Rroxscaffold_2G00134210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018777
Physical Location & Seq
Forward (+)
71669430 .. 71672445
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134210.1

Sequence Viewer

Length: 1644 bp
ATGGAGAAGAAGAGGAAGAAGGATGAGAAAGTTGCTTGCACCACTAGTCAAGAACCAGAGACAGACCACCATATCTTGAGCAAGGATCACCAAAGCCAAGACAATGCTGAACCTCTAACAAAGAGTACAAACACTGATATTCCTTCGGATGGCAATGTACCTTCCCTATGTCACCAATACCCAGTAACTTTTCCATGGCCGTACATGCCTCAACATGGTGGGGAGCAATGCCCTTTGGTGTCTAGACCCTTTGTTGCAACTCAATCACCACCTCCTGGCATTATAAGCCAATGGCCGCAGTTTCCACATCAGGTCCAGCAAGGCCAGCCACCGCGGAACTATTCCCAGTCAGCTGCTCCCTTTTGGCTACCACAGCAGCCTGGTTATCCATTCTCTGCCTCCAATGCACCGTCAAATTTCCAGCCATTTGTTGCTGTTGGAGCAACTGATATTAGTGGTGGAACTTCAACAACAAACCAAAATCAGGTTCCCAATTTCTATTACCATGTTGGGTACCCATATCCAGGCTTTCCAGGTTCATGTGATTCCTTATCTTGGTGGAATCGAGCACAAGCACCACAACCTCTGTGCACATATGCTTTTCCAGGAGGCTGCATCTCTTCAGCACCTGCATCGCTGCCTAGTTGTTCTGCAACTCTTGCGCAAACCTTTGAAAAAGGAATTATCAGGGCACCAGCAGAACTTTCTAAGAAGCACCAGCAGCTTTGGGATGCTCAATCTGCAGAAAATGTTCAGCTATGGAGTGCAATCAACCACTTGCAATCAGAAGTAACTGATTACAAAAATTGCTTAACAAGGCTCGAAGCAGAACTGTCTTCTCTTAAAACAGTAGCAGAAGAGACCACTGCTCAAGTTAATGGGGCTGTTTTATCTGCAAAACCATCAAAGAGAGGGAGACCAAAGAGATCAGTTGCTTCAGCTGATGCAGTGCATTCTCCTGATAAGTCTCATCGCCGAACTCGTGGCAGAAAATCTGTAGTATGCAATGCTCAACAGTTCGATAGTAAATCACATTTGTTTGAGAAAGTAATCCTCAACAAGGTGGAAGACAAAGAAAAAGCATGTCACTTAACAGCCGCTGTTGAGCAAGGAAACAATGCCTCACATGTTGTCACACATTCTGGTGGCAATATGGAGATAAACGGAACCAGTTCGACAATGCCTGCATTCCACTATCAATTTCAGCAGGAACTTCCTGCTGTCCAAACATGTGGAAATGGGAGTTCTGCTTCTTCGGAAATGAAAGGTACTGATGTCAAGGCTAACAACTGTGGGACTACCTACCCTATCTATCCTCAGAACATCCCATGGCCTTATAACAATACCTGTGAAATCGAGAGAAACGAGCTAAATATTGGCCCCAGTGGTTTGTACAATAGCGGTATTGTTATCACACAAGGGCGACAGATCATCCCTGGATGGAGTTTTGGAAATGAAGAGGATGCTTCAGAAAAGCAGGAAGATGCCATGTTAGGATCAGGAAAGAATGACAATGAGGAGACCATGGGAGATGAATCCAGTTCAGGAGCAGAAAATGTTGCTCGAACAAACAGCGAGGGTGGCTTCAAAATGGATGATAGAGAAGGAACTAGCAATAAGTGTCTTGCTCCACCCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

547

Amino Acids

59.62

Weight (kDa)

5.91

Isoelectric Point (pI)

