Rh2BG222700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018777
Physical Location & Seq
Reverse (-)
21543320 .. 21545922
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG222700.1

Sequence Viewer

Length: 1641 bp
ATGGAGAAGAAGAGGAAGAAGGATGAGAAAGTTGCTTGCACCACTAGTCAGGAACCAGAGACAGACCACCATTTCTTGAGCAAGGATCACCAAAGCCAAGACAATGCTGAACCTCTAACAAAGAGTACAAACACTGATAGTCCTTCGAATGGCAACGTACCTTCCCTATGTCACCAATACCCAGTAACTTTTCCATGGCCGTACGCGCCTCAACATGGTGGGGAGCAATGTCCTTTGGTGTCTAGACCCTTTTTTGCAACTCAATCACCATTGCCTGGCAATATAAGCCAATGGCCGCAGTTTCCACATCAGGTCCAGCATGGCCAGCCACCGCGGAACTATTCCCAGTCAGCTGCTCCCTTTTGGCTACCACAGCAGCCTGGTTATCCATTTTCTGCCTCCAATGCACCGTCAACTTTCCAGCCATTTGTTGCTGTTGGAGCAACTGATATTAGTGGTGAAACATCTACAACAAACCAAAATCAGGTTCCCAATTTCTATTACCATGTTGGGTACCCATATCCAGGCTTTCCAGGTTCATGTGATTCCTTATCTTGGTGGAATCAAGCACAAGCACCACAACCTCTGTGCACATATTCTTTTCCAGGAGGCTGCATCTCTTCACCACCTGCATCACTGCCTAGTTGTTCTGCAACTCTTGGGCAAACCTTTGAAAAAGGAATTATCAGGGCACCAGCAAAACTTTCTAAGAAGCACCAGCAGCTTTGGGATGCTCAATCTGCAGAAAATGTTCAGCTATGGAGTGCAATCAACCACTTGCAATCAGAAGTAACTGATTACAAGAATTGCTTAACAAGTCTCGAAGCAGAACTGTCTTCTCTTAAAGCAGTGGCAGAAGAGACCACTGCTCAAGTTAGTGGGGCTGTTTTACCTGCAAAACCATCAAAGAGAGGGAGACCAAAGAGATCAGTTGCTTCAGCTGATGCAGTGCATTCTCCTGATAAGTCTCATCGCCGAGCTTGTGGCAGAAAATCTGTAGTATGCAATGCTCAACAGTTTGACAGTAAATCACATTTGTTTGAGAAAGTAATCCTCAACAAGGTGGAAGACAAAGAAAAAGCATGTCACTTAACAGCCGCTGTTGAGCAAGGAAACAATGCCTCACATGTTGTCACACATTCTGGTGGCCATATGGAGATAAATGGAACCAGTTCGACAATGCCTGCATTCCACTATCAATTTCAGCAGGAACTACCTGCTGTCCAAACATATGGAAATGGGAGTTCTGCTTCTTCGGAAATGAAAGTTTCTGATGTCAAGGCTAACAACTGTAGGACTACCTACCCTGTCTATCCTCAGAACATCCCATGGCCTTATAACAATACCTCTGAAATTGAGAGAAACGAGCTAAATATTGGCCCCAATGGTTTGTACAATAGCGGTATTGTTATACAAGGGGGACAGATCATCCCTGGATGGAGTTTTGGAAATGAAGAGGACGCTTCAGAAAAGCAGGAAGATGCCATGTTAGGATCAGGAAAGAATGACAATGAGGAGACCATGGGAGATGAATCTAGTTCAGGAGCAGAAAAAGTTGCTCGAACAAACAATGATGGTGGCTTCAAAATGGATGATAGAGAAGGAACTAGCAAAAAGTGTCTTGCTCCACCCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

546

Amino Acids

59.55

Weight (kDa)

6.06

Isoelectric Point (pI)

