RLG00000017740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018777
Physical Location & Seq
Reverse (-)
20635341 .. 20637967
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017740

Sequence Viewer

Length: 1644 bp
ATGGAGAAGAAGAGGAAGAAGGATGAGAAAGTTGCTTGCACCACTAGTCAAGAACCAGAGACAGACCACCATATCTTGAGCAAGGATCACCAAAGCCAAGACGATGCTGAACCTCTAACTAAGAGTACAAACACTGATAGTCCTTCGAATGGCAATGTACCTTCCCTATGTCACCAATACCCAGTAACTTTTCCATGGCCGTACACGCCTCAACATGGTGGGGAGCAATGTCCTTTGGTGTCTAGACCCTTTGTTGCAACTCAATCACCGCTGCCTGGCATTATAAGCCAATGGCCGCAGTTTCCACATCAGGTCCAGCATGGCCAGCCACCACAGAACTATTCCCAGTCAGCTGCTCCCTTTTGGCTACCACAGCAGCCTGGTTATCCATTTTCTGCCTCCAATGCACCGCCAACTTTCCAGCCATTTGTTGCTGTTGGAGCAACTGATATTAGTGGTGGAACTTTAACAACTAACCAAACTCAGGTTCCCAATTTCTATTACCATGTTGGGTACCCATATCCAGGCTTTCCAGGTTCATGTGATTCCTTATCTTGGTGGAATCAAGCACAAGCACCACAACCTCTGTGCACATATGCTTTTCCAGGAGGCTGCATCTCTTCACCACCTGCATCGCTGCCTAGTTGTTCTGCAACTCTTGGGCAAACCTTTGAAAAAGGAAGTATCAGGCCACCAGCAAAACTTTCTAAGAAGCACCAGCAGCTTTGGGATGTTCAATCTGCAGAAAATGTTCAGCTATGGAGTGCAATCAACCACTTGCAATCAGAAGTAACTGATTACAAAAATTGCTTCACAAGGCTCGAAGCAGAACTGTCTTCTCTTAAAGCAGTAGCAGAAGAGACCACTGCTCAAGTTAGTGGGGCTGTTTTATCTGCAAAACCATCAAAGAGAGGGAGACCAAAGAGATCAGTTGGTTCAGCTGATGCAGTGCATTCTCCTGATAAGTCTCATCACCGAGCTCGTGGCAGAAAATCTGTAGTACGCAATGCTCAACAGTTTGATAGTAAATCACATTTGTTTGAGAAAGTAATCCTCAACAAGGTGGAAGACAAAGAAAAAGCATGTCACTTAACAGCCGCTGTTGAGCAAGGAAACAATGCCTCACATGTTGTCACACATTCTGGTGTCAATATGGAGATAAATGGAACCAGTTCGACAATGCCTGCATTCCACTATCAATTTCAGCACGAACTTCCTGCTGTCCAAACATGTGGAAATGGGAGTTCTGCTTCTTCAGAAATGAAAGGTACTGATGTCAAGGCTAACAACTGTAGGACTACCTACCCTATCTATCCTCAGAACATCCCATGGCCTTATAACAATACCTCTGAAATGGAGAGAAACGAGCTAAATATTGGCCCCAATGGTTTGTACAATAGCGGTATTGTTATCACACAAGGGGGACAGATCATCCCTGGATGGAGTTTTGGAAATGAAGAGGATGCTTCAGAAAAGCAGGAAGATGCCATGTTAGGATCAGGAAAGAATGACAATAAGGAGACCATGGGAGATGAATCCAGTTCAGGAGCAGAAAAAGTTGCTCGAACAAACAATGAGGGTGGCTTCAAAATGGATGATACAGAAGGAACTAGCAATAAGTGTCTTGCTCCACCCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

548

Amino Acids

59.61

Weight (kDa)

6.17

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18650
prunus_persica Prupe.6G212800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109471
rosa_laevigata RLG00000017740
rosa_multiflora Rmu_sc0000332.1_g000028
rosa_roxburghii Rroxscaffold_2G00134210
rosa_rugosa Rorug02G0160400
rosa_samantha Rh2BG222700 Rh2CG214300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 284, 1338
AarI CACCTGC 1 cut(s) 637
Acc36I ACCTGC 1 cut(s) 637
Acc65I GGTACC 1 cut(s) 513
AccB1I GGYRCC 1 cut(s) 513
AciI CCGC 5 cut(s) 269, 296, 410, 1098, 1401
AclWI GGATC 2 cut(s) 93, 1504
AcoI YGGCCR 3 cut(s) 197, 293, 322
AcuI CTGAAG 2 cut(s) 1239, 1452
