RLG00000018082

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020537
Physical Location & Seq
Reverse (-)
25118985 .. 25120120
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018082

Sequence Viewer

Length: 555 bp
ATGTTGAAAGAAGTTGGAAATGACTACTTTGATGATCTACTCTCAAGTTCATTTCTTCAACATCGATATGGCAACGATTTCTTCATGCACGACCTTATTAGTTATTTGGCAAAGTTTGTATATGGAAAATTCTGTGTTAGACTGGAGGACAGTGCCTCCTTCCAACCAAGAAGATACAAAGTTAGGAAGGATGCCACCACAGTACCACATATGAGAAAAATGAAAGATCTTCAAACGTCAGGTGATTTTGTGTTGGGAAAGAATTCAGAGGTTGGTTTTGCAGAGTTGAAGAAGCCTTGGCATTTGCGAGAATTGGTTTATCTTCAACTTTCCAATTGTATTAATTCTTGCTTTTTGCCACCACTGGAGCAGCTACCCTTCCTCCGGGATCTTCATGTTATTCAGGTCGTGAGTTTTACTGGTAAATGGAGAGTCTTCTCTGCGATTGATGGACGAAGGAGAGCTCTCAAGTCTACTTCGATAGGTCCATTTGTTGTTCAAGTCTTTGAGCTTGTCTTTCACGCATTTGCACCACTCTCCAAAAATGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

185

Amino Acids

21.36

Weight (kDa)

9.28

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 2 - 36 1.6e-06 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0020537)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0387391
rosa_laevigata RLG00000018080 RLG00000018082
rosa_multiflora Rmu_sc0004283.1_g000014 Rmu_sc0009639.1_g000004
rosa_rugosa Rorug02G0188900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 473
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 2 cut(s) 128, 262
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 6 cut(s) 7, 59, 233, 289, 326, 500
AluBI AGCT 3 cut(s) 373, 464, 511
AluI AGCT 3 cut(s) 373, 464, 511
Alw21I GWGCWC 1 cut(s) 466
AlwI GGATC 1 cut(s) 396
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 2 cut(s) 128, 262
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 262
AspS9I GGNCC 1 cut(s) 485
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 254
AvaII GGWCC 1 cut(s) 485
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 443
BcnI CCSGG 1 cut(s) 386
BglII AGATCT 1 cut(s) 226
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 485
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 2 cut(s) 164, 386
BpuEI CTTGAG 2 cut(s) 28, 452
BpuMI CCSGG 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 296
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 366, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 3 cut(s) 147, 369, 424
BseCI ATCGAT 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 296
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 384
BseNI ACTGG 3 cut(s) 147, 369, 424
BseXI GCAGC 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 385
BslI CCNNNNNNNGG 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 3 cut(s) 34, 226, 388
BspDI ATCGAT 1 cut(s) 64
BspPI GGATC 1 cut(s) 396
BsrI ACTGG 3 cut(s) 147, 369, 424
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 3 cut(s) 34, 226, 388
BssT1I CCWWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 152, 202
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 3 cut(s) 37, 229, 391
BstMBI GATC 3 cut(s) 34, 226, 388
BstSCI CCNGG 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 2 cut(s) 226, 388
BstYI RGATCY 2 cut(s) 226, 388
Bsu15I ATCGAT 1 cut(s) 64
BsuTUI ATCGAT 1 cut(s) 64
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 2 cut(s) 157, 362
Cfr13I GGNCC 1 cut(s) 485
ClaI ATCGAT 1 cut(s) 64
Csp6I GTAC 1 cut(s) 203
CviAII CATG 2 cut(s) 85, 395
CviJI RGCY 4 cut(s) 295, 373, 464, 511
CviKI_1 RGCY 4 cut(s) 295, 373, 464, 511
CviQI GTAC 1 cut(s) 203
DpnI GATC 3 cut(s) 36, 228, 390
DpnII GATC 3 cut(s) 34, 226, 388
Ecl136II GAGCTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 485
Eco53kI GAGCTC 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 464
EcoRI GAATTC 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 2 cut(s) 88, 398
FaiI YATR 7 cut(s) 69, 86, 121, 123, 210, 212, 396
FatI CATG 2 cut(s) 84, 394
FauNDI CATATG 1 cut(s) 210
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 1 cut(s) 371
GluI GCNGC 1 cut(s) 371
GsuI CTGGAG 2 cut(s) 164, 386
HapII CCGG 1 cut(s) 385
Hin1II CATG 2 cut(s) 88, 398
HinfI GANTC 1 cut(s) 432
HpaII CCGG 1 cut(s) 385
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 1 cut(s) 409
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 4 cut(s) 169, 181, 388, 450
HpyCH4III ACNGT 2 cut(s) 152, 202
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 3 cut(s) 88, 281, 530
HpySE526I ACGT 1 cut(s) 236
Hsp92II CATG 2 cut(s) 88, 398
Kzo9I GATC 3 cut(s) 34, 226, 388
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 6 cut(s) 128, 225, 350, 389, 398, 405
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 181
MaeII ACGT 1 cut(s) 236
MalI GATC 3 cut(s) 36, 228, 390
MboI GATC 3 cut(s) 34, 226, 388
MboII GAAGA 8 cut(s) 47, 73, 183, 221, 301, 314, 383, 427
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 2 cut(s) 226, 388
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 5 cut(s) 128, 262, 311, 334, 343
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 4 cut(s) 139, 166, 262, 392
MroXI GAANNNNTTC 1 cut(s) 262
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 66
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 334
NciI CCSGG 1 cut(s) 386
NdeI CATATG 1 cut(s) 210
NdeII GATC 3 cut(s) 34, 226, 388
NlaIII CATG 2 cut(s) 88, 398
PdmI GAANNNNTTC 1 cut(s) 262
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 466
PspPI GGNCC 1 cut(s) 485
PsuI RGATCY 2 cut(s) 226, 388
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 66
SacI GAGCTC 1 cut(s) 466
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 3 cut(s) 34, 226, 388
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 466
SetI ASST 9 cut(s) 96, 239, 244, 273, 375, 408, 466, 487, 513
SfaNI GCATC 1 cut(s) 181
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 43, 467
SmoI CTYRAG 2 cut(s) 43, 467
Sse9I AATT 5 cut(s) 128, 262, 311, 334, 343
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 152, 202
TaiI ACGT 1 cut(s) 239
TaqI TCGA 2 cut(s) 64, 479
TasI AATT 5 cut(s) 128, 262, 311, 334, 343
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 2 cut(s) 157, 369
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 4 cut(s) 39, 73, 236, 383
TspRI CASTG 2 cut(s) 157, 369
VpaK11BI GGWCC 1 cut(s) 485
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 2 cut(s) 128, 262
XmiI GTMKAC 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.