62.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18650
prunus_persica Prupe.6G212800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109471
rosa_laevigata RLG00000017740
rosa_multiflora Rmu_sc0000332.1_g000028
rosa_roxburghii Rroxscaffold_2G00134210
rosa_rugosa Rorug02G0160400
rosa_samantha Rh2BG222700 Rh2CG214300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1338
AarI CACCTGC 1 cut(s) 637
Acc36I ACCTGC 3 cut(s) 637, 901, 1225
Acc65I GGTACC 1 cut(s) 513
AccB1I GGYRCC 2 cut(s) 513, 691
AccB7I CCANNNNNTGG 1 cut(s) 275
AccII CGCG 2 cut(s) 206, 334
AciI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
AclWI GGATC 2 cut(s) 93, 1501
AcoI YGGCCR 4 cut(s) 197, 293, 322, 1147
AcuI CTGAAG 2 cut(s) 921, 1449
AfaI GTAC 5 cut(s) 127, 159, 203, 515, 1394
AfiI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
AflIII ACRYGT 1 cut(s) 1126
AgsI TTSAA 2 cut(s) 674, 1585
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1432
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 6 cut(s) 353, 724, 757, 941, 980, 1369
AluI AGCT 6 cut(s) 353, 724, 757, 941, 980, 1369
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 6 cut(s) 53, 824, 854, 910, 972, 1511
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 2 cut(s) 93, 1501
AlwNI CAGNNNCTG 1 cut(s) 1100
AoxI GGCC 6 cut(s) 197, 293, 322, 1147, 1331, 1378
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 4 cut(s) 353, 376, 612, 721
Asp700I GAANNNNTTC 2 cut(s) 750, 1171
Asp718I GGTACC 1 cut(s) 513
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 313, 1379
AsuHPI GGTGA 5 cut(s) 80, 164, 258, 470, 615
AsuII TTCGAA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 313
BaeGI GKGCMC 2 cut(s) 593, 694
BalI TGGCCA 2 cut(s) 324, 1149
BanI GGYRCC 2 cut(s) 513, 691
BbsI GAAGAC 2 cut(s) 828, 1074
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 4 cut(s) 340, 388, 599, 733
BccI CCATC 3 cut(s) 910, 1431, 1568
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1434
BcoDI GTCTC 6 cut(s) 53, 824, 854, 910, 972, 1511
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 6 cut(s) 45, 243, 642, 1536, 1608, 1639
BfmI CTRYAG 3 cut(s) 741, 996, 1291
BfuAI ACCTGC 3 cut(s) 637, 901, 1225
BisI GCNGC 6 cut(s) 296, 354, 377, 613, 722, 1098
BlsI GCNGC 6 cut(s) 297, 355, 378, 614, 723, 1099
Bme1390I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1434
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 313, 1379
BmiI GGNNCC 6 cut(s) 54, 489, 515, 693, 1168, 1381
BmrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1434
BmrI ACTGGG 2 cut(s) 176, 340
BmsI GCATC 5 cut(s) 624, 641, 721, 934, 1471
BmuI ACTGGG 2 cut(s) 176, 340
BpiI GAAGAC 2 cut(s) 828, 1074
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 2 cut(s) 97, 855
BsaI GGTCTC 3 cut(s) 854, 910, 1511
BsaJI CCNNGG 5 cut(s) 194, 332, 1328, 1432, 1521
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
Bse1I ACTGG 3 cut(s) 182, 346, 1170
Bse3DI GCAATG 3 cut(s) 233, 269, 1012