AfaI GTAC 7 cut(s) 127, 159, 203, 515, 1002, 1270, 1394
AfiI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
AflIII ACRYGT 2 cut(s) 1126, 1229
AgsI TTSAA 3 cut(s) 674, 737, 1588
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 6 cut(s) 353, 724, 757, 941, 980, 1369
AluI AGCT 6 cut(s) 353, 724, 757, 941, 980, 1369
Alw21I GWGCWC 2 cut(s) 593, 982
Alw26I GTCTC 5 cut(s) 53, 854, 910, 972, 1514
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 2 cut(s) 93, 1504
AlwNI CAGNNNCTG 1 cut(s) 1100
AoxI GGCC 6 cut(s) 197, 293, 322, 689, 1331, 1378
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 6 cut(s) 271, 353, 376, 612, 637, 721
Asp700I GAANNNNTTC 2 cut(s) 750, 1171
Asp718I GGTACC 1 cut(s) 513
AspS9I GGNCC 2 cut(s) 313, 1379
AsuHPI GGTGA 5 cut(s) 80, 164, 258, 615, 965
AsuII TTCGAA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 313
BaeGI GKGCMC 1 cut(s) 593
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BalI TGGCCA 1 cut(s) 324
BanI GGYRCC 1 cut(s) 513
BanII GRGCYC 1 cut(s) 982
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 2 cut(s) 828, 1074
Bbv12I GWGCWC 2 cut(s) 593, 982
BbvI GCAGC 6 cut(s) 258, 340, 388, 599, 624, 733
BccI CCATC 2 cut(s) 910, 1434
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BcoDI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
BfmI CTRYAG 3 cut(s) 741, 996, 1291
BfuAI ACCTGC 1 cut(s) 637
BisI GCNGC 8 cut(s) 272, 296, 354, 377, 613, 638, 722, 1098
BlsI GCNGC 8 cut(s) 273, 297, 355, 378, 614, 639, 723, 1099
Bme1390I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 313, 1379
BmiI GGNNCC 4 cut(s) 489, 515, 1168, 1381
BmrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BmrI ACTGGG 2 cut(s) 176, 340
BmsI GCATC 6 cut(s) 94, 624, 641, 934, 1453, 1474
BmuI ACTGGG 2 cut(s) 176, 340
BpiI GAAGAC 2 cut(s) 828, 1074
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 2 cut(s) 97, 855
BsaI GGTCTC 3 cut(s) 854, 910, 1514
BsaJI CCNNGG 4 cut(s) 194, 1328, 1435, 1524
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
Bse1I ACTGG 4 cut(s) 182, 346, 1170, 1539
Bse3DI GCAATG 3 cut(s) 160, 233, 1012
BseBI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BseDI CCNNGG 4 cut(s) 194, 1328, 1435, 1524
BseGI GGATG 7 cut(s) 28, 736, 1323, 1431, 1445, 1468, 1600
BseLI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BseMI GCAATG 3 cut(s) 160, 233, 1012
BseMII CTCAG 2 cut(s) 497, 1331
BseNI ACTGG 4 cut(s) 182, 346, 1170, 1539
BseSI GKGCMC 1 cut(s) 593
BseXI GCAGC 6 cut(s) 258, 340, 388, 599, 624, 733
BshFI GGCC 6 cut(s) 199, 295, 324, 691, 1333, 1380