62.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18650
prunus_persica Prupe.6G212800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109471
rosa_laevigata RLG00000017740
rosa_multiflora Rmu_sc0000332.1_g000028
rosa_roxburghii Rroxscaffold_2G00134210
rosa_rugosa Rorug02G0160400
rosa_samantha Rh2BG222700 Rh2CG214300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 284, 1338
AarI CACCTGC 1 cut(s) 637
Acc16I TGCGCA 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 637
Acc65I GGTACC 1 cut(s) 513
AccB1I GGYRCC 2 cut(s) 513, 691
AccB7I CCANNNNNTGG 1 cut(s) 275
AccII CGCG 1 cut(s) 334
AciI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
AclWI GGATC 2 cut(s) 93, 1504
AcoI YGGCCR 2 cut(s) 197, 293
AcsI RAATTY 1 cut(s) 415
AcuI CTGAAG 3 cut(s) 606, 921, 1452
AfaI GTAC 6 cut(s) 127, 159, 203, 515, 1270, 1394
AfiI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
AflIII ACRYGT 2 cut(s) 1126, 1229
AgsI TTSAA 3 cut(s) 468, 674, 1588
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 5 cut(s) 353, 724, 757, 941, 1369
AluI AGCT 5 cut(s) 353, 724, 757, 941, 1369
Alw21I GWGCWC 2 cut(s) 571, 593
Alw26I GTCTC 5 cut(s) 53, 854, 910, 972, 1514
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 2 cut(s) 93, 1504
AlwNI CAGNNNCTG 2 cut(s) 629, 1100
AoxI GGCC 5 cut(s) 197, 293, 322, 1331, 1378
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 5 cut(s) 353, 376, 612, 637, 721
ApoI RAATTY 1 cut(s) 415
Asp700I GAANNNNTTC 2 cut(s) 750, 1171
Asp718I GGTACC 1 cut(s) 513
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 2 cut(s) 313, 1379
AsuHPI GGTGA 3 cut(s) 80, 164, 258
AvaII GGWCC 1 cut(s) 313
BaeGI GKGCMC 2 cut(s) 593, 694
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BanI GGYRCC 2 cut(s) 513, 691
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 2 cut(s) 828, 1074
Bbv12I GWGCWC 2 cut(s) 571, 593
BbvI GCAGC 5 cut(s) 340, 388, 599, 624, 733
BccI CCATC 3 cut(s) 143, 910, 1434
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 615, 649
BciT130I CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BcoDI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
BfmI CTRYAG 2 cut(s) 741, 996
BfuAI ACCTGC 1 cut(s) 637
BisI GCNGC 7 cut(s) 296, 354, 377, 613, 638, 722, 1098
BlsI GCNGC 7 cut(s) 297, 355, 378, 614, 639, 723, 1099
Bme1390I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 313, 1379
BmiI GGNNCC 5 cut(s) 489, 515, 693, 1168, 1381
BmrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BmrI ACTGGG 3 cut(s) 176, 340, 1377
BmsI GCATC 6 cut(s) 624, 641, 721, 934, 1453, 1474
BmuI ACTGGG 3 cut(s) 176, 340, 1377
BpiI GAAGAC 2 cut(s) 828, 1074
BpuEI CTTGAG 2 cut(s) 97, 855
BsaI GGTCTC 3 cut(s) 854, 910, 1514
BsaJI CCNNGG 5 cut(s) 194, 332, 1328, 1435, 1524
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