BseBI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1434
BseDI CCNNGG 5 cut(s) 194, 332, 1328, 1432, 1521
BseGI GGATG 6 cut(s) 28, 736, 1323, 1428, 1442, 1597
BseLI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BseMI GCAATG 3 cut(s) 233, 269, 1012
BseMII CTCAG 1 cut(s) 1331
BseNI ACTGG 3 cut(s) 182, 346, 1170
BseRI GAGGAG 1 cut(s) 1529
BseSI GKGCMC 2 cut(s) 593, 694
BseXI GCAGC 4 cut(s) 340, 388, 599, 733
Bsh1236I CGCG 2 cut(s) 206, 334
BshFI GGCC 6 cut(s) 199, 295, 324, 1149, 1333, 1380
BshNI GGYRCC 2 cut(s) 513, 691
BsiHKAI GWGCWC 1 cut(s) 593
BsiWI CGTACG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 1434
BslI CCNNNNNNNGG 7 cut(s) 149, 215, 275, 484, 524, 555, 1060
BsmAI GTCTC 6 cut(s) 53, 824, 854, 910, 972, 1511
BsmFI GGGAC 1 cut(s) 1434
BsmI GAATGC 2 cut(s) 952, 1187
BsnI GGCC 6 cut(s) 199, 295, 324, 1149, 1333, 1380
Bso31I GGTCTC 3 cut(s) 854, 910, 1511
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 593, 694
Bsp1407I TGTACA 1 cut(s) 1392
Bsp143I GATC 4 cut(s) 85, 926, 1425, 1493
Bsp19I CCATGG 3 cut(s) 194, 1328, 1521
BspACI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
BspANI GGCC 6 cut(s) 199, 295, 324, 1149, 1333, 1380
BspCNI CTCAG 1 cut(s) 1330
BspFNI CGCG 2 cut(s) 206, 334
BspLI GGNNCC 6 cut(s) 54, 489, 515, 693, 1168, 1381
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 3 cut(s) 637, 901, 1225
BspPI GGATC 2 cut(s) 93, 1501
BspT104I TTCGAA 1 cut(s) 146
BspT107I GGYRCC 2 cut(s) 513, 691
BspTNI GGTCTC 3 cut(s) 854, 910, 1511
BsrDI GCAATG 3 cut(s) 233, 269, 1012
BsrGI TGTACA 1 cut(s) 1392
BsrI ACTGG 3 cut(s) 182, 346, 1170
BssECI CCNNGG 5 cut(s) 194, 332, 1328, 1432, 1521
BssMI GATC 4 cut(s) 85, 926, 1425, 1493
BssT1I CCWWGG 3 cut(s) 194, 1328, 1521
Bst2UI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1434
Bst4CI ACNGT 5 cut(s) 411, 834, 1017, 1025, 1292
Bst6I CTCTTC 4 cut(s) 5, 625, 852, 1449
BstAUI TGTACA 1 cut(s) 1392
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 37, 326, 1185
BstDEI CTNAG 2 cut(s) 708, 1317
BstDSI CCRYGG 4 cut(s) 194, 332, 1328, 1521
BstENI CCTNNNNNAGG 1 cut(s) 1058
BstF5I GGATG 6 cut(s) 28, 736, 1323, 1428, 1442, 1597
BstFNI CGCG 2 cut(s) 206, 334
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 4 cut(s) 88, 929, 1428, 1496
BstMAI GTCTC 6 cut(s) 53, 824, 854, 910, 972, 1511
BstMBI GATC 4 cut(s) 85, 926, 1425, 1493
BstMWI GCNNNNNNNGC 8 cut(s) 205, 285, 325, 373, 404, 440, 721, 740
BstNI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1434
BstNSI RCATGY 2 cut(s) 1086, 1130
BstSCI CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1432
BstSFI CTRYAG 3 cut(s) 741, 996, 1291
BstSLI GKGCMC 2 cut(s) 593, 694
BstUI CGCG 2 cut(s) 206, 334
BstV1I GCAGC 4 cut(s) 340, 388, 599, 733
BstV2I GAAGAC 2 cut(s) 828, 1074
BstXI CCANNNNNNTGG 1 cut(s) 1232
BsuRI GGCC 6 cut(s) 199, 295, 324, 1149, 1333, 1380
BtgI CCRYGG 4 cut(s) 194, 332, 1328, 1521
BtgZI GCGATG 1 cut(s) 956
BtsCI GGATG 6 cut(s) 28, 736, 1323, 1428, 1442, 1597
