RLG00000018647

Dolichol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008091
Physical Location & Seq
Reverse (-)
33275030 .. 33287975
12946 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018647

Sequence Viewer

Length: 3447 bp
ATGGCTAAGTATGGTATTTGTCAAAATATTATTACGAGAGTCCCAACGGCACACCCAAACGCGACCGCATATTCTGCGTTGGGCGGGGGGCAGACGTTCCCACACACGCCACACGACACTCCAATTCCCGCGAAAAACACCGCCACCGCCACCGCCGCCTCTACGACGTCGTCTCCGAAAGAATCGAAAACGCCGCCGGCGGCCATGGCTTCGTTGCTGAACGGAGAGAGAGCCGTCGTGGCGCTGTTCGTCGGCCGCGTCCTCTTCTCTCTCCCGCTCTCTCTCCTCCCCCACGGCCTCGCTCTCTCCCTCCTCGCAATCTCCGCTCTCGTCCTCGACGTCCTCGCCGACTCCTCCACCACTCTCCTTTCCCAATTCAACACCAGGCCCGGTGCTTCCTCAGGTATATTATTGGGCGCCGTCACGTTGCCCACTGTCATGATCTCCAAAATGATACAGCTCTCACGACCTTTCTCACTCAATCAAGTTGCACATCAAGAGCTTGAATCTTTGACGATGCAATACTGGGCCATTTCTGCCAGTTCCTTGAGTGTACTTATATTCCTCTGCATCACTATATGGTGTGCACCTGGGAATAAGCCTCCCCCTTCTTTACGCAGTGTTTGGCATGCAATTTTAAGCTTAAGTTGCATAGTATTGCACACAGCAGTGTGTTGTGTAACTCTTGGTACATCATCTCTTACAAGCTCTGAGGCTGCATTGAAGTTATTGTGCATGCTCTGTCATGGATTGGCAGTTGTAAAATTAATTCAACATGTTTTCAGAACTTTTCCATCTTGTGCTTCGATTGGGGAAGCATGTCTAGTGGCTTCAGGTCTTGTTCTCTATTTTGGTGACATGTTGGCATATACTATTGCAAAGGTGTCTGGCTTCTCAATTAAATCAGATATGGTACAATATGGAAGTAAAACAAATGAGATAAATGTTATTATACAGGGTTTGCTGCTTGGCCTTCTTCTTTTTCCAGTGGTCTTTAAACTTGTTCTCCGAATGTGGGAATGCTCTTTCAGTGCGGCCCGCTCTGAAGTGAGGACAAACAATGAAACTTGGAGATCAGTTATATTCTTTTCGTCTCTCGGATTTATCATGATTGTGATCATTCCAGCGTGGATGCAGTTTGTTCTGGATTTTCGTATGCATCCGTTCTTATGGGTACTGTCATTTATTTTTTCGGAACCACTCAAGAGATTATCTCTGTGTGGCTACTGGGTTTGTGTGATATGTGTGTCTGTTTTGAGGTTCTACAACATTTCAAAGAACAGTAAGATAGAGAGAATTCTACTCCGAAAATACTACCATTTGATGGCTGTTTTAATGTTTGTGCCTGCTCTTATCTTCCAGCCAGAGTTTCTTGATCTAGCATTTGGTGCAGCTTTGGCAGTTTTTTTGGCATTGGAAATTGTTCGAGTATGGAGAATTTGGCCAGTGGGACAATTTATACATAAATTCATGAGTGCTTTCACCGATCATCGTGACTCAGATCTTCTTATCGTTAGCCACTTTTCACTCTTGTTGGGATGCGCTCTTCCTATTTGGATGTCTTCTGGGTATAATGATCGACCTCTATCCCCTTTTTCTGGAATTTTAAGTCTTGGAATTGGAGATACAATGGCATCAGTTGTTGGGCACAAGTACGGGGTACTCAGGTGGAGCAAAACTGGAAAGAAAACCGTTGAAGGGACTGCAGCTGGTATAACATCTGTCCTAGCTGCTTGCTCTGTTTTGCTTCCCCTTCTAGCATCTACTGGATATATTGTTACTGAGCATTGGATCTCTATTCTAGTAGCTGGGACTGTGAGTGGATTGCTGGAGGCTTACACAGCACAACTTGACAATGCTTTCATACCACTCATTTTCTACAGCCTTCTCTGTTTACTGCTGCATTGCTTTCGTTGCTTCAAGGCTTCAGTCCTTCAAGCCCACCCCGAGAGTCCCGACCAGGCGACCACGATCGTCGAGCAAGGCAGCAAGCTGGGTGGGTTCCTTTCTCTTCTTCTCTCAGAGTCTCAGAGATCGAAGGCTCCTTCTCTTCTACTCTTCTTCTTCCATTTGGGGAATCAATTGAATGTAATGCAAGCAGAGAGCTATAGCTATCTTAGAAACCCAAGCCCACCCGAAGTTTCATTCCTAGATCTGCCTCTGCGTCTCAGTCATCATCTTCATCGCCGCAACATGGCCTTCTCCAAGTTCCAGCCACTGAACGACAAGTCGCTCGTAGATTACATAAAGGCCACACCTTCCCTCTCTTCCAAGCTCAAGGATCACCTAGACGACTTGACCATCAAGGAAGTCGGTGATGGCAATCTCAATTTCGTCTTCATCGTTGTTGGTTCTCATGGCTCTTTCGTCATCAAGCAGGCTCTTCCTTACATACGTTGTATAGGTGAGTCATGGCCGATGACAAAGGAAAGAGCTTATTTTGAGGCATTGGCACTGAGAGAGCATGGTCGTTTGAGTCCTGATTATGTGCCTGAAGTTTATCATTTTGATCGGACTATGTCTTTGATTGGCATGCGTTATTTGGAACCTCCACATATTATACTGCGAAAGGGATTGATTGCTGGAATTGAGTACCCTTTGCTGGCTGACCACATGTCAGACTATATGGCAAGGACTCTGTTCTACACTTCTCTCCTTTATCGTAATACTACCGACCACAGGAAAGCTGTTGCTGAATTTTGTGGAAATGTGGAGTTATGCAGGCTCACTGAGCAAGTTGTTTTCTCTGACCCTTACAAAGTATCTGAATATAACCGCTGGACTTCTCCTTATCTTGATTCTGATGCTGAGGCTGTGCGAGAGGACAATGCTTTGAAGCTTGAAGTTGCTGAGTTAAAATCTTTGTTCTGTGAGAGAGCCCAAGCCCTAATACATGGGGATCTTCACACTGGTTCTGTAATGGTGACTCCTGATGCAACTCAAGTTATAGATCCAGAATTTGCATTTTATGGACCAATAGGTTTTGATATTGGAGCTTTTCTGGGAAACTTGATTTTGGCTTTCTTTGCACAAGATGGGCATGCTGATGAACAAAATGATCGAAAAGCGTACAAGGCTTGGATTTTGAGGATAATTCAAAATACATGGAACCTTTTCCACAGAAAATTTATCGCACTATGGGATGCGAACAAAGATGCTGGTGACGCGTATCTTCCTGCAATTTATAACAACTCTGAGCTTCAACAGCTTCTACAAGACAAATATATTAAAGAATTGCTTTATGACACCCTTGGATTTGGTGCTGCGAAAATGATAAGGAGAATTGTTGGTGTGGCACATGTCGAAGATTTTGAATCAATCACTGATGCTAGCAAGAGAGCAGGCTGTGAGCGACGGGCACTTGAGTTTGCAAAGCTGATTCTTAAGGAGAGGAGAAAATTTCAGAACATTGAGGAAGTTGTTTCAGCAGTTCAACAAATCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko01100 map00510 map01100
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1149

Amino Acids

127.58

Weight (kDa)

8.17

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APH PF01636 767 - 1003 6.1e-12 Phosphotransferase enzyme family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3186
AatII GACGTC 2 cut(s) 170, 342
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 1 cut(s) 1324
AccBSI CCGCTC 3 cut(s) 277, 326, 1041
AccII CGCG 4 cut(s) 62, 131, 258, 3167
AclWI GGATC 4 cut(s) 1799, 2289, 2907, 2945
AcoI YGGCCR 4 cut(s) 201, 253, 1442, 2414
AcsI RAATTY 8 cut(s) 1296, 1437, 1466, 1602, 2696, 2957, 3125, 3398
