Rh2AG075700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020430
Physical Location & Seq
Reverse (-)
6347878 .. 6348876
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG075700.1

Sequence Viewer

Length: 537 bp
ATGAAGCTTGACAGTGGGAAAAATTGTGGTGAGGCACTAGATAATAAGTTATCCGGAGAGGCCTTGAAGGATTTATCTAGTTTCGTGAACTCCACGCTACACAAATTGTATCAGAAACTTGAGGGATATAAATCTGAAGTTATCGATCAGCCTTACTACATCAGTAATTGGAACCCTCTTGAGCTCAATGAAAATTCCTCGGACACTGAAATGTTTAAAGGTTTTAATGACGGTCAATCTACTATGGTCCCAGGGCAAGCTTTTCAGTACCCTAATTTTCAAAGTGGTACTGGGAGTCTGGGACTAATAGTACGATTGATGATCATCATAGTATTAATGTTCATCATCCATGGGGGATTTTGCAGCCCAGTGATTTTGGAAACGTGGACAAGTTCGGTTCAATGGGGGAGAAGTCGAAGAACTACACCCAACACATTGGGCCTGCCCACATTCCAAAGCCTTCGGGCTCAAGAAAGGCGAAACAAAACCCCCCAGAATTTTGAAGAAAATTCCGAATTTGCCACTGCGGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.01

Weight (kDa)

6.13

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020430)

