Rroxscaffold_2G00148740
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0020430
Physical Location & Seq
Forward (+)
86354912 .. 86357429
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148740.1

Sequence Viewer

Length: 942 bp
ATGGTGCTGAAGGGAGAGTGGAAGGCGAAAGACCTATCCGCGCAATGCAAATCGCAGATCTGCATTGTTAGCTACAACGGTTCCTACGACAAGGGTATCCAGGTCTGGCCTGAAGACCAAACTGCGGCCAGAGACATCATCATAAAGTACACAGAAGCTGTAAATAAGAAAAGATCTCGTAGTTGCAACCAAGACCTCAATCTCGCAGACTACTTGAATCCTAAGAAAAAGATGAAGCTTGACAGTGGGAAAAATTGTGGTGAGGCACTAGATAAGTTATCTGGAGAGGCCTTGAAGGATTTATCTAGTTTCGTGAACTCCATGCTACACAAATTGGATCAGAAACTTGAGGGATATAAATCTGAACTTATCGATCAGCCTTACTACATCAGTAATTGGAACCCTCTTGAGCTCAATGAAAATTCCTCGGACACTGAAATGTTTAAAGGCTTTAATGACGGTCAATCTACTACGGTACCCGGGCAAGCTTTTCAGTACCCTAATTTTCAAAGTGGTACTGGGAGTCTGGGACTAATAGTACGATTGATGATCATCATAGCATTAATGTTCATCATCCATGGGGGATTTTGCAGACCAGTGATTTTGGAAACGTGGACAAGTTCGGTTCAATGGGGGAGAAGTGGGAGAACTACAGTATTAACAAAACTTTTGTCAACAACATTTTTGCTTCCATTGATTTTAAGAATTGCTCCAACGATGTTGCTTCTTCTGTTCTGCAAGGACATCTGGTTCTTCTACTCCATGTACTGGCATGGTCGATCAGGGACATCGCCAGTCCACTCATTTTTAGGGTTTGAAGCTGTGGAGCCAAACAACTTTTCTCTGAAGGATATTACACTGCCTTGTAATGAACAAAACAATATGGATCTTCTTCTTTATCAGAGTCATCATTATGATGGTTCCTCAATCGTCCCCTGTTAA

Protein Analysis

313

Amino Acids

35.4

Weight (kDa)

6.98

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020430)