AcuI CTGAAG 4 cut(s) 816, 1065, 1911, 2514
AcyI GRCGYC 3 cut(s) 167, 339, 417
AfaI GTAC 8 cut(s) 555, 691, 915, 1176, 1655, 1662, 2594, 3071
AfiI CCNNNNNNNGG 7 cut(s) 1015, 1324, 1598, 1698, 2194, 2602, 2679
AflII CTTAAG 2 cut(s) 643, 3383
AflIII ACRYGT 5 cut(s) 775, 858, 2613, 3165, 3298
AhdI GACNNNNNGTC 1 cut(s) 2614
AjnI CCWGG 3 cut(s) 383, 589, 1959
AleI CACNNNNGTG 1 cut(s) 668
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 4 cut(s) 177, 1098, 2031, 2171
Alw44I GTGCAC 1 cut(s) 585
AlwI GGATC 4 cut(s) 1799, 2289, 2907, 2945
AlwNI CAGNNNCTG 1 cut(s) 2218
Ama87I CYCGRG 1 cut(s) 1946
ApaLI GTGCAC 1 cut(s) 585
ApeKI GCWGC 8 cut(s) 716, 964, 1391, 1706, 1730, 1900, 1986, 3263
ApoI RAATTY 8 cut(s) 1296, 1437, 1466, 1602, 2696, 2957, 3125, 3398
ArsI GACNNNNNNTTYG 2 cut(s) 2506, 2538
AseI ATTAAT 1 cut(s) 767
Asp700I GAANNNNTTC 2 cut(s) 1422, 3113
AspLEI GCGC 3 cut(s) 244, 419, 1544
AspS9I GGNCC 4 cut(s) 387, 528, 1036, 2972
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 7 cut(s) 866, 1474, 2276, 2327, 2417, 2935, 3173
AsuNHI GCTAGC 1 cut(s) 3329
AvaI CYCGRG 1 cut(s) 1946
AvaII GGWCC 1 cut(s) 2972
AxyI CCTNAGG 1 cut(s) 400
BaeGI GKGCMC 3 cut(s) 589, 1650, 3361
BaeI ACNNNNGTAYC 2 cut(s) 1652, 1685
BalI TGGCCA 1 cut(s) 1444
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 2881
BbsI GAAGAC 2 cut(s) 1554, 2329
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 2808
BbvI GCAGC 8 cut(s) 703, 951, 1403, 1717, 1718, 1887, 1998, 3250
BccI CCATC 5 cut(s) 802, 1318, 2309, 2312, 3029
BceAI ACGGC 4 cut(s) 63, 218, 310, 404
BcgI CGANNNNNNTGC 2 cut(s) 1892, 1926
BciT130I CCWGG 3 cut(s) 385, 591, 1961
BclI TGATCA 1 cut(s) 1116
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 4 cut(s) 177, 1098, 2031, 2171
BfaI CTAG 8 cut(s) 824, 1379, 1727, 1757, 1802, 2150, 2288, 3330
BfmI CTRYAG 3 cut(s) 1704, 1879, 2107
BfoI RGCGCY 2 cut(s) 245, 420
BfrI CTTAAG 2 cut(s) 643, 3383
BglI GCCNNNNNGGC 1 cut(s) 239
BglII AGATCT 2 cut(s) 1501, 2152
Bme1390I CCNGG 4 cut(s) 385, 390, 591, 1961
Bme18I GGWCC 1 cut(s) 2972
BmeRI GACNNNNNGTC 1 cut(s) 2614
BmeT110I CYCGRG 1 cut(s) 1946
BmgT120I GGNCC 4 cut(s) 387, 528, 1036, 2972
BmiI GGNNCC 6 cut(s) 418, 1197, 2003, 2043, 2547, 3110