Bse1I ACTGG 5 cut(s) 182, 346, 1170, 1383, 1539
Bse3DI GCAATG 3 cut(s) 160, 233, 1012
BseBI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BseDI CCNNGG 5 cut(s) 194, 332, 1328, 1435, 1524
BseGI GGATG 8 cut(s) 28, 154, 736, 1323, 1431, 1445, 1468, 1600
BseLI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BseMI GCAATG 3 cut(s) 160, 233, 1012
BseMII CTCAG 1 cut(s) 1331
BseNI ACTGG 5 cut(s) 182, 346, 1170, 1383, 1539
BseRI GAGGAG 1 cut(s) 1532
BseSI GKGCMC 2 cut(s) 593, 694
BseXI GCAGC 5 cut(s) 340, 388, 599, 624, 733
Bsh1236I CGCG 1 cut(s) 334
BshFI GGCC 5 cut(s) 199, 295, 324, 1333, 1380
BshNI GGYRCC 2 cut(s) 513, 691
BsiHKAI GWGCWC 2 cut(s) 571, 593
BslFI GGGAC 1 cut(s) 1309
BslI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BsmAI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BsmFI GGGAC 1 cut(s) 1309
BsmI GAATGC 2 cut(s) 952, 1187
BsnI GGCC 5 cut(s) 199, 295, 324, 1333, 1380
Bso31I GGTCTC 3 cut(s) 854, 910, 1514
Bsp1286I GDGCHC 3 cut(s) 571, 593, 694
Bsp1407I TGTACA 1 cut(s) 1392
Bsp143I GATC 4 cut(s) 85, 926, 1428, 1496
Bsp19I CCATGG 3 cut(s) 194, 1328, 1524
BspACI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
BspANI GGCC 5 cut(s) 199, 295, 324, 1333, 1380
BspCNI CTCAG 1 cut(s) 1330
BspFNI CGCG 1 cut(s) 334
BspLI GGNNCC 5 cut(s) 489, 515, 693, 1168, 1381
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 93, 1504
BspT107I GGYRCC 2 cut(s) 513, 691
BspTNI GGTCTC 3 cut(s) 854, 910, 1514
BsrDI GCAATG 3 cut(s) 160, 233, 1012
BsrGI TGTACA 1 cut(s) 1392
BsrI ACTGG 5 cut(s) 182, 346, 1170, 1383, 1539
BssECI CCNNGG 5 cut(s) 194, 332, 1328, 1435, 1524
BssMI GATC 4 cut(s) 85, 926, 1428, 1496
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 3 cut(s) 194, 1328, 1524
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Bst4CI ACNGT 5 cut(s) 411, 834, 850, 1017, 1292
Bst6I CTCTTC 4 cut(s) 5, 625, 852, 1452
BstAPI GCANNNNNTGC 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 1392
BstC8I GCNNGC 3 cut(s) 37, 326, 1185
BstDEI CTNAG 2 cut(s) 708, 1317
BstDSI CCRYGG 4 cut(s) 194, 332, 1328, 1524
BstENI CCTNNNNNAGG 1 cut(s) 1058
BstF5I GGATG 8 cut(s) 28, 154, 736, 1323, 1431, 1445, 1468, 1600
BstFNI CGCG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 4 cut(s) 88, 929, 1431, 1499
BstMAI GTCTC 5 cut(s) 53, 854, 910, 972, 1514
BstMBI GATC 4 cut(s) 85, 926, 1428, 1496
BstNI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
BstNSI RCATGY 4 cut(s) 208, 1086, 1130, 1233
BstSCI CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1435
BstSFI CTRYAG 2 cut(s) 741, 996
BstSLI GKGCMC 2 cut(s) 593, 694
BstUI CGCG 1 cut(s) 334
BstV1I GCAGC 5 cut(s) 340, 388, 599, 624, 733
BstV2I GAAGAC 2 cut(s) 828, 1074
BstXI CCANNNNNNTGG 1 cut(s) 1232
BsuRI GGCC 5 cut(s) 199, 295, 324, 1333, 1380
BtgI CCRYGG 4 cut(s) 194, 332, 1328, 1524