BshNI GGYRCC 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 593, 982
BslFI GGGAC 1 cut(s) 1437
BslI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BsmAI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BsmFI GGGAC 1 cut(s) 1437
BsmI GAATGC 2 cut(s) 952, 1187
BsnI GGCC 6 cut(s) 199, 295, 324, 691, 1333, 1380
Bso31I GGTCTC 3 cut(s) 854, 910, 1514
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 593, 982
Bsp1407I TGTACA 1 cut(s) 1392
Bsp143I GATC 4 cut(s) 85, 926, 1428, 1496
Bsp19I CCATGG 3 cut(s) 194, 1328, 1524
BspACI CCGC 5 cut(s) 269, 296, 410, 1098, 1401
BspANI GGCC 6 cut(s) 199, 295, 324, 691, 1333, 1380
BspCNI CTCAG 2 cut(s) 496, 1330
BspLI GGNNCC 4 cut(s) 489, 515, 1168, 1381
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 93, 1504
BspT104I TTCGAA 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 513
BspTNI GGTCTC 3 cut(s) 854, 910, 1514
BsrDI GCAATG 3 cut(s) 160, 233, 1012
BsrGI TGTACA 1 cut(s) 1392
BsrI ACTGG 4 cut(s) 182, 346, 1170, 1539
BssECI CCNNGG 4 cut(s) 194, 1328, 1435, 1524
BssMI GATC 4 cut(s) 85, 926, 1428, 1496
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 3 cut(s) 194, 1328, 1524
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Bst4CI ACNGT 3 cut(s) 834, 1017, 1292
Bst6I CTCTTC 4 cut(s) 5, 625, 852, 1452
BstAUI TGTACA 1 cut(s) 1392
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 37, 326, 1185
BstDEI CTNAG 4 cut(s) 120, 483, 708, 1317
BstDSI CCRYGG 3 cut(s) 194, 1328, 1524
BstENI CCTNNNNNAGG 1 cut(s) 1058
BstF5I GGATG 7 cut(s) 28, 736, 1323, 1431, 1445, 1468, 1600
BstKTI GATC 4 cut(s) 88, 929, 1431, 1499
BstMAI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BstMBI GATC 4 cut(s) 85, 926, 1428, 1496
BstMWI GCNNNNNNNGC 7 cut(s) 205, 285, 325, 373, 404, 440, 721
BstNI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BstNSI RCATGY 3 cut(s) 1086, 1130, 1233
BstSCI CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1435
BstSFI CTRYAG 3 cut(s) 741, 996, 1291
BstSLI GKGCMC 1 cut(s) 593
BstV1I GCAGC 6 cut(s) 258, 340, 388, 599, 624, 733
BstV2I GAAGAC 2 cut(s) 828, 1074
BstXI CCANNNNNNTGG 1 cut(s) 1232
BsuRI GGCC 6 cut(s) 199, 295, 324, 691, 1333, 1380
BtgI CCRYGG 3 cut(s) 194, 1328, 1524
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 7 cut(s) 28, 736, 1323, 1431, 1445, 1468, 1600
BtsI GCAGTG 2 cut(s) 864, 954
BtsIMutI CAGTG 3 cut(s) 132, 864, 954
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 3 cut(s) 37, 326, 1185
CaiI CAGNNNCTG 1 cut(s) 1100
Cfr13I GGNCC 2 cut(s) 313, 1379
Csp6I GTAC 7 cut(s) 126, 158, 202, 514, 1001, 1269, 1393
CspCI CAANNNNNGTGG 2 cut(s) 1561, 1596
