCH4III	ACNGT	8 cut(s)	143, 599, 725, 753, 974, 1535, 1794, 1833
HpyCH4IV	ACGT	2 cut(s)	110, 284
HpyF10VI	GCNNNNNNNGC	7 cut(s)	344, 753, 1175, 1475, 1596, 1718, 1826
HpyF3I	CTNAG	7 cut(s)	168, 693, 729, 762, 1039, 1086, 1218
HpySE526I	ACGT	2 cut(s)	110, 284
Hsp92I	GRCGYC	1 cut(s)	110
Kzo9I	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
LguI	GCTCTTC	1 cut(s)	1068
LmnI	GCTCC	6 cut(s)	4, 180, 272, 350, 796, 1604
Lsp1109I	GCAGC	4 cut(s)	674, 871, 1285, 1705
LweI	GCATC	3 cut(s)	970, 1033, 1072
MaeI	CTAG	1 cut(s)	35
MaeII	ACGT	2 cut(s)	110, 284
MaeIII	GTNAC	9 cut(s)	106, 111, 623, 686, 821, 919, 1114, 1385, 1529
MalI	GATC	7 cut(s)	16, 267, 300, 570, 1019, 1665, 1699
MboI	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
MhlI	GDGCHC	4 cut(s)	277, 793, 1352, 1592
MluCI	AATT	4 cut(s)	638, 850, 1737, 1812
MlyI	GAGTC	3 cut(s)	21, 883, 1043
Mph1103I	ATGCAT	1 cut(s)	1471
MroXI	GAANNNNTTC	1 cut(s)	552
MseI	TTAA	6 cut(s)	392, 618, 854, 873, 990, 1736
MslI	CAYNNNNRTG	3 cut(s)	564, 632, 1875
MspA1I	CMGCKG	3 cut(s)	20, 862, 1226
MspI	CCGG	3 cut(s)	116, 270, 1200
MspR9I	CCNGG	4 cut(s)	270, 1413, 1518, 1615
Mva1269I	GAATGC	5 cut(s)	340, 734, 1469, 1568, 1601
MvaI	CCWGG	3 cut(s)	1413, 1518, 1615
MwoI	GCNNNNNNNGC	7 cut(s)	344, 753, 1175, 1475, 1596, 1718, 1826
NciI	CCSGG	1 cut(s)	270
NdeII	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
NlaIV	GGNNCC	1 cut(s)	60
NmuCI	GTSAC	5 cut(s)	106, 111, 623, 919, 1529
NsiI	ATGCAT	1 cut(s)	1471
NspI	RCATGY	4 cut(s)	187, 631, 1469, 1889
PaeI	GCATGC	2 cut(s)	1469, 1889
PaeR7I	CTCGAG	1 cut(s)	1455
PagI	TCATGA	1 cut(s)	1666
PaqCI	CACCTGC	2 cut(s)	1497, 1682
PciI	ACATGT	1 cut(s)	627
PciSI	GCTCTTC	1 cut(s)	1068
PcsI	WCGNNNNNNNCGW	1 cut(s)	1153
PctI	GAATGC	5 cut(s)	340, 734, 1469, 1568, 1601
PdmI	GAANNNNTTC	1 cut(s)	552
PfeI	GAWTC	3 cut(s)	214, 548, 1805
PfoI	TCCNGGA	1 cut(s)	268
PkrI	GCNGC	4 cut(s)	664, 861, 1300, 1720
Ple19I	CGATCG	1 cut(s)	301
PleI	GAGTC	3 cut(s)	21, 883, 1043
PpsI	GAGTC	3 cut(s)	21, 883, 1043
PscI	ACATGT	1 cut(s)	627
Psp124BI	GAGCTC	1 cut(s)	277
Psp6I	CCWGG	3 cut(s)	1411, 1516, 1613
PspGI	CCWGG	3 cut(s)	1411, 1516, 1613