BmrFI CCNGG 4 cut(s) 385, 390, 591, 1961
BmrI ACTGGG 2 cut(s) 535, 1237
BmtI GCTAGC 1 cut(s) 3333
BmuI ACTGGG 2 cut(s) 535, 1237
BpiI GAAGAC 2 cut(s) 1554, 2329
BplI GAGNNNNNCTC 2 cut(s) 1198, 1230
BpmI CTGGAG 1 cut(s) 1850
Bpu10I CCTNAGC 1 cut(s) 2808
BpuEI CTTGAG 5 cut(s) 568, 1187, 2261, 2925, 3383
BpuMI CCSGG 1 cut(s) 390
BsaBI GATNNNNATC 2 cut(s) 1115, 2322
BsaHI GRCGYC 3 cut(s) 167, 339, 417
BsaJI CCNNGG 4 cut(s) 204, 292, 590, 3250
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 990, 1020
Bsc4I CCNNNNNNNGG 7 cut(s) 1015, 1324, 1598, 1698, 2194, 2602, 2679
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 9 cut(s) 530, 540, 986, 1232, 1445, 1684, 1771, 2914, 3442
Bse21I CCTNAGG 1 cut(s) 400
Bse3DI GCAATG 1 cut(s) 1903
Bse8I GATNNNNATC 2 cut(s) 1115, 2322
BseBI CCWGG 3 cut(s) 385, 591, 1961
BseDI CCNNGG 4 cut(s) 204, 292, 590, 3250
BseGI GGATG 5 cut(s) 1137, 1159, 1544, 1563, 3148
BseJI GATNNNNATC 2 cut(s) 1115, 2322
BseLI CCNNNNNNNGG 7 cut(s) 1015, 1324, 1598, 1698, 2194, 2602, 2679
BseMI GCAATG 1 cut(s) 1903
BseNI ACTGG 9 cut(s) 530, 540, 986, 1232, 1445, 1684, 1771, 2914, 3442
BseRI GAGGAG 4 cut(s) 275, 302, 343, 3406
BseSI GKGCMC 3 cut(s) 589, 1650, 3361
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 8 cut(s) 703, 951, 1403, 1717, 1718, 1887, 1998, 3250
BseYI CCCAGC 2 cut(s) 1808, 1993
BsgI GTGCAG 1 cut(s) 1410
Bsh1236I CGCG 4 cut(s) 62, 131, 258, 3167
Bsh1285I CGRYCG 3 cut(s) 66, 256, 1974
BshNI GGYRCC 1 cut(s) 416
BsiEI CGRYCG 3 cut(s) 66, 256, 1974
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 1 cut(s) 1946
BsiSI CCGG 2 cut(s) 197, 390
BslFI GGGAC 5 cut(s) 26, 1464, 1714, 1825, 1938
BslI CCNNNNNNNGG 7 cut(s) 1015, 1324, 1598, 1698, 2194, 2602, 2679
BsmAI GTCTC 4 cut(s) 177, 1098, 2031, 2171
BsmBI CGTCTC 3 cut(s) 177, 1098, 2171
BsmFI GGGAC 5 cut(s) 26, 1464, 1714, 1825, 1938
BsmI GAATGC 1 cut(s) 1025
BsoBI CYCGRG 1 cut(s) 1946
Bsp1286I GDGCHC 4 cut(s) 589, 1650, 2881, 3361
Bsp19I CCATGG 1 cut(s) 204
BspFNI CGCG 4 cut(s) 62, 131, 258, 3167
BspHI TCATGA 3 cut(s) 438, 1107, 1470
BspLI GGNNCC 6 cut(s) 418, 1197, 2003, 2043, 2547, 3110
BspMAI CTGCAG 1 cut(s) 1708
BspOI GCTAGC 1 cut(s) 3333
BspPI GGATC 4 cut(s) 1799, 2289, 2907, 2945