Species Orthologous Gene IDs
pyrus_communis pycom02g05810
rosa_laevigata RLG00000016342
rosa_multiflora Rmu_sc0014880.1_g000001
rosa_roxburghii Rroxscaffold_2G00148740
rosa_samantha Rh2AG075700 Rh2DG074400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 768
AccII CGCG 1 cut(s) 41
AciI CCGC 2 cut(s) 39, 125
AclWI GGATC 2 cut(s) 345, 894
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 3 cut(s) 29, 132, 866
AfaI GTAC 6 cut(s) 149, 477, 497, 517, 540, 767
AfiI CCNNNNNNNGG 2 cut(s) 124, 768
AgsI TTSAA 5 cut(s) 217, 295, 509, 629, 818
AjnI CCWGG 1 cut(s) 99
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
AluBI AGCT 6 cut(s) 72, 158, 238, 412, 488, 821
AluI AGCT 6 cut(s) 72, 158, 238, 412, 488, 821
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 2 cut(s) 345, 894
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 3 cut(s) 107, 126, 288
ApoI RAATTY 1 cut(s) 421
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 1 cut(s) 563
Asp718I GGTACC 1 cut(s) 475
AspLEI GCGC 1 cut(s) 43
AsuC2I CCSGG 2 cut(s) 480, 481
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 479
BaeI ACNNNNGTAYC 4 cut(s) 79, 112, 459, 492
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 414
BccI CCATC 1 cut(s) 911
BciT130I CCWGG 1 cut(s) 101
BciVI GTATCC 1 cut(s) 107
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 2 cut(s) 480, 481
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 2 cut(s) 269, 306
BfmI CTRYAG 1 cut(s) 651
BfuI GTATCC 1 cut(s) 107
BglII AGATCT 2 cut(s) 57, 173
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 3 cut(s) 101, 480, 481
BmeT110I CYCGRG 1 cut(s) 479
BmiI GGNNCC 5 cut(s) 82, 401, 477, 828, 922
BmrFI CCNGG 3 cut(s) 101, 480, 481
BmrI ACTGGG 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 303
BpuEI CTTGAG 2 cut(s) 368, 428
BpuMI CCSGG 2 cut(s) 480, 481
Bsa29I ATCGAT 1 cut(s) 372
BsaBI GATNNNNATC 2 cut(s) 358, 551
BsaJI CCNNGG 3 cut(s) 426, 479, 577
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 768
Bse1I ACTGG 4 cut(s) 523, 596, 773, 794
Bse3DI GCAATG 1 cut(s) 50
Bse8I GATNNNNATC 2 cut(s) 358, 551
BseBI CCWGG 1 cut(s) 101
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 426, 479, 577
BseGI GGATG 1 cut(s) 573
BseJI GATNNNNATC 2 cut(s) 358, 551
BseLI CCNNNNNNNGG 2 cut(s) 124, 768
BseMI GCAATG 1 cut(s) 50
BseNI ACTGG 4 cut(s) 523, 596, 773, 794
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 3 cut(s) 109, 128, 290
BshNI GGYRCC 1 cut(s) 475
BshVI ATCGAT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 480
BslFI GGGAC 3 cut(s) 543, 799, 917
BslI CCNNNNNNNGG 2 cut(s) 124, 768
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 3 cut(s) 543, 799, 917
BsnI GGCC 3 cut(s) 109, 128, 290
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 2 cut(s) 39, 125
BspANI GGCC 3 cut(s) 109, 128, 290
BspDI ATCGAT 1 cut(s) 372
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 5 cut(s) 82, 401, 477, 828, 922
BspPI GGATC 2 cut(s) 345, 894
BspT107I GGYRCC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 50
BsrI ACTGG 4 cut(s) 523, 596, 773, 794
BssECI CCNNGG 3 cut(s) 426, 479, 577
BssMI GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
BssT1I CCWWGG 1 cut(s) 577
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 5 cut(s) 80, 245, 461, 475, 655
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 1 cut(s) 573
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 7 cut(s) 60, 176, 340, 376, 552, 782, 889
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 3 cut(s) 99, 478, 479
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 3 cut(s) 57, 173, 886
BstYI RGATCY 3 cut(s) 57, 173, 886
Bsu15I ATCGAT 1 cut(s) 372
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 3 cut(s) 109, 128, 290
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 774
BtsCI GGATG 1 cut(s) 573
BtsI GCAGTG 1 cut(s) 857
BtsIMutI CAGTG 4 cut(s) 250, 432, 603, 857
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 43
Cfr9I CCCGGG 1 cut(s) 479
ClaI ATCGAT 1 cut(s) 372
Csp6I GTAC 6 cut(s) 148, 476, 496, 516, 539, 766
CviAII CATG 4 cut(s) 322, 578, 763, 773
CviQI GTAC 6 cut(s) 148, 476, 496, 516, 539, 766
DdeI CTNAG 1 cut(s) 222
DpnI GATC 7 cut(s) 59, 175, 339, 375, 551, 781, 888