CviQI GTAC 7 cut(s) 126, 158, 202, 514, 1001, 1269, 1393
DdeI CTNAG 4 cut(s) 120, 483, 708, 1317
DpnI GATC 4 cut(s) 87, 928, 1430, 1498
DpnII GATC 4 cut(s) 85, 926, 1428, 1496
EaeI YGGCCR 3 cut(s) 197, 293, 322
Eam1104I CTCTTC 4 cut(s) 5, 625, 852, 1452
EarI CTCTTC 4 cut(s) 5, 625, 852, 1452
Ecl136II GAGCTC 1 cut(s) 980
Eco130I CCWWGG 3 cut(s) 194, 1328, 1524
Eco24I GRGCYC 1 cut(s) 982
Eco31I GGTCTC 3 cut(s) 854, 910, 1514
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 980
Eco57I CTGAAG 2 cut(s) 1239, 1452
EcoICRI GAGCTC 1 cut(s) 980
EcoNI CCTNNNNNAGG 1 cut(s) 1058
EcoRII CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
EcoT14I CCWWGG 3 cut(s) 194, 1328, 1524
EcoT38I GRGCYC 1 cut(s) 982
ErhI CCWWGG 3 cut(s) 194, 1328, 1524
FaqI GGGAC 1 cut(s) 1437
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 8 cut(s) 272, 296, 354, 377, 613, 638, 722, 1098
FokI GGATG 7 cut(s) 35, 743, 1310, 1418, 1452, 1475, 1607
FriOI GRGCYC 1 cut(s) 982
Fsp4HI GCNGC 8 cut(s) 272, 296, 354, 377, 613, 638, 722, 1098
FspBI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
GluI GCNGC 8 cut(s) 272, 296, 354, 377, 613, 638, 722, 1098
HaeIII GGCC 6 cut(s) 199, 295, 324, 691, 1333, 1380
HinfI GANTC 3 cut(s) 545, 562, 1535
HphI GGTGA 5 cut(s) 80, 164, 258, 615, 965
Hpy166II GTNNAC 2 cut(s) 204, 591
Hpy188I TCNGA 5 cut(s) 787, 1258, 1320, 1351, 1471
Hpy188III TCNNGA 6 cut(s) 50, 76, 243, 959, 1500, 1545
Hpy8I GTNNAC 2 cut(s) 204, 591
HpyAV CCTTC 4 cut(s) 13, 153, 171, 1598
HpyCH4III ACNGT 3 cut(s) 834, 1017, 1292
HpyF10VI GCNNNNNNNGC 7 cut(s) 205, 285, 325, 373, 404, 440, 721
HpyF3I CTNAG 4 cut(s) 120, 483, 708, 1317
KpnI GGTACC 1 cut(s) 517
Kzo9I GATC 4 cut(s) 85, 926, 1428, 1496
LmnI GCTCC 5 cut(s) 223, 361, 440, 1547, 1633
Lsp1109I GCAGC 6 cut(s) 258, 340, 388, 599, 624, 733
LweI GCATC 6 cut(s) 94, 624, 641, 934, 1453, 1474
MaeI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
MaeIII GTNAC 5 cut(s) 170, 184, 790, 1085, 1132
MalI GATC 4 cut(s) 87, 928, 1430, 1498
MboI GATC 4 cut(s) 85, 926, 1428, 1496
MhlI GDGCHC 2 cut(s) 593, 982
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 3 cut(s) 493, 805, 1199
MluNI TGGCCA 1 cut(s) 324
MmeI TCCRAC 1 cut(s) 418
Mox20I TGGCCA 1 cut(s) 324
MroXI GAANNNNTTC 2 cut(s) 750, 1171
MscI TGGCCA 1 cut(s) 324
MseI TTAA 3 cut(s) 467, 843, 1091
MslI CAYNNNNRTG 1 cut(s) 1143
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 4 cut(s) 271, 353, 941, 1100
MspR9I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Mva1269I GAATGC 2 cut(s) 952, 1187
MvaI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
MwoI GCNNNNNNNGC 7 cut(s) 205, 285, 325, 373, 404, 440, 721