BspQI GCTCTTC 2 cut(s) 1551, 2388
BspT107I GGYRCC 1 cut(s) 416
BspTI CTTAAG 2 cut(s) 643, 3383
BsrBI CCGCTC 3 cut(s) 277, 326, 1041
BsrDI GCAATG 1 cut(s) 1903
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 9 cut(s) 530, 540, 986, 1232, 1445, 1684, 1771, 2914, 3442
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 4 cut(s) 204, 292, 590, 3250
BssNI GRCGYC 3 cut(s) 167, 339, 417
BssT1I CCWWGG 2 cut(s) 204, 3250
Bst2UI CCWGG 3 cut(s) 385, 591, 1961
Bst4CI ACNGT 5 cut(s) 436, 1179, 1283, 1693, 1816
Bst6I CTCTTC 7 cut(s) 269, 1551, 2016, 2055, 2063, 2272, 2388
BstACI GRCGYC 3 cut(s) 167, 339, 417
BstAFI CTTAAG 2 cut(s) 643, 3383
BstAPI GCANNNNNTGC 2 cut(s) 74, 1388
BstDSI CCRYGG 2 cut(s) 204, 292
BstF5I GGATG 5 cut(s) 1137, 1159, 1544, 1563, 3148
BstFNI CGCG 4 cut(s) 62, 131, 258, 3167
BstH2I RGCGCY 2 cut(s) 245, 420
BstHHI GCGC 3 cut(s) 244, 419, 1544
BstMAI GTCTC 4 cut(s) 177, 1098, 2031, 2171
BstMCI CGRYCG 3 cut(s) 66, 256, 1974
BstNI CCWGG 3 cut(s) 385, 591, 1961
BstNSI RCATGY 9 cut(s) 632, 739, 779, 822, 862, 2536, 2617, 3044, 3302
BstSCI CCNGG 4 cut(s) 383, 388, 589, 1959
BstSFI CTRYAG 3 cut(s) 1704, 1879, 2107
BstSLI GKGCMC 3 cut(s) 589, 1650, 3361
BstUI CGCG 4 cut(s) 62, 131, 258, 3167
BstV1I GCAGC 8 cut(s) 703, 951, 1403, 1717, 1718, 1887, 1998, 3250
BstV2I GAAGAC 2 cut(s) 1554, 2329
BstX2I RGATCY 5 cut(s) 1501, 1791, 2152, 2899, 2950
BstYI RGATCY 5 cut(s) 1501, 1791, 2152, 2899, 2950
BstZI CGGCCG 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 400
BtgI CCRYGG 2 cut(s) 204, 292
BtgZI GCGATG 1 cut(s) 2168
BtsCI GGATG 5 cut(s) 1137, 1159, 1544, 1563, 3148
BtsI GCAGTG 2 cut(s) 625, 675
CaiI CAGNNNCTG 1 cut(s) 2218
CciI TCATGA 3 cut(s) 438, 1107, 1470
CfoI GCGC 3 cut(s) 244, 419, 1544
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 4 cut(s) 387, 528, 1036, 2972
CseI GACGC 3 cut(s) 247, 2153, 3173
Csp6I GTAC 8 cut(s) 554, 690, 914, 1175, 1654, 1661, 2593, 3070
CspCI CAANNNNNGTGG 2 cut(s) 1978, 2013
CviQI GTAC 8 cut(s) 554, 690, 914, 1175, 1654, 1661, 2593, 3070
DinI GGCGCC 1 cut(s) 418
DraI TTTAAA 1 cut(s) 997
DriI GACNNNNNGTC 1 cut(s) 2614
EaeI YGGCCR 4 cut(s) 201, 253, 1442, 2414
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 7 cut(s) 269, 1551, 2016, 2055, 2063, 2272, 2388