BtsI GCAGTG 4 cut(s) 635, 855, 864, 954
BtsIMutI CAGTG 5 cut(s) 132, 635, 855, 864, 954
BveI ACCTGC 3 cut(s) 637, 901, 1225
Cac8I GCNNGC 3 cut(s) 37, 326, 1185
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 313, 1379
Cfr42I CCGCGG 1 cut(s) 335
CseI GACGC 1 cut(s) 1469
Csp6I GTAC 5 cut(s) 126, 158, 202, 514, 1393
CspCI CAANNNNNGTGG 2 cut(s) 1558, 1593
CviQI GTAC 5 cut(s) 126, 158, 202, 514, 1393
DdeI CTNAG 2 cut(s) 708, 1317
DpnI GATC 4 cut(s) 87, 928, 1427, 1495
DpnII GATC 4 cut(s) 85, 926, 1425, 1493
EaeI YGGCCR 4 cut(s) 197, 293, 322, 1147
Eam1104I CTCTTC 4 cut(s) 5, 625, 852, 1449
EarI CTCTTC 4 cut(s) 5, 625, 852, 1449
Eco130I CCWWGG 3 cut(s) 194, 1328, 1521
Eco31I GGTCTC 3 cut(s) 854, 910, 1511
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 921, 1449
EcoNI CCTNNNNNAGG 1 cut(s) 1058
EcoRII CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1432
EcoT14I CCWWGG 3 cut(s) 194, 1328, 1521
ErhI CCWWGG 3 cut(s) 194, 1328, 1521
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 1434
FauNDI CATATG 2 cut(s) 1152, 1231
Fnu4HI GCNGC 6 cut(s) 296, 354, 377, 613, 722, 1098
FokI GGATG 6 cut(s) 35, 743, 1310, 1415, 1449, 1604
Fsp4HI GCNGC 6 cut(s) 296, 354, 377, 613, 722, 1098
FspBI CTAG 6 cut(s) 45, 243, 642, 1536, 1608, 1639
GlaI GCGC 1 cut(s) 207
GluI GCNGC 6 cut(s) 296, 354, 377, 613, 722, 1098
HaeIII GGCC 6 cut(s) 199, 295, 324, 1149, 1333, 1380
HgaI GACGC 1 cut(s) 1469
HhaI GCGC 1 cut(s) 208
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 3 cut(s) 545, 562, 1532
HphI GGTGA 5 cut(s) 80, 164, 258, 470, 615
Hpy166II GTNNAC 2 cut(s) 414, 591
Hpy188I TCNGA 6 cut(s) 787, 1258, 1273, 1320, 1351, 1468
Hpy188III TCNNGA 7 cut(s) 50, 76, 243, 821, 959, 1497, 1542
Hpy8I GTNNAC 2 cut(s) 414, 591
HpyAV CCTTC 4 cut(s) 13, 153, 171, 1595
HpyCH4III ACNGT 5 cut(s) 411, 834, 1017, 1025, 1292
HpyCH4IV ACGT 1 cut(s) 156
HpyF10VI GCNNNNNNNGC 8 cut(s) 205, 285, 325, 373, 404, 440, 721, 740
HpyF3I CTNAG 2 cut(s) 708, 1317
HpySE526I ACGT 1 cut(s) 156
HspAI GCGC 1 cut(s) 206
KpnI GGTACC 1 cut(s) 517
KspI CCGCGG 1 cut(s) 335
Kzo9I GATC 4 cut(s) 85, 926, 1425, 1493
LmnI GCTCC 5 cut(s) 223, 361, 440, 1544, 1630
Lsp1109I GCAGC 4 cut(s) 340, 388, 599, 733
LweI GCATC 5 cut(s) 624, 641, 721, 934, 1471
MaeI CTAG 6 cut(s) 45, 243, 642, 1536, 1608, 1639
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 5 cut(s) 170, 184, 790, 1085, 1132
MalI GATC 4 cut(s) 87, 928, 1427, 1495
MboI GATC 4 cut(s) 85, 926, 1425, 1493
MhlI GDGCHC 2 cut(s) 593, 694
MlsI TGGCCA 2 cut(s) 324, 1149
MluCI AATT 5 cut(s) 493, 681, 805, 1199, 1353
MluNI TGGCCA 2 cut(s) 324, 1149
MmeI TCCRAC 1 cut(s) 418
Mox20I TGGCCA 2 cut(s) 324, 1149
MroXI GAANNNNTTC 2 cut(s) 750, 1171
MscI TGGCCA 2 cut(s) 324, 1149
MseI TTAA 3 cut(s) 812, 843, 1091
MslI CAYNNNNRTG 1 cut(s) 1143
Msp20I TGGCCA 2 cut(s) 324, 1149
MspA1I CMGCKG 4 cut(s) 334, 353, 941, 1100
MspR9I CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1434