BtgZI GCGATG 2 cut(s) 618, 956
BtsCI GGATG 8 cut(s) 28, 154, 736, 1323, 1431, 1445, 1468, 1600
BtsI GCAGTG 2 cut(s) 864, 954
BtsIMutI CAGTG 4 cut(s) 132, 864, 954, 1390
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 3 cut(s) 37, 326, 1185
CaiI CAGNNNCTG 2 cut(s) 629, 1100
CfoI GCGC 1 cut(s) 664
Cfr13I GGNCC 2 cut(s) 313, 1379
Cfr42I CCGCGG 1 cut(s) 335
Csp6I GTAC 6 cut(s) 126, 158, 202, 514, 1269, 1393
CviQI GTAC 6 cut(s) 126, 158, 202, 514, 1269, 1393
DdeI CTNAG 2 cut(s) 708, 1317
DpnI GATC 4 cut(s) 87, 928, 1430, 1498
DpnII GATC 4 cut(s) 85, 926, 1428, 1496
EaeI YGGCCR 2 cut(s) 197, 293
Eam1104I CTCTTC 4 cut(s) 5, 625, 852, 1452
EarI CTCTTC 4 cut(s) 5, 625, 852, 1452
Eco130I CCWWGG 3 cut(s) 194, 1328, 1524
Eco31I GGTCTC 3 cut(s) 854, 910, 1514
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 3 cut(s) 606, 921, 1452
EcoNI CCTNNNNNAGG 1 cut(s) 1058
EcoRII CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
EcoT14I CCWWGG 3 cut(s) 194, 1328, 1524
ErhI CCWWGG 3 cut(s) 194, 1328, 1524
FaqI GGGAC 1 cut(s) 1309
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 7 cut(s) 296, 354, 377, 613, 638, 722, 1098
FokI GGATG 8 cut(s) 35, 161, 743, 1310, 1418, 1452, 1475, 1607
Fsp4HI GCNGC 7 cut(s) 296, 354, 377, 613, 638, 722, 1098
FspBI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
FspI TGCGCA 1 cut(s) 663
GlaI GCGC 1 cut(s) 663
GluI GCNGC 7 cut(s) 296, 354, 377, 613, 638, 722, 1098
HaeIII GGCC 5 cut(s) 199, 295, 324, 1333, 1380
HhaI GCGC 1 cut(s) 664
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HinfI GANTC 3 cut(s) 545, 562, 1535
HphI GGTGA 3 cut(s) 80, 164, 258
Hpy166II GTNNAC 1 cut(s) 591
Hpy188I TCNGA 5 cut(s) 148, 787, 1258, 1320, 1471
Hpy188III TCNNGA 7 cut(s) 50, 76, 243, 959, 1357, 1500, 1545
Hpy8I GTNNAC 1 cut(s) 591
HpyAV CCTTC 4 cut(s) 13, 153, 171, 1598
HpyCH4III ACNGT 5 cut(s) 411, 834, 850, 1017, 1292
HpyF3I CTNAG 2 cut(s) 708, 1317
HspAI GCGC 1 cut(s) 662
KpnI GGTACC 1 cut(s) 517
KspI CCGCGG 1 cut(s) 335
Kzo9I GATC 4 cut(s) 85, 926, 1428, 1496
LmnI GCTCC 5 cut(s) 223, 361, 440, 1547, 1633
Lsp1109I GCAGC 5 cut(s) 340, 388, 599, 624, 733
LweI GCATC 6 cut(s) 624, 641, 721, 934, 1453, 1474
MaeI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
MaeIII GTNAC 5 cut(s) 170, 184, 790, 1085, 1132
MalI GATC 4 cut(s) 87, 928, 1430, 1498
MboI GATC 4 cut(s) 85, 926, 1428, 1496
MhlI GDGCHC 3 cut(s) 571, 593, 694
MluCI AATT 5 cut(s) 415, 493, 681, 805, 1199
MmeI TCCRAC 1 cut(s) 418
MroXI GAANNNNTTC 2 cut(s) 750, 1171
MseI TTAA 4 cut(s) 812, 843, 876, 1091
MslI CAYNNNNRTG 1 cut(s) 1143
MspA1I CMGCKG 4 cut(s) 334, 353, 941, 1100
MspR9I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
Mva1269I GAATGC 2 cut(s) 952, 1187
MvaI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1437
MvnI CGCG 1 cut(s) 334
NcoI CCATGG 3 cut(s) 194, 1328, 1524