Eam1105I GACNNNNNGTC 1 cut(s) 2614
EarI CTCTTC 7 cut(s) 269, 1551, 2016, 2055, 2063, 2272, 2388
EclXI CGGCCG 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 204, 3250
Eco24I GRGCYC 1 cut(s) 2881
Eco47I GGWCC 1 cut(s) 2972
Eco52I CGGCCG 1 cut(s) 253
Eco57I CTGAAG 4 cut(s) 816, 1065, 1911, 2514
Eco81I CCTNAGG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 1946
EcoRI GAATTC 1 cut(s) 1296
EcoRII CCWGG 3 cut(s) 383, 589, 1959
EcoT14I CCWWGG 2 cut(s) 204, 3250
EcoT22I ATGCAT 1 cut(s) 1161
EcoT38I GRGCYC 1 cut(s) 2881
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 204, 3250
Esp3I CGTCTC 3 cut(s) 177, 1098, 2171
FalI AAGNNNNNCTT 2 cut(s) 3222, 3254
FaqI GGGAC 5 cut(s) 26, 1464, 1714, 1825, 1938
FauI CCCGC 4 cut(s) 77, 136, 282, 1046
FbaI TGATCA 1 cut(s) 1116
FokI GGATG 5 cut(s) 1144, 1146, 1551, 1570, 3155
FriOI GRGCYC 1 cut(s) 2881
FspBI CTAG 8 cut(s) 824, 1379, 1727, 1757, 1802, 2150, 2288, 3330
GlaI GCGC 3 cut(s) 243, 418, 1543
GsaI CCCAGC 2 cut(s) 1812, 1997
GsuI CTGGAG 1 cut(s) 1850
HaeII RGCGCY 2 cut(s) 245, 420
HapII CCGG 2 cut(s) 197, 390
HgaI GACGC 3 cut(s) 247, 2153, 3173
HhaI GCGC 3 cut(s) 244, 419, 1544
Hin1I GRCGYC 3 cut(s) 167, 339, 417
Hin6I GCGC 3 cut(s) 242, 417, 1542
HinP1I GCGC 3 cut(s) 242, 417, 1542
HindIII AAGCTT 2 cut(s) 640, 2837
HpaII CCGG 2 cut(s) 197, 390
HphI GGTGA 7 cut(s) 866, 1474, 2276, 2327, 2417, 2935, 3173
Hpy166II GTNNAC 3 cut(s) 554, 587, 1895
Hpy8I GTNNAC 3 cut(s) 554, 587, 1895
Hpy99I CGWCG 7 cut(s) 169, 172, 239, 254, 341, 1979, 3357
HpyCH4III ACNGT 5 cut(s) 436, 1179, 1283, 1693, 1816
HpyCH4IV ACGT 5 cut(s) 95, 167, 339, 425, 2395
HpySE526I ACGT 5 cut(s) 95, 167, 339, 425, 2395
Hsp92I GRCGYC 3 cut(s) 167, 339, 417
HspAI GCGC 3 cut(s) 242, 417, 1542
KasI GGCGCC 1 cut(s) 416
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 1116
LguI GCTCTTC 2 cut(s) 1551, 2388
LmnI GCTCC 3 cut(s) 1671, 2047, 2993
Lsp1109I GCAGC 8 cut(s) 703, 951, 1403, 1717, 1718, 1887, 1998, 3250
MaeI CTAG 8 cut(s) 824, 1379, 1727, 1757, 1802, 2150, 2288, 3330
MaeII ACGT 5 cut(s) 95, 167, 339, 425, 2395
MaeIII GTNAC 7 cut(s) 421, 679, 854, 1493, 1777, 2923, 3161
MbiI CCGCTC 3 cut(s) 277, 326, 1041
