RLG00000016342

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020430
Physical Location & Seq
Reverse (-)
5774232 .. 5774700
469 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016342

Sequence Viewer

Length: 390 bp
ATGAAGTACACAGAAGCTGTAAATAAGAAAAGATCTCGTAGTTGCAACCAAGACCTCAATCTCGCAGACTACTTGAATCCCAAGAAAAAGATGAAGCTTGACAGTGGGAAAAATTGTGGTGAGGCACTAGATAAGTTATCCGGAGAGGCCTTGAAGGATTTATCTAGTTTCGTGAACTCCACGCTACACAAATTGGATCAGAAACTTGAGGGATATAAATCTGAACTTATCGATCAGCCTTACTACATCAGTAATTGGAACCCTCTAGAGCTCAATGAAAATTCATCGGACACTGAAATGTTTAAAACCAATCTACTATCGTACCAGGGCAAGCTTTTCAGTACCCTAATTTTCAAAGTGCCCAGTGATTTTGGAAACGTGGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.71

Weight (kDa)

6.58

Isoelectric Point (pI)

27.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020430)

Species Orthologous Gene IDs
pyrus_communis pycom02g05810
rosa_laevigata RLG00000016342
rosa_multiflora Rmu_sc0014880.1_g000001
rosa_roxburghii Rroxscaffold_2G00148740
rosa_samantha Rh2AG075700 Rh2DG074400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 140
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 280
AfaI GTAC 3 cut(s) 8, 323, 343
AgsI TTSAA 3 cut(s) 76, 154, 355
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 4 cut(s) 17, 97, 271, 334
AluI AGCT 4 cut(s) 17, 97, 271, 334
Alw21I GWGCWC 1 cut(s) 273
AlwI GGATC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 17
Aor13HI TCCGGA 1 cut(s) 140
AoxI GGCC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 363
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 326
BfaI CTAG 3 cut(s) 128, 165, 266
BglII AGATCT 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 357
BmuI ACTGGG 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 325
BsaWI WCCGGW 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 217
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 1 cut(s) 326
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 325
BseJI GATNNNNATC 1 cut(s) 217
BseNI ACTGG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 363
BshFI GGCC 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 141
BsnI GGCC 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 273, 363
Bsp13I TCCGGA 1 cut(s) 140
Bsp143I GATC 3 cut(s) 32, 196, 232
BspANI GGCC 1 cut(s) 149
BspDI ATCGAT 1 cut(s) 231
BspEI TCCGGA 1 cut(s) 140
BspLI GGNNCC 1 cut(s) 260
BspPI GGATC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 3 cut(s) 32, 196, 232
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 35, 199, 235
BstMBI GATC 3 cut(s) 32, 196, 232
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 231
BsuRI GGCC 1 cut(s) 149
BsuTUI ATCGAT 1 cut(s) 231
BtsIMutI CAGTG 3 cut(s) 109, 291, 370
Cac8I GCNNGC 1 cut(s) 332
CaiI CAGNNNCTG 1 cut(s) 17
ClaI ATCGAT 1 cut(s) 231
Csp6I GTAC 3 cut(s) 7, 322, 342
CviJI RGCY 6 cut(s) 17, 97, 149, 238, 271, 334
CviKI_1 RGCY 6 cut(s) 17, 97, 149, 238, 271, 334
CviQI GTAC 3 cut(s) 7, 322, 342
DpnI GATC 3 cut(s) 34, 198, 234
DpnII GATC 3 cut(s) 32, 196, 232
DraI TTTAAA 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 273
FaiI YATR 1 cut(s) 216
FriOI GRGCYC 1 cut(s) 273
FspBI CTAG 3 cut(s) 128, 165, 266
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 95, 332
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 9, 175, 382
Hpy188I TCNGA 3 cut(s) 201, 223, 289
Hpy188III TCNNGA 3 cut(s) 141, 172, 266
Hpy8I GTNNAC 3 cut(s) 9, 175, 382
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 378
Kpn2I TCCGGA 1 cut(s) 140
Kzo9I GATC 3 cut(s) 32, 196, 232
LpnPI CCDG 4 cut(s) 154, 311, 338, 376
MaeI CTAG 3 cut(s) 128, 165, 266
MaeII ACGT 1 cut(s) 378
MalI GATC 3 cut(s) 34, 198, 234
MboI GATC 3 cut(s) 32, 196, 232
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 273, 363
MluCI AATT 6 cut(s) 112, 191, 253, 280, 348, 385
MnlI CCTC 5 cut(s) 65, 115, 139, 202, 273
MroI TCCGGA 1 cut(s) 140
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
NdeII GATC 3 cut(s) 32, 196, 232
NlaIV GGNNCC 1 cut(s) 260
PceI AGGCCT 1 cut(s) 149
PfeI GAWTC 1 cut(s) 76
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 260
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 3 cut(s) 8, 323, 343
RsaNI GTAC 3 cut(s) 7, 322, 342
RseI CAYNNNNRTG 1 cut(s) 296
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 303
Sau3AI GATC 3 cut(s) 32, 196, 232
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 273, 363
SetI ASST 6 cut(s) 19, 57, 99, 273, 336, 381
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 6 cut(s) 112, 191, 253, 280, 348, 385
SseBI AGGCCT 1 cut(s) 149
SspMI CTAG 3 cut(s) 128, 165, 266
SstI GAGCTC 1 cut(s) 273
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 231
TasI AATT 6 cut(s) 112, 191, 253, 280, 348, 385
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 109, 298, 370
TspDTI ATGAA 4 cut(s) 17, 107, 273, 291
TspRI CASTG 3 cut(s) 109, 298, 370
XapI RAATTY 1 cut(s) 280
XbaI TCTAGA 1 cut(s) 265
XspI CTAG 3 cut(s) 128, 165, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.