NdeI CATATG 1 cut(s) 595
NdeII GATC 4 cut(s) 85, 926, 1428, 1496
NlaIV GGNNCC 5 cut(s) 489, 515, 693, 1168, 1381
NmuCI GTSAC 3 cut(s) 170, 1085, 1132
NsbI TGCGCA 1 cut(s) 663
NspI RCATGY 4 cut(s) 208, 1086, 1130, 1233
PaqCI CACCTGC 1 cut(s) 637
PciI ACATGT 2 cut(s) 1126, 1229
PcsI WCGNNNNNNNCGW 2 cut(s) 979, 1362
PctI GAATGC 2 cut(s) 952, 1187
PdmI GAANNNNTTC 2 cut(s) 750, 1171
PfeI GAWTC 3 cut(s) 545, 562, 1535
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 604
PkrI GCNGC 7 cut(s) 297, 355, 378, 614, 639, 723, 1099
PscI ACATGT 2 cut(s) 1126, 1229
PsiI TTATAA 2 cut(s) 284, 1338
Psp6I CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
PspGI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1435
PspN4I GGNNCC 5 cut(s) 489, 515, 693, 1168, 1381
PspPI GGNCC 2 cut(s) 313, 1379
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 2 cut(s) 629, 1100
PvuII CAGCTG 2 cut(s) 353, 941
RsaI GTAC 6 cut(s) 127, 159, 203, 515, 1270, 1394
RsaNI GTAC 6 cut(s) 126, 158, 202, 514, 1269, 1393
RseI CAYNNNNRTG 1 cut(s) 1143
SacII CCGCGG 1 cut(s) 335
SaqAI TTAA 4 cut(s) 812, 843, 876, 1091
SatI GCNGC 7 cut(s) 296, 354, 377, 613, 638, 722, 1098
Sau3AI GATC 4 cut(s) 85, 926, 1428, 1496
Sau96I GGNCC 2 cut(s) 313, 1379
ScrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1437
SduI GDGCHC 3 cut(s) 571, 593, 694
SfaNI GCATC 6 cut(s) 624, 641, 721, 934, 1453, 1474
SfcI CTRYAG 2 cut(s) 741, 996
Sfr303I CCGCGG 1 cut(s) 335
SgrBI CCGCGG 1 cut(s) 335
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 2 cut(s) 76, 870
SmoI CTYRAG 2 cut(s) 76, 870
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 5 cut(s) 415, 493, 681, 805, 1199
SsiI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
SspI AATATT 1 cut(s) 1375
SspMI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
StyD4I CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1435
StyI CCWWGG 3 cut(s) 194, 1328, 1524
TaaI ACNGT 5 cut(s) 411, 834, 850, 1017, 1292
TaqI TCGA 6 cut(s) 565, 822, 1020, 1175, 1356, 1564
TasI AATT 5 cut(s) 415, 493, 681, 805, 1199
TatI WGTACW 2 cut(s) 125, 1392
TauI GCSGC 2 cut(s) 298, 1100
TfiI GAWTC 3 cut(s) 545, 562, 1535
Tru1I TTAA 4 cut(s) 812, 843, 876, 1091
Tru9I TTAA 4 cut(s) 812, 843, 876, 1091
TscAI CASTG 4 cut(s) 139, 871, 954, 1390
TseFI GTSAC 3 cut(s) 170, 1085, 1132
TseI GCWGC 5 cut(s) 353, 376, 612, 637, 721
Tsp45I GTSAC 3 cut(s) 170, 1085, 1132
TspDTI ATGAA 4 cut(s) 528, 1277, 1470, 1548
TspGWI ACGGA 1 cut(s) 1179
TspRI CASTG 4 cut(s) 139, 871, 954, 1390
Van91I CCANNNNNTGG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 1058
XapI RAATTY 1 cut(s) 415
XbaI TCTAGA 1 cut(s) 242
XceI RCATGY 4 cut(s) 208, 1086, 1130, 1233
XmnI GAANNNNTTC 2 cut(s) 750, 1171
XspI CTAG 5 cut(s) 45, 243, 642, 1611, 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.