MfeI CAATTG 1 cut(s) 2081
MflI RGATCY 5 cut(s) 1501, 1791, 2152, 2899, 2950
MhlI GDGCHC 4 cut(s) 589, 1650, 2881, 3361
MlsI TGGCCA 1 cut(s) 1444
MluI ACGCGT 1 cut(s) 3165
MluNI TGGCCA 1 cut(s) 1444
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 9 cut(s) 48, 344, 1490, 1960, 2033, 2417, 2485, 2629, 2920
Mox20I TGGCCA 1 cut(s) 1444
Mph1103I ATGCAT 1 cut(s) 1161
MreI CGCCGGCG 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 196
MroXI GAANNNNTTC 2 cut(s) 1422, 3113
MscI TGGCCA 1 cut(s) 1444
MslI CAYNNNNRTG 4 cut(s) 437, 668, 1112, 3045
Msp20I TGGCCA 1 cut(s) 1444
MspA1I CMGCKG 2 cut(s) 1709, 2778
MspCI CTTAAG 2 cut(s) 643, 3383
MspI CCGG 2 cut(s) 197, 390
MspR9I CCNGG 4 cut(s) 385, 390, 591, 1961
MunI CAATTG 1 cut(s) 2081
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 3 cut(s) 385, 591, 1961
MvnI CGCG 4 cut(s) 62, 131, 258, 3167
NaeI GCCGGC 1 cut(s) 198
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 1 cut(s) 390
NcoI CCATGG 1 cut(s) 204
NgoMIV GCCGGC 1 cut(s) 196
NheI GCTAGC 1 cut(s) 3329
NlaIV GGNNCC 6 cut(s) 418, 1197, 2003, 2043, 2547, 3110
NmuCI GTSAC 5 cut(s) 421, 854, 1493, 2923, 3161
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 9 cut(s) 632, 739, 779, 822, 862, 2536, 2617, 3044, 3302
OliI CACNNNNGTG 1 cut(s) 668
PaeI GCATGC 4 cut(s) 632, 739, 2536, 3044
PagI TCATGA 3 cut(s) 438, 1107, 1470
PciI ACATGT 4 cut(s) 775, 858, 2613, 3298
PciSI GCTCTTC 2 cut(s) 1551, 2388
PcsI WCGNNNNNNNCGW 5 cut(s) 173, 255, 336, 345, 2340
PctI GAATGC 1 cut(s) 1025
PdiI GCCGGC 1 cut(s) 198
PdmI GAANNNNTTC 2 cut(s) 1422, 3113
PfeI GAWTC 6 cut(s) 182, 506, 2077, 2798, 3314, 3379
PflFI GACNNNGTC 2 cut(s) 169, 2518
PflMI CCANNNNNTGG 1 cut(s) 1324
Ple19I CGATCG 1 cut(s) 1974
PleI GAGTC 9 cut(s) 47, 344, 1490, 1959, 2032, 2416, 2484, 2629, 2920
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 9 cut(s) 47, 344, 1490, 1959, 2032, 2416, 2484, 2629, 2920
PscI ACATGT 4 cut(s) 775, 858, 2613, 3298
PshBI ATTAAT 1 cut(s) 767
PsiI TTATAA 1 cut(s) 3186
Psp6I CCWGG 3 cut(s) 383, 589, 1959
PspFI CCCAGC 2 cut(s) 1808, 1993
PspGI CCWGG 3 cut(s) 383, 589, 1959
PspN4I GGNNCC 6 cut(s) 418, 1197, 2003, 2043, 2547, 3110
PspPI GGNCC 4 cut(s) 387, 528, 1036, 2972