Species Orthologous Gene IDs
pyrus_communis pycom02g05810
rosa_laevigata RLG00000016342
rosa_multiflora Rmu_sc0014880.1_g000001
rosa_roxburghii Rroxscaffold_2G00148740
rosa_samantha Rh2AG075700 Rh2DG074400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 1 cut(s) 527
AcsI RAATTY 4 cut(s) 193, 496, 508, 515
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 3 cut(s) 269, 289, 312
AgsI TTSAA 4 cut(s) 67, 281, 401, 503
AjnI CCWGG 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 3 cut(s) 7, 184, 260
AluI AGCT 3 cut(s) 7, 184, 260
Alw21I GWGCWC 1 cut(s) 186
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 2 cut(s) 60, 439
ApeKI GCWGC 1 cut(s) 363
ApoI RAATTY 4 cut(s) 193, 496, 508, 515
AseI ATTAAT 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 247, 439
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 247
BanII GRGCYC 2 cut(s) 186, 469
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 375
BciT130I CCWGG 1 cut(s) 252
BclI TGATCA 1 cut(s) 321
BfaI CTAG 2 cut(s) 38, 78
BisI GCNGC 1 cut(s) 364
BlsI GCNGC 1 cut(s) 365
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 247, 439
BmiI GGNNCC 2 cut(s) 173, 249
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 2 cut(s) 300, 362
BmuI ACTGGG 2 cut(s) 300, 362
BpuEI CTTGAG 3 cut(s) 140, 200, 453
Bsa29I ATCGAT 1 cut(s) 144
BsaBI GATNNNNATC 2 cut(s) 130, 323
BsaJI CCNNGG 4 cut(s) 198, 250, 251, 349
BsaWI WCCGGW 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 295, 368
Bse8I GATNNNNATC 2 cut(s) 130, 323
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 198, 250, 251, 349
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 2 cut(s) 130, 323
BseNI ACTGG 2 cut(s) 295, 368
BseXI GCAGC 1 cut(s) 375
BshFI GGCC 2 cut(s) 62, 441
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 2 cut(s) 233, 315
BsmFI GGGAC 2 cut(s) 233, 315
BsnI GGCC 2 cut(s) 62, 441
Bsp1286I GDGCHC 2 cut(s) 186, 469
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 2 cut(s) 145, 321
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 1 cut(s) 527
BspANI GGCC 2 cut(s) 62, 441
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 173, 249
BsrI ACTGG 2 cut(s) 295, 368
BssECI CCNNGG 4 cut(s) 198, 250, 251, 349
BssMI GATC 2 cut(s) 145, 321
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 14, 233
BstC8I GCNNGC 2 cut(s) 258, 443
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 1 cut(s) 345
BstKTI GATC 2 cut(s) 148, 324
BstMBI GATC 2 cut(s) 145, 321
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 375
BstXI CCANNNNNNTGG 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 2 cut(s) 62, 441
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 349
BtsCI GGATG 1 cut(s) 345
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 4 cut(s) 19, 204, 375, 522
Cac8I GCNNGC 2 cut(s) 258, 443
Cfr13I GGNCC 2 cut(s) 247, 439
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 3 cut(s) 268, 288, 311
CviAII CATG 1 cut(s) 350
CviJI RGCY 9 cut(s) 7, 62, 151, 184, 260, 366, 441, 459, 467
CviKI_1 RGCY 9 cut(s) 7, 62, 151, 184, 260, 366, 441, 459, 467
CviQI GTAC 3 cut(s) 268, 288, 311
DpnI GATC 2 cut(s) 147, 323
DpnII GATC 2 cut(s) 145, 321
DraI TTTAAA 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 186, 469
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 2 cut(s) 186, 469
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 1 cut(s) 353
FaiI YATR 4 cut(s) 129, 245, 329, 351
FaqI GGGAC 2 cut(s) 233, 315
FatI CATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 364
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 2 cut(s) 186, 469
Fsp4HI GCNGC 1 cut(s) 364
FspBI CTAG 2 cut(s) 38, 78
GluI GCNGC 1 cut(s) 364
HaeIII GGCC 2 cut(s) 62, 441
HapII CCGG 1 cut(s) 54
Hin1II CATG 1 cut(s) 353
HindIII AAGCTT 2 cut(s) 5, 258
HinfI GANTC 1 cut(s) 295
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 88, 387
Hpy188I TCNGA 4 cut(s) 114, 136, 202, 514
Hpy188III TCNNGA 4 cut(s) 54, 85, 179, 470
Hpy8I GTNNAC 2 cut(s) 88, 387
HpyAV CCTTC 2 cut(s) 61, 470
HpyCH4III ACNGT 2 cut(s) 14, 233
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 383
Hsp92II CATG 1 cut(s) 353
Kpn2I TCCGGA 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 321
Kzo9I GATC 2 cut(s) 145, 321
LpnPI CCDG 8 cut(s) 67, 237, 264, 276, 284, 381, 455, 506
Lsp1109I GCAGC 1 cut(s) 375
MaeI CTAG 2 cut(s) 38, 78
MaeII ACGT 1 cut(s) 383
MalI GATC 2 cut(s) 147, 323
MboI GATC 2 cut(s) 145, 321
MboII GAAGA 2 cut(s) 429, 515
MhlI GDGCHC 2 cut(s) 186, 469
MluCI AATT 8 cut(s) 22, 104, 166, 193, 274, 496, 508, 515
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 5 cut(s) 25, 52, 115, 186, 208
MroI TCCGGA 1 cut(s) 53
MseI TTAA 3 cut(s) 216, 225, 335
MslI CAYNNNNRTG 2 cut(s) 209, 527
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
NcoI CCATGG 1 cut(s) 349
NdeII GATC 2 cut(s) 145, 321
NlaIII CATG 1 cut(s) 353
NlaIV GGNNCC 2 cut(s) 173, 249
OliI CACNNNNGTG 1 cut(s) 527
PasI CCCWGGG 1 cut(s) 251
PceI AGGCCT 1 cut(s) 62
PkrI GCNGC 1 cut(s) 365
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 173, 249
PspPI GGNCC 2 cut(s) 247, 439
RsaI GTAC 3 cut(s) 269, 289, 312
RsaNI GTAC 3 cut(s) 268, 288, 311
RseI CAYNNNNRTG 2 cut(s) 209, 527
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 3 cut(s) 216, 225, 335
SatI GCNGC 1 cut(s) 364
Sau3AI GATC 2 cut(s) 145, 321
Sau96I GGNCC 2 cut(s) 247, 439
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 2 cut(s) 186, 469
SetI ASST 5 cut(s) 9, 186, 223, 262, 386
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 209, 527
SmlI CTYRAG 3 cut(s) 119, 179, 468
SmoI CTYRAG 3 cut(s) 119, 179, 468
Sse9I AATT 8 cut(s) 22, 104, 166, 193, 274, 496, 508, 515
SseBI AGGCCT 1 cut(s) 62
SsiI CCGC 1 cut(s) 527
SspMI CTAG 2 cut(s) 38, 78
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 2 cut(s) 14, 233
TaiI ACGT 1 cut(s) 386
TaqI TCGA 2 cut(s) 144, 415
TasI AATT 8 cut(s) 22, 104, 166, 193, 274, 496, 508, 515
Tru1I TTAA 3 cut(s) 216, 225, 335
Tru9I TTAA 3 cut(s) 216, 225, 335
TscAI CASTG 4 cut(s) 19, 211, 375, 529
TseI GCWGC 1 cut(s) 363
TspDTI ATGAA 3 cut(s) 17, 204, 331
TspRI CASTG 4 cut(s) 19, 211, 375, 529
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 335
XapI RAATTY 4 cut(s) 193, 496, 508, 515
XspI CTAG 2 cut(s) 38, 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.