NcoI CCATGG 3 cut(s) 194, 1328, 1524
NdeI CATATG 1 cut(s) 595
NdeII GATC 4 cut(s) 85, 926, 1428, 1496
NlaIV GGNNCC 4 cut(s) 489, 515, 1168, 1381
NmuCI GTSAC 3 cut(s) 170, 1085, 1132
NspI RCATGY 3 cut(s) 1086, 1130, 1233
NspV TTCGAA 1 cut(s) 146
PaqCI CACCTGC 1 cut(s) 637
PciI ACATGT 2 cut(s) 1126, 1229
PctI GAATGC 2 cut(s) 952, 1187
PdmI GAANNNNTTC 2 cut(s) 750, 1171
PfeI GAWTC 3 cut(s) 545, 562, 1535
PfoI TCCNGGA 1 cut(s) 604
PkrI GCNGC 8 cut(s) 273, 297, 355, 378, 614, 639, 723, 1099
PscI ACATGT 2 cut(s) 1126, 1229
PsiI TTATAA 2 cut(s) 284, 1338
Psp124BI GAGCTC 1 cut(s) 982
Psp6I CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
PspGI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
PspN4I GGNNCC 4 cut(s) 489, 515, 1168, 1381
PspPI GGNCC 2 cut(s) 313, 1379
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 1100
PvuII CAGCTG 2 cut(s) 353, 941
RsaI GTAC 7 cut(s) 127, 159, 203, 515, 1002, 1270, 1394
RsaNI GTAC 7 cut(s) 126, 158, 202, 514, 1001, 1269, 1393
RseI CAYNNNNRTG 1 cut(s) 1143
SacI GAGCTC 1 cut(s) 982
SaqAI TTAA 3 cut(s) 467, 843, 1091
SatI GCNGC 8 cut(s) 272, 296, 354, 377, 613, 638, 722, 1098
Sau3AI GATC 4 cut(s) 85, 926, 1428, 1496
Sau96I GGNCC 2 cut(s) 313, 1379
ScrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
SduI GDGCHC 2 cut(s) 593, 982
SfaNI GCATC 6 cut(s) 94, 624, 641, 934, 1453, 1474
SfcI CTRYAG 3 cut(s) 741, 996, 1291
SfuI TTCGAA 1 cut(s) 146
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 2 cut(s) 76, 870
SmoI CTYRAG 2 cut(s) 76, 870
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 3 cut(s) 493, 805, 1199
SsiI CCGC 5 cut(s) 269, 296, 410, 1098, 1401
SspI AATATT 1 cut(s) 1375
SspMI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
SstI GAGCTC 1 cut(s) 982
StyD4I CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1435
StyI CCWWGG 3 cut(s) 194, 1328, 1524
TaaI ACNGT 3 cut(s) 834, 1017, 1292
TaqI TCGA 4 cut(s) 146, 822, 1175, 1564
TasI AATT 3 cut(s) 493, 805, 1199
TatI WGTACW 2 cut(s) 125, 1392
TauI GCSGC 2 cut(s) 298, 1100
TfiI GAWTC 3 cut(s) 545, 562, 1535
Tru1I TTAA 3 cut(s) 467, 843, 1091
Tru9I TTAA 3 cut(s) 467, 843, 1091
TscAI CASTG 3 cut(s) 139, 871, 954
TseFI GTSAC 3 cut(s) 170, 1085, 1132
TseI GCWGC 6 cut(s) 271, 353, 376, 612, 637, 721
Tsp45I GTSAC 3 cut(s) 170, 1085, 1132
TspDTI ATGAA 4 cut(s) 528, 1277, 1470, 1548
TspRI CASTG 3 cut(s) 139, 871, 954
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 1058
XbaI TCTAGA 1 cut(s) 242
XceI RCATGY 3 cut(s) 1086, 1130, 1233
XmnI GAANNNNTTC 2 cut(s) 750, 1171
XspI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.