PstI CTGCAG 1 cut(s) 1708
PstNI CAGNNNCTG 1 cut(s) 2218
PsuI RGATCY 5 cut(s) 1501, 1791, 2152, 2899, 2950
PsyI GACNNNGTC 2 cut(s) 169, 2518
PvuI CGATCG 1 cut(s) 1974
PvuII CAGCTG 1 cut(s) 1709
RsaI GTAC 8 cut(s) 555, 691, 915, 1176, 1655, 1662, 2594, 3071
RsaNI GTAC 8 cut(s) 554, 690, 914, 1175, 1654, 1661, 2593, 3070
RseI CAYNNNNRTG 4 cut(s) 437, 668, 1112, 3045
SapI GCTCTTC 2 cut(s) 1551, 2388
Sau96I GGNCC 4 cut(s) 387, 528, 1036, 2972
SchI GAGTC 9 cut(s) 48, 344, 1490, 1960, 2033, 2417, 2485, 2629, 2920
ScrFI CCNGG 4 cut(s) 385, 390, 591, 1961
SduI GDGCHC 4 cut(s) 589, 1650, 2881, 3361
SfcI CTRYAG 3 cut(s) 1704, 1879, 2107
SfoI GGCGCC 1 cut(s) 418
SgrAI CRCCGGYG 1 cut(s) 196
SinI GGWCC 1 cut(s) 2972
SmiMI CAYNNNNRTG 4 cut(s) 437, 668, 1112, 3045
SmlI CTYRAG 7 cut(s) 547, 643, 1202, 2276, 2940, 3362, 3383
SmoI CTYRAG 7 cut(s) 547, 643, 1202, 2276, 2940, 3362, 3383
SphI GCATGC 4 cut(s) 632, 739, 2536, 3044
SspDI GGCGCC 1 cut(s) 416
SspI AATATT 1 cut(s) 28
SspMI CTAG 8 cut(s) 824, 1379, 1727, 1757, 1802, 2150, 2288, 3330
StyD4I CCNGG 4 cut(s) 383, 388, 589, 1959
StyI CCWWGG 2 cut(s) 204, 3250
TaaI ACNGT 5 cut(s) 436, 1179, 1283, 1693, 1816
TaiI ACGT 5 cut(s) 98, 170, 342, 428, 2398
TaqI TCGA 9 cut(s) 185, 336, 806, 1426, 1579, 1977, 2036, 3061, 3303
TatI WGTACW 1 cut(s) 553
TauI GCSGC 6 cut(s) 158, 196, 203, 258, 1037, 2190
TfiI GAWTC 6 cut(s) 182, 506, 2077, 2798, 3314, 3379
TseFI GTSAC 5 cut(s) 421, 854, 1493, 2923, 3161
TseI GCWGC 8 cut(s) 716, 964, 1391, 1706, 1730, 1900, 1986, 3263
Tsp45I GTSAC 5 cut(s) 421, 854, 1493, 2923, 3161
TspDTI ATGAA 7 cut(s) 1077, 1459, 1852, 2133, 2171, 2329, 3063
TspGWI ACGGA 2 cut(s) 237, 1152
Tth111I GACNNNGTC 2 cut(s) 169, 2518
Van91I CCANNNNNTGG 1 cut(s) 1324
Vha464I CTTAAG 2 cut(s) 643, 3383
VneI GTGCAC 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 2972
VspI ATTAAT 1 cut(s) 767
XapI RAATTY 8 cut(s) 1296, 1437, 1466, 1602, 2696, 2957, 3125, 3398
XceI RCATGY 9 cut(s) 632, 739, 779, 822, 862, 2536, 2617, 3044, 3302
XmnI GAANNNNTTC 2 cut(s) 1422, 3113
XspI CTAG 8 cut(s) 824, 1379, 1727, 1757, 1802, 2150, 2288, 3330
ZraI GACGTC 2 cut(s) 168, 340
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.