Mva1269I GAATGC 2 cut(s) 952, 1187
MvaI CCWGG 6 cut(s) 276, 381, 525, 534, 606, 1434
MvnI CGCG 2 cut(s) 206, 334
MwoI GCNNNNNNNGC 8 cut(s) 205, 285, 325, 373, 404, 440, 721, 740
NcoI CCATGG 3 cut(s) 194, 1328, 1521
NdeI CATATG 2 cut(s) 1152, 1231
NdeII GATC 4 cut(s) 85, 926, 1425, 1493
NlaIV GGNNCC 6 cut(s) 54, 489, 515, 693, 1168, 1381
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 3 cut(s) 170, 1085, 1132
NspI RCATGY 2 cut(s) 1086, 1130
NspV TTCGAA 1 cut(s) 146
PaqCI CACCTGC 1 cut(s) 637
PciI ACATGT 1 cut(s) 1126
PctI GAATGC 2 cut(s) 952, 1187
PdmI GAANNNNTTC 2 cut(s) 750, 1171
PfeI GAWTC 3 cut(s) 545, 562, 1532
Pfl23II CGTACG 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 604
PkrI GCNGC 6 cut(s) 297, 355, 378, 614, 723, 1099
PscI ACATGT 1 cut(s) 1126
PsiI TTATAA 1 cut(s) 1338
Psp6I CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1432
PspGI CCWGG 6 cut(s) 274, 379, 523, 532, 604, 1432
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 6 cut(s) 54, 489, 515, 693, 1168, 1381
PspPI GGNCC 2 cut(s) 313, 1379
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 1100
PvuII CAGCTG 2 cut(s) 353, 941
RsaI GTAC 5 cut(s) 127, 159, 203, 515, 1394
RsaNI GTAC 5 cut(s) 126, 158, 202, 514, 1393
RseI CAYNNNNRTG 1 cut(s) 1143
SacII CCGCGG 1 cut(s) 335
SaqAI TTAA 3 cut(s) 812, 843, 1091
SatI GCNGC 6 cut(s) 296, 354, 377, 613, 722, 1098
Sau3AI GATC 4 cut(s) 85, 926, 1425, 1493
Sau96I GGNCC 2 cut(s) 313, 1379
ScrFI CCNGG 6 cut(s) 276, 381, 525, 534, 606, 1434
SduI GDGCHC 2 cut(s) 593, 694
SfaNI GCATC 5 cut(s) 624, 641, 721, 934, 1471
SfcI CTRYAG 3 cut(s) 741, 996, 1291
Sfr303I CCGCGG 1 cut(s) 335
SfuI TTCGAA 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 335
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 2 cut(s) 76, 870
SmoI CTYRAG 2 cut(s) 76, 870
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 5 cut(s) 493, 681, 805, 1199, 1353
SsiI CCGC 5 cut(s) 296, 332, 334, 1098, 1401
SspI AATATT 1 cut(s) 1375
SspMI CTAG 6 cut(s) 45, 243, 642, 1536, 1608, 1639
StyD4I CCNGG 6 cut(s) 274, 379, 523, 532, 604, 1432
StyI CCWWGG 3 cut(s) 194, 1328, 1521
TaaI ACNGT 5 cut(s) 411, 834, 1017, 1025, 1292
TaiI ACGT 1 cut(s) 159
TaqI TCGA 4 cut(s) 146, 822, 1175, 1561
TasI AATT 5 cut(s) 493, 681, 805, 1199, 1353
TatI WGTACW 2 cut(s) 125, 1392
TauI GCSGC 2 cut(s) 298, 1100
TfiI GAWTC 3 cut(s) 545, 562, 1532
Tru1I TTAA 3 cut(s) 812, 843, 1091
Tru9I TTAA 3 cut(s) 812, 843, 1091
TscAI CASTG 5 cut(s) 139, 642, 855, 871, 954
TseFI GTSAC 3 cut(s) 170, 1085, 1132
TseI GCWGC 4 cut(s) 353, 376, 612, 721
Tsp45I GTSAC 3 cut(s) 170, 1085, 1132
TspDTI ATGAA 4 cut(s) 528, 1277, 1467, 1545
TspRI CASTG 5 cut(s) 139, 642, 855, 871, 954
Van91I CCANNNNNTGG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 1058
XbaI TCTAGA 1 cut(s) 242
XceI RCATGY 2 cut(s) 1086, 1130
XmnI GAANNNNTTC 2 cut(s) 750, 1171
XspI CTAG 6 cut(s) 45, 243, 642, 1536, 1608, 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.