RLG00000018080

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020537
Physical Location & Seq
Reverse (-)
25103210 .. 25104345
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018080

Sequence Viewer

Length: 588 bp
ATGTTGAAAGAAGTTGGAAATGACTACTTTGATGATCTACTCTCAAGTTCATTTCTTCAACATCGATATGGCAACGATTTCTTCATGCACGACCTTATTAGTTATTTGGCAAAGTTTGTATATGGAAAATTCTGTGTTAGACTGGAGGACAGTGCCTCCTTCCAACCAAGAGTTGAAGAAGCCTTGGCATTTGCGAGAAGTACTTCATATGGGCTTCATAATATTGTCTATGTCGAAGATGCTTTAGAGGCCAATATAGGGGAGAAGAAGTATCTTGATGGACTGAGTTTCTTCATGAAGAGGGGCATAAGCTTTCCTAGTTGGTTAGCAAATTATTCTTTCCAGTTGGTGTATCTTCAACTTTCCAATTGTATTAATTCTTGCTTTTTGCCACCACTGGAGCAGCTACCCTTCCTCCGGGATCTTCATGTTATTCAGGTCGTGAGTTTTACTGGTAAATGGAGAGTCTTCTCCGCGATTGATGGACGAAGGAGAGCTCTCAAGTCTACTTCGATAGGTCCATTTGTTGTTCAAGTCTTTGAGCTTGTCTTTCACGCATTTGCACCACTCTCCAAAAATGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

196

Amino Acids

22.43

Weight (kDa)

6.51

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 2 - 36 1.8e-06 Disease resistance protein Winged helix domain
LRR_R13L1-DRL21 PF25019 101 - 145 1.9e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020537)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0387391
rosa_laevigata RLG00000018080 RLG00000018082
rosa_multiflora Rmu_sc0004283.1_g000014 Rmu_sc0009639.1_g000004
rosa_rugosa Rorug02G0188900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 506
AccII CGCG 1 cut(s) 476
AciI CCGC 1 cut(s) 474
AclWI GGATC 1 cut(s) 429
AcsI RAATTY 1 cut(s) 128
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 2 cut(s) 258, 417
AgsI TTSAA 5 cut(s) 7, 59, 176, 359, 533
AluBI AGCT 4 cut(s) 312, 406, 497, 544
AluI AGCT 4 cut(s) 312, 406, 497, 544
Alw21I GWGCWC 1 cut(s) 499
AlwI GGATC 1 cut(s) 429
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 1 cut(s) 128
AseI ATTAAT 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 518
AsuC2I CCSGG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 499
BbvI GCAGC 1 cut(s) 415
BccI CCATC 2 cut(s) 272, 476
BcnI CCSGG 1 cut(s) 419
BfaI CTAG 1 cut(s) 318
BisI GCNGC 1 cut(s) 404
BlsI GCNGC 1 cut(s) 405
BmcAI AGTACT 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 460
BpmI CTGGAG 2 cut(s) 164, 419
BpuEI CTTGAG 2 cut(s) 28, 485
BpuMI CCSGG 1 cut(s) 419
Bsa29I ATCGAT 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 183
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 254, 284, 399, 429
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 417
Bse1I ACTGG 4 cut(s) 147, 343, 402, 457
BseCI ATCGAT 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 258, 417
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 4 cut(s) 147, 343, 402, 457
BseXI GCAGC 1 cut(s) 415
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 499
BsiSI CCGG 1 cut(s) 418
BslI CCNNNNNNNGG 2 cut(s) 258, 417
BsnI GGCC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 499
Bsp143I GATC 2 cut(s) 34, 421
BspACI CCGC 1 cut(s) 474
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 1 cut(s) 276
BspDI ATCGAT 1 cut(s) 64
BspFNI CGCG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 294
BspPI GGATC 1 cut(s) 429
BsrI ACTGG 4 cut(s) 147, 343, 402, 457
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 2 cut(s) 34, 421
BssT1I CCWWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 152
Bst6I CTCTTC 1 cut(s) 293
BstDEI CTNAG 1 cut(s) 284
BstFNI CGCG 1 cut(s) 476
BstKTI GATC 2 cut(s) 37, 424
BstMBI GATC 2 cut(s) 34, 421
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 417
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 460
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 64
BsuRI GGCC 1 cut(s) 251
BsuTUI ATCGAT 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 157, 395
CciI TCATGA 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 518
ClaI ATCGAT 1 cut(s) 64
Csp6I GTAC 1 cut(s) 201
CviAII CATG 3 cut(s) 85, 295, 428
CviJI RGCY 7 cut(s) 182, 214, 251, 312, 406, 497, 544
CviKI_1 RGCY 7 cut(s) 182, 214, 251, 312, 406, 497, 544
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 284
DpnI GATC 2 cut(s) 36, 423
DpnII GATC 2 cut(s) 34, 421
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 518
Eco53kI GAGCTC 1 cut(s) 497
EcoICRI GAGCTC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 88, 298, 431
FatI CATG 3 cut(s) 84, 294, 427
FauNDI CATATG 1 cut(s) 208
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 1 cut(s) 404
FspBI CTAG 1 cut(s) 318
GluI GCNGC 1 cut(s) 404
GsuI CTGGAG 2 cut(s) 164, 419
HaeIII GGCC 1 cut(s) 251
HapII CCGG 1 cut(s) 418
Hin1II CATG 3 cut(s) 88, 298, 431
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 1 cut(s) 465
HpaII CCGG 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 507
Hpy188III TCNNGA 3 cut(s) 275, 295, 442
Hpy8I GTNNAC 1 cut(s) 507
HpyAV CCTTC 3 cut(s) 169, 421, 483
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 88, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 1 cut(s) 284
Hsp92II CATG 3 cut(s) 88, 298, 431
Kzo9I GATC 2 cut(s) 34, 421
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 6 cut(s) 128, 356, 383, 422, 431, 438
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 318
MalI GATC 2 cut(s) 36, 423
MboI GATC 2 cut(s) 34, 421
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 4 cut(s) 128, 331, 367, 376
MlyI GAGTC 1 cut(s) 474
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 5 cut(s) 139, 166, 241, 294, 425
MroXI GAANNNNTTC 1 cut(s) 202
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 66
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 1 cut(s) 367
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 419
NdeI CATATG 1 cut(s) 208
NdeII GATC 2 cut(s) 34, 421
NlaIII CATG 3 cut(s) 88, 298, 431
PagI TCATGA 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 202
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 1 cut(s) 405
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 499
PspPI GGNCC 1 cut(s) 518
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 66
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 2 cut(s) 34, 421
Sau96I GGNCC 1 cut(s) 518
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 1 cut(s) 474
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 1 cut(s) 499
SetI ASST 7 cut(s) 96, 314, 408, 441, 499, 520, 546
SfaNI GCATC 1 cut(s) 229
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 43, 500
SmoI CTYRAG 2 cut(s) 43, 500
Sse9I AATT 4 cut(s) 128, 331, 367, 376
SsiI CCGC 1 cut(s) 474
SspI AATATT 1 cut(s) 223
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 499
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 3 cut(s) 64, 234, 512
TasI AATT 4 cut(s) 128, 331, 367, 376
TatI WGTACW 1 cut(s) 200
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 157, 402
TseI GCWGC 1 cut(s) 403
TspDTI ATGAA 7 cut(s) 39, 73, 195, 206, 283, 311, 416
TspRI CASTG 2 cut(s) 157, 402
VpaK11BI GGWCC 1 cut(s) 518
VspI ATTAAT 1 cut(s) 375
XapI RAATTY 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 202
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.