PspN4I	GGNNCC	1 cut(s)	60
PspPI	GGNCC	2 cut(s)	591, 1409
PstI	CTGCAG	1 cut(s)	361
PstNI	CAGNNNCTG	1 cut(s)	1052
PvuI	CGATCG	1 cut(s)	301
PvuII	CAGCTG	2 cut(s)	862, 1226
RsaI	GTAC	5 cut(s)	533, 722, 1240, 1483, 1778
RsaNI	GTAC	5 cut(s)	532, 721, 1239, 1482, 1777
RseI	CAYNNNNRTG	3 cut(s)	564, 632, 1875
SacI	GAGCTC	1 cut(s)	277
SapI	GCTCTTC	1 cut(s)	1068
SaqAI	TTAA	6 cut(s)	392, 618, 854, 873, 990, 1736
SatI	GCNGC	4 cut(s)	663, 860, 1299, 1719
Sau3AI	GATC	7 cut(s)	14, 265, 298, 568, 1017, 1663, 1697
Sau96I	GGNCC	2 cut(s)	591, 1409
ScaI	AGTACT	1 cut(s)	1240
SchI	GAGTC	3 cut(s)	21, 883, 1043
ScrFI	CCNGG	4 cut(s)	270, 1413, 1518, 1615
SduI	GDGCHC	4 cut(s)	277, 793, 1352, 1592
SfaNI	GCATC	3 cut(s)	970, 1033, 1072
SfcI	CTRYAG	1 cut(s)	357
Sfr274I	CTCGAG	1 cut(s)	1455
SinI	GGWCC	1 cut(s)	591
SlaI	CTCGAG	1 cut(s)	1455
SmiMI	CAYNNNNRTG	3 cut(s)	564, 632, 1875
SmlI	CTYRAG	4 cut(s)	100, 742, 1234, 1455
SmoI	CTYRAG	4 cut(s)	100, 742, 1234, 1455
SphI	GCATGC	2 cut(s)	1469, 1889
Sse9I	AATT	4 cut(s)	638, 850, 1737, 1812
SsiI	CCGC	2 cut(s)	20, 704
SspMI	CTAG	1 cut(s)	35
SstI	GAGCTC	1 cut(s)	277
StyD4I	CCNGG	4 cut(s)	268, 1411, 1516, 1613
StyI	CCWWGG	1 cut(s)	899
TaaI	ACNGT	8 cut(s)	143, 599, 725, 753, 974, 1535, 1794, 1833
TaiI	ACGT	2 cut(s)	113, 287
TaqI	TCGA	8 cut(s)	68, 297, 892, 1020, 1147, 1456, 1557, 1786
TasI	AATT	4 cut(s)	638, 850, 1737, 1812
TatI	WGTACW	3 cut(s)	1238, 1481, 1776
TfiI	GAWTC	3 cut(s)	214, 548, 1805
Tru1I	TTAA	6 cut(s)	392, 618, 854, 873, 990, 1736
Tru9I	TTAA	6 cut(s)	392, 618, 854, 873, 990, 1736
TscAI	CASTG	6 cut(s)	148, 758, 770, 823, 1137, 1592
TseFI	GTSAC	5 cut(s)	106, 111, 623, 919, 1529
TseI	GCWGC	4 cut(s)	662, 859, 1298, 1718
Tsp45I	GTSAC	5 cut(s)	106, 111, 623, 919, 1529
TspDTI	ATGAA	5 cut(s)	597, 621, 962, 1464, 1575
TspRI	CASTG	6 cut(s)	148, 758, 770, 823, 1137, 1592
VpaK11BI	GGWCC	1 cut(s)	591
XapI	RAATTY	2 cut(s)	638, 850
XceI	RCATGY	4 cut(s)	187, 631, 1469, 1889
XhoI	CTCGAG	1 cut(s)	1455
XmnI	GAANNNNTTC	1 cut(s)	552
XspI	CTAG	1 cut(s)	35
ZraI	GACGTC	1 cut(s)	111
ZrmI	AGTACT	1 cut(s)	1240
Zsp2I	ATGCAT	1 cut(s)	1471

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.