DpnII GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
DraI TTTAAA 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 577
Eco147I AGGCCT 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 29, 132, 866
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 577
FaeI CATG 4 cut(s) 325, 581, 766, 776
FaiI YATR 9 cut(s) 143, 323, 357, 557, 579, 764, 774, 884, 915
FaqI GGGAC 3 cut(s) 543, 799, 917
FatI CATG 4 cut(s) 321, 577, 762, 772
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 560
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 2 cut(s) 269, 306
GlaI GCGC 1 cut(s) 42
GluI GCNGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 3 cut(s) 109, 128, 290
HapII CCGG 1 cut(s) 480
HhaI GCGC 1 cut(s) 43
Hin1II CATG 4 cut(s) 325, 581, 766, 776
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HindIII AAGCTT 2 cut(s) 236, 486
HinfI GANTC 3 cut(s) 217, 523, 904
HpaII CCGG 1 cut(s) 480
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 5 cut(s) 150, 316, 615, 675, 799
Hpy188I TCNGA 5 cut(s) 342, 364, 430, 846, 903
Hpy188III TCNNGA 3 cut(s) 282, 313, 407
Hpy8I GTNNAC 5 cut(s) 150, 316, 615, 675, 799
HpyAV CCTTC 4 cut(s) 4, 16, 289, 841
HpyCH4III ACNGT 5 cut(s) 80, 245, 461, 475, 655
HpyCH4IV ACGT 1 cut(s) 611
HpyCH4V TGCA 5 cut(s) 48, 63, 186, 591, 738
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 611
Hsp92II CATG 4 cut(s) 325, 581, 766, 776
HspAI GCGC 1 cut(s) 41
KpnI GGTACC 1 cut(s) 479
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
LmnI GCTCC 2 cut(s) 715, 826
MaeI CTAG 2 cut(s) 269, 306
MaeII ACGT 1 cut(s) 611
MalI GATC 7 cut(s) 59, 175, 339, 375, 551, 781, 888
MboI GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
MboII GAAGA 5 cut(s) 125, 719, 745, 881, 884
MflI RGATCY 3 cut(s) 57, 173, 886
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 6 cut(s) 253, 332, 394, 421, 502, 705
MlyI GAGTC 2 cut(s) 532, 913
MmeI TCCRAC 1 cut(s) 737
MnlI CCTC 7 cut(s) 206, 256, 280, 343, 414, 436, 934
MseI TTAA 6 cut(s) 444, 453, 563, 659, 701, 940
MslI CAYNNNNRTG 3 cut(s) 437, 912, 915
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 3 cut(s) 101, 480, 481
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 69
NciI CCSGG 2 cut(s) 480, 481
NcoI CCATGG 1 cut(s) 577
NdeII GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
NlaIII CATG 4 cut(s) 325, 581, 766, 776
NlaIV GGNNCC 5 cut(s) 82, 401, 477, 828, 922
PceI AGGCCT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 768
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 2 cut(s) 531, 912
PpsI GAGTC 2 cut(s) 531, 912
PshBI ATTAAT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 5 cut(s) 82, 401, 477, 828, 922
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 3 cut(s) 57, 173, 886
RsaI GTAC 6 cut(s) 149, 477, 497, 517, 540, 767
RsaNI GTAC 6 cut(s) 148, 476, 496, 516, 539, 766
RseI CAYNNNNRTG 3 cut(s) 437, 912, 915
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 6 cut(s) 444, 453, 563, 659, 701, 940
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 7 cut(s) 57, 173, 337, 373, 549, 779, 886
SchI GAGTC 2 cut(s) 532, 913
ScrFI CCNGG 3 cut(s) 101, 480, 481
SduI GDGCHC 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 651
SmaI CCCGGG 1 cut(s) 481
SmiMI CAYNNNNRTG 3 cut(s) 437, 912, 915
SmlI CTYRAG 2 cut(s) 347, 407
SmoI CTYRAG 2 cut(s) 347, 407
Sse9I AATT 6 cut(s) 253, 332, 394, 421, 502, 705
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 2 cut(s) 39, 125
SspMI CTAG 2 cut(s) 269, 306
SstI GAGCTC 1 cut(s) 414
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 99, 478, 479
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 5 cut(s) 80, 245, 461, 475, 655
TaiI ACGT 1 cut(s) 614
TaqI TCGA 2 cut(s) 372, 778
TasI AATT 6 cut(s) 253, 332, 394, 421, 502, 705
TatI WGTACW 2 cut(s) 147, 765
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 6 cut(s) 444, 453, 563, 659, 701, 940
Tru9I TTAA 6 cut(s) 444, 453, 563, 659, 701, 940
TscAI CASTG 4 cut(s) 250, 439, 603, 864
TspDTI ATGAA 4 cut(s) 248, 432, 559, 885
TspMI CCCGGG 1 cut(s) 479
TspRI CASTG 4 cut(s) 250, 439, 603, 864
Van91I CCANNNNNTGG 1 cut(s) 768
VspI ATTAAT 1 cut(s) 563
XapI RAATTY 1 cut(s) 421
XmaI CCCGGG 1 cut(s) 479
XspI CTAG 2 cut